植物线粒体具有产生能量、氧化还原调控、代谢物合成、逆境和病害应答等重要功能,对于植物生存至关重要。由植物线粒体DNA序列产生的细胞质雄性不育(cytoplasmic male sterility,CMS)在植物杂交育种中具有重要的应用价值。但由于缺乏线...植物线粒体具有产生能量、氧化还原调控、代谢物合成、逆境和病害应答等重要功能,对于植物生存至关重要。由植物线粒体DNA序列产生的细胞质雄性不育(cytoplasmic male sterility,CMS)在植物杂交育种中具有重要的应用价值。但由于缺乏线粒体遗传操作技术手段,大多数作物CMS基因功能都未能进行验证,线粒体遗传过程及其调控CMS发生的分子机理仍未能被清楚阐明。本文综述了近年提出的基于tRNA输送、PPR蛋白重新设计和mitoTALEN三种植物线粒体遗传操作技术,分别对其技术原理及其应用进行了介绍,为将来研究线粒体基因功能提供参考依据。展开更多
Neuronal death is associated with mitochondrial dysfunction caused by mutations in mitochondrial DNA.Mitochondrial DNA becomes damaged when processes such as replica-tion,repair,and nucleotide synthesis are compromise...Neuronal death is associated with mitochondrial dysfunction caused by mutations in mitochondrial DNA.Mitochondrial DNA becomes damaged when processes such as replica-tion,repair,and nucleotide synthesis are compromised.This extensive accumulation of damaged mitochondrial DNA subsequently disrupts the normal function of mitochondria,lead-ing to aging,degeneration,or even death of neurons.Mitochondrial dysfunction stands as a pivotal factor in the development of neurodegenerative diseases,including Parkinson’s dis-ease,Alzheimer’s disease,Huntington’s disease,and amyotrophic lateral sclerosis.Recog-nizing the intricate nature of their pathogenesis,there is an urgent need for more effective therapeutic interventions.In recent years,mitochondrial DNA editing tools such as zinc finger nucleases,double-stranded DNA deaminase toxin A-derived cytosine base editors,and tran-scription activator-like effector ligand deaminases have emerged.Their emergence will revo-lutionize the research and treatment of mitochondrial diseases.In this review,we summarize the advancements in mitochondrial base editing technology and anticipate its utilization in neurodegenerative diseases,offering insights that may inform preventive strategies and ther-apeutic interventions for disease phenotypes.展开更多
文摘植物线粒体具有产生能量、氧化还原调控、代谢物合成、逆境和病害应答等重要功能,对于植物生存至关重要。由植物线粒体DNA序列产生的细胞质雄性不育(cytoplasmic male sterility,CMS)在植物杂交育种中具有重要的应用价值。但由于缺乏线粒体遗传操作技术手段,大多数作物CMS基因功能都未能进行验证,线粒体遗传过程及其调控CMS发生的分子机理仍未能被清楚阐明。本文综述了近年提出的基于tRNA输送、PPR蛋白重新设计和mitoTALEN三种植物线粒体遗传操作技术,分别对其技术原理及其应用进行了介绍,为将来研究线粒体基因功能提供参考依据。
文摘Neuronal death is associated with mitochondrial dysfunction caused by mutations in mitochondrial DNA.Mitochondrial DNA becomes damaged when processes such as replica-tion,repair,and nucleotide synthesis are compromised.This extensive accumulation of damaged mitochondrial DNA subsequently disrupts the normal function of mitochondria,lead-ing to aging,degeneration,or even death of neurons.Mitochondrial dysfunction stands as a pivotal factor in the development of neurodegenerative diseases,including Parkinson’s dis-ease,Alzheimer’s disease,Huntington’s disease,and amyotrophic lateral sclerosis.Recog-nizing the intricate nature of their pathogenesis,there is an urgent need for more effective therapeutic interventions.In recent years,mitochondrial DNA editing tools such as zinc finger nucleases,double-stranded DNA deaminase toxin A-derived cytosine base editors,and tran-scription activator-like effector ligand deaminases have emerged.Their emergence will revo-lutionize the research and treatment of mitochondrial diseases.In this review,we summarize the advancements in mitochondrial base editing technology and anticipate its utilization in neurodegenerative diseases,offering insights that may inform preventive strategies and ther-apeutic interventions for disease phenotypes.
