The aim of this study was to elucidate the optimized fabrication factors influencing the formation and properties of shellac(SHL) nanofibers loaded with an antimicrobial monolaurin(ML). The main and interaction effect...The aim of this study was to elucidate the optimized fabrication factors influencing the formation and properties of shellac(SHL) nanofibers loaded with an antimicrobial monolaurin(ML). The main and interaction effects of formulation and process parameters including SHL content(35%–40% w/w), ML content(1%–3% w/w), applied voltage(9–27 kV) and flow rate(0.4–1.2 ml/h) on the characteristic of nanofibers were investigated through a total of 19 experiments based on a full factorial design with three replicated center points. As a result, the SHL content was the major parameter affecting fiber diameter. Another response result revealed that the SHL content would be also the most significant negative impact on amount of beads. An increase in the concentration of SHL leaded to a reduction in the amount of beads. From the results of characterization study, it was proved that ML might be entrapped between the chains of SHL during the electrospinning process exhibiting an excellent encapsulation. According to the response surface area, small(?488 nm) and beadless(?0.48) fibers were obtained with the SHL and ML contents of 37.5% and 1.1% w/w respectively, at the applied voltage of 18 k V and the flow rate of 0.8 ml/h. In addition, the results of the kill-kinetic studies showed that SHL nanofibers loaded with ML exhibited an excellent antibacterial activity against Staphylococcus aureus, while Escherichia coli was less affected due to the hydrophilic structure of the its outer membrane. ML also exerted an antifun-gal activity by reducing the number of Candida albicans colonies. Based on their structural and antimicrobial properties, SHL nanofibers containing ML could be potentially used as a medicated dressing for wound treatment.展开更多
Modified coconut oil (MCO) enriched with monolaurin (ML) was prepared by the glycerolysis of coconut oil (1)Glycerolysis converts dilaurin (DL) and trilaurin (TL) into ML, the lauric acid monoglyceride. ML has been fo...Modified coconut oil (MCO) enriched with monolaurin (ML) was prepared by the glycerolysis of coconut oil (1)Glycerolysis converts dilaurin (DL) and trilaurin (TL) into ML, the lauric acid monoglyceride. ML has been found to have antiviral, antibacterial and antifungal activities (2)According to the antimicrobial activity, the glyceride composition of MCO was determined.展开更多
基金the financial support received from Silpakorn University Research and Development Institutesupported by the Higher Education Re-search Promotion and National Research University Project of Thailand,Office of the Higher Education Commission(2559A11462006)Faculty of Pharmacy,Silpakorn Univer-sity
文摘The aim of this study was to elucidate the optimized fabrication factors influencing the formation and properties of shellac(SHL) nanofibers loaded with an antimicrobial monolaurin(ML). The main and interaction effects of formulation and process parameters including SHL content(35%–40% w/w), ML content(1%–3% w/w), applied voltage(9–27 kV) and flow rate(0.4–1.2 ml/h) on the characteristic of nanofibers were investigated through a total of 19 experiments based on a full factorial design with three replicated center points. As a result, the SHL content was the major parameter affecting fiber diameter. Another response result revealed that the SHL content would be also the most significant negative impact on amount of beads. An increase in the concentration of SHL leaded to a reduction in the amount of beads. From the results of characterization study, it was proved that ML might be entrapped between the chains of SHL during the electrospinning process exhibiting an excellent encapsulation. According to the response surface area, small(?488 nm) and beadless(?0.48) fibers were obtained with the SHL and ML contents of 37.5% and 1.1% w/w respectively, at the applied voltage of 18 k V and the flow rate of 0.8 ml/h. In addition, the results of the kill-kinetic studies showed that SHL nanofibers loaded with ML exhibited an excellent antibacterial activity against Staphylococcus aureus, while Escherichia coli was less affected due to the hydrophilic structure of the its outer membrane. ML also exerted an antifun-gal activity by reducing the number of Candida albicans colonies. Based on their structural and antimicrobial properties, SHL nanofibers containing ML could be potentially used as a medicated dressing for wound treatment.
文摘Modified coconut oil (MCO) enriched with monolaurin (ML) was prepared by the glycerolysis of coconut oil (1)Glycerolysis converts dilaurin (DL) and trilaurin (TL) into ML, the lauric acid monoglyceride. ML has been found to have antiviral, antibacterial and antifungal activities (2)According to the antimicrobial activity, the glyceride composition of MCO was determined.
