Studies on the cold-responsive genes and cold signaling of woody species drop far behind in comparison to herbaceous plants.Due to similar lignified structure,perennial characteristic,and enhanced tolerance,it seems m...Studies on the cold-responsive genes and cold signaling of woody species drop far behind in comparison to herbaceous plants.Due to similar lignified structure,perennial characteristic,and enhanced tolerance,it seems much easier to find strongly antifreeze genes and obtain effective results in transgenic woody plants.In this study,Ammopiptanthus mongolicus,an evergreen,broadleaf and cold-resist leguminous shrub growing in the desert of Inner Mongolia,was used as a material for low-temperature induced gene isolation.Through differential expression analysis induced by low-temperature,thirteen up-regulated cDNAs were identified.One of them,AmEBP1,(accession number:DQ519359)confers enhanced cold-tolerance to both transgenic E.coli and transgenic Arabidopsis.Results suggest that AmEBP1 can stimulate the synthesis of ribosome and the dephosphyration of the α-subunit of initiation factor 2(eIF2α),and subsequently promote the translation process.By which the transgenic plants obtained increased cold-resistant ability.展开更多
A new species of the genus Tylototriton is described,from Yuexi county,Anhui province,in the south of the Dabie Mountains.It is based on morphological and molecular analysis.The new species is identified as belonging ...A new species of the genus Tylototriton is described,from Yuexi county,Anhui province,in the south of the Dabie Mountains.It is based on morphological and molecular analysis.The new species is identified as belonging to the Tylototriton asperrimus group and shares a number of similarities with T.wenxianensis,T.broadoridgus and T.dabienicus.The diagnostic characteristics of the new species are as follows： the head length is greater than the width of the head; bony ridges on the head are prominent and necked-in; the distal digit ends,ventral digits,peripheral area of the cloaca and the tail＇s lower edge are orange.The result from the molecular analysis of the genus Tylototriton（including the type specimen of the new species） based on three mitochondrial genes（ND1,ND2 and CYTB） indicated that the new species was close to T.wenxianensis,T.dabienicus,and T.broadoridgus,but formed an independent clade.This result was consistent with the morphological analysis above,which supports the theory that the population distributed in the south of the Dabie Mountains,namely in from Yuexi county,Anhui province,represented a distinct species,Tylototriton anhuiensis sp.nov.展开更多
Colorectal cancer(CRC) represents one of the most commonly diagnosed cancers worldwide.It is the second leading cause of cancer death in Western Countries.In the last decade the survival of patients with metastatic CR...Colorectal cancer(CRC) represents one of the most commonly diagnosed cancers worldwide.It is the second leading cause of cancer death in Western Countries.In the last decade the survival of patients with metastatic CRC has improved dramatically.Due to the advent of new drugs(irinotecan and oxaliplatin) and target therapies(i.e.,bevacizumab,cetuximab and panitumab),the median overall survival has risen from about 12 mo in the mid nineties to 30 mo recently.Many questions needing of right collocations and more clearness still exist regarding the prognostic factors and the predictive factors of response to therapy.Despite advances in dosing and scheduling of chemotherapy in both adjuvant and advanced settings,and a greater emphasis on early detection,the outlook still remains poor for most patients.Molecular analyses have shown that the natural history of all CRCs is not the same.Individual patients with same stage tumours may have different long term prognosis and response to therapy.In addition,some prognostic variables are likely to be more important than others.Here we review the role of prognostic factors and predictive factors according to the recently published English literature.展开更多
A new species of Macandrewia Gray from a seamount near Yap Trench in the Western Pacific Ocean was described.Macandrewia yapensis sp.nov.is distinct from its congeners by possessing a foliate shape with contorted lame...A new species of Macandrewia Gray from a seamount near Yap Trench in the Western Pacific Ocean was described.Macandrewia yapensis sp.nov.is distinct from its congeners by possessing a foliate shape with contorted lamellae,tuberculiform terminations of desmas,and unique size of spicules.This is the third species of Macandrewia described from the Pacific Ocean.In addition a partial sequence of COI gene was obtained from the new specimen and then it submitted to GenBank.Phylogenetic tree constructed with the partial COI sequences appears to exhibit a more congruent relationship with morphological data of macandrewiid species compared to 28 S gene tree.展开更多
A new unstriped Ichthyophis is found in Yunnan, China, it is distinguished from its unstriped congeners in morphology by a combination of characters including HL(head length), the number of annuli(369–372), vertebrae...A new unstriped Ichthyophis is found in Yunnan, China, it is distinguished from its unstriped congeners in morphology by a combination of characters including HL(head length), the number of annuli(369–372), vertebrae(114), inner mandibular teeth(36) and premaxillary-maxillary(51). The new species is also distinguished from its closest neighbour congener by a significant genetic divergence which is greater than 5.8% in the mitochondrial COI gene and greater than 6.3% in the mitochondrial cyt-b gene. As the first unstriped Ichthyophis species found in China,it is also the second species of Caecilians(Amphibian:Gymnophiona) found in China.展开更多
A new species of Leptobrachella is described from Sichuan Province and Chongqing Municipality,China.Molecular phylogenetic analyses based on mitochondrial and nuclear gene sequences indicated that the new species is g...A new species of Leptobrachella is described from Sichuan Province and Chongqing Municipality,China.Molecular phylogenetic analyses based on mitochondrial and nuclear gene sequences indicated that the new species is genetically divergent from its congeners.It could be identified from its congeners by a combination of followings characters:body size of male 29.1-34.1 mm(n=14),female 34.1-34.9 mm(n=4);dorsal skin rough with large tubercles in size of humeral glands,without conical spines;fringes on toes narrow;ventral body basically floral white with deep grey pigments all over;dorsal body deep greyish brown with smoky black markings;iris gold above,gradually silver bellow;tibia-tarsal articulation reaches the level of the middle of the eye when leg being stretched forward;the main call type with dominant frequency4.08 ± 0.16 kHz(14.1-14.9℃),call duration 170.35± 15.19ms,the number of pulses for the first note in a call 3.50±0.89,and the number of pulses for the second note in a call 5.08±0.77.展开更多
A new species of the genus Liurana is described from Medog,Xizang,China,based on morphological and molecular data.Molecular phylogenetic analysis based on the mitochondrial gene COI indicated that this new species rep...A new species of the genus Liurana is described from Medog,Xizang,China,based on morphological and molecular data.Molecular phylogenetic analysis based on the mitochondrial gene COI indicated that this new species represented an independent evolutionary lineage.In addition,the uncorrected genetic distance between the new species and its closest congener species,L.alpina,was 9.6%-9.8%for COI.The new species,Liurana namchabarwa sp.nov.Yu,Lin,Wang,Jiang and Xie,could be distinguished from its congeners by following characters:(1)relatively large body size in this genus,SVL=21.2-23.1 mm in adult males(n=3);(2)relatively narrow head(HL/HW=100.5%-104.8%(n=3));(3)digital disc not expanded;(4)relatively long hindlimbs,with tibiotarsal articulation reaching tip of snout when adpressed.展开更多
To examine the eukaryotic biodiversity of aquatic ecosystems in the Qiantang River,China,eukaryotic microbes in the river were investigated using 18S rRNA gene sequencing during the breeding season(July to August 2018...To examine the eukaryotic biodiversity of aquatic ecosystems in the Qiantang River,China,eukaryotic microbes in the river were investigated using 18S rRNA gene sequencing during the breeding season(July to August 2018).Four distinct distribution patterns(1.Jiande;2.Tonglu and Fuyang;3.Jiubao;4.Yanguan)of the microbial community and their potential effects on fishery activities were observed.Results show lower abundances of Dinophyta and Fungi and higher abundances of Cryptophyta and Chlorophyta in Tonglu and Fuyang than those in the other three sections.In addition,the reserves(Tonglu and Fuyang)destabilized the original eukaryotic microbial co-occurrence network.Among all the environmental factors measured,nitrogen(nitrite,nitrate,ammonium),water temperature and total chlorophyll a acted as major driving factors that controlled the eukaryotic microbial distribution.Furthermore,the existence of some algae(e.g.,Chrysophyceae,Cryptophytes,and Chlorophyceae)and fungi(e.g.,Rhizophydium)in Tonglu and Fuyang was beneficial to juvenile fish growth and water quality,although some detrimental species(e.g.,Aphanomyces)needed attention.This study provides further insights into the sustainable protection and utilization of rivers.展开更多
Combined Hepatocellular-Cholangiocarcinoma is a heterogenous primary malignant epithelial tumor of the liver with variable morphological and immunophenotypical features.Although the biology of this tumor has been desc...Combined Hepatocellular-Cholangiocarcinoma is a heterogenous primary malignant epithelial tumor of the liver with variable morphological and immunophenotypical features.Although the biology of this tumor has been described in the literature,changes in classification and its heterogeneity imply difficulties in collecting reliable homogenous groups to compare.The article aims to review available data on morphology and immunohistochemistry for practicing pathologists integrated with original data from our referral Center.展开更多
The escalating use of nanodiamonds(NDs)has raised concerns about their ecotoxicological impact,prompting exploration of therapeutic interventions.This paper pioneers the examination of Vitamin B12-conjugated sericin(V...The escalating use of nanodiamonds(NDs)has raised concerns about their ecotoxicological impact,prompting exploration of therapeutic interventions.This paper pioneers the examination of Vitamin B12-conjugated sericin(VB12-SER)as a potential therapeutic approach against ND-induced toxicity in darkling beetles(Blaps polychresta).The study analyzes mortality rates and organ-specific effects,covering the testis,ovary,and midgut,before and after treatments.Following exposure to 10 mg NDs/g body weight,within a subgroup of individuals termed ND2 with a mortality rate below 50%,two therapeutic treatments were administered,including pure sericin(SER)at 10 mg/mL and VB12-SER at 10.12 mg/mL.Consequently,five experimental groups(control,SER,ND2,ND2+SER,ND2+SER+VB12)were considered.Kaplan–Meier survival analysis was performed to assess the lifespan distribution of the insects in these groups over a 30-d period.Analyses revealed increased mortality and significant abnormalities induced by NDs within the examined organs,including cell death,DNA damage,enzyme dysregulation,antioxidant imbalances,protein depletion,lipid peroxidation,and morphological deformities.In contrast,the proposed treatments,especially(ND2+SER+VB12),demonstrated remarkable recovery,highlighting VB12-conjugated SER's potential in mitigating ND-triggered adverse effects.Molecular docking simulations affirmed binding stability and favorable interactions of the VB12-SER complex with target proteins.This research enhances understanding of NDs’effects on B.polychresta,proposing it as an effective bioindicator,and introduces VB12-conjugated SER as a promising therapeutic strategy in nanotoxicological studies.展开更多
文摘Studies on the cold-responsive genes and cold signaling of woody species drop far behind in comparison to herbaceous plants.Due to similar lignified structure,perennial characteristic,and enhanced tolerance,it seems much easier to find strongly antifreeze genes and obtain effective results in transgenic woody plants.In this study,Ammopiptanthus mongolicus,an evergreen,broadleaf and cold-resist leguminous shrub growing in the desert of Inner Mongolia,was used as a material for low-temperature induced gene isolation.Through differential expression analysis induced by low-temperature,thirteen up-regulated cDNAs were identified.One of them,AmEBP1,(accession number:DQ519359)confers enhanced cold-tolerance to both transgenic E.coli and transgenic Arabidopsis.Results suggest that AmEBP1 can stimulate the synthesis of ribosome and the dephosphyration of the α-subunit of initiation factor 2(eIF2α),and subsequently promote the translation process.By which the transgenic plants obtained increased cold-resistant ability.
基金partly supported by the Graduate Student Academic Innovation Research Project of Anhui University(yqh100101)the National Key Research and Development Programme(2016YFC1200700)
文摘A new species of the genus Tylototriton is described,from Yuexi county,Anhui province,in the south of the Dabie Mountains.It is based on morphological and molecular analysis.The new species is identified as belonging to the Tylototriton asperrimus group and shares a number of similarities with T.wenxianensis,T.broadoridgus and T.dabienicus.The diagnostic characteristics of the new species are as follows： the head length is greater than the width of the head; bony ridges on the head are prominent and necked-in; the distal digit ends,ventral digits,peripheral area of the cloaca and the tail＇s lower edge are orange.The result from the molecular analysis of the genus Tylototriton（including the type specimen of the new species） based on three mitochondrial genes（ND1,ND2 and CYTB） indicated that the new species was close to T.wenxianensis,T.dabienicus,and T.broadoridgus,but formed an independent clade.This result was consistent with the morphological analysis above,which supports the theory that the population distributed in the south of the Dabie Mountains,namely in from Yuexi county,Anhui province,represented a distinct species,Tylototriton anhuiensis sp.nov.
文摘Colorectal cancer(CRC) represents one of the most commonly diagnosed cancers worldwide.It is the second leading cause of cancer death in Western Countries.In the last decade the survival of patients with metastatic CRC has improved dramatically.Due to the advent of new drugs(irinotecan and oxaliplatin) and target therapies(i.e.,bevacizumab,cetuximab and panitumab),the median overall survival has risen from about 12 mo in the mid nineties to 30 mo recently.Many questions needing of right collocations and more clearness still exist regarding the prognostic factors and the predictive factors of response to therapy.Despite advances in dosing and scheduling of chemotherapy in both adjuvant and advanced settings,and a greater emphasis on early detection,the outlook still remains poor for most patients.Molecular analyses have shown that the natural history of all CRCs is not the same.Individual patients with same stage tumours may have different long term prognosis and response to therapy.In addition,some prognostic variables are likely to be more important than others.Here we review the role of prognostic factors and predictive factors according to the recently published English literature.
基金the Science&Technology Basic Resources Investigation Program of China(No.2017FY100804)the Biological Resources Program,Chinese Academy of Sciences(No.KFJ-BRP-017-37)+1 种基金the National Key R&D Program of China(No.2018YFC0309804)the National Natural Science Foundation of China(No.41706188)。
文摘A new species of Macandrewia Gray from a seamount near Yap Trench in the Western Pacific Ocean was described.Macandrewia yapensis sp.nov.is distinct from its congeners by possessing a foliate shape with contorted lamellae,tuberculiform terminations of desmas,and unique size of spicules.This is the third species of Macandrewia described from the Pacific Ocean.In addition a partial sequence of COI gene was obtained from the new specimen and then it submitted to GenBank.Phylogenetic tree constructed with the partial COI sequences appears to exhibit a more congruent relationship with morphological data of macandrewiid species compared to 28 S gene tree.
文摘A new unstriped Ichthyophis is found in Yunnan, China, it is distinguished from its unstriped congeners in morphology by a combination of characters including HL(head length), the number of annuli(369–372), vertebrae(114), inner mandibular teeth(36) and premaxillary-maxillary(51). The new species is also distinguished from its closest neighbour congener by a significant genetic divergence which is greater than 5.8% in the mitochondrial COI gene and greater than 6.3% in the mitochondrial cyt-b gene. As the first unstriped Ichthyophis species found in China,it is also the second species of Caecilians(Amphibian:Gymnophiona) found in China.
基金supported by West Light Foundation of The Chinese Academy of Sciences(Grant No.2021XBZG_XBQNXZ_A_006)National Natural Sciences Foundation of China(Grant Nos.:32270498 and 32070426)China Biodiversity Observation Networks(Sino BON-Amphibian and Reptile)。
文摘A new species of Leptobrachella is described from Sichuan Province and Chongqing Municipality,China.Molecular phylogenetic analyses based on mitochondrial and nuclear gene sequences indicated that the new species is genetically divergent from its congeners.It could be identified from its congeners by a combination of followings characters:body size of male 29.1-34.1 mm(n=14),female 34.1-34.9 mm(n=4);dorsal skin rough with large tubercles in size of humeral glands,without conical spines;fringes on toes narrow;ventral body basically floral white with deep grey pigments all over;dorsal body deep greyish brown with smoky black markings;iris gold above,gradually silver bellow;tibia-tarsal articulation reaches the level of the middle of the eye when leg being stretched forward;the main call type with dominant frequency4.08 ± 0.16 kHz(14.1-14.9℃),call duration 170.35± 15.19ms,the number of pulses for the first note in a call 3.50±0.89,and the number of pulses for the second note in a call 5.08±0.77.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research Program(2019QZKK05010203)the Survey of Wildlife Resources in Key Areas of Xizang(Phase II,ZL202303601)Program of conservation measures study on amphibians and reptiles of Grand Canyon National Nature Reserve,and China Biodiversity Observation Networks(Sino BON).
文摘A new species of the genus Liurana is described from Medog,Xizang,China,based on morphological and molecular data.Molecular phylogenetic analysis based on the mitochondrial gene COI indicated that this new species represented an independent evolutionary lineage.In addition,the uncorrected genetic distance between the new species and its closest congener species,L.alpina,was 9.6%-9.8%for COI.The new species,Liurana namchabarwa sp.nov.Yu,Lin,Wang,Jiang and Xie,could be distinguished from its congeners by following characters:(1)relatively large body size in this genus,SVL=21.2-23.1 mm in adult males(n=3);(2)relatively narrow head(HL/HW=100.5%-104.8%(n=3));(3)digital disc not expanded;(4)relatively long hindlimbs,with tibiotarsal articulation reaching tip of snout when adpressed.
基金Supported by the Fisheries Species Conservation Program of the Agricultural Department of China(Nos.171821303154051044,17190236)the Natural Science Foundation of Zhejiang Province(No.LQ20C190003)+1 种基金the Natural Science Foundation of Ningbo Municipality(Nos.2019A610421,2019A610443)the K.C.Wong Magna Fund of Ningbo University。
文摘To examine the eukaryotic biodiversity of aquatic ecosystems in the Qiantang River,China,eukaryotic microbes in the river were investigated using 18S rRNA gene sequencing during the breeding season(July to August 2018).Four distinct distribution patterns(1.Jiande;2.Tonglu and Fuyang;3.Jiubao;4.Yanguan)of the microbial community and their potential effects on fishery activities were observed.Results show lower abundances of Dinophyta and Fungi and higher abundances of Cryptophyta and Chlorophyta in Tonglu and Fuyang than those in the other three sections.In addition,the reserves(Tonglu and Fuyang)destabilized the original eukaryotic microbial co-occurrence network.Among all the environmental factors measured,nitrogen(nitrite,nitrate,ammonium),water temperature and total chlorophyll a acted as major driving factors that controlled the eukaryotic microbial distribution.Furthermore,the existence of some algae(e.g.,Chrysophyceae,Cryptophytes,and Chlorophyceae)and fungi(e.g.,Rhizophydium)in Tonglu and Fuyang was beneficial to juvenile fish growth and water quality,although some detrimental species(e.g.,Aphanomyces)needed attention.This study provides further insights into the sustainable protection and utilization of rivers.
文摘Combined Hepatocellular-Cholangiocarcinoma is a heterogenous primary malignant epithelial tumor of the liver with variable morphological and immunophenotypical features.Although the biology of this tumor has been described in the literature,changes in classification and its heterogeneity imply difficulties in collecting reliable homogenous groups to compare.The article aims to review available data on morphology and immunohistochemistry for practicing pathologists integrated with original data from our referral Center.
文摘The escalating use of nanodiamonds(NDs)has raised concerns about their ecotoxicological impact,prompting exploration of therapeutic interventions.This paper pioneers the examination of Vitamin B12-conjugated sericin(VB12-SER)as a potential therapeutic approach against ND-induced toxicity in darkling beetles(Blaps polychresta).The study analyzes mortality rates and organ-specific effects,covering the testis,ovary,and midgut,before and after treatments.Following exposure to 10 mg NDs/g body weight,within a subgroup of individuals termed ND2 with a mortality rate below 50%,two therapeutic treatments were administered,including pure sericin(SER)at 10 mg/mL and VB12-SER at 10.12 mg/mL.Consequently,five experimental groups(control,SER,ND2,ND2+SER,ND2+SER+VB12)were considered.Kaplan–Meier survival analysis was performed to assess the lifespan distribution of the insects in these groups over a 30-d period.Analyses revealed increased mortality and significant abnormalities induced by NDs within the examined organs,including cell death,DNA damage,enzyme dysregulation,antioxidant imbalances,protein depletion,lipid peroxidation,and morphological deformities.In contrast,the proposed treatments,especially(ND2+SER+VB12),demonstrated remarkable recovery,highlighting VB12-conjugated SER's potential in mitigating ND-triggered adverse effects.Molecular docking simulations affirmed binding stability and favorable interactions of the VB12-SER complex with target proteins.This research enhances understanding of NDs’effects on B.polychresta,proposing it as an effective bioindicator,and introduces VB12-conjugated SER as a promising therapeutic strategy in nanotoxicological studies.
