Background:Effect of monochromatic green light illumination on embryo development has been reported in chickens.The avian pineal gland is an important photo-endocrine organ formed by a mediodorsal protrusion during em...Background:Effect of monochromatic green light illumination on embryo development has been reported in chickens.The avian pineal gland is an important photo-endocrine organ formed by a mediodorsal protrusion during embryonic development.However,the involvement of pineal gland in the light transduction process remains to be elucidated.In the present study,we investigated the influence of monochromatic green light on hatching time and explored the possible mechanism via pineal function.Results:A total of 600 eggs of White Leghorn(Shaver strain)were incubated under photoperiods of either 12 h of light and 12 h of darkness using monochromatic green light(12L:12D group)or 24 h of darkness(0L:24D group)for 18 d.Compared to 0L:24D group,the green light stimulation shortened the hatching time without extending the hatch window or impairing hatchability.The liver of embryos incubated in the 12L:12D light condition was heavier than those of the 0L:24D group on d 21 post incubation which may be linked to the observed increase in the serum concentration of insulin-like growth factor 1(IGF-1);primary secretion of the liver.Histological structure analysis of pineal gland demonstrated that the light stimulation increased follicle area,wall thickness and lumen area on d 10 and d 12 post incubation.Rhythmic function analysis demonstrated that three clock related genes(brain and muscle ARNT-like-1,BMAL1;circadian locomotor output cycles kaput,CLOCK;and cryptochrome-1,CRY1)and a melatonin rate-limiting enzyme related gene(arylalkylamine N-acetyltransferase,AANAT)were rhythmically expressed in the pineal gland of the 12L:12D group,but not in the 0L:24D group.Simultaneously,the light stimulation also increased the concentration of melatonin(MT),which was linked to hepatocyte proliferation and IGF-1 secretion in previous studies.Conclusions:The 12L:12D monochromatic green light stimulation during incubation shortened hatching time without impairing hatching performance.Pineal gland’s early histological development and maturation of its rhythmic function were accelerated by the light stimulation.It may be the key organ in the photo-endocrine axis that regulates embryo development,and the potential mechanism could be through enhanced secretion of MT in the 12L:12D group which promotes the secretion of IGF-1.展开更多
Illuminating conditions are crucial factors affecting the microalgal growth and biosynthesis.The effects of illuminating spectral quality on the growth and bio-component production of Nannochloris oculata were investi...Illuminating conditions are crucial factors affecting the microalgal growth and biosynthesis.The effects of illuminating spectral quality on the growth and bio-component production of Nannochloris oculata were investigated.The results indicated that a high proportion of Red-light enhanced the pigments and carbohydrate production but reduced those of the biomass and lipid.Mono-chromatic Blue-light has advantageous effects on lipid production compared with Red-and White-light.The optimal light spectrum for the protein production was the combination of Red-and Blue-light at a ratio of 4:3 or 5:2.Among the seven fatty acids identified from N.oculata,the contents of C16:0,C18:0,and C18:3(n-3)in the lipid were inhibited by the increased proportion of Red-light while those of C18:2(n-9),C16:2(n-6),and C20:0 were inhibited by Blue-light.Monochromatic Red-and Blue-light and their com-binations were proposed as a promising illuminating strategy for the large-scale cultivation aiming for various bio-components.展开更多
AIMTo expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor...AIMTo expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-β1 (TGF-β1) expression.METHODSThree groups of rat retinal Müller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF-β1 mRNA expression, protein levels and fluorescence intensity of the Müller cells were analyzed.RESULTSThe bFGF mRNA expression and protein levels were significantly upregulated in Müller cells in all three experimental groups compared with the control group (P<0.05), while that of TGF-β1 was downregulated (P<0.05). Also, bFGF expression was positively correlated, but TGF-β1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24h group. The changes in bFGF and TGF-β1 fluorescence intensity were highest in the 24h group, and significant differences were observed among the experimental groups (P<0.05).CONCLUSIONThe expressions of bFGF and TGF-β1 changed in a time-dependent manner in Müller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. Müller cells might play a role in the development of myopia by increasing bFGF expression or decreasing TGF-β1 expression. Changes in cytokine expression in retinal Müller cells may affect monochromatic light-induced myopia.展开更多
AIM: To investigate the value of computed tomography(CT) spectral imaging in the evaluation of intestinal hemorrhage.METHODS: Seven blood flow rates were simulated in vitro.Energy spectral CT and mixed-energy CT scans...AIM: To investigate the value of computed tomography(CT) spectral imaging in the evaluation of intestinal hemorrhage.METHODS: Seven blood flow rates were simulated in vitro.Energy spectral CT and mixed-energy CT scanswere performed for each rate(0.5,0.4,0.3,0.2,0.1,0.05 and 0.025 m L/min).The detection rates and the contrast-to-noise ratios(CNRs) of the contrast agent extravasation regions were compared between the two scanning methods in the arterial phase(AP) and the portal venous phase(PVP).Comparisons of the CNR values between the PVP and the AP were made for each energy level and carried out using a completely random t test.A χ2 test was used to compare the detection rates obtained from the two scanning methods.RESULTS: The total detection rates for energy spectral CT and mixed-energy CT in the AP were 88.57%(31/35) and 65.71%(23/35),respectively,and the difference was significant(χ2 = 5.185,P = 0.023); the total detection rates in the PVP were 100.00%(35/35) and 91.4%(32/35),respectively,and the difference was not significant(χ2 = 1.393,P = 0.238).In the AP,the CNR of the contrast agent extravasation regions was 3.58 ± 2.09 on the mixed-energy CT images,but the CNRs were 8.78 ± 7.21 and 8.83 ± 6.75 at 50 and 60 keV,respectively,on the single-energy CT images,which were significantly different(3.58 ± 2.09 vs 8.78 ± 7.21,P = 0.031; 3.58 ± 2.09 vs 8.83 ± 6.75,P = 0.029).In the PVP,the differences between the CNRs at 40,50 and 60 keV different monochromatic energy levels and the polychromatic energy images were significant(19.35 ± 10.89 vs 11.68 ± 6.38,P = 0.010; 20.82 ± 11.26 vs 11.68 ± 6.38,P = 0.001; 20.63 ± 10.07 vs 11.68 ± 6.38,P = 0.001).The CNRs at the different energy levels in the AP and the PVP were significantly different(t =-2.415,-2.380,-2.575,-2.762,-2.945,-3.157,-3.996 and-3.189).CONCLUSION: Monochromatic energy imaging spectral CT is superior to polychromatic energy images for the detection of intestinal hemorrhage,and the detection was easier in the PVP compared with the AP.展开更多
Monochromatic x-ray imaging is an essential method for plasma diagnostics related to density information.Large-field high-resolution monochromatic imaging of a He-like iron(Fe XXV)Kαcharacteristic line(6.701 keV)for ...Monochromatic x-ray imaging is an essential method for plasma diagnostics related to density information.Large-field high-resolution monochromatic imaging of a He-like iron(Fe XXV)Kαcharacteristic line(6.701 keV)for laser plasma diagnostics was achieved using a developed toroidal crystal x-ray imager.A high-index crystal orientation Ge(531)wafer with a Bragg angle of 75.37°and the toroidal substrate were selected to obtain sufficient diffraction efficiency and compensate for astigmatism under oblique incidence.A precise offline assembly method of the toroidal crystal imager based on energy substitution was proposed,and a spatial resolution of 3-7μm was obtained by toroidal crystal imaging of a 600 line-pairs/inch Au grid within an object field of view larger than 1.0 mm.The toroidal crystal x-ray imager has been successfully tested via side-on backlight imaging experiments of the sinusoidal modulation target and a 1000 line-pairs/inch Au grid with a linewidth of 5μm using an online alignment method based on dual positioning balls to indicate the target and backlighter.This paper describes the optical design,adjustment method,and experimental results of a toroidal crystal system in a laboratory and laser facility.展开更多
Effects of light intensity and quality of three kinds of LED monochromatic lights (blue, green, and red) on the growth of Skeletonema costatum are investigated in batch culture conditions. Seven light intensities (20,...Effects of light intensity and quality of three kinds of LED monochromatic lights (blue, green, and red) on the growth of Skeletonema costatum are investigated in batch culture conditions. Seven light intensities (20, 30, 40, 45, 50, 60 and 80 μmol·m-2·s-1) are used to evaluate the specific growth rate, spectrum absorption coefficient and saturated light intensity of LED monochromatic light. Results show that the growth rates of Skeletonema costatum increase with the enhanced light intensity;however, the light level beyond the saturation light intensity inhibited the growth of Skeletonema costatum. Compared with red and green light, the growth rate of Skeletonema costatum under blue light is higher within saturated light intensity, and saturated light intensity of LED monochromatic light is lower under blue light and higher under green light. It is concluded that under different monochromatic light, the saturated light intensity decreases and the growth rate increases with the increasing of spectrum absorption coefficient.展开更多
Similarity solution for a spherical shock wave with or without gravitational field in a dusty gas is studied under the action of monochromatic radiation. It is supposed that dusty gas be a mixture of perfect gas and m...Similarity solution for a spherical shock wave with or without gravitational field in a dusty gas is studied under the action of monochromatic radiation. It is supposed that dusty gas be a mixture of perfect gas and micro solid particles. Equilibrium flow condition is supposed to be maintained and energy is varying which is continuously supplied by inner expanding surface. It is found that similarity solution exists under the constant initial density. The comparison between the solutions obtained in gravitating and non-gravitating medium is done. It is found that the shock strength increases with an increase in gravitational parameter or ratio of the density of solid particles to the initial density of the gas, whereas an increase in the radiation parameter has decaying effect on the shock waves.展开更多
A self-similar flow behind a cylindrical shock wave is studied under the action of monochromatic radiation in a rotational axisymmetric dusty gas. The dusty gas is taken to be a mixture of small solid particles and pe...A self-similar flow behind a cylindrical shock wave is studied under the action of monochromatic radiation in a rotational axisymmetric dusty gas. The dusty gas is taken to be a mixture of small solid particles and perfect gas,and solid particles are continuously distributed in the mixture. The similarity solutions are obtained and the effects of the variation of the radiation parameter, the ratio of the density of solid particles to the initial density of the gas, the mass concentration of solid particles in the mixture and the index for the time dependent energy law are investigated.It is observed that an increase in the radiation parameter has decaying effect on the shock waves; whereas the shock strength increases with an increase in the ratio of the density of solid particles to the initial density of the gas or the index for the time dependent energy law. Also, it is found that an increase in the radiation parameter has effect to decrease the flow variables except the density and the azimuthal component of fluid velocity. A comparison is also made between rotating and non-rotating cases.展开更多
Bollobas and Gyarfas conjectured that for n 〉 4（k - 1） every 2-edge-coloring of Kn contains a monochromatic k-connected subgraph with at least n - 2k ＋ 2 vertices. Liu, et al. proved that the conjecture holds when...Bollobas and Gyarfas conjectured that for n 〉 4（k - 1） every 2-edge-coloring of Kn contains a monochromatic k-connected subgraph with at least n - 2k ＋ 2 vertices. Liu, et al. proved that the conjecture holds when n 〉 13k - 15. In this note, we characterize all the 2-edge-colorings of Kn where each monochromatic k-connected subgraph has at most n - 2k ＋ 2 vertices for n ≥ 13k - 15.展开更多
Energy determines the ability of matter to work. However, in the given environment, the real usefulness to perform work is determined by exergy. This study covers not only solar, but also any monochromatic thermal rad...Energy determines the ability of matter to work. However, in the given environment, the real usefulness to perform work is determined by exergy. This study covers not only solar, but also any monochromatic thermal radiation. The value of such radiation was determined by its exergy and the ratio of its exergy-to-energy. A novelty in this work is to demonstrate by means of exergy that the usefulness of thermal polychromatic radiation can be increased by its dispersion to monochromatic radiation. This effect is the greater, the lower the temperature of the radiation. Analogies of this effect to the exergetic effect of gas separation have been indicated. The effect of the increase in exergy in the process of radiation dispersion was interpreted by means of a cylinder-piston system that explains this effect with the influence of environmental radiation. The concept of quasi-monochromatic and cumulated radiation was introduced into dispersion considerations and the change in the energetic, entropic and environmental components of the exergy of radiation beams was analyzed. Considerations were illustrated with appropriate examples of calculations considering dispersion of high-temperature radiation, such as extraterrestrial solar radiation and dispersion of low-temperature radiation from water vapor.展开更多
A probabilistic formalism, relying on Bayes’ theorem and linear Gaussian inversion, is adapted, so that a monochromatic problem can be investigated. The formalism enables an objective test in probabilistic terms of t...A probabilistic formalism, relying on Bayes’ theorem and linear Gaussian inversion, is adapted, so that a monochromatic problem can be investigated. The formalism enables an objective test in probabilistic terms of the quantities and model concepts involved in the problem at hand. With this formalism, an amplitude (linear parameter), a frequency (non-linear parameter) and a hyperparameter of the Gaussian amplitude prior are inferred jointly given simulated data sets with Gaussian noise contributions. For the amplitude, an analytical normal posterior follows which is conditional on the frequency and the hyperparameter. The remaining posterior estimates the frequency with an uncertainty of MHz, while the convolution of a standard approach would achieve an uncertainty of some GHz. This improvement in the estimation is investigated analytically and numerically, revealing for instance the positive effect of a high signal-to-noise ratio and/or a large number of data points. As a fixed choice of the hyperparameter imposes certain results on the amplitude and frequency, this parameter is estimated and, thus, tested for plausibility as well. From abstract point of view, the model posterior is investigated as well.展开更多
Objective: Computed tomography (CT)-based attenuation correction (CTAC) offers the clear benefit of reliable reconstruction of single-photon emission computed tomography (SPECT) images through its ability to achieve o...Objective: Computed tomography (CT)-based attenuation correction (CTAC) offers the clear benefit of reliable reconstruction of single-photon emission computed tomography (SPECT) images through its ability to achieve object-specific attenuation maps, but artifacts from dense materials often deteriorate CTAC performance. Therefore, we investigate the feasibility of CTAC in the presence of dense materials using dual-energy virtual monochromatic CT data. Methods: A sodium pertechnetate-filled cylindrical uniform phantom, with a pair of undiluted iodine syringes attached, is scanned with a dual-source CT scanner to obtain both single-energy (120 kVp) polychromatic and dual-energy (80 kVp/140 kVp with tin filtering) virtual monochromatic CT images. The single-energy and the dual-energy CT images are then converted to attenuation maps at 141 keV. SPECT images are reconstructed from 99mTc emission data of the phantom using each single-energy and dual-energy attenuation map and incorporating CTAC procedure. A region-of-in- terest analysis is performed to quantitatively compare the attenuation maps between the single-energy and the dual-energy techniques, each at an iodine-free position and a position adjacent to the iodine solutions. Results: At the iodine-free position, the phantom provides a uniform distribution of attenuation maps in both the single-energy and the dual-energy techniques. In the presence of adjacent iodine solutions, however, severe artifacts appeare in the single-energy CT images. These artifacts make attenuation values fluctuate, resulting in erroneous pixel values in the CTAC SPECT images. In contrast, dual-energy CT strongly suppresses the artifacts and hence improves the uniformity of the attenuation maps and the resultant SPECT images. Conclusions: Dual-energy CT with virtual monochromatic reconstruction has the potential to substantially reduce artifacts arising from dense materials. It has the potential to improve the accuracy of attenuation maps and the resultant CTAC SPECT images.展开更多
Space scientific exploration is rapidly becoming the primary battlefield for humankind to explore the universe.Countries worldwide have launched numerous space exploration satellites.Accurate calibration of the detect...Space scientific exploration is rapidly becoming the primary battlefield for humankind to explore the universe.Countries worldwide have launched numerous space exploration satellites.Accurate calibration of the detectors on the ground is a crucial element for space science satellites to obtain observational results.For the purpose of providing calibration for various satellite-borne detectors,multiple monochromatic X-rays facilities have been built at the National Institute of Metrology,P.R.China(NIM).These facilities mainly pertain to grating diffraction and Bragg diffraction,and the energy range of the produced monochromatic X-rays is 0.218–301 ke V.These facilities have a high performance in terms of energy stability,monochromaticity,and flux stability.The monochromaticity was greater than 3.0%.The energy stability of the facility is 0.02%at 25 ke V over 8 h,and the flux stability was within 1.0%at 25 ke V over 8 h.Calibration experiments on the properties of satellite-borne detectors,such as energy linearity,energy resolution,detection efficiency,and temperature response,can be conducted at the facilities.Thus far,the calibration of two satellites has been completed by the authors,and the work on three other satellites is in progress.This study will contribute to the advancement of X-ray astronomy the development of Chinese space science.展开更多
In this study,a toroidal quartz(2023)crystal is designed for monochromatic X-ray imaging at 72.3◦.The designed crystal produces excellent images of a laser-produced plasma emitting He-like Ti X-rays at 4.75 keV.Based ...In this study,a toroidal quartz(2023)crystal is designed for monochromatic X-ray imaging at 72.3◦.The designed crystal produces excellent images of a laser-produced plasma emitting He-like Ti X-rays at 4.75 keV.Based on the simulations,the imaging resolutions of the spherical and toroidal crystals in the sagittal direction are found to be 15 and 5μm,respectively.Moreover,the simulation results show that a higher resolution image of the source can be obtained by using a toroidal crystal.An X-ray backlight imaging experiment is conducted using 4.75 keV He-like Ti X-rays,a 3×3 metal grid,an imaging plate and a toroidal quartz crystal with a lattice constant of 2d=0.2749 nm.The meridional and sagittal radii of the toroidalα-quartz crystal are 295.6 and 268.5 mm,respectively.A highly resolved image of the microgrid,with a spatial resolution of 10μm,is obtained in the experiment.By using similar toroidal crystal designs,the application of a spatially resolved spectrometer with high-resolution X-ray imaging ability is capable of providing imaging data with the same magnification ratio in the sagittal and meridional planes.展开更多
Temperature and density asymmetry diagnosis is critical to advance inertial confinement fusion(ICF) science. A multimonochromatic x-ray imager, MMI, records the spectral signature from an ICF implosion core with time ...Temperature and density asymmetry diagnosis is critical to advance inertial confinement fusion(ICF) science. A multimonochromatic x-ray imager, MMI, records the spectral signature from an ICF implosion core with time resolution, 2D spatial resolution and spectral resolution. While narrow-band images and 2D space-resolved spectra from the MMI data constrain the temperature and the density spatial structure of the core, the accuracy of the images and the spectra highly depends on the quality of the MMI data and the processing tools. Here, we synthetically investigate the criterion for reliable MMI diagnostics and its effects on the accuracy of the reconstructed images. The pinhole array tilt determines the object spatial sampling efficiency and the minimum reconstruction width, w. When the spectral width associated with w is significantly narrower than the spectral linewidth, the line images reconstructed from the MMI data become reliable. The MMI setup has to be optimized for every application to meet this criterion for reliable ICF diagnostics.展开更多
Measuring the temperature and deformation synchronously at elevated temperatures is technically challenging and has become a major concern in the evaluation of mechanical properties. In this study, a simple, easy-to-i...Measuring the temperature and deformation synchronously at elevated temperatures is technically challenging and has become a major concern in the evaluation of mechanical properties. In this study, a simple, easy-to-implement, yet effective monochromatic pyrometry is established for non-contact and full-field temperature measurements, which can significantly reduce the error caused by the camera’s channel crosstalk that commonly occurs in the existing improved two-color method. In addition, hightemperature digital image correlation, combined with band-pass filtering and monochromatic illumination, is applied for deformation measurement. Subsequently, an experimental system was set up to validate the accuracy of the proposed method,which consists of a CCD camera for image capturing, a blue bandpass filter for radiation suppression, blue light irradiation for light compensation, and an infrared pyrometer for temperature recording. The results of the thermal heating experiment on the C/SiC sample proved that the selection of camera channel R in monochromatic pyrometry can reduce the error by channel crosstalk,and the proposed method is applicable for synchronous measurement of temperature and deformation.展开更多
We report for the first time the theoretical analysis and experimental results of a white-light reconstructed monochromatic 3-D image synthesizing tomograms by multiple rainbow holo-graphy with vertical-area partition...We report for the first time the theoretical analysis and experimental results of a white-light reconstructed monochromatic 3-D image synthesizing tomograms by multiple rainbow holo-graphy with vertical-area partition (VAP) approach. The theoretical and experimental results show that 3-D monochromatic image can be synthesized by recording the master hologram by VAP ap-proach without any distortions either in gray scale or in geometrical position. A 3-D monochromatic image synthesized from a series of medical tomograms is presented in this paper for the first time.展开更多
A vertex-colored path P is rainbow if its internal vertices have distinct colors;whereas P is monochromatic if its internal vertices are colored the same.For a vertex-colored connected graph G,the rainbow vertex-conne...A vertex-colored path P is rainbow if its internal vertices have distinct colors;whereas P is monochromatic if its internal vertices are colored the same.For a vertex-colored connected graph G,the rainbow vertex-connection number rvc(G)is the minimum number of colors used such that there is a rainbow path joining any two vertices of G;whereas the monochromatic vertex-connection number mvc(G)is the maximum number of colors used such that any two vertices of G are connected by a monochromatic path.These two opposite concepts are the vertex-versions of rainbow connection number rc(G)and monochromatic connection number mc(G)respectively.The study on rc(G)and mc(G)of random graphs drew much attention,and there are few results on the rainbow and monochromatic vertex-connection numbers.In this paper,we consider these two vertex-connection numbers of random graphs and establish sharp threshold functions for them,respectively.展开更多
The measurement of X-ray continuous emission from laser-driven plasma was achieved through multiple monochromatic imaging utilizing a multilayer mirror array.This methodology was exemplified by the development of an e...The measurement of X-ray continuous emission from laser-driven plasma was achieved through multiple monochromatic imaging utilizing a multilayer mirror array.This methodology was exemplified by the development of an eight-channel X-ray imaging system,capable of operating in the energy range of several ke V with a spatial resolution of approximately3 μm.By integrating this system with a streak camera,the temperature and trajectory of imploding capsules were successfully measured at the kJ-class Shenguang III prototype laser facility.This approach provides a synchronous diagnostic method for the spatial,temporal and spectral analysis of laser-driven plasma,characterized by its high efficiency and resolution.展开更多
An application of the optical pyrometer is studied for measuring monochromatic emissivities of cement clinker with various Fe2O3 content.The idea of using “brightness temperature”is introduced into the emissivity me...An application of the optical pyrometer is studied for measuring monochromatic emissivities of cement clinker with various Fe2O3 content.The idea of using “brightness temperature”is introduced into the emissivity measurement. In this method, there is no need for measuring an actual temperature of sample surfaces, only with determining both brightness temperatures of a sample and a blackbody can the required emissivity be evaluated according to Wien’s radiation law. In practice, the cement clinker is regarded as a greybody, the monochromatic emissivity is approximately equal to the total emissivity, so a single-colour optical pyrometer is applied for this purpose. Test measurements are carried out on 10 kinds of cement clinkers. Experimental data are treated by the least square method. As a result, the emissivity variation with temperature at a certain Fe2O3 content is quite well represented by εn=α+bT. Furthermore, this work first reported that the emissivities of cement clinker change considerably with Fe2O3 contents. In multiple cement production this conclusion is very important.展开更多
基金Financial support of this study was provided by The National Key Research and Development Program of China(grant number 2016YFD0500502)China Agriculture Research Systems(grant number CARS-40)+1 种基金Fundamental Research Funds for Central Non-profit Scientific Institution(grant number 2018-YWF-YB-20)Agricultural Science and Technology Innovation Program(grant number ASTIP-IAS04).
文摘Background:Effect of monochromatic green light illumination on embryo development has been reported in chickens.The avian pineal gland is an important photo-endocrine organ formed by a mediodorsal protrusion during embryonic development.However,the involvement of pineal gland in the light transduction process remains to be elucidated.In the present study,we investigated the influence of monochromatic green light on hatching time and explored the possible mechanism via pineal function.Results:A total of 600 eggs of White Leghorn(Shaver strain)were incubated under photoperiods of either 12 h of light and 12 h of darkness using monochromatic green light(12L:12D group)or 24 h of darkness(0L:24D group)for 18 d.Compared to 0L:24D group,the green light stimulation shortened the hatching time without extending the hatch window or impairing hatchability.The liver of embryos incubated in the 12L:12D light condition was heavier than those of the 0L:24D group on d 21 post incubation which may be linked to the observed increase in the serum concentration of insulin-like growth factor 1(IGF-1);primary secretion of the liver.Histological structure analysis of pineal gland demonstrated that the light stimulation increased follicle area,wall thickness and lumen area on d 10 and d 12 post incubation.Rhythmic function analysis demonstrated that three clock related genes(brain and muscle ARNT-like-1,BMAL1;circadian locomotor output cycles kaput,CLOCK;and cryptochrome-1,CRY1)and a melatonin rate-limiting enzyme related gene(arylalkylamine N-acetyltransferase,AANAT)were rhythmically expressed in the pineal gland of the 12L:12D group,but not in the 0L:24D group.Simultaneously,the light stimulation also increased the concentration of melatonin(MT),which was linked to hepatocyte proliferation and IGF-1 secretion in previous studies.Conclusions:The 12L:12D monochromatic green light stimulation during incubation shortened hatching time without impairing hatching performance.Pineal gland’s early histological development and maturation of its rhythmic function were accelerated by the light stimulation.It may be the key organ in the photo-endocrine axis that regulates embryo development,and the potential mechanism could be through enhanced secretion of MT in the 12L:12D group which promotes the secretion of IGF-1.
基金supported by the National Natural Sci-ence Foundation of China(Nos.41776156 and 42177459)the Open-End Funds of Jiangsu Key Laboratory of Ma-rine Bioresources and Environment(No.SH20201206).
文摘Illuminating conditions are crucial factors affecting the microalgal growth and biosynthesis.The effects of illuminating spectral quality on the growth and bio-component production of Nannochloris oculata were investigated.The results indicated that a high proportion of Red-light enhanced the pigments and carbohydrate production but reduced those of the biomass and lipid.Mono-chromatic Blue-light has advantageous effects on lipid production compared with Red-and White-light.The optimal light spectrum for the protein production was the combination of Red-and Blue-light at a ratio of 4:3 or 5:2.Among the seven fatty acids identified from N.oculata,the contents of C16:0,C18:0,and C18:3(n-3)in the lipid were inhibited by the increased proportion of Red-light while those of C18:2(n-9),C16:2(n-6),and C20:0 were inhibited by Blue-light.Monochromatic Red-and Blue-light and their com-binations were proposed as a promising illuminating strategy for the large-scale cultivation aiming for various bio-components.
文摘AIMTo expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-β1 (TGF-β1) expression.METHODSThree groups of rat retinal Müller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF-β1 mRNA expression, protein levels and fluorescence intensity of the Müller cells were analyzed.RESULTSThe bFGF mRNA expression and protein levels were significantly upregulated in Müller cells in all three experimental groups compared with the control group (P<0.05), while that of TGF-β1 was downregulated (P<0.05). Also, bFGF expression was positively correlated, but TGF-β1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24h group. The changes in bFGF and TGF-β1 fluorescence intensity were highest in the 24h group, and significant differences were observed among the experimental groups (P<0.05).CONCLUSIONThe expressions of bFGF and TGF-β1 changed in a time-dependent manner in Müller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. Müller cells might play a role in the development of myopia by increasing bFGF expression or decreasing TGF-β1 expression. Changes in cytokine expression in retinal Müller cells may affect monochromatic light-induced myopia.
文摘AIM: To investigate the value of computed tomography(CT) spectral imaging in the evaluation of intestinal hemorrhage.METHODS: Seven blood flow rates were simulated in vitro.Energy spectral CT and mixed-energy CT scanswere performed for each rate(0.5,0.4,0.3,0.2,0.1,0.05 and 0.025 m L/min).The detection rates and the contrast-to-noise ratios(CNRs) of the contrast agent extravasation regions were compared between the two scanning methods in the arterial phase(AP) and the portal venous phase(PVP).Comparisons of the CNR values between the PVP and the AP were made for each energy level and carried out using a completely random t test.A χ2 test was used to compare the detection rates obtained from the two scanning methods.RESULTS: The total detection rates for energy spectral CT and mixed-energy CT in the AP were 88.57%(31/35) and 65.71%(23/35),respectively,and the difference was significant(χ2 = 5.185,P = 0.023); the total detection rates in the PVP were 100.00%(35/35) and 91.4%(32/35),respectively,and the difference was not significant(χ2 = 1.393,P = 0.238).In the AP,the CNR of the contrast agent extravasation regions was 3.58 ± 2.09 on the mixed-energy CT images,but the CNRs were 8.78 ± 7.21 and 8.83 ± 6.75 at 50 and 60 keV,respectively,on the single-energy CT images,which were significantly different(3.58 ± 2.09 vs 8.78 ± 7.21,P = 0.031; 3.58 ± 2.09 vs 8.83 ± 6.75,P = 0.029).In the PVP,the differences between the CNRs at 40,50 and 60 keV different monochromatic energy levels and the polychromatic energy images were significant(19.35 ± 10.89 vs 11.68 ± 6.38,P = 0.010; 20.82 ± 11.26 vs 11.68 ± 6.38,P = 0.001; 20.63 ± 10.07 vs 11.68 ± 6.38,P = 0.001).The CNRs at the different energy levels in the AP and the PVP were significantly different(t =-2.415,-2.380,-2.575,-2.762,-2.945,-3.157,-3.996 and-3.189).CONCLUSION: Monochromatic energy imaging spectral CT is superior to polychromatic energy images for the detection of intestinal hemorrhage,and the detection was easier in the PVP compared with the AP.
基金National Natural Science Foundation of China(No.11805212)National Key Research and Development Program of China(No.2019YFE03080200)。
文摘Monochromatic x-ray imaging is an essential method for plasma diagnostics related to density information.Large-field high-resolution monochromatic imaging of a He-like iron(Fe XXV)Kαcharacteristic line(6.701 keV)for laser plasma diagnostics was achieved using a developed toroidal crystal x-ray imager.A high-index crystal orientation Ge(531)wafer with a Bragg angle of 75.37°and the toroidal substrate were selected to obtain sufficient diffraction efficiency and compensate for astigmatism under oblique incidence.A precise offline assembly method of the toroidal crystal imager based on energy substitution was proposed,and a spatial resolution of 3-7μm was obtained by toroidal crystal imaging of a 600 line-pairs/inch Au grid within an object field of view larger than 1.0 mm.The toroidal crystal x-ray imager has been successfully tested via side-on backlight imaging experiments of the sinusoidal modulation target and a 1000 line-pairs/inch Au grid with a linewidth of 5μm using an online alignment method based on dual positioning balls to indicate the target and backlighter.This paper describes the optical design,adjustment method,and experimental results of a toroidal crystal system in a laboratory and laser facility.
文摘Effects of light intensity and quality of three kinds of LED monochromatic lights (blue, green, and red) on the growth of Skeletonema costatum are investigated in batch culture conditions. Seven light intensities (20, 30, 40, 45, 50, 60 and 80 μmol·m-2·s-1) are used to evaluate the specific growth rate, spectrum absorption coefficient and saturated light intensity of LED monochromatic light. Results show that the growth rates of Skeletonema costatum increase with the enhanced light intensity;however, the light level beyond the saturation light intensity inhibited the growth of Skeletonema costatum. Compared with red and green light, the growth rate of Skeletonema costatum under blue light is higher within saturated light intensity, and saturated light intensity of LED monochromatic light is lower under blue light and higher under green light. It is concluded that under different monochromatic light, the saturated light intensity decreases and the growth rate increases with the increasing of spectrum absorption coefficient.
文摘Similarity solution for a spherical shock wave with or without gravitational field in a dusty gas is studied under the action of monochromatic radiation. It is supposed that dusty gas be a mixture of perfect gas and micro solid particles. Equilibrium flow condition is supposed to be maintained and energy is varying which is continuously supplied by inner expanding surface. It is found that similarity solution exists under the constant initial density. The comparison between the solutions obtained in gravitating and non-gravitating medium is done. It is found that the shock strength increases with an increase in gravitational parameter or ratio of the density of solid particles to the initial density of the gas, whereas an increase in the radiation parameter has decaying effect on the shock waves.
文摘A self-similar flow behind a cylindrical shock wave is studied under the action of monochromatic radiation in a rotational axisymmetric dusty gas. The dusty gas is taken to be a mixture of small solid particles and perfect gas,and solid particles are continuously distributed in the mixture. The similarity solutions are obtained and the effects of the variation of the radiation parameter, the ratio of the density of solid particles to the initial density of the gas, the mass concentration of solid particles in the mixture and the index for the time dependent energy law are investigated.It is observed that an increase in the radiation parameter has decaying effect on the shock waves; whereas the shock strength increases with an increase in the ratio of the density of solid particles to the initial density of the gas or the index for the time dependent energy law. Also, it is found that an increase in the radiation parameter has effect to decrease the flow variables except the density and the azimuthal component of fluid velocity. A comparison is also made between rotating and non-rotating cases.
基金Supported by the National Natural Science Foundation of China(10701065 and 11101378)Zhejiang Provincial Natural Science Foundation(LY14A010009)
文摘Bollobas and Gyarfas conjectured that for n 〉 4（k - 1） every 2-edge-coloring of Kn contains a monochromatic k-connected subgraph with at least n - 2k ＋ 2 vertices. Liu, et al. proved that the conjecture holds when n 〉 13k - 15. In this note, we characterize all the 2-edge-colorings of Kn where each monochromatic k-connected subgraph has at most n - 2k ＋ 2 vertices for n ≥ 13k - 15.
文摘Energy determines the ability of matter to work. However, in the given environment, the real usefulness to perform work is determined by exergy. This study covers not only solar, but also any monochromatic thermal radiation. The value of such radiation was determined by its exergy and the ratio of its exergy-to-energy. A novelty in this work is to demonstrate by means of exergy that the usefulness of thermal polychromatic radiation can be increased by its dispersion to monochromatic radiation. This effect is the greater, the lower the temperature of the radiation. Analogies of this effect to the exergetic effect of gas separation have been indicated. The effect of the increase in exergy in the process of radiation dispersion was interpreted by means of a cylinder-piston system that explains this effect with the influence of environmental radiation. The concept of quasi-monochromatic and cumulated radiation was introduced into dispersion considerations and the change in the energetic, entropic and environmental components of the exergy of radiation beams was analyzed. Considerations were illustrated with appropriate examples of calculations considering dispersion of high-temperature radiation, such as extraterrestrial solar radiation and dispersion of low-temperature radiation from water vapor.
文摘A probabilistic formalism, relying on Bayes’ theorem and linear Gaussian inversion, is adapted, so that a monochromatic problem can be investigated. The formalism enables an objective test in probabilistic terms of the quantities and model concepts involved in the problem at hand. With this formalism, an amplitude (linear parameter), a frequency (non-linear parameter) and a hyperparameter of the Gaussian amplitude prior are inferred jointly given simulated data sets with Gaussian noise contributions. For the amplitude, an analytical normal posterior follows which is conditional on the frequency and the hyperparameter. The remaining posterior estimates the frequency with an uncertainty of MHz, while the convolution of a standard approach would achieve an uncertainty of some GHz. This improvement in the estimation is investigated analytically and numerically, revealing for instance the positive effect of a high signal-to-noise ratio and/or a large number of data points. As a fixed choice of the hyperparameter imposes certain results on the amplitude and frequency, this parameter is estimated and, thus, tested for plausibility as well. From abstract point of view, the model posterior is investigated as well.
文摘Objective: Computed tomography (CT)-based attenuation correction (CTAC) offers the clear benefit of reliable reconstruction of single-photon emission computed tomography (SPECT) images through its ability to achieve object-specific attenuation maps, but artifacts from dense materials often deteriorate CTAC performance. Therefore, we investigate the feasibility of CTAC in the presence of dense materials using dual-energy virtual monochromatic CT data. Methods: A sodium pertechnetate-filled cylindrical uniform phantom, with a pair of undiluted iodine syringes attached, is scanned with a dual-source CT scanner to obtain both single-energy (120 kVp) polychromatic and dual-energy (80 kVp/140 kVp with tin filtering) virtual monochromatic CT images. The single-energy and the dual-energy CT images are then converted to attenuation maps at 141 keV. SPECT images are reconstructed from 99mTc emission data of the phantom using each single-energy and dual-energy attenuation map and incorporating CTAC procedure. A region-of-in- terest analysis is performed to quantitatively compare the attenuation maps between the single-energy and the dual-energy techniques, each at an iodine-free position and a position adjacent to the iodine solutions. Results: At the iodine-free position, the phantom provides a uniform distribution of attenuation maps in both the single-energy and the dual-energy techniques. In the presence of adjacent iodine solutions, however, severe artifacts appeare in the single-energy CT images. These artifacts make attenuation values fluctuate, resulting in erroneous pixel values in the CTAC SPECT images. In contrast, dual-energy CT strongly suppresses the artifacts and hence improves the uniformity of the attenuation maps and the resultant SPECT images. Conclusions: Dual-energy CT with virtual monochromatic reconstruction has the potential to substantially reduce artifacts arising from dense materials. It has the potential to improve the accuracy of attenuation maps and the resultant CTAC SPECT images.
基金upported by the National Key R&D Plan of China(2016YFF0200802)Establishment of a standard device for air kerma in mammography X-rays(ANL1902)。
文摘Space scientific exploration is rapidly becoming the primary battlefield for humankind to explore the universe.Countries worldwide have launched numerous space exploration satellites.Accurate calibration of the detectors on the ground is a crucial element for space science satellites to obtain observational results.For the purpose of providing calibration for various satellite-borne detectors,multiple monochromatic X-rays facilities have been built at the National Institute of Metrology,P.R.China(NIM).These facilities mainly pertain to grating diffraction and Bragg diffraction,and the energy range of the produced monochromatic X-rays is 0.218–301 ke V.These facilities have a high performance in terms of energy stability,monochromaticity,and flux stability.The monochromaticity was greater than 3.0%.The energy stability of the facility is 0.02%at 25 ke V over 8 h,and the flux stability was within 1.0%at 25 ke V over 8 h.Calibration experiments on the properties of satellite-borne detectors,such as energy linearity,energy resolution,detection efficiency,and temperature response,can be conducted at the facilities.Thus far,the calibration of two satellites has been completed by the authors,and the work on three other satellites is in progress.This study will contribute to the advancement of X-ray astronomy the development of Chinese space science.
基金supported by the National Natural Science Foundation of China(Nos.61604028,11775203,and 12075219)the Chongqing Study Abroad Innovation Fund Project(No.cx2018023)+1 种基金the Plasma Physics Key Laboratory Fund Project(No.6142A04180207)the Innovation and Development Fund of China Academy of Engineering Physics(No.CX20210019)。
文摘In this study,a toroidal quartz(2023)crystal is designed for monochromatic X-ray imaging at 72.3◦.The designed crystal produces excellent images of a laser-produced plasma emitting He-like Ti X-rays at 4.75 keV.Based on the simulations,the imaging resolutions of the spherical and toroidal crystals in the sagittal direction are found to be 15 and 5μm,respectively.Moreover,the simulation results show that a higher resolution image of the source can be obtained by using a toroidal crystal.An X-ray backlight imaging experiment is conducted using 4.75 keV He-like Ti X-rays,a 3×3 metal grid,an imaging plate and a toroidal quartz crystal with a lattice constant of 2d=0.2749 nm.The meridional and sagittal radii of the toroidalα-quartz crystal are 295.6 and 268.5 mm,respectively.A highly resolved image of the microgrid,with a spatial resolution of 10μm,is obtained in the experiment.By using similar toroidal crystal designs,the application of a spatially resolved spectrometer with high-resolution X-ray imaging ability is capable of providing imaging data with the same magnification ratio in the sagittal and meridional planes.
基金supported by DOE/NLUF grant nos.DENA0000859 and DE-NA0002267,and LLNLsupported by the Technology Development Foundation of China Academy of Engineering Physics(2012A0401020)
文摘Temperature and density asymmetry diagnosis is critical to advance inertial confinement fusion(ICF) science. A multimonochromatic x-ray imager, MMI, records the spectral signature from an ICF implosion core with time resolution, 2D spatial resolution and spectral resolution. While narrow-band images and 2D space-resolved spectra from the MMI data constrain the temperature and the density spatial structure of the core, the accuracy of the images and the spectra highly depends on the quality of the MMI data and the processing tools. Here, we synthetically investigate the criterion for reliable MMI diagnostics and its effects on the accuracy of the reconstructed images. The pinhole array tilt determines the object spatial sampling efficiency and the minimum reconstruction width, w. When the spectral width associated with w is significantly narrower than the spectral linewidth, the line images reconstructed from the MMI data become reliable. The MMI setup has to be optimized for every application to meet this criterion for reliable ICF diagnostics.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.11625207 and 11972326)the Fundamental Research Funds for the Central Universities(Grant No.2652019071)。
文摘Measuring the temperature and deformation synchronously at elevated temperatures is technically challenging and has become a major concern in the evaluation of mechanical properties. In this study, a simple, easy-to-implement, yet effective monochromatic pyrometry is established for non-contact and full-field temperature measurements, which can significantly reduce the error caused by the camera’s channel crosstalk that commonly occurs in the existing improved two-color method. In addition, hightemperature digital image correlation, combined with band-pass filtering and monochromatic illumination, is applied for deformation measurement. Subsequently, an experimental system was set up to validate the accuracy of the proposed method,which consists of a CCD camera for image capturing, a blue bandpass filter for radiation suppression, blue light irradiation for light compensation, and an infrared pyrometer for temperature recording. The results of the thermal heating experiment on the C/SiC sample proved that the selection of camera channel R in monochromatic pyrometry can reduce the error by channel crosstalk,and the proposed method is applicable for synchronous measurement of temperature and deformation.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 69677020) and the Tianjin Foundation of Natural Science for this research.
文摘We report for the first time the theoretical analysis and experimental results of a white-light reconstructed monochromatic 3-D image synthesizing tomograms by multiple rainbow holo-graphy with vertical-area partition (VAP) approach. The theoretical and experimental results show that 3-D monochromatic image can be synthesized by recording the master hologram by VAP ap-proach without any distortions either in gray scale or in geometrical position. A 3-D monochromatic image synthesized from a series of medical tomograms is presented in this paper for the first time.
基金supported by the National Natural Science Foundation of China(Nos.11901196)Natural Science Foundation of Anhui Province(Nos.JZ2020AKZR0295)by the Scholarship Promotion Program of Hefei University of Technology(Nos.JZ2019HGTA0038)。
文摘A vertex-colored path P is rainbow if its internal vertices have distinct colors;whereas P is monochromatic if its internal vertices are colored the same.For a vertex-colored connected graph G,the rainbow vertex-connection number rvc(G)is the minimum number of colors used such that there is a rainbow path joining any two vertices of G;whereas the monochromatic vertex-connection number mvc(G)is the maximum number of colors used such that any two vertices of G are connected by a monochromatic path.These two opposite concepts are the vertex-versions of rainbow connection number rc(G)and monochromatic connection number mc(G)respectively.The study on rc(G)and mc(G)of random graphs drew much attention,and there are few results on the rainbow and monochromatic vertex-connection numbers.In this paper,we consider these two vertex-connection numbers of random graphs and establish sharp threshold functions for them,respectively.
基金supported by the National Key Research and Development Program(Grant No.2019YFE03080200)the National Natural Science Foundation of China(Grant No.12204353)。
文摘The measurement of X-ray continuous emission from laser-driven plasma was achieved through multiple monochromatic imaging utilizing a multilayer mirror array.This methodology was exemplified by the development of an eight-channel X-ray imaging system,capable of operating in the energy range of several ke V with a spatial resolution of approximately3 μm.By integrating this system with a streak camera,the temperature and trajectory of imploding capsules were successfully measured at the kJ-class Shenguang III prototype laser facility.This approach provides a synchronous diagnostic method for the spatial,temporal and spectral analysis of laser-driven plasma,characterized by its high efficiency and resolution.
文摘An application of the optical pyrometer is studied for measuring monochromatic emissivities of cement clinker with various Fe2O3 content.The idea of using “brightness temperature”is introduced into the emissivity measurement. In this method, there is no need for measuring an actual temperature of sample surfaces, only with determining both brightness temperatures of a sample and a blackbody can the required emissivity be evaluated according to Wien’s radiation law. In practice, the cement clinker is regarded as a greybody, the monochromatic emissivity is approximately equal to the total emissivity, so a single-colour optical pyrometer is applied for this purpose. Test measurements are carried out on 10 kinds of cement clinkers. Experimental data are treated by the least square method. As a result, the emissivity variation with temperature at a certain Fe2O3 content is quite well represented by εn=α+bT. Furthermore, this work first reported that the emissivities of cement clinker change considerably with Fe2O3 contents. In multiple cement production this conclusion is very important.
