With the support by the National Natural Science Foundation of China and Science and Technology Key Project of Shenzhen,the research team led by Prof.Yu Xuefeng(喻学锋)from Shenzhen Institutes of Advanced Technology,C...With the support by the National Natural Science Foundation of China and Science and Technology Key Project of Shenzhen,the research team led by Prof.Yu Xuefeng(喻学锋)from Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,cooperated with the team led by Prof.Paul K.展开更多
The cDNA sequence of Capal gene was cloned from Capsicum chinense Jacq by RT-PCR and sequenced. Bioinformatics analysis showed that Capal en- codes a putative polypeptide of 683 amino acids with a calculated molecular...The cDNA sequence of Capal gene was cloned from Capsicum chinense Jacq by RT-PCR and sequenced. Bioinformatics analysis showed that Capal en- codes a putative polypeptide of 683 amino acids with a calculated molecular mass of 74.2 kD and a theoretical pl of 6.9. Multiple sequence alignments and phyloge- netic tree analyses showed that Capal protein of C. chinense is similar to that of Capsicum annuum var. conoides, with an overall sequence similarity of 96%. The prokaryotic expression vector pET-32a-pal was constructed and induced to express in E. coil BL21. The SDS-PAGE analysis showed that the relative molecular mass of the induced new protein is about 74 kD, which was basically identical with that predicted by DNAMAN software （74.3 kD）, Real-time PCR analysis showed that ex- ogenous jasmonic acid （JA） promoted pal expression. The accumulation of capsaicin in pepper was analyzed by high performance liquid chromatography （HPLC）, and the results indicated that exogenous jasmonic acid （JA） can promote the synthesis of capsaicin. This study will provide valuable experimental basis for the research of transcription regulation and explaining the gene function of pal.展开更多
为了提高三相四开关并联型有源电力滤波器(active power filter,APF)的性能,提出一种基于比例积分-并联矢量比例积分(proportional-integral and vector proportional integral,PI-VPI)电流控制器的网侧电流直接控制方法。通过采用网侧...为了提高三相四开关并联型有源电力滤波器(active power filter,APF)的性能,提出一种基于比例积分-并联矢量比例积分(proportional-integral and vector proportional integral,PI-VPI)电流控制器的网侧电流直接控制方法。通过采用网侧电流直接控制方法,省去了对负载电流和APF输出电流的检测,不但可以减少电流传感器的使用,而且可以降低谐波电流检测精度对APF补偿效果的影响;并将PI-VPI控制引入到电压、电流的双闭环控制策略当中,以提高谐波补偿的精度。实验证明该方法可以将电网电流谐波总畸变率降至5.38%左右,而且具有较快的响应速度。展开更多
文摘With the support by the National Natural Science Foundation of China and Science and Technology Key Project of Shenzhen,the research team led by Prof.Yu Xuefeng(喻学锋)from Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,cooperated with the team led by Prof.Paul K.
基金Supported by Innovation Fund of Undergraduate Education in Jilin University(2012A82221)Jilin Provincial Natural Science and Technology Foundation(20101568)~~
文摘The cDNA sequence of Capal gene was cloned from Capsicum chinense Jacq by RT-PCR and sequenced. Bioinformatics analysis showed that Capal en- codes a putative polypeptide of 683 amino acids with a calculated molecular mass of 74.2 kD and a theoretical pl of 6.9. Multiple sequence alignments and phyloge- netic tree analyses showed that Capal protein of C. chinense is similar to that of Capsicum annuum var. conoides, with an overall sequence similarity of 96%. The prokaryotic expression vector pET-32a-pal was constructed and induced to express in E. coil BL21. The SDS-PAGE analysis showed that the relative molecular mass of the induced new protein is about 74 kD, which was basically identical with that predicted by DNAMAN software （74.3 kD）, Real-time PCR analysis showed that ex- ogenous jasmonic acid （JA） promoted pal expression. The accumulation of capsaicin in pepper was analyzed by high performance liquid chromatography （HPLC）, and the results indicated that exogenous jasmonic acid （JA） can promote the synthesis of capsaicin. This study will provide valuable experimental basis for the research of transcription regulation and explaining the gene function of pal.
文摘为了提高三相四开关并联型有源电力滤波器(active power filter,APF)的性能,提出一种基于比例积分-并联矢量比例积分(proportional-integral and vector proportional integral,PI-VPI)电流控制器的网侧电流直接控制方法。通过采用网侧电流直接控制方法,省去了对负载电流和APF输出电流的检测,不但可以减少电流传感器的使用,而且可以降低谐波电流检测精度对APF补偿效果的影响;并将PI-VPI控制引入到电压、电流的双闭环控制策略当中,以提高谐波补偿的精度。实验证明该方法可以将电网电流谐波总畸变率降至5.38%左右,而且具有较快的响应速度。
