The neuroinflammatory response mediated by microglial activation plays an important role in the secondary nerve injury of traumatic brain injury.The post-transcriptional modification of N^(6)-methyladenosine is ubiqui...The neuroinflammatory response mediated by microglial activation plays an important role in the secondary nerve injury of traumatic brain injury.The post-transcriptional modification of N^(6)-methyladenosine is ubiquitous in the immune response of the central nervous system.The fat mass and obesity-related protein catalyzes the demethylation of N^(6)-methyladenosine modifications on mRNA and is widely expressed in various tissues,participating in the regulation of multiple diseases’biological processes.However,the role of fat mass and obesity in microglial activation and the subsequent neuroinflammatory response after traumatic brain injury is unclear.In this study,we found that the expression of fat mass and obesity was significantly down-regulated in both lipopolysaccharide-treated BV2 cells and a traumatic brain injury mouse model.After fat mass and obesity interference,BV2 cells exhibited a pro-inflammatory phenotype as shown by the increased proportion of CD11b^(+)/CD86^(+)cells and the secretion of pro-inflammatory cytokines.Fat mass and obesity-mediated N^(6)-methyladenosine demethylation accelerated the degradation of ADAM17 mRNA,while silencing of fat mass and obesity enhanced the stability of ADAM17 mRNA.Therefore,down-regulation of fat mass and obesity expression leads to the abnormally high expression of ADAM17 in microglia.These results indicate that the activation of microglia and neuroinflammatory response regulated by fat mass and obesity-related N^(6)-methyladenosine modification plays an important role in the pro-inflammatory process of secondary injury following traumatic brain injury.展开更多
In recent years,exosomes have garnered extensive attention as therapeutic agents and early diagnostic markers in neurodegenerative disease research.Exosomes are small and can effectively cross the blood-brain barrier,...In recent years,exosomes have garnered extensive attention as therapeutic agents and early diagnostic markers in neurodegenerative disease research.Exosomes are small and can effectively cross the blood-brain barrier,allowing them to target deep brain lesions.Recent studies have demonstrated that exosomes derived from different cell types may exert therapeutic effects by regulating the expression of various inflammatory cytokines,mRNAs,and disease-related proteins,thereby halting the progression of neurodegenerative diseases and exhibiting beneficial effects.However,exosomes are composed of lipid bilayer membranes and lack the ability to recognize specific target cells.This limitation can lead to side effects and toxicity when they interact with non-specific cells.Growing evidence suggests that surface-modified exosomes have enhanced targeting capabilities and can be used as targeted drug-delivery vehicles that show promising results in the treatment of neurodegenerative diseases.In this review,we provide an up-to-date overview of existing research aimed at devising approaches to modify exosomes and elucidating their therapeutic potential in neurodegenerative diseases.Our findings indicate that exosomes can efficiently cross the blood-brain barrier to facilitate drug delivery and can also serve as early diagnostic markers for neurodegenerative diseases.We introduce the strategies being used to enhance exosome targeting,including genetic engineering,chemical modifications(both covalent,such as click chemistry and metabolic engineering,and non-covalent,such as polyvalent electrostatic and hydrophobic interactions,ligand-receptor binding,aptamer-based modifications,and the incorporation of CP05-anchored peptides),and nanomaterial modifications.Research into these strategies has confirmed that exosomes have significant therapeutic potential for neurodegenerative diseases.However,several challenges remain in the clinical application of exosomes.Improvements are needed in preparation,characterization,and optimization methods,as well as in reducing the adverse reactions associated with their use.Additionally,the range of applications and the safety of exosomes require further research and evaluation.展开更多
Research into lactylation modifications across various target organs in both health and disease has gained significant attention.Many essential life processes and the onset of diseases are not only related to protein ...Research into lactylation modifications across various target organs in both health and disease has gained significant attention.Many essential life processes and the onset of diseases are not only related to protein abundance but are also primarily regulated by various post-translational protein modifications.Lactate,once considered merely a byproduct of anaerobic metabolism,has emerged as a crucial energy substrate and signaling molecule involved in both physiological and pathological processes within the nervous system.Furthermore,recent studies have emphasized the significant role of lactate in numerous neurological diseases,including Alzheimer's disease,Parkinson's disease,acute cerebral ischemic stroke,multiple sclerosis,Huntington's disease,and myasthenia gravis.The purpose of this review is to synthesize the current research on lactate and lactylation modifications in neurological diseases,aiming to clarify their mechanisms of action and identify potential therapeutic targets.As such,this work provides an overview of the metabolic regulatory roles of lactate in various disorders,emphasizing its involvement in the regulation of brain function.Additionally,the specific mechanisms of brain lactate metabolism are discussed,suggesting the unique roles of lactate in modulating brain function.As a critical aspect of lactate function,lactylation modifications,including both histone and non-histone lactylation,are explored,with an emphasis on recent advancements in identifying the key regulatory enzymes of such modifications,such as lactylation writers and erasers.The effects and specific mechanisms of abnormal lactate metabolism in diverse neurological diseases are summarized,revealing that lactate acts as a signaling molecule in the regulation of brain functions and that abnormal lactate metabolism is implicated in the progression of various neurological disorders.Future research should focus on further elucidating the molecular mechanisms underlying lactate and lactylation modifications and exploring their potential as therapeutic targets for neurological diseases.展开更多
Post-translational modifications(PTMs)regulate the occurrence and development of cancer,and lactylation modification is a new form of PTMs.Recent studies have found that lactic acid modification can regulate the immun...Post-translational modifications(PTMs)regulate the occurrence and development of cancer,and lactylation modification is a new form of PTMs.Recent studies have found that lactic acid modification can regulate the immune tolerance of cancer cells.The classical theory holds that prostate apoptosis response-4(PAR-4)is a tumor suppressor protein.However,our recent research has found that PAR-4 has a biological function of promoting cancer in hepatocellular carcinoma(HCC),and our analysis shows that PAR-4 can be modified of lactic acid.These research evidences suggest that PAR-4 lactylation modification may drive immune tolerance in HCC.Therefore,inhibiting PAR-4 lactylation modification is very likely to increase the sensitivity of HCC to immunotherapy.展开更多
Aberrant RNA modification has been linked to the pathogenesis of various diseases;however,its specific molecular mechanisms in spinal cord injury remain poorly understood.The objective of this study was to explore RNA...Aberrant RNA modification has been linked to the pathogenesis of various diseases;however,its specific molecular mechanisms in spinal cord injury remain poorly understood.The objective of this study was to explore RNA modification-related biomarkers of spinal cord injury.The mRNA expression profiles of mice with spinal cord injury were retrieved from the Gene Expression Omnibus(GEO)database(GSE18179).We identified 185 differentially expressed genes using bioinformatics approaches.Functional enrichment analysis demonstrated aberrant activation or inhibition of common metabolism-related pathways,including sulfur metabolism and steroid biosynthesis,in mice with spinal cord injury.An integrated strategy comprising weighted gene co-expression network analysis,a random forest model,a support vector machine model,and a generalized linear model was employed to identify four genes whose aberrant RNA modification was linked to spinal cord injury:Elovl6,Idi1,Sqle,and Stbd1.We verified the expression levels and diagnostic performance of these four genes in the original training dataset and mouse samples via receiver operating characteristic curve analysis.Quantitative reverse transcription-polymerase chain reaction demonstrated variations in the mRNA levels of the four genes between the Sham and spinal cord injury groups at different time points following injury.We also constructed microRNA-mRNA and transcription factor-mRNA interaction networks using Cytoscape.Additionally,we evaluated the proportions of 22 types of immune cells in the spinal cords of mice using the CIBERSORT tool,revealing significant alterations in the numbers of memory B cells,resting dendritic cells,M0 macrophages,activated mast cells,resting mast cells,and CD8+T cells in spinal cord injury mice compared with Sham controls.Microglia and T cells were identified as key cell types by single-cell sequencing analysis.These findings provide new directions for the development of RNA modification-related therapeutic strategies for spinal cord injury and suggest that Elovl6,Idi1,Sqle,and Stbd1 are potential biomarkers of spinal cord injury.展开更多
Real time prosody modification involves changing the prosody parameters such as pitch, duration and intensity of speech in real time without affecting the intelligibility and naturalness. In this paper prosody modific...Real time prosody modification involves changing the prosody parameters such as pitch, duration and intensity of speech in real time without affecting the intelligibility and naturalness. In this paper prosody modification is performed using instants of significant excitation (ISE) of the vocal tract system during production of speech. In the conventional prosody modification system the ISE are computed using group delay function, and it is computationally intensive task. In this paper, we propose computationally efficient methods to determine the ISE suitable for prosody modification in interactive (real time) applications. The overall computational time for the prosody modification by using the proposed method is compared with the conventional prosody modification method which uses the group delay function for computing the ISE.展开更多
We are sorry for the mistakes of Affiliation,"a State Key Laboratory of Advanced Fiber Materials,Center for Advanced Low-Dimension Materials,Donghua University,Shanghai 201620,China"should be replaced by&quo...We are sorry for the mistakes of Affiliation,"a State Key Laboratory of Advanced Fiber Materials,Center for Advanced Low-Dimension Materials,Donghua University,Shanghai 201620,China"should be replaced by"a State Key Laboratory of Advanced Fiber Materials,Center for Advanced Low-Dimension Materials,College of Materials Science and Engineering,Donghua University,Shanghai 201620,China".We apologized for the inconvenience caused by this error.展开更多
Before-and-after methods have been effectively used in the road safety studies to estimate Crash Modification Factors (CMFs) of individual treatments as well as the multiple treatments on roadways. Since the common pr...Before-and-after methods have been effectively used in the road safety studies to estimate Crash Modification Factors (CMFs) of individual treatments as well as the multiple treatments on roadways. Since the common practice is to apply multiple treatments on road segments, it is important to have a method to estimate CMFs of individual treatment so that the effect of each treatment towards improving the road safety can be identified. Even though there are methods introduced by researchers to combine multiple CMFs or to isolate the safety effectiveness of individual treatment from CMFs developed for multiple treatments, those methods have to be tested before using them. This study considered two multiple treatments namely 1) Safety edge with lane widening 2) Adding 2 ft paved shoulders with shoulder rumble strips and/or asphalt resurfacing. The objectives of this research are to propose a regression-based method to estimate individual CMFs estimate CMFs using before-and-after Empirical Bayes method and compare the results. The results showed that having large sample size gives accurate predictions with smaller standard error and p-values of the considered treatments. Also, results obtained from regression method are similar to the EB method even though the values are not exactly the same. Finally, it was seen that the safety edge treatment reduces crashes by 15% - 25% and adding 2 ft shoulders with rumble strips reduces crashes by 25% - 49%.展开更多
Spinal cord injury typically causes corticospinal tract disruption.Although the disrupted corticospinal tract can self-regenerate to a certain degree,the underlying mechanism of this process is still unclear.N6-methyl...Spinal cord injury typically causes corticospinal tract disruption.Although the disrupted corticospinal tract can self-regenerate to a certain degree,the underlying mechanism of this process is still unclear.N6-methyladenosine(m^(6)A)modifications are the most common form of epigenetic regulation at the RNA level and play an essential role in biological processes.However,whether m^(6)A modifications participate in corticospinal tract regeneration after spinal cord injury remains unknown.We found that expression of methyltransferase 14 protein(METTL14)in the locomotor cortex was high after spinal cord injury and accompanied by elevated m^(6)A levels.Knockdown of Mettl14 in the locomotor cortex was not favorable for corticospinal tract regeneration and neurological recovery after spinal cord injury.Through bioinformatics analysis and methylated RNA immunoprecipitation-quantitative polymerase chain reaction,we found that METTL14 regulated Trib2 expression in an m^(6)A-regulated manner,thereby activating the mitogen-activated protein kinase pathway and promoting corticospinal tract regeneration.Finally,we administered syringin,a stabilizer of METTL14,using molecular docking.Results confirmed that syringin can promote corticospinal tract regeneration and facilitate neurological recovery by stabilizing METTL14.Findings from this study reveal that m^(6)A modification is involved in the regulation of corticospinal tract regeneration after spinal cord injury.展开更多
Viruses and their hosts have co-evolved for million years. In order to successfully replicate their genome, viruses need to usurp the biosynthetic machinery of the host cell. Depending on the complexity and the nature...Viruses and their hosts have co-evolved for million years. In order to successfully replicate their genome, viruses need to usurp the biosynthetic machinery of the host cell. Depending on the complexity and the nature of the genome, replication might involve or not a relatively large subset of viral products, in addition to a number of host cell factors, and take place in several subcellular compartments, including the nucleus,the cytoplasm, as well as virus-induced, rearranged membranes. Therefore viruses need to ensure the correct subcellular localization of their effectors and to be capable of disguising from the cellular defensive mechanisms. In addition, viruses are capable of exploiting host cell activities, by modulating their post-translational modification apparatus, resulting in profound modifications in the function of cellular and viral products. Not surprisingly infection of host cells by these parasites can lead to alterations of cellular differentiation and growing properties, with important pathogenic consequences. In the present hot topic highlight entitled "Reprogramming the host: modification of cell functions upon viral infection", a number of leading virologists and cell biologist thoroughly describe recent advances in our understanding of how viruses modulate cellular functions to achieve successful replication and propagation at the expenses of human cells.展开更多
As one of the most promising new energy sources,hydrogen energy is expected to usher in a full-fledged“hydrogen economy”in the 21st century.Compared with traditional high-pressure gaseous and cryogenic liquid hydrog...As one of the most promising new energy sources,hydrogen energy is expected to usher in a full-fledged“hydrogen economy”in the 21st century.Compared with traditional high-pressure gaseous and cryogenic liquid hydrogen storage methods,solid-state chemical hydrogen storage shows significant advantages in safety,high efficiency,and cost-effectiveness.Magnesium-based lightweight hydrogen storage materials have attracted widespread attention due to their high gravimetric hydrogen storage density(7.6%)and favorable reversibility.However,their sluggish reaction kinetics and stringent operating conditions(with H2 release temperatures exceeding 350°C and H2 absorption pressures above 4 MPa)pose major challenges for practical applications.Domestic and international researchers have conducted in-depth studies to address these issues,achieving substantial progress in the modification of magnesium-based hydrogen storage alloys.This paper systematically elaborates on major modification techniques such as alloying,nanostructuring,and catalytic material doping,providing a comprehensive analysis of the strengths and limitations of each approach.Furthermore,it offers prospects for the future development of magnesium-based hydrogen storage materials by integrating current theoretical and experimental research findings.展开更多
Biomass-derived carbon materials are favored for their abundance and sustainability,and ease of preparation and modification.By surface activation and modification they can have a good electrical conductivity,excellen...Biomass-derived carbon materials are favored for their abundance and sustainability,and ease of preparation and modification.By surface activation and modification they can have a good electrical conductivity,excellent catalytic activity,a remarkable adsorption capacity,and different interfacial physicochemical functionalities.Surface-modified biochars have found wide applications in energy storage,environmental remediation,and catalysis.However,achieving precise and controllable modification of their active sites remains a challenge.Recent advances and future prospects for controlling their surface morphology,defect engineering,and surface coating strategies,with particular attention to their means of fabrication,are reviewed.展开更多
Bentonite is a necessary binder in producing pellets.Its excessive use reduces the iron grade of pellets and increases production costs.Minimizing bentonite dosage is essential for producing high-quality iron ore pell...Bentonite is a necessary binder in producing pellets.Its excessive use reduces the iron grade of pellets and increases production costs.Minimizing bentonite dosage is essential for producing high-quality iron ore pellets.Addressing the gap in the application of organically-intercalated modified bentonite in the pelletizing field,this study introduces an innovative modification process for bentonite that employs the synergistic effect of mechanical force and dimethyl sulfoxide to enhance the intercalation of organic compounds within bentonite,thus significantly enhancing its binding performance.The colloid value and swell capacity of modified bentonite(98.5 m L/3g and 55.0 m L/g)were much higher than the original bentonite(90.5 m L/3g and 17.5 m L/g).With the decrease of bentonite dosage from1.5wt%to 1.0wt%,the drop number of green pellets from a height of 0.5 m and the compressive strengths of roasted pellets using the modified bentonite(6.0 times and 2916 N per pellet)were significantly higher than those of the original bentonite(4.0 times and 2739 N per pellet).This study provides a comprehensive analysis of the intercalation modification mechanism of bentonite,offering crucial technical insights for the development of high-performance modified bentonite as iron ore pellet binders.展开更多
Two-step-processed(TSP)inverted p-i-n perovskite solar cells(PSCs)have demonstrated significant promise in tandem applications.However,the power conversion efficiency(PCE)of TSP p-i-n PSCs rarely exceeds 24%.Here,we d...Two-step-processed(TSP)inverted p-i-n perovskite solar cells(PSCs)have demonstrated significant promise in tandem applications.However,the power conversion efficiency(PCE)of TSP p-i-n PSCs rarely exceeds 24%.Here,we demonstrate that TSP perovskite films exhibit a vertically gradient distribution of residual PbI_(2)clusters,which form Schottky heterojunctions with the perovskite,leading to substantial interfacial energy-level mismatches within NiO_(x)-based TSP p-i-n PSCs.These limitations were effectively addressed via a vertical interfacial engineering enabled by dual-interface modification incorporating tin trifluoromethanesulfonate(Sn(OTF)_(2))and 4-Fluorophenylethylamine chloride(F-PEA)at the NiO_(x)/perovskite and perovskite/C60 interfaces,respectively.The functional Sn(OTF)_(2)not only enhances the conductivity of NiO_(x)films but also suppresses ion migration,while inducing the formation of a Pb-Sn mixed perovskite interlayer that precisely regulates the energy level at the NiO_(x)/perovskite interface.Complementally,F-PEA post-treatment effectively converts surface residual PbI_(2)clusters into a 2D perovskite capping layer,which simultaneously passivates surface defects and enhances energy-level alignment at the perovskite/C60 interface.Consequently,the optimized NiO_(x)-based TSP p-i-n PSCs achieve a notable PCE of 25.6%with superior operational stability.This study elucidates the underlying mechanisms limiting the efficiency of TSP p-i-n PSCs,while establishing design principles for these devices targeting 26%efficiency.展开更多
The presence of a surface oxide film(B_(2)O_(3))on boron(B)particles significantly compromises their combustion efficiency and kinetic performance in fuel-rich solid propellants.This study proposes an innovative conti...The presence of a surface oxide film(B_(2)O_(3))on boron(B)particles significantly compromises their combustion efficiency and kinetic performance in fuel-rich solid propellants.This study proposes an innovative continuous modification strategy combining non-thermal plasma(NTP)etching with fluorocarbon passivation.Characterization and kinetic analysis revealed that reactive plasma species—including atomic hydrogen(H),electronically excited molecular hydrogen(H_(2)^(*)),vibrationally excited molecular hydrogen(H_(2)v),and hydrogen ions(H^(+))—dominate the reduction of B_(2)O_(3)through lowering the transition energy barrier and shifting the reaction spontaneity.Subsequent argon plasma fragmentation of C_(8)F_(18)generates fluorocarbon radicals that form conformal passivation coatings(thickness:7 nm)on purified boron surfaces.The modified boron particles exhibit 37.5℃lower exothermic peak temperature and 27.2%higher heat release(14.8 kJ/g vs.11.6 kJ/g)compared to untreated counterparts.Combustion diagnostics reveal 194%increase in maximum flame height(135.10 mm vs.46.03 mm)and 134%enhancement in flame propagation rate(4.44 cm/s vs.1.90 cm/s).This NTP-based surface engineering approach establishes a scalable pathway for developing highperformance boron-based energetic composites.展开更多
In order to eliminate the meshing interference between the flexspline and circular spline after the taper deformation of the flexspline,the radial deformation difference method,major and minor axis fitting method,and ...In order to eliminate the meshing interference between the flexspline and circular spline after the taper deformation of the flexspline,the radial deformation difference method,major and minor axis fitting method,and ellipse fitting method are used to modify the tooth thickness of the flexspline and analyze the performance indexes such as the assembly stress,transmission error,and fatigue life.Firstly,the conjugate tooth profile is solved based on the quadruple-circular-arc tooth profile and modified kinematic method.Then,based on the finite element radial deformation of the flexspline,the principle and characteristics of three modification methods are analyzed,and the modification amount of each section of the flexspline tooth is calculated.Finally,the influence of the three modification methods on the performance of the harmonic drive is compared.The results show that the radial deformation difference method can initially determine the modification amount.The minimum static assembly stress is 406.22 MPa by the major and minor axis fitting method.The ellipse fitting method has the best dynamic performance,small transmission error fluctuation,a peak-to-peak value of 3.060",and a maximum fatigue life of 10^(7.558)cycles.展开更多
The large volume expansion and rapid capacity attenuation of tin-based electrodes are the main factors limiting their commercial application.The reasonable design of electrode material structure is particularly import...The large volume expansion and rapid capacity attenuation of tin-based electrodes are the main factors limiting their commercial application.The reasonable design of electrode material structure is particularly important for improving its electrochemical performance.Herein,phosphorus-modified graphene encapsulated Sn_(6)O_(4)(OH)_(4)nanoparticles composite(P-Sn_(6)O_(4)(OH)_(4)@RGO)with crystalline-amorphous heterostructure has been successfully designed and prepared.The design of crystalline-amorphous structure has largely enhanced the active sites,and the construction of a graphene encapsulation structure has greatly alleviated volume expansion.Notably,P-Sn_(6)O_(4)(OH)_(4)@RGO obtained an excellent high-rate longterm cycling performance for lithium-ion batteries anode,reaching a high specific capacity of 970 m Ah/g at 1.0 A/g after 1450 cycles.This work demonstrates that restructuring the electrode material's structure and phase through phosphorus modification can effectively improve the electrochemical performance of tin-based electrode materials.展开更多
Based on Newton ' s second law,the bend-torsion-shaft coupling nonlinear dynamic model and equations of power split gear transmission system are established.According to the principle of tooth profile modification...Based on Newton ' s second law,the bend-torsion-shaft coupling nonlinear dynamic model and equations of power split gear transmission system are established.According to the principle of tooth profile modification,the tooth profile modification is considered as time-varying gear backlash function acting along the line of action.Then the dynamic functions are solved by using Runge-Kutta numerical method.After analyzing the effect of tooth profile modification quantity( TPMQ) and relative tooth profile modification length( TPML) to the nonlinear dynamic characteristics of power split gear transmission,the following conclusions are drawn:1 The TPMQ of a certain stage transmission affects the vibration of its own stage more significantly than the other stage,and the coupling effect between two stages can be ignored usually in the modification design;2 If the first stage TPMLs are less than 0.3,the influence of the first stage TPMLs to the first stage transmission vibration is much more greatly than the influence of the second stage TPMLs to the first stage transmission vibration,or else both the first and second stage TPMLs affect the first stage transmission vibration largely.The same is true for the second stage TPMLs,and the cutoff value is 0.2;3 The TPMQ affects the vibration of power split gear transmission system more principally than the TPML,and should be top-priority in the modification design.展开更多
A new swirl combustion device was designed and enhaneed,which realized the utilization of steel slag,achieved highly efficient and clean coal combustion,and simultaneously realized a fully elemental utilization of coa...A new swirl combustion device was designed and enhaneed,which realized the utilization of steel slag,achieved highly efficient and clean coal combustion,and simultaneously realized a fully elemental utilization of coal.The distribution laws of different sized particulate matter(PM)emission and the enrichment laws of elements in particles under diverse conditions(such as various excess air coefficients and different coal ratios)were systematically studied.The enrichments of PM under both non-staged and fuel-staged conditions were also investigated.The results indicated that fuel-staged combustion is more helpful in reducing PM emissions than non-staged combustion,and a suitable coal ratio is also beneficial for reducing PM emissions.The melted liquid steel slag drop captured the fly ash produced from pulverized combustion,thus reducing PM emission.The alkali metal elements(K,Na,and Mg),the trace elements(As and Ti).and S have an obvious enrichment tendency in PM]and PM2.5,A different coal ratio under fuel-staged combustion has a significant influence on the enrichment of Al,Si,Ca,and Fe in PM),whereas in PM2.5.PM4,and PM10,the effect of different coal ratios on the enrichment of each element is slight.展开更多
Ammonium aluminum carbonate hydroxide（AACH）, the precursor of α-Al2O3, was fabricated with NH4Al（SO4）2 and NH4HCO3 via a precipitation method. During the heat treatment AACH undergoes such a phase transformation ...Ammonium aluminum carbonate hydroxide（AACH）, the precursor of α-Al2O3, was fabricated with NH4Al（SO4）2 and NH4HCO3 via a precipitation method. During the heat treatment AACH undergoes such a phase transformation process: amorphous（350℃）→γ（700℃）→γ+θ（1050℃）→θ+α （1100℃）→α （1200℃）. Oxides, such as TiO2 sol and MgO sol were introduced into the precursor. Then both the α transformation temperature and the particle sizes of α-Al2O3 are decreased. The microstructures and its surface electronic properties of the powders are modified, and its dispersion property is improved. Doping TiO2 sol grains, AACH completes α phase transformation nearly at 1100℃, its first-order diameter is about 120nm. Doping with MgO sol grains, α phase transformation completes after being sintered at 1100℃.展开更多
基金supported by grants from the Major Projects of Health Science Research Foundation for Middle-Aged and Young Scientist of Fujian Province,China,No.2022ZQNZD01010010the National Natural Science Foundation of China,No.82371390Fujian Province Scientific Foundation,No.2023J01725(all to XC).
文摘The neuroinflammatory response mediated by microglial activation plays an important role in the secondary nerve injury of traumatic brain injury.The post-transcriptional modification of N^(6)-methyladenosine is ubiquitous in the immune response of the central nervous system.The fat mass and obesity-related protein catalyzes the demethylation of N^(6)-methyladenosine modifications on mRNA and is widely expressed in various tissues,participating in the regulation of multiple diseases’biological processes.However,the role of fat mass and obesity in microglial activation and the subsequent neuroinflammatory response after traumatic brain injury is unclear.In this study,we found that the expression of fat mass and obesity was significantly down-regulated in both lipopolysaccharide-treated BV2 cells and a traumatic brain injury mouse model.After fat mass and obesity interference,BV2 cells exhibited a pro-inflammatory phenotype as shown by the increased proportion of CD11b^(+)/CD86^(+)cells and the secretion of pro-inflammatory cytokines.Fat mass and obesity-mediated N^(6)-methyladenosine demethylation accelerated the degradation of ADAM17 mRNA,while silencing of fat mass and obesity enhanced the stability of ADAM17 mRNA.Therefore,down-regulation of fat mass and obesity expression leads to the abnormally high expression of ADAM17 in microglia.These results indicate that the activation of microglia and neuroinflammatory response regulated by fat mass and obesity-related N^(6)-methyladenosine modification plays an important role in the pro-inflammatory process of secondary injury following traumatic brain injury.
基金supported by the National Natural Science Foundation of China,No.22103055(to JG)the Natural Science Foundation of Hebei Province,No.F2024110001(to HC)Open Project of Tianjin Key Laboratory of Optoelectronic Detection Technology and System,Nos.2024LODTS215(to NL),2024LODTS216(to XS).
文摘In recent years,exosomes have garnered extensive attention as therapeutic agents and early diagnostic markers in neurodegenerative disease research.Exosomes are small and can effectively cross the blood-brain barrier,allowing them to target deep brain lesions.Recent studies have demonstrated that exosomes derived from different cell types may exert therapeutic effects by regulating the expression of various inflammatory cytokines,mRNAs,and disease-related proteins,thereby halting the progression of neurodegenerative diseases and exhibiting beneficial effects.However,exosomes are composed of lipid bilayer membranes and lack the ability to recognize specific target cells.This limitation can lead to side effects and toxicity when they interact with non-specific cells.Growing evidence suggests that surface-modified exosomes have enhanced targeting capabilities and can be used as targeted drug-delivery vehicles that show promising results in the treatment of neurodegenerative diseases.In this review,we provide an up-to-date overview of existing research aimed at devising approaches to modify exosomes and elucidating their therapeutic potential in neurodegenerative diseases.Our findings indicate that exosomes can efficiently cross the blood-brain barrier to facilitate drug delivery and can also serve as early diagnostic markers for neurodegenerative diseases.We introduce the strategies being used to enhance exosome targeting,including genetic engineering,chemical modifications(both covalent,such as click chemistry and metabolic engineering,and non-covalent,such as polyvalent electrostatic and hydrophobic interactions,ligand-receptor binding,aptamer-based modifications,and the incorporation of CP05-anchored peptides),and nanomaterial modifications.Research into these strategies has confirmed that exosomes have significant therapeutic potential for neurodegenerative diseases.However,several challenges remain in the clinical application of exosomes.Improvements are needed in preparation,characterization,and optimization methods,as well as in reducing the adverse reactions associated with their use.Additionally,the range of applications and the safety of exosomes require further research and evaluation.
基金supported by Applied Basic Research Joint Fund Project of Yunnan Province,No.202301AY070001-200Middle-aged Academic and Technical Training Project for High-Level Talents,No.202105AC160065+1 种基金Yunnan Clinical Medical Center for Neurological and Cardiovascular Diseases,No.YWLCYXZX2023300077Key Clinical Specialty of Neurology in Yunnan Province,No.300064(all to CL)。
文摘Research into lactylation modifications across various target organs in both health and disease has gained significant attention.Many essential life processes and the onset of diseases are not only related to protein abundance but are also primarily regulated by various post-translational protein modifications.Lactate,once considered merely a byproduct of anaerobic metabolism,has emerged as a crucial energy substrate and signaling molecule involved in both physiological and pathological processes within the nervous system.Furthermore,recent studies have emphasized the significant role of lactate in numerous neurological diseases,including Alzheimer's disease,Parkinson's disease,acute cerebral ischemic stroke,multiple sclerosis,Huntington's disease,and myasthenia gravis.The purpose of this review is to synthesize the current research on lactate and lactylation modifications in neurological diseases,aiming to clarify their mechanisms of action and identify potential therapeutic targets.As such,this work provides an overview of the metabolic regulatory roles of lactate in various disorders,emphasizing its involvement in the regulation of brain function.Additionally,the specific mechanisms of brain lactate metabolism are discussed,suggesting the unique roles of lactate in modulating brain function.As a critical aspect of lactate function,lactylation modifications,including both histone and non-histone lactylation,are explored,with an emphasis on recent advancements in identifying the key regulatory enzymes of such modifications,such as lactylation writers and erasers.The effects and specific mechanisms of abnormal lactate metabolism in diverse neurological diseases are summarized,revealing that lactate acts as a signaling molecule in the regulation of brain functions and that abnormal lactate metabolism is implicated in the progression of various neurological disorders.Future research should focus on further elucidating the molecular mechanisms underlying lactate and lactylation modifications and exploring their potential as therapeutic targets for neurological diseases.
基金supported by the National Natural Science Foundation of China(Nos.82573045,82460602,82560459)the Hainan Provincial Graduate Student Innovative Research Project(No.Qhys2024-440).
文摘Post-translational modifications(PTMs)regulate the occurrence and development of cancer,and lactylation modification is a new form of PTMs.Recent studies have found that lactic acid modification can regulate the immune tolerance of cancer cells.The classical theory holds that prostate apoptosis response-4(PAR-4)is a tumor suppressor protein.However,our recent research has found that PAR-4 has a biological function of promoting cancer in hepatocellular carcinoma(HCC),and our analysis shows that PAR-4 can be modified of lactic acid.These research evidences suggest that PAR-4 lactylation modification may drive immune tolerance in HCC.Therefore,inhibiting PAR-4 lactylation modification is very likely to increase the sensitivity of HCC to immunotherapy.
文摘Aberrant RNA modification has been linked to the pathogenesis of various diseases;however,its specific molecular mechanisms in spinal cord injury remain poorly understood.The objective of this study was to explore RNA modification-related biomarkers of spinal cord injury.The mRNA expression profiles of mice with spinal cord injury were retrieved from the Gene Expression Omnibus(GEO)database(GSE18179).We identified 185 differentially expressed genes using bioinformatics approaches.Functional enrichment analysis demonstrated aberrant activation or inhibition of common metabolism-related pathways,including sulfur metabolism and steroid biosynthesis,in mice with spinal cord injury.An integrated strategy comprising weighted gene co-expression network analysis,a random forest model,a support vector machine model,and a generalized linear model was employed to identify four genes whose aberrant RNA modification was linked to spinal cord injury:Elovl6,Idi1,Sqle,and Stbd1.We verified the expression levels and diagnostic performance of these four genes in the original training dataset and mouse samples via receiver operating characteristic curve analysis.Quantitative reverse transcription-polymerase chain reaction demonstrated variations in the mRNA levels of the four genes between the Sham and spinal cord injury groups at different time points following injury.We also constructed microRNA-mRNA and transcription factor-mRNA interaction networks using Cytoscape.Additionally,we evaluated the proportions of 22 types of immune cells in the spinal cords of mice using the CIBERSORT tool,revealing significant alterations in the numbers of memory B cells,resting dendritic cells,M0 macrophages,activated mast cells,resting mast cells,and CD8+T cells in spinal cord injury mice compared with Sham controls.Microglia and T cells were identified as key cell types by single-cell sequencing analysis.These findings provide new directions for the development of RNA modification-related therapeutic strategies for spinal cord injury and suggest that Elovl6,Idi1,Sqle,and Stbd1 are potential biomarkers of spinal cord injury.
文摘Real time prosody modification involves changing the prosody parameters such as pitch, duration and intensity of speech in real time without affecting the intelligibility and naturalness. In this paper prosody modification is performed using instants of significant excitation (ISE) of the vocal tract system during production of speech. In the conventional prosody modification system the ISE are computed using group delay function, and it is computationally intensive task. In this paper, we propose computationally efficient methods to determine the ISE suitable for prosody modification in interactive (real time) applications. The overall computational time for the prosody modification by using the proposed method is compared with the conventional prosody modification method which uses the group delay function for computing the ISE.
文摘We are sorry for the mistakes of Affiliation,"a State Key Laboratory of Advanced Fiber Materials,Center for Advanced Low-Dimension Materials,Donghua University,Shanghai 201620,China"should be replaced by"a State Key Laboratory of Advanced Fiber Materials,Center for Advanced Low-Dimension Materials,College of Materials Science and Engineering,Donghua University,Shanghai 201620,China".We apologized for the inconvenience caused by this error.
文摘Before-and-after methods have been effectively used in the road safety studies to estimate Crash Modification Factors (CMFs) of individual treatments as well as the multiple treatments on roadways. Since the common practice is to apply multiple treatments on road segments, it is important to have a method to estimate CMFs of individual treatment so that the effect of each treatment towards improving the road safety can be identified. Even though there are methods introduced by researchers to combine multiple CMFs or to isolate the safety effectiveness of individual treatment from CMFs developed for multiple treatments, those methods have to be tested before using them. This study considered two multiple treatments namely 1) Safety edge with lane widening 2) Adding 2 ft paved shoulders with shoulder rumble strips and/or asphalt resurfacing. The objectives of this research are to propose a regression-based method to estimate individual CMFs estimate CMFs using before-and-after Empirical Bayes method and compare the results. The results showed that having large sample size gives accurate predictions with smaller standard error and p-values of the considered treatments. Also, results obtained from regression method are similar to the EB method even though the values are not exactly the same. Finally, it was seen that the safety edge treatment reduces crashes by 15% - 25% and adding 2 ft shoulders with rumble strips reduces crashes by 25% - 49%.
基金supported by the National Natural Science Foundation of China,Nos.82030071(to JH),82272495(to YC)Science and Technology Major Project of Changsha,No.kh2103008(to JH)Graduate Students’Independent Innovative Projects of Hunan Province,No.CX20230311(to YJ)。
文摘Spinal cord injury typically causes corticospinal tract disruption.Although the disrupted corticospinal tract can self-regenerate to a certain degree,the underlying mechanism of this process is still unclear.N6-methyladenosine(m^(6)A)modifications are the most common form of epigenetic regulation at the RNA level and play an essential role in biological processes.However,whether m^(6)A modifications participate in corticospinal tract regeneration after spinal cord injury remains unknown.We found that expression of methyltransferase 14 protein(METTL14)in the locomotor cortex was high after spinal cord injury and accompanied by elevated m^(6)A levels.Knockdown of Mettl14 in the locomotor cortex was not favorable for corticospinal tract regeneration and neurological recovery after spinal cord injury.Through bioinformatics analysis and methylated RNA immunoprecipitation-quantitative polymerase chain reaction,we found that METTL14 regulated Trib2 expression in an m^(6)A-regulated manner,thereby activating the mitogen-activated protein kinase pathway and promoting corticospinal tract regeneration.Finally,we administered syringin,a stabilizer of METTL14,using molecular docking.Results confirmed that syringin can promote corticospinal tract regeneration and facilitate neurological recovery by stabilizing METTL14.Findings from this study reveal that m^(6)A modification is involved in the regulation of corticospinal tract regeneration after spinal cord injury.
文摘Viruses and their hosts have co-evolved for million years. In order to successfully replicate their genome, viruses need to usurp the biosynthetic machinery of the host cell. Depending on the complexity and the nature of the genome, replication might involve or not a relatively large subset of viral products, in addition to a number of host cell factors, and take place in several subcellular compartments, including the nucleus,the cytoplasm, as well as virus-induced, rearranged membranes. Therefore viruses need to ensure the correct subcellular localization of their effectors and to be capable of disguising from the cellular defensive mechanisms. In addition, viruses are capable of exploiting host cell activities, by modulating their post-translational modification apparatus, resulting in profound modifications in the function of cellular and viral products. Not surprisingly infection of host cells by these parasites can lead to alterations of cellular differentiation and growing properties, with important pathogenic consequences. In the present hot topic highlight entitled "Reprogramming the host: modification of cell functions upon viral infection", a number of leading virologists and cell biologist thoroughly describe recent advances in our understanding of how viruses modulate cellular functions to achieve successful replication and propagation at the expenses of human cells.
基金Supported by Design and Performance Study of High-flux Metal Hydride Reactor Based on the Bionic Optimization(2078262)the‘Four-Chain’Integration Project at the Qinchuangyuan Chief Platform(S2025-YF-ZDXM)。
文摘As one of the most promising new energy sources,hydrogen energy is expected to usher in a full-fledged“hydrogen economy”in the 21st century.Compared with traditional high-pressure gaseous and cryogenic liquid hydrogen storage methods,solid-state chemical hydrogen storage shows significant advantages in safety,high efficiency,and cost-effectiveness.Magnesium-based lightweight hydrogen storage materials have attracted widespread attention due to their high gravimetric hydrogen storage density(7.6%)and favorable reversibility.However,their sluggish reaction kinetics and stringent operating conditions(with H2 release temperatures exceeding 350°C and H2 absorption pressures above 4 MPa)pose major challenges for practical applications.Domestic and international researchers have conducted in-depth studies to address these issues,achieving substantial progress in the modification of magnesium-based hydrogen storage alloys.This paper systematically elaborates on major modification techniques such as alloying,nanostructuring,and catalytic material doping,providing a comprehensive analysis of the strengths and limitations of each approach.Furthermore,it offers prospects for the future development of magnesium-based hydrogen storage materials by integrating current theoretical and experimental research findings.
文摘Biomass-derived carbon materials are favored for their abundance and sustainability,and ease of preparation and modification.By surface activation and modification they can have a good electrical conductivity,excellent catalytic activity,a remarkable adsorption capacity,and different interfacial physicochemical functionalities.Surface-modified biochars have found wide applications in energy storage,environmental remediation,and catalysis.However,achieving precise and controllable modification of their active sites remains a challenge.Recent advances and future prospects for controlling their surface morphology,defect engineering,and surface coating strategies,with particular attention to their means of fabrication,are reviewed.
基金financial support by the National Key Research and Development Program of China(No.2023YFC2907801)the Hunan Provincial Natural Science Foundation of China(No.2023JJ40760)the Scientific and Technological Project of Yunnan Precious Metals Laboratory,China(No.YPML-2023050276)。
文摘Bentonite is a necessary binder in producing pellets.Its excessive use reduces the iron grade of pellets and increases production costs.Minimizing bentonite dosage is essential for producing high-quality iron ore pellets.Addressing the gap in the application of organically-intercalated modified bentonite in the pelletizing field,this study introduces an innovative modification process for bentonite that employs the synergistic effect of mechanical force and dimethyl sulfoxide to enhance the intercalation of organic compounds within bentonite,thus significantly enhancing its binding performance.The colloid value and swell capacity of modified bentonite(98.5 m L/3g and 55.0 m L/g)were much higher than the original bentonite(90.5 m L/3g and 17.5 m L/g).With the decrease of bentonite dosage from1.5wt%to 1.0wt%,the drop number of green pellets from a height of 0.5 m and the compressive strengths of roasted pellets using the modified bentonite(6.0 times and 2916 N per pellet)were significantly higher than those of the original bentonite(4.0 times and 2739 N per pellet).This study provides a comprehensive analysis of the intercalation modification mechanism of bentonite,offering crucial technical insights for the development of high-performance modified bentonite as iron ore pellet binders.
基金financially supported by the National Nature Science Foundation of China (62504130)National Key Research and Development Program of China (2018YFB0704100)+3 种基金the Key university laboratory of highly efficient utilization of solar energy and sustainable development of Guangdong (Y01256331)the Technology Development Project of Henan Province (252102240047)the Pico Center at SUSTech CRF which receives support from the Presidential FundDevelopment and Reform Commission of Shenzhen Municipality
文摘Two-step-processed(TSP)inverted p-i-n perovskite solar cells(PSCs)have demonstrated significant promise in tandem applications.However,the power conversion efficiency(PCE)of TSP p-i-n PSCs rarely exceeds 24%.Here,we demonstrate that TSP perovskite films exhibit a vertically gradient distribution of residual PbI_(2)clusters,which form Schottky heterojunctions with the perovskite,leading to substantial interfacial energy-level mismatches within NiO_(x)-based TSP p-i-n PSCs.These limitations were effectively addressed via a vertical interfacial engineering enabled by dual-interface modification incorporating tin trifluoromethanesulfonate(Sn(OTF)_(2))and 4-Fluorophenylethylamine chloride(F-PEA)at the NiO_(x)/perovskite and perovskite/C60 interfaces,respectively.The functional Sn(OTF)_(2)not only enhances the conductivity of NiO_(x)films but also suppresses ion migration,while inducing the formation of a Pb-Sn mixed perovskite interlayer that precisely regulates the energy level at the NiO_(x)/perovskite interface.Complementally,F-PEA post-treatment effectively converts surface residual PbI_(2)clusters into a 2D perovskite capping layer,which simultaneously passivates surface defects and enhances energy-level alignment at the perovskite/C60 interface.Consequently,the optimized NiO_(x)-based TSP p-i-n PSCs achieve a notable PCE of 25.6%with superior operational stability.This study elucidates the underlying mechanisms limiting the efficiency of TSP p-i-n PSCs,while establishing design principles for these devices targeting 26%efficiency.
基金supported by the National Natural Science Foundation of China(Nos.U2341249,12005076,22205112)the Fundamental Research Funds for the Central Universities(No.2025201012)。
文摘The presence of a surface oxide film(B_(2)O_(3))on boron(B)particles significantly compromises their combustion efficiency and kinetic performance in fuel-rich solid propellants.This study proposes an innovative continuous modification strategy combining non-thermal plasma(NTP)etching with fluorocarbon passivation.Characterization and kinetic analysis revealed that reactive plasma species—including atomic hydrogen(H),electronically excited molecular hydrogen(H_(2)^(*)),vibrationally excited molecular hydrogen(H_(2)v),and hydrogen ions(H^(+))—dominate the reduction of B_(2)O_(3)through lowering the transition energy barrier and shifting the reaction spontaneity.Subsequent argon plasma fragmentation of C_(8)F_(18)generates fluorocarbon radicals that form conformal passivation coatings(thickness:7 nm)on purified boron surfaces.The modified boron particles exhibit 37.5℃lower exothermic peak temperature and 27.2%higher heat release(14.8 kJ/g vs.11.6 kJ/g)compared to untreated counterparts.Combustion diagnostics reveal 194%increase in maximum flame height(135.10 mm vs.46.03 mm)and 134%enhancement in flame propagation rate(4.44 cm/s vs.1.90 cm/s).This NTP-based surface engineering approach establishes a scalable pathway for developing highperformance boron-based energetic composites.
文摘In order to eliminate the meshing interference between the flexspline and circular spline after the taper deformation of the flexspline,the radial deformation difference method,major and minor axis fitting method,and ellipse fitting method are used to modify the tooth thickness of the flexspline and analyze the performance indexes such as the assembly stress,transmission error,and fatigue life.Firstly,the conjugate tooth profile is solved based on the quadruple-circular-arc tooth profile and modified kinematic method.Then,based on the finite element radial deformation of the flexspline,the principle and characteristics of three modification methods are analyzed,and the modification amount of each section of the flexspline tooth is calculated.Finally,the influence of the three modification methods on the performance of the harmonic drive is compared.The results show that the radial deformation difference method can initially determine the modification amount.The minimum static assembly stress is 406.22 MPa by the major and minor axis fitting method.The ellipse fitting method has the best dynamic performance,small transmission error fluctuation,a peak-to-peak value of 3.060",and a maximum fatigue life of 10^(7.558)cycles.
基金supported by the Natural Science Foundation of Shandong Province(Nos.ZR2024QE450,ZR2024QB302 and ZR2024QB004)the Taishan Scholars and Young Experts Program of Shandong Province(No.tsqn202211249)Research Program of Qilu Institute of Technology(Nos.QIT 23TP019,QIT23TP010 and QIT24NN007)。
文摘The large volume expansion and rapid capacity attenuation of tin-based electrodes are the main factors limiting their commercial application.The reasonable design of electrode material structure is particularly important for improving its electrochemical performance.Herein,phosphorus-modified graphene encapsulated Sn_(6)O_(4)(OH)_(4)nanoparticles composite(P-Sn_(6)O_(4)(OH)_(4)@RGO)with crystalline-amorphous heterostructure has been successfully designed and prepared.The design of crystalline-amorphous structure has largely enhanced the active sites,and the construction of a graphene encapsulation structure has greatly alleviated volume expansion.Notably,P-Sn_(6)O_(4)(OH)_(4)@RGO obtained an excellent high-rate longterm cycling performance for lithium-ion batteries anode,reaching a high specific capacity of 970 m Ah/g at 1.0 A/g after 1450 cycles.This work demonstrates that restructuring the electrode material's structure and phase through phosphorus modification can effectively improve the electrochemical performance of tin-based electrode materials.
基金Sponsored by the National Natural Science Foundation of China(Grant No.2009AA04Z404)
文摘Based on Newton ' s second law,the bend-torsion-shaft coupling nonlinear dynamic model and equations of power split gear transmission system are established.According to the principle of tooth profile modification,the tooth profile modification is considered as time-varying gear backlash function acting along the line of action.Then the dynamic functions are solved by using Runge-Kutta numerical method.After analyzing the effect of tooth profile modification quantity( TPMQ) and relative tooth profile modification length( TPML) to the nonlinear dynamic characteristics of power split gear transmission,the following conclusions are drawn:1 The TPMQ of a certain stage transmission affects the vibration of its own stage more significantly than the other stage,and the coupling effect between two stages can be ignored usually in the modification design;2 If the first stage TPMLs are less than 0.3,the influence of the first stage TPMLs to the first stage transmission vibration is much more greatly than the influence of the second stage TPMLs to the first stage transmission vibration,or else both the first and second stage TPMLs affect the first stage transmission vibration largely.The same is true for the second stage TPMLs,and the cutoff value is 0.2;3 The TPMQ affects the vibration of power split gear transmission system more principally than the TPML,and should be top-priority in the modification design.
文摘A new swirl combustion device was designed and enhaneed,which realized the utilization of steel slag,achieved highly efficient and clean coal combustion,and simultaneously realized a fully elemental utilization of coal.The distribution laws of different sized particulate matter(PM)emission and the enrichment laws of elements in particles under diverse conditions(such as various excess air coefficients and different coal ratios)were systematically studied.The enrichments of PM under both non-staged and fuel-staged conditions were also investigated.The results indicated that fuel-staged combustion is more helpful in reducing PM emissions than non-staged combustion,and a suitable coal ratio is also beneficial for reducing PM emissions.The melted liquid steel slag drop captured the fly ash produced from pulverized combustion,thus reducing PM emission.The alkali metal elements(K,Na,and Mg),the trace elements(As and Ti).and S have an obvious enrichment tendency in PM]and PM2.5,A different coal ratio under fuel-staged combustion has a significant influence on the enrichment of Al,Si,Ca,and Fe in PM),whereas in PM2.5.PM4,and PM10,the effect of different coal ratios on the enrichment of each element is slight.
文摘Ammonium aluminum carbonate hydroxide（AACH）, the precursor of α-Al2O3, was fabricated with NH4Al（SO4）2 and NH4HCO3 via a precipitation method. During the heat treatment AACH undergoes such a phase transformation process: amorphous（350℃）→γ（700℃）→γ+θ（1050℃）→θ+α （1100℃）→α （1200℃）. Oxides, such as TiO2 sol and MgO sol were introduced into the precursor. Then both the α transformation temperature and the particle sizes of α-Al2O3 are decreased. The microstructures and its surface electronic properties of the powders are modified, and its dispersion property is improved. Doping TiO2 sol grains, AACH completes α phase transformation nearly at 1100℃, its first-order diameter is about 120nm. Doping with MgO sol grains, α phase transformation completes after being sintered at 1100℃.
