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Identification of Myosinon the Surface of Wheat Mitochondria
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作者 赵和平 刘爱校 +2 位作者 任东涛 刘国琴 阎隆飞 《Acta Botanica Sinica》 CSCD 1999年第12期1303-1306,共4页
Itwasdemonstratedthatmyosinwasassociatedwiththesurfaceofmitochondriainwheat (Triticum aestivumL .) .AssaysofpolyacrylamidegelelectrophoresisandWesternblottinghaveshownthatapolypeptide withmolecularweightof2 10kDcoul... Itwasdemonstratedthatmyosinwasassociatedwiththesurfaceofmitochondriainwheat (Triticum aestivumL .) .AssaysofpolyacrylamidegelelectrophoresisandWesternblottinghaveshownthatapolypeptide withmolecularweightof2 10kDcouldberecognizedbyapolyclonalantibodyagainsthumanmusclemyosin .It wasfoundthattheATPaseactivityofmitochondrialsuspensioncouldbestimulatedbyF_actinisolatedfrom chickenmuscle ,whichindicatedthattherewasmyosinonthesurfaceofwheatmitochondria .Thisresultwas confirmedbyelectronmicroscopicobservation :mitochondriatreatedwithN_ethylmaleimide (NEM)couldbe wrappedbytheF_actin . 展开更多
关键词 mitochondriainwheat MYOSIN F_actin ATPASE LOCALIZATION
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