The Pearl River Estuary(PRE)is one of China’s busiest shipping hubs and fishery production centers,as well as a region with abundant island tourism and wind energy resources,which calls for accurate short-term wind f...The Pearl River Estuary(PRE)is one of China’s busiest shipping hubs and fishery production centers,as well as a region with abundant island tourism and wind energy resources,which calls for accurate short-term wind forecasts.First,this study evaluated three operational numerical models,i.e.,ECMWF-EC,NCEP-GFS,and CMA-GD,for their ability to predict short-term wind speed over the PRE against in-situ observations during 2018-2021.Overall,ECMWF-EC out-performs other models with an average RMSE of 2.24 m s^(-1)and R of 0.57,but the NCEP-GFS performs better in the case of strong winds.Then,various bias correction and multi-model ensemble(MME)methods are used to perform the deterministic post-processing using a local and lead-specific scheme.Two-factor model output statistics(MOS2)is the optimal bias correction method for reducing(increasing)the overall RMSE(R)to 1.62(0.70)m s^(-1),demonstrating the benefits of considering both initial and lead-specific information.Intercomparison of MME results reveals that Multiple linear regression(MLR)presents superior skills,followed by random forest(RF),but it is slightly inferior to MOS2,particularly for the first few forecasting hours.Furthermore,the incorporation of additional features in MLR reduces the overall RMSE to 1.53 m s^(-1)and increases R to 0.74.Similarly,RF presents comparable results,and both outperform MOS2 in terms of correcting their deficiencies at the first few lead hours and limiting the error growth rate.Despite the satisfactory skill of deterministic post-processing techniques,they are unable to achieve a balanced performance between mean and extreme statistics.This highlights the necessity for further development of probabilistic forecasts.展开更多
The purpose of this paper is to explore the application of large language models(LLMs)in legal case retrieval and to evaluate their potential for providing legal professionals with more efficient work aids.Currently,a...The purpose of this paper is to explore the application of large language models(LLMs)in legal case retrieval and to evaluate their potential for providing legal professionals with more efficient work aids.Currently,although pre-trained models have made great progress in legal case retrieval,they are often limited to specific types of law(e.g.,criminal law,civil law,etc.)and lack the ability to generalize across different types of law.Moreover,most models can only deal with a single task,whereas the legal case retrieval task requires a model to have a superb comprehension of legal texts,involving multiple subtasks and requiring multitasking capabilities.Therefore,the large language model,which has super generalization and multitasking ability,can solve the above problems.In order to explore the application of large language models for legal case retrieval in the legal domain,this paper evaluates a series of emerging large language models,including multilingual models,homegrown large models,and models specifically designed for the legal domain.These models are used to retrieve legal cases and its associated subtasks.Based on the Supreme People’s Court definition,the legal case retrieval task is broken down into seven subtasks:event detection,fact generation,trigger word extraction,keyword extraction,summarization,dispute focus identification,and reasoning generation.Using a variety of evaluation metrics,the experiments demonstrated that these emerging models have significant potential in the field of legal case retrieval,even with few shot samples.The research in this paper not only introduces new ideas in the field of legal case retrieval,but also empirically verifies the potential of LLMs to improve the quality and efficiency of retrieval.It proves the value of large language models in this field and is expected to significantly enhance the efficiency of legal practitioners,as well as promote the consistency and fairness of legal judgments through the use of emerging technologies.展开更多
With the rapid development of generative artificial intelligence technologies,represented by large language models,university-level computer science education is undergoing a critical transition-from knowledge-based i...With the rapid development of generative artificial intelligence technologies,represented by large language models,university-level computer science education is undergoing a critical transition-from knowledge-based instruction to competency-oriented teaching.A postgraduate student competency evaluation model can serve as a framework to organize and guide both teaching and research activities at the postgraduate level.A number of relevant research efforts have already been conducted in this area.Graduate education plays a vital role not only as a continuation and enhancement of undergraduate education but also as essential preparation for future research endeavors.An analysis of the acceptance of competency evaluation models refers to the assessment of how various stakeholders perceive the importance of different components within the model.Investigating the degree of acceptance among diverse groups-such as current undergraduate students,current postgraduate students,graduates with less than three years of work experience,and those with more than three years of work experience-can offer valuable insights for improving and optimizing postgraduate education and training practices.展开更多
With the continuous development of the nursing discipline,standardized nurse training has always been a crucial link in the development of nursing science and plays an irreplaceable role in talent cultivation.However,...With the continuous development of the nursing discipline,standardized nurse training has always been a crucial link in the development of nursing science and plays an irreplaceable role in talent cultivation.However,in the current standardized training for some nurses,there are problems such as the simplification of nursing skill evaluation models and insufficient post competence of nurses.Therefore,optimizing the training model for nursing talents has become an inevitable measure.The problem-based learning(PBL)method and the Direct Observation of Procedural Skills(DOPS)evaluation model provide new directions and guidance for the development of training.Against this background,this paper explores effective approaches for standardized nurse training,starting from basic concepts and gradually delving into specific practical paths,aiming to improve the quality of talent cultivation and provide valuable references for other researchers.展开更多
Objective:To explore the application value of a new empowerment teaching method based on Kirkpatrick’s evaluation model in teaching Chinese medicine nursing in otorhinolaryngology.Methods:60 nurses who practiced in t...Objective:To explore the application value of a new empowerment teaching method based on Kirkpatrick’s evaluation model in teaching Chinese medicine nursing in otorhinolaryngology.Methods:60 nurses who practiced in the otolaryngology department of our hospital from June 2022 to October 2024 were included in the study and equally divided into two groups using a convenient sampling method.30 nurses who chose traditional Chinese medicine skill teaching management were included in the control group,and 30 nurses who chose the new empowerment teaching method based on Kirkpatrick’s evaluation model were included in the observation group.Relevant indicators such as clinical teaching environment perception,theoretical knowledge scores of Chinese medicine nursing,and excellent rate of practical operation assessment were compared.Results:The nurses in the observation group had higher scores for clinical teaching environment perception than the control group(P<0.05).However,the midterm and final exam scores for theoretical knowledge of Chinese medicine nursing were higher in the observation group than in the control group(P<0.05).Compared with the control group,the observation group had a higher excellent rate of practical operation assessment(93.33%>73.33%)and a higher Chinese medicine nursing ability score[(215.69±19.73)points>(184.87±15.66)points](P<0.05).Conclusion:Applying the new empowerment teaching method based on Kirkpatrick’s evaluation model to Chinese medicine nursing teaching in otolaryngology can help nurses understand the theoretical knowledge of Chinese medicine nursing and optimize the clinical teaching environment,thereby promoting their practical skills and Chinese medicine nursing abilities.展开更多
This paper proposes a multivariate data fusion based quality evaluation model for software talent cultivation.The model constructs a comprehensive ability and quality evaluation index system for college students from ...This paper proposes a multivariate data fusion based quality evaluation model for software talent cultivation.The model constructs a comprehensive ability and quality evaluation index system for college students from a perspective of engineering course,especially of software engineering.As for evaluation method,relying on the behavioral data of students during their school years,we aim to construct the evaluation model as objective as possible,effectively weakening the negative impact of personal subjective assumptions on the evaluation results.展开更多
Vector winds play a crucial role in weather and climate,as well as the effective utilization of wind energy resources.However,limited research has been conducted on treating the wind field as a vector field in the eva...Vector winds play a crucial role in weather and climate,as well as the effective utilization of wind energy resources.However,limited research has been conducted on treating the wind field as a vector field in the evaluation of numerical weather prediction models.In this study,the authors treat vector winds as a whole by employing a vector field evaluation method,and evaluate the mesoscale model of the China Meteorological Administration(CMA-MESO)and ECMWF forecast,with reference to ERA5 reanalysis,in terms of multiple aspects of vector winds over eastern China in 2022.The results show that the ECMWF forecast is superior to CMA-MESO in predicting the spatial distribution and intensity of 10-m vector winds.Both models overestimate the wind speed in East China,and CMA-MESO overestimates the wind speed to a greater extent.The forecasting skill of the vector wind field in both models decreases with increasing lead time.The forecasting skill of CMA-MESO fluctuates more and decreases faster than that of the ECMWF forecast.There is a significant negative correlation between the model vector wind forecasting skill and terrain height.This study provides a scientific evaluation of the local application of vector wind forecasts of the CMA-MESO model and ECMWF forecast.展开更多
Solar energy is a pivotal clean energy source in the transition to carbon neutrality from fossil fuels.However,the intermittent and stochastic characteristics of solar radiation pose challenges for accurate simulation...Solar energy is a pivotal clean energy source in the transition to carbon neutrality from fossil fuels.However,the intermittent and stochastic characteristics of solar radiation pose challenges for accurate simulation and prediction.Accurately simulating and predicting solar radiation and its variability are crucial for optimizing solar energy utilization.This study conducted simulation experiments using the WRF-Solar model from 25 June to 25 July 2022,to evaluate the accuracy and performance of the simulated solar radiation across China.The simulations covered the whole country with a grid spacing of 27 km and were compared with ground observation network data from the Chinese Ecosystem Research Network.The results indicated that WRF-Solar can accurately capture the spatiotemporal patterns of global horizontal irradiance over China,but there is still an overestimation of solar radiation,and the model underestimates the total cloud cover.The root-mean-square error ranged from 92.83 to 188.13 W m^(-2) and the mean bias(MB)ranged from 21.05 to 56.22 W m^(-2).The simulation showed the smallest MB at Lhasa on the Qinghai–Tibet Plateau,while the largest MB was observed in Southeast China.To enhance the accuracy of solar radiation simulation,the authors compared the Fast All-sky Radiation Model for Solar with the Rapid Radiative Transfer Model for General Circulation Models and found that the former provides better simulation.展开更多
Based on the C-Coupler platform,the semi-unstructured Climate System Model,Synthesis Community Integrated Model version 2(SYCIM2.0),has been developed at the School of Atmospheric Sciences,Sun Yat-sen University.SYCIM...Based on the C-Coupler platform,the semi-unstructured Climate System Model,Synthesis Community Integrated Model version 2(SYCIM2.0),has been developed at the School of Atmospheric Sciences,Sun Yat-sen University.SYCIM2.0 aims to meet the demand for seamless climate prediction through accurate climate simulations and projections.This paper provides an overview of SYCIM2.0 and highlights its key features,especially the coupling of an unstructured ocean model and the tuning process.An extensive evaluation of its performance,focusing on the East Asian Summer Monsoon(EASM),is presented based on long-term simulations with fixed external forcing.The results suggest that after nearly 240 years of integration,SYCIM2.0 achieves a quasi-equilibrium state,albeit with small trends in the net radiation flux at the top-of-atmosphere(TOA)and Earth’s surface,as well as with global mean near-surface temperatures.Compared to observational and reanalysis data,the model realistically simulates spatial patterns of sea surface temperature(SST)and precipitation centers to include their annual cycles,in addition to the lower-level wind fields in the EASM region.However,it exhibits a weakened and eastward-shifted Western Pacific Subtropical High(WPSH),resulting in an associated precipitation bias.SYCIM2.0 robustly captures the dominant mode of the EASM and its close relationship with the El Niño-Southern Oscillation(ENSO)but exhibits relatively poor performance in simulating the second leading mode and the associated air–sea interaction processes.Further comprehensive evaluations of SYCIM2.0 will be conducted in future studies.展开更多
Snow cover over the Tibetan Plateau(TP)(TPSC)has garnered significant attention as a crucial indicator of climate change,along with its variations and related climate processes.However,due to the complex terrain of th...Snow cover over the Tibetan Plateau(TP)(TPSC)has garnered significant attention as a crucial indicator of climate change,along with its variations and related climate processes.However,due to the complex terrain of the TP,most numerical models exhibit notable uncertainty in simulating snow conditions in this area.This study evaluates historical snow simulations and related climate anomalies over the TP in numerical models from phase 6 of the Coupled Model Intercomparison Project(CMIP6).The CMIP6 model simulations are compared with two observation-based products across different seasons and temporal scales,and the results indicate that the CMIP6 multimodel ensemble(MME)mean reasonably captures the spatial distribution of the annual and seasonal climatological mean TP snow,albeit with weaker magnitudes compared to observations.The CMIP6 MME performs better over the western TP than the eastern regions,showing a higher reproducibility of the long-term warming trends and declining snow cover trends,partly due to the atmospheric circulation anomalies related to global warming.Additionally,some CMIP6 models successfully capture the interannual variability of TPSC and its relationship with associated climate factors.Our work emphasizes the importance of CMIP6 model selection and pays attention to data reliability in interpreting CMIP6 model results across different TP regions when studying snow cover variations and climate effects using numerical models.展开更多
Numerical models are crucial for quantifying the ocean-atmosphere interactions associated with the El Niño-Southern Oscillation(ENSO)phenomenon in the tropical Pacific.Current coupled models often exhibit signifi...Numerical models are crucial for quantifying the ocean-atmosphere interactions associated with the El Niño-Southern Oscillation(ENSO)phenomenon in the tropical Pacific.Current coupled models often exhibit significant biases and inter-model differences in simulating ENSO,underscoring the need for alternative modeling approaches.The Regional Ocean Modeling System(ROMS)is a sophisticated ocean model widely used for regional studies and has been coupled with various atmospheric models.However,its application in simulating ENSO processes on a basin scale in the tropical Pacific has not been explored.For the first time,this study presents the development of a basin-scale hybrid coupled model(HCM)for the tropical Pacific,integrating ROMS with a statistical atmospheric model that captures the interannual relationships between sea surface temperature(SST)and wind stress anomalies.The HCM is evaluated for its capability to simulate the annual mean,seasonal,and interannual variations of the oceanic state in the tropical Pacific.Results demonstrate that the model effectively reproduces the ENSO cycle,with a dominant oscillation period of approximately two years.The ROMS-based HCM developed here offers an efficient and robust tool for investigating climate variability in the tropical Pacific.展开更多
An internal defect meter is an instrument to detect the internal inclusion defects of cold-rolled strip steel.The detection accuracy of the equipment can be evaluated based on the similarity of the multiple detection ...An internal defect meter is an instrument to detect the internal inclusion defects of cold-rolled strip steel.The detection accuracy of the equipment can be evaluated based on the similarity of the multiple detection data obtained for the same steel coil.Based on the cosine similarity model and eigenvalue matrix model,a comprehensive evaluation method to calculate the weighted average of similarity is proposed.Results show that the new method is consistent with and can even replace artificial evaluation to realize the automatic evaluation of strip defect detection results.展开更多
In the concurrent extraction of coal and gas,the quantitative assessment of evolving characteristics in mining-induced fracture networks and mining-enhanced permeability within coal seams serves as the cornerstone for...In the concurrent extraction of coal and gas,the quantitative assessment of evolving characteristics in mining-induced fracture networks and mining-enhanced permeability within coal seams serves as the cornerstone for effective gas extraction.However,representing mining-induced fracture networks from a three-dimensional(3D)sight and developing a comprehensive model to evaluate the anisotropic mining-enhanced permeability characteristics still pose significant challenges.In this investigation,a field experiment was undertaken to systematically monitor the evolution of borehole fractures in the coal mass ahead of the mining face at the Pingdingshan Coal Mining Group in China.Using the testing data of borehole fracture,the mining-induced fracture network at varying distances from the mining face was reconstructed through a statistical reconstruction method.Additionally,utilizing fractal theory,a model for the permeability enhancement rate(PER)induced by mining was established.This model was employed to quantitatively depict the anisotropic evolution patterns of PER as the mining face advanced.The research conclusions are as follows:(1)The progression of the mining-induced fracture network can be classified into the stage of rapid growth,the stage of stable growth,and the stage of weak impact;(2)The PER of mining-induced fracture network exhibited a typical progression that can be characterized with slow growth,rapid growth and significant decline;(3)The anisotropic mining-enhanced permeability of the reconstructed mining-induced fracture networks were significant.The peak PER in the vertical direction of the coal seam is 6.86 times and 4446.38 times greater than the direction perpendicular to the vertical thickness and the direction parallel to the advancement of the mining face,respectively.This investigatione provides a viable approach and methodology for quantitatively assessing the anisotropic PER of fracture networks induced during mining,in the concurrent exploitation of coal and gas.展开更多
Through the investigation of the species of street trees located along the main urban roads in Hefei City,a total of 22 species were selected,belonging to 16 families and 22 genera,with the Sapindaceae family being th...Through the investigation of the species of street trees located along the main urban roads in Hefei City,a total of 22 species were selected,belonging to 16 families and 22 genera,with the Sapindaceae family being the most prevalent.In this study,the Analytic Hierarchy Process(AHP)was employed to assess the comprehensive value of 22 species of street trees applied along the main urban roads in Hefei City.Fourteen evaluation criteria were selected from four categories:morphological indices,functional indices,resistance indices,and management indices,to develop a comprehensive evaluation model.Based on a composite score derived from 22 street trees,these trees were classified into three distinct grades.Grade I(L≥3.0)exhibited a high comprehensive application value in Hefei City and included 6 tree species,such asPlatanus.Grade II(2.5≤L<3.0)also demonstrated a high comprehensive application value,comprising 15 tree species,includingCatalpabungei.In contrast,grade III(L<2.5)indicated a general comprehensive application value,represented by a single species,Cedrusdeodara.The evaluation results can offer theoretical insights for the selection of urban street trees.展开更多
In the era of AI,especially large models,the importance of open source has become increasingly prominent.First,open source allows innovation to avoid starting from scratch.Through iterative innovation,it promotes tech...In the era of AI,especially large models,the importance of open source has become increasingly prominent.First,open source allows innovation to avoid starting from scratch.Through iterative innovation,it promotes technical exchanges and learning globally.Second,resources required for large model R&D are difficult for a single institution to obtain.The evaluation of general large models also requires the participation of experts from various industries.Third,without open source collaboration,it is difficult to form a unified upper-layer software ecosystem.Therefore,open source has become an important cooperation mechanism to promote the development of AI and large models.There are two cases to illustrate how open source and international standards interact with each other.展开更多
High-resolution modeling approach is increasingly being considered as a necessary step for improving the monitoring and predictions of regional air quality. This is especially true for highly urbanized region with com...High-resolution modeling approach is increasingly being considered as a necessary step for improving the monitoring and predictions of regional air quality. This is especially true for highly urbanized region with complex terrain and land-use. This study uses Community Multiscale Air Quality （CMAQ） model coupled with MM5 mesoscale model for a comprehensive analysis to assess the suitability of such high-resolution modeling system in predicting ozone air quality in the complex terrains of Osaka, Japan. The 1-km and 3-kin grid domains were nested inside a 9-km domain and the domain with 1-km grid covered the Osaka region. High-resolution Grid Point Value-Mesoscale Model （GPV-MSM） data were used after suitable validation. The simulated ozone concentrations were validated and evaluated using statistical metrics using performance criteria set for ozone. Daily maxima of ozone were found better simulated by the 1-krn grid domain than the coarser 9-km and 3-km domains, with the maximum improvement in the mean absolute gross error about 3 ppbv. In addition, 1-km grid results fared better than other grids at most of the observation stations that showed noticeable differences in gross error as well as correlation. These results amply justify the use of the integrated high-resolution MM5-CMAQ modeling system in the highly urbanized region, such as the Osaka region, which has complex terrain and land-use.展开更多
BACKGROUND Gestational diabetes mellitus(GDM)is a condition characterized by high blood sugar levels during pregnancy.The prevalence of GDM is on the rise globally,and this trend is particularly evident in China,which...BACKGROUND Gestational diabetes mellitus(GDM)is a condition characterized by high blood sugar levels during pregnancy.The prevalence of GDM is on the rise globally,and this trend is particularly evident in China,which has emerged as a significant issue impacting the well-being of expectant mothers and their fetuses.Identifying and addressing GDM in a timely manner is crucial for maintaining the health of both expectant mothers and their developing fetuses.Therefore,this study aims to establish a risk prediction model for GDM and explore the effects of serum ferritin,blood glucose,and body mass index(BMI)on the occurrence of GDM.AIM To develop a risk prediction model to analyze factors leading to GDM,and evaluate its efficiency for early prevention.METHODS The clinical data of 406 pregnant women who underwent routine prenatal examination in Fujian Maternity and Child Health Hospital from April 2020 to December 2022 were retrospectively analyzed.According to whether GDM occurred,they were divided into two groups to analyze the related factors affecting GDM.Then,according to the weight of the relevant risk factors,the training set and the verification set were divided at a ratio of 7:3.Subsequently,a risk prediction model was established using logistic regression and random forest models,and the model was evaluated and verified.RESULTS Pre-pregnancy BMI,previous history of GDM or macrosomia,hypertension,hemoglobin(Hb)level,triglyceride level,family history of diabetes,serum ferritin,and fasting blood glucose levels during early pregnancy were determined.These factors were found to have a significant impact on the development of GDM(P<0.05).According to the nomogram model’s prediction of GDM in pregnancy,the area under the curve(AUC)was determined to be 0.883[95%confidence interval(CI):0.846-0.921],and the sensitivity and specificity were 74.1%and 87.6%,respectively.The top five variables in the random forest model for predicting the occurrence of GDM were serum ferritin,fasting blood glucose in early pregnancy,pre-pregnancy BMI,Hb level and triglyceride level.The random forest model achieved an AUC of 0.950(95%CI:0.927-0.973),the sensitivity was 84.8%,and the specificity was 91.4%.The Delong test showed that the AUC value of the random forest model was higher than that of the decision tree model(P<0.05).CONCLUSION The random forest model is superior to the nomogram model in predicting the risk of GDM.This method is helpful for early diagnosis and appropriate intervention of GDM.展开更多
The planetary boundary layer turbulence and moist convection parameterizations have been modified recently in the NASA Goddard Institute for Space Studies （GISS） Model E2 atmospheric general circulation model （GCM;...The planetary boundary layer turbulence and moist convection parameterizations have been modified recently in the NASA Goddard Institute for Space Studies （GISS） Model E2 atmospheric general circulation model （GCM; post-CMIP5, hereafter P5）. In this study, single column model （SCM_P5） simulated cloud fractions （CFs）, cloud liquid water paths （LWPs） and precipitation were compared with Atmospheric Radiation Measurement （ARM） Southern Great Plains （SGP） groundbased observations made during the period 2002-08. CMIP5 SCM simulations and GCM outputs over the ARM SGP region were also used in the comparison to identify whether the causes of cloud and precipitation biases resulted from either the physical parameterization or the dynamic scheme. The comparison showed that the CMIP5 SCM has difficulties in simulating the vertical structure and seasonal variation of low-level clouds. The new scheme implemented in the turbulence parameterization led to significantly improved cloud simulations in P5. It was found that the SCM is sensitive to the relaxation time scale. When the relaxation time increased from 3 to 24 h, SCM_P5-simulated CFs and LWPs showed a moderate increase （10%-20%） but precipitation increased significantly （56%）, which agreed better with observations despite the less accurate atmospheric state. Annual averages among the GCM and SCM simulations were almost the same, but their respective seasonal variations were out of phase. This suggests that the same physical cloud parameterization can generate similar statistical results over a long time period, but different dynamics drive the differences in seasonal variations. This study can potentially provide guidance for the further development of the GISS model.展开更多
The simulated Arctic sea ice drift and its relationship with the near-surface wind and surface ocean current during 1979-2014 in nine models from China that participated in the sixth phase of the Coupled Model Interco...The simulated Arctic sea ice drift and its relationship with the near-surface wind and surface ocean current during 1979-2014 in nine models from China that participated in the sixth phase of the Coupled Model Intercomparison Project(CMIP6)are examined by comparison with observational and reanalysis datasets.Most of the models reasonably represent the Beaufort Gyre(BG)and Transpolar Drift Stream(TDS)in the spatial patterns of their long-term mean sea ice drift,while the detailed location,extent,and strength of the BG and TDS vary among the models.About two-thirds of the models agree with the observation/reanalysis in the sense that the sea ice drift pattern is consistent with the near-surface wind pattern.About the same proportion of models shows that the sea ice drift pattern is consistent with the surface ocean current pattern.In the observation/reanalysis,however,the sea ice drift pattern does not match well with the surface ocean current pattern.All nine models missed the observational widespread sea ice drift speed acceleration across the Arctic.For the Arctic basin-wide spatial average,five of the nine models overestimate the Arctic long-term(1979-2014)mean sea ice drift speed in all months.Only FGOALS-g3 captures a significant sea ice drift speed increase from 1979 to 2014 both in spring and autumn.The increases are weaker than those in the observation.This evaluation helps assess the performance of the Arctic sea ice drift simulations in these CMIP6 models from China.展开更多
Background:Bladder cancer poses a great burden on society and its high rate of recurrence and treatment failure necessitates use of appropriate animal models to study its pathogenesis and test novel treatments.Orthoto...Background:Bladder cancer poses a great burden on society and its high rate of recurrence and treatment failure necessitates use of appropriate animal models to study its pathogenesis and test novel treatments.Orthotopic models are superior to other types since they provide a normal microenvironment.Four methods are described for developing bladder cancer models inside the animal’s bladder.Direct intramural injection is one of these methods and is widely used.However,its efficacy in model development has not yet been studied.We aimed to evaluate the efficacy and success rate of the direct intramural injection method of developing an orthotopic model for the study of bladder cancer.Method:Tumor cell lines were prepared in four microtubes.Aliquots of 200×10^(3) cells were injected through a 27 gauge needle into the ventral wall of the bladders of 4male and 4 female BALB/c mice following a midline 1 cm laparotomy incision.In addition,1 million cells from each microtube were injected into the flanks of control mice.To prevent infection and alleviate pain,5 mg/kg enrofloxacin and 2.5 mg/kg flunixin meglumine,respectively,were injected subcutaneously.Results:Tumors formed in all mice,resulting in 100% take rate and zero post-operation mortality.Surgery time was≤15 min per mouse.In two mice,tumors were found in the peritoneal space as well.Conclusion:Direct intramural injection is a rapid,reliable,and reproducible method for developing orthotopic models of bladder cancer.It can be done on both male and female mice and only requires readily available surgical tools.However,needle track can result in cell spillage and peritoneal tumors.展开更多
基金Science and Technology Research Project of Guangdong Meteorological Service(GRMC2021M19,GRMC2022Q16,GRMC2023M29)。
文摘The Pearl River Estuary(PRE)is one of China’s busiest shipping hubs and fishery production centers,as well as a region with abundant island tourism and wind energy resources,which calls for accurate short-term wind forecasts.First,this study evaluated three operational numerical models,i.e.,ECMWF-EC,NCEP-GFS,and CMA-GD,for their ability to predict short-term wind speed over the PRE against in-situ observations during 2018-2021.Overall,ECMWF-EC out-performs other models with an average RMSE of 2.24 m s^(-1)and R of 0.57,but the NCEP-GFS performs better in the case of strong winds.Then,various bias correction and multi-model ensemble(MME)methods are used to perform the deterministic post-processing using a local and lead-specific scheme.Two-factor model output statistics(MOS2)is the optimal bias correction method for reducing(increasing)the overall RMSE(R)to 1.62(0.70)m s^(-1),demonstrating the benefits of considering both initial and lead-specific information.Intercomparison of MME results reveals that Multiple linear regression(MLR)presents superior skills,followed by random forest(RF),but it is slightly inferior to MOS2,particularly for the first few forecasting hours.Furthermore,the incorporation of additional features in MLR reduces the overall RMSE to 1.53 m s^(-1)and increases R to 0.74.Similarly,RF presents comparable results,and both outperform MOS2 in terms of correcting their deficiencies at the first few lead hours and limiting the error growth rate.Despite the satisfactory skill of deterministic post-processing techniques,they are unable to achieve a balanced performance between mean and extreme statistics.This highlights the necessity for further development of probabilistic forecasts.
基金supported by the Large-scale Industry Model Evaluation Capability Development(CXFZ2024004)the National Social Science Foundation of China(22ZD035)the Research Innovation Project Plan of China University of Political Science and Law(24KYGH021).
文摘The purpose of this paper is to explore the application of large language models(LLMs)in legal case retrieval and to evaluate their potential for providing legal professionals with more efficient work aids.Currently,although pre-trained models have made great progress in legal case retrieval,they are often limited to specific types of law(e.g.,criminal law,civil law,etc.)and lack the ability to generalize across different types of law.Moreover,most models can only deal with a single task,whereas the legal case retrieval task requires a model to have a superb comprehension of legal texts,involving multiple subtasks and requiring multitasking capabilities.Therefore,the large language model,which has super generalization and multitasking ability,can solve the above problems.In order to explore the application of large language models for legal case retrieval in the legal domain,this paper evaluates a series of emerging large language models,including multilingual models,homegrown large models,and models specifically designed for the legal domain.These models are used to retrieve legal cases and its associated subtasks.Based on the Supreme People’s Court definition,the legal case retrieval task is broken down into seven subtasks:event detection,fact generation,trigger word extraction,keyword extraction,summarization,dispute focus identification,and reasoning generation.Using a variety of evaluation metrics,the experiments demonstrated that these emerging models have significant potential in the field of legal case retrieval,even with few shot samples.The research in this paper not only introduces new ideas in the field of legal case retrieval,but also empirically verifies the potential of LLMs to improve the quality and efficiency of retrieval.It proves the value of large language models in this field and is expected to significantly enhance the efficiency of legal practitioners,as well as promote the consistency and fairness of legal judgments through the use of emerging technologies.
文摘With the rapid development of generative artificial intelligence technologies,represented by large language models,university-level computer science education is undergoing a critical transition-from knowledge-based instruction to competency-oriented teaching.A postgraduate student competency evaluation model can serve as a framework to organize and guide both teaching and research activities at the postgraduate level.A number of relevant research efforts have already been conducted in this area.Graduate education plays a vital role not only as a continuation and enhancement of undergraduate education but also as essential preparation for future research endeavors.An analysis of the acceptance of competency evaluation models refers to the assessment of how various stakeholders perceive the importance of different components within the model.Investigating the degree of acceptance among diverse groups-such as current undergraduate students,current postgraduate students,graduates with less than three years of work experience,and those with more than three years of work experience-can offer valuable insights for improving and optimizing postgraduate education and training practices.
文摘With the continuous development of the nursing discipline,standardized nurse training has always been a crucial link in the development of nursing science and plays an irreplaceable role in talent cultivation.However,in the current standardized training for some nurses,there are problems such as the simplification of nursing skill evaluation models and insufficient post competence of nurses.Therefore,optimizing the training model for nursing talents has become an inevitable measure.The problem-based learning(PBL)method and the Direct Observation of Procedural Skills(DOPS)evaluation model provide new directions and guidance for the development of training.Against this background,this paper explores effective approaches for standardized nurse training,starting from basic concepts and gradually delving into specific practical paths,aiming to improve the quality of talent cultivation and provide valuable references for other researchers.
文摘Objective:To explore the application value of a new empowerment teaching method based on Kirkpatrick’s evaluation model in teaching Chinese medicine nursing in otorhinolaryngology.Methods:60 nurses who practiced in the otolaryngology department of our hospital from June 2022 to October 2024 were included in the study and equally divided into two groups using a convenient sampling method.30 nurses who chose traditional Chinese medicine skill teaching management were included in the control group,and 30 nurses who chose the new empowerment teaching method based on Kirkpatrick’s evaluation model were included in the observation group.Relevant indicators such as clinical teaching environment perception,theoretical knowledge scores of Chinese medicine nursing,and excellent rate of practical operation assessment were compared.Results:The nurses in the observation group had higher scores for clinical teaching environment perception than the control group(P<0.05).However,the midterm and final exam scores for theoretical knowledge of Chinese medicine nursing were higher in the observation group than in the control group(P<0.05).Compared with the control group,the observation group had a higher excellent rate of practical operation assessment(93.33%>73.33%)and a higher Chinese medicine nursing ability score[(215.69±19.73)points>(184.87±15.66)points](P<0.05).Conclusion:Applying the new empowerment teaching method based on Kirkpatrick’s evaluation model to Chinese medicine nursing teaching in otolaryngology can help nurses understand the theoretical knowledge of Chinese medicine nursing and optimize the clinical teaching environment,thereby promoting their practical skills and Chinese medicine nursing abilities.
基金supported in part by the Education Reform Key Projects of Heilongjiang Province(Grant No.SJGZ20220011,SJGZ20220012)the Excellent Project of Ministry of Education and China Higher Education Association on Digital Ideological and Political Education in Universities(Grant No.GXSZSZJPXM001)。
文摘This paper proposes a multivariate data fusion based quality evaluation model for software talent cultivation.The model constructs a comprehensive ability and quality evaluation index system for college students from a perspective of engineering course,especially of software engineering.As for evaluation method,relying on the behavioral data of students during their school years,we aim to construct the evaluation model as objective as possible,effectively weakening the negative impact of personal subjective assumptions on the evaluation results.
基金primarily supported by the National Key R&D Program of China[grant number 2021YFC3000904]the Jiangsu Provincial Key Technology R&D Program[grant number BE2022851]National Natural Science Foundation of China[grant number 42405035]。
文摘Vector winds play a crucial role in weather and climate,as well as the effective utilization of wind energy resources.However,limited research has been conducted on treating the wind field as a vector field in the evaluation of numerical weather prediction models.In this study,the authors treat vector winds as a whole by employing a vector field evaluation method,and evaluate the mesoscale model of the China Meteorological Administration(CMA-MESO)and ECMWF forecast,with reference to ERA5 reanalysis,in terms of multiple aspects of vector winds over eastern China in 2022.The results show that the ECMWF forecast is superior to CMA-MESO in predicting the spatial distribution and intensity of 10-m vector winds.Both models overestimate the wind speed in East China,and CMA-MESO overestimates the wind speed to a greater extent.The forecasting skill of the vector wind field in both models decreases with increasing lead time.The forecasting skill of CMA-MESO fluctuates more and decreases faster than that of the ECMWF forecast.There is a significant negative correlation between the model vector wind forecasting skill and terrain height.This study provides a scientific evaluation of the local application of vector wind forecasts of the CMA-MESO model and ECMWF forecast.
基金supported by the National Natural Science Foundation of China[grant number 42175132]the National Key R&D Program[grant number 2020YFA0607802]the CAS Information Technology Program[grant number CAS-WX2021SF-0107-02]。
文摘Solar energy is a pivotal clean energy source in the transition to carbon neutrality from fossil fuels.However,the intermittent and stochastic characteristics of solar radiation pose challenges for accurate simulation and prediction.Accurately simulating and predicting solar radiation and its variability are crucial for optimizing solar energy utilization.This study conducted simulation experiments using the WRF-Solar model from 25 June to 25 July 2022,to evaluate the accuracy and performance of the simulated solar radiation across China.The simulations covered the whole country with a grid spacing of 27 km and were compared with ground observation network data from the Chinese Ecosystem Research Network.The results indicated that WRF-Solar can accurately capture the spatiotemporal patterns of global horizontal irradiance over China,but there is still an overestimation of solar radiation,and the model underestimates the total cloud cover.The root-mean-square error ranged from 92.83 to 188.13 W m^(-2) and the mean bias(MB)ranged from 21.05 to 56.22 W m^(-2).The simulation showed the smallest MB at Lhasa on the Qinghai–Tibet Plateau,while the largest MB was observed in Southeast China.To enhance the accuracy of solar radiation simulation,the authors compared the Fast All-sky Radiation Model for Solar with the Rapid Radiative Transfer Model for General Circulation Models and found that the former provides better simulation.
基金funded by the National Natural Science Foundation of China(Grant Nos.U21A6001,42261144687,42175173)the Project supported by Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)(Grant No.SML2023SP208)the GuangDong Basic and Applied Basic Research Foundation(2023A1515240036).
文摘Based on the C-Coupler platform,the semi-unstructured Climate System Model,Synthesis Community Integrated Model version 2(SYCIM2.0),has been developed at the School of Atmospheric Sciences,Sun Yat-sen University.SYCIM2.0 aims to meet the demand for seamless climate prediction through accurate climate simulations and projections.This paper provides an overview of SYCIM2.0 and highlights its key features,especially the coupling of an unstructured ocean model and the tuning process.An extensive evaluation of its performance,focusing on the East Asian Summer Monsoon(EASM),is presented based on long-term simulations with fixed external forcing.The results suggest that after nearly 240 years of integration,SYCIM2.0 achieves a quasi-equilibrium state,albeit with small trends in the net radiation flux at the top-of-atmosphere(TOA)and Earth’s surface,as well as with global mean near-surface temperatures.Compared to observational and reanalysis data,the model realistically simulates spatial patterns of sea surface temperature(SST)and precipitation centers to include their annual cycles,in addition to the lower-level wind fields in the EASM region.However,it exhibits a weakened and eastward-shifted Western Pacific Subtropical High(WPSH),resulting in an associated precipitation bias.SYCIM2.0 robustly captures the dominant mode of the EASM and its close relationship with the El Niño-Southern Oscillation(ENSO)but exhibits relatively poor performance in simulating the second leading mode and the associated air–sea interaction processes.Further comprehensive evaluations of SYCIM2.0 will be conducted in future studies.
基金funded by the Natural Science Foundation of China(Grant No.W2412145,42275031)the Natural Science Foundation of Yunnan Province(Grant No.202302AN360006)。
文摘Snow cover over the Tibetan Plateau(TP)(TPSC)has garnered significant attention as a crucial indicator of climate change,along with its variations and related climate processes.However,due to the complex terrain of the TP,most numerical models exhibit notable uncertainty in simulating snow conditions in this area.This study evaluates historical snow simulations and related climate anomalies over the TP in numerical models from phase 6 of the Coupled Model Intercomparison Project(CMIP6).The CMIP6 model simulations are compared with two observation-based products across different seasons and temporal scales,and the results indicate that the CMIP6 multimodel ensemble(MME)mean reasonably captures the spatial distribution of the annual and seasonal climatological mean TP snow,albeit with weaker magnitudes compared to observations.The CMIP6 MME performs better over the western TP than the eastern regions,showing a higher reproducibility of the long-term warming trends and declining snow cover trends,partly due to the atmospheric circulation anomalies related to global warming.Additionally,some CMIP6 models successfully capture the interannual variability of TPSC and its relationship with associated climate factors.Our work emphasizes the importance of CMIP6 model selection and pays attention to data reliability in interpreting CMIP6 model results across different TP regions when studying snow cover variations and climate effects using numerical models.
基金Supported by the Laoshan Laboratory(No.LSKJ 202202404)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB 42000000)+1 种基金the National Natural Science Foundation of China(NSFC)(No.42030410)the Startup Foundation for Introducing Talent of NUIST,and the Jiangsu Innovation Research Group(No.JSSCTD 202346)。
文摘Numerical models are crucial for quantifying the ocean-atmosphere interactions associated with the El Niño-Southern Oscillation(ENSO)phenomenon in the tropical Pacific.Current coupled models often exhibit significant biases and inter-model differences in simulating ENSO,underscoring the need for alternative modeling approaches.The Regional Ocean Modeling System(ROMS)is a sophisticated ocean model widely used for regional studies and has been coupled with various atmospheric models.However,its application in simulating ENSO processes on a basin scale in the tropical Pacific has not been explored.For the first time,this study presents the development of a basin-scale hybrid coupled model(HCM)for the tropical Pacific,integrating ROMS with a statistical atmospheric model that captures the interannual relationships between sea surface temperature(SST)and wind stress anomalies.The HCM is evaluated for its capability to simulate the annual mean,seasonal,and interannual variations of the oceanic state in the tropical Pacific.Results demonstrate that the model effectively reproduces the ENSO cycle,with a dominant oscillation period of approximately two years.The ROMS-based HCM developed here offers an efficient and robust tool for investigating climate variability in the tropical Pacific.
文摘An internal defect meter is an instrument to detect the internal inclusion defects of cold-rolled strip steel.The detection accuracy of the equipment can be evaluated based on the similarity of the multiple detection data obtained for the same steel coil.Based on the cosine similarity model and eigenvalue matrix model,a comprehensive evaluation method to calculate the weighted average of similarity is proposed.Results show that the new method is consistent with and can even replace artificial evaluation to realize the automatic evaluation of strip defect detection results.
基金supported by the National Natural Science Foundation of China (Grant No.42377143)Sichuan Natural Science Foundation (Grant No.2024NSFSC0097)the Open Fund of State Key Laboratory of Coal Mining and Clean Utilization,China (Grant No.2021-CMCU-KFZD001).
文摘In the concurrent extraction of coal and gas,the quantitative assessment of evolving characteristics in mining-induced fracture networks and mining-enhanced permeability within coal seams serves as the cornerstone for effective gas extraction.However,representing mining-induced fracture networks from a three-dimensional(3D)sight and developing a comprehensive model to evaluate the anisotropic mining-enhanced permeability characteristics still pose significant challenges.In this investigation,a field experiment was undertaken to systematically monitor the evolution of borehole fractures in the coal mass ahead of the mining face at the Pingdingshan Coal Mining Group in China.Using the testing data of borehole fracture,the mining-induced fracture network at varying distances from the mining face was reconstructed through a statistical reconstruction method.Additionally,utilizing fractal theory,a model for the permeability enhancement rate(PER)induced by mining was established.This model was employed to quantitatively depict the anisotropic evolution patterns of PER as the mining face advanced.The research conclusions are as follows:(1)The progression of the mining-induced fracture network can be classified into the stage of rapid growth,the stage of stable growth,and the stage of weak impact;(2)The PER of mining-induced fracture network exhibited a typical progression that can be characterized with slow growth,rapid growth and significant decline;(3)The anisotropic mining-enhanced permeability of the reconstructed mining-induced fracture networks were significant.The peak PER in the vertical direction of the coal seam is 6.86 times and 4446.38 times greater than the direction perpendicular to the vertical thickness and the direction parallel to the advancement of the mining face,respectively.This investigatione provides a viable approach and methodology for quantitatively assessing the anisotropic PER of fracture networks induced during mining,in the concurrent exploitation of coal and gas.
基金Sponsored by Provincial-level Undergraduate Innovation Training Program of Anhui Xinhua University(S202312216043)Natural Science Key Research Program for Colleges and Universities in Anhui Province(2023AH051816)Anhui General Teaching Research Project(2022jyxm665).
文摘Through the investigation of the species of street trees located along the main urban roads in Hefei City,a total of 22 species were selected,belonging to 16 families and 22 genera,with the Sapindaceae family being the most prevalent.In this study,the Analytic Hierarchy Process(AHP)was employed to assess the comprehensive value of 22 species of street trees applied along the main urban roads in Hefei City.Fourteen evaluation criteria were selected from four categories:morphological indices,functional indices,resistance indices,and management indices,to develop a comprehensive evaluation model.Based on a composite score derived from 22 street trees,these trees were classified into three distinct grades.Grade I(L≥3.0)exhibited a high comprehensive application value in Hefei City and included 6 tree species,such asPlatanus.Grade II(2.5≤L<3.0)also demonstrated a high comprehensive application value,comprising 15 tree species,includingCatalpabungei.In contrast,grade III(L<2.5)indicated a general comprehensive application value,represented by a single species,Cedrusdeodara.The evaluation results can offer theoretical insights for the selection of urban street trees.
文摘In the era of AI,especially large models,the importance of open source has become increasingly prominent.First,open source allows innovation to avoid starting from scratch.Through iterative innovation,it promotes technical exchanges and learning globally.Second,resources required for large model R&D are difficult for a single institution to obtain.The evaluation of general large models also requires the participation of experts from various industries.Third,without open source collaboration,it is difficult to form a unified upper-layer software ecosystem.Therefore,open source has become an important cooperation mechanism to promote the development of AI and large models.There are two cases to illustrate how open source and international standards interact with each other.
文摘High-resolution modeling approach is increasingly being considered as a necessary step for improving the monitoring and predictions of regional air quality. This is especially true for highly urbanized region with complex terrain and land-use. This study uses Community Multiscale Air Quality （CMAQ） model coupled with MM5 mesoscale model for a comprehensive analysis to assess the suitability of such high-resolution modeling system in predicting ozone air quality in the complex terrains of Osaka, Japan. The 1-km and 3-kin grid domains were nested inside a 9-km domain and the domain with 1-km grid covered the Osaka region. High-resolution Grid Point Value-Mesoscale Model （GPV-MSM） data were used after suitable validation. The simulated ozone concentrations were validated and evaluated using statistical metrics using performance criteria set for ozone. Daily maxima of ozone were found better simulated by the 1-krn grid domain than the coarser 9-km and 3-km domains, with the maximum improvement in the mean absolute gross error about 3 ppbv. In addition, 1-km grid results fared better than other grids at most of the observation stations that showed noticeable differences in gross error as well as correlation. These results amply justify the use of the integrated high-resolution MM5-CMAQ modeling system in the highly urbanized region, such as the Osaka region, which has complex terrain and land-use.
文摘BACKGROUND Gestational diabetes mellitus(GDM)is a condition characterized by high blood sugar levels during pregnancy.The prevalence of GDM is on the rise globally,and this trend is particularly evident in China,which has emerged as a significant issue impacting the well-being of expectant mothers and their fetuses.Identifying and addressing GDM in a timely manner is crucial for maintaining the health of both expectant mothers and their developing fetuses.Therefore,this study aims to establish a risk prediction model for GDM and explore the effects of serum ferritin,blood glucose,and body mass index(BMI)on the occurrence of GDM.AIM To develop a risk prediction model to analyze factors leading to GDM,and evaluate its efficiency for early prevention.METHODS The clinical data of 406 pregnant women who underwent routine prenatal examination in Fujian Maternity and Child Health Hospital from April 2020 to December 2022 were retrospectively analyzed.According to whether GDM occurred,they were divided into two groups to analyze the related factors affecting GDM.Then,according to the weight of the relevant risk factors,the training set and the verification set were divided at a ratio of 7:3.Subsequently,a risk prediction model was established using logistic regression and random forest models,and the model was evaluated and verified.RESULTS Pre-pregnancy BMI,previous history of GDM or macrosomia,hypertension,hemoglobin(Hb)level,triglyceride level,family history of diabetes,serum ferritin,and fasting blood glucose levels during early pregnancy were determined.These factors were found to have a significant impact on the development of GDM(P<0.05).According to the nomogram model’s prediction of GDM in pregnancy,the area under the curve(AUC)was determined to be 0.883[95%confidence interval(CI):0.846-0.921],and the sensitivity and specificity were 74.1%and 87.6%,respectively.The top five variables in the random forest model for predicting the occurrence of GDM were serum ferritin,fasting blood glucose in early pregnancy,pre-pregnancy BMI,Hb level and triglyceride level.The random forest model achieved an AUC of 0.950(95%CI:0.927-0.973),the sensitivity was 84.8%,and the specificity was 91.4%.The Delong test showed that the AUC value of the random forest model was higher than that of the decision tree model(P<0.05).CONCLUSION The random forest model is superior to the nomogram model in predicting the risk of GDM.This method is helpful for early diagnosis and appropriate intervention of GDM.
基金supported by the DOE ASR program(Grant No.DESC008468)
文摘The planetary boundary layer turbulence and moist convection parameterizations have been modified recently in the NASA Goddard Institute for Space Studies （GISS） Model E2 atmospheric general circulation model （GCM; post-CMIP5, hereafter P5）. In this study, single column model （SCM_P5） simulated cloud fractions （CFs）, cloud liquid water paths （LWPs） and precipitation were compared with Atmospheric Radiation Measurement （ARM） Southern Great Plains （SGP） groundbased observations made during the period 2002-08. CMIP5 SCM simulations and GCM outputs over the ARM SGP region were also used in the comparison to identify whether the causes of cloud and precipitation biases resulted from either the physical parameterization or the dynamic scheme. The comparison showed that the CMIP5 SCM has difficulties in simulating the vertical structure and seasonal variation of low-level clouds. The new scheme implemented in the turbulence parameterization led to significantly improved cloud simulations in P5. It was found that the SCM is sensitive to the relaxation time scale. When the relaxation time increased from 3 to 24 h, SCM_P5-simulated CFs and LWPs showed a moderate increase （10%-20%） but precipitation increased significantly （56%）, which agreed better with observations despite the less accurate atmospheric state. Annual averages among the GCM and SCM simulations were almost the same, but their respective seasonal variations were out of phase. This suggests that the same physical cloud parameterization can generate similar statistical results over a long time period, but different dynamics drive the differences in seasonal variations. This study can potentially provide guidance for the further development of the GISS model.
基金supported by the National Key R&D Program of China(Grant No.2018YFA0605904)the National Natural Science Foundation of China(Grant No.41701411).
文摘The simulated Arctic sea ice drift and its relationship with the near-surface wind and surface ocean current during 1979-2014 in nine models from China that participated in the sixth phase of the Coupled Model Intercomparison Project(CMIP6)are examined by comparison with observational and reanalysis datasets.Most of the models reasonably represent the Beaufort Gyre(BG)and Transpolar Drift Stream(TDS)in the spatial patterns of their long-term mean sea ice drift,while the detailed location,extent,and strength of the BG and TDS vary among the models.About two-thirds of the models agree with the observation/reanalysis in the sense that the sea ice drift pattern is consistent with the near-surface wind pattern.About the same proportion of models shows that the sea ice drift pattern is consistent with the surface ocean current pattern.In the observation/reanalysis,however,the sea ice drift pattern does not match well with the surface ocean current pattern.All nine models missed the observational widespread sea ice drift speed acceleration across the Arctic.For the Arctic basin-wide spatial average,five of the nine models overestimate the Arctic long-term(1979-2014)mean sea ice drift speed in all months.Only FGOALS-g3 captures a significant sea ice drift speed increase from 1979 to 2014 both in spring and autumn.The increases are weaker than those in the observation.This evaluation helps assess the performance of the Arctic sea ice drift simulations in these CMIP6 models from China.
基金Tehran University of Medical Sciences and Health ServicesGrant/Award Number:98-3-101-45499。
文摘Background:Bladder cancer poses a great burden on society and its high rate of recurrence and treatment failure necessitates use of appropriate animal models to study its pathogenesis and test novel treatments.Orthotopic models are superior to other types since they provide a normal microenvironment.Four methods are described for developing bladder cancer models inside the animal’s bladder.Direct intramural injection is one of these methods and is widely used.However,its efficacy in model development has not yet been studied.We aimed to evaluate the efficacy and success rate of the direct intramural injection method of developing an orthotopic model for the study of bladder cancer.Method:Tumor cell lines were prepared in four microtubes.Aliquots of 200×10^(3) cells were injected through a 27 gauge needle into the ventral wall of the bladders of 4male and 4 female BALB/c mice following a midline 1 cm laparotomy incision.In addition,1 million cells from each microtube were injected into the flanks of control mice.To prevent infection and alleviate pain,5 mg/kg enrofloxacin and 2.5 mg/kg flunixin meglumine,respectively,were injected subcutaneously.Results:Tumors formed in all mice,resulting in 100% take rate and zero post-operation mortality.Surgery time was≤15 min per mouse.In two mice,tumors were found in the peritoneal space as well.Conclusion:Direct intramural injection is a rapid,reliable,and reproducible method for developing orthotopic models of bladder cancer.It can be done on both male and female mice and only requires readily available surgical tools.However,needle track can result in cell spillage and peritoneal tumors.
