The potential endocrine disrupting effects and other toxicity effects on aquatic biota resulted from food uptake was simulated by feeding the laboratory cultured rare minnow(Gobiocypris rarus) with field collected Lim...The potential endocrine disrupting effects and other toxicity effects on aquatic biota resulted from food uptake was simulated by feeding the laboratory cultured rare minnow(Gobiocypris rarus) with field collected Limnodrilus sp. The results indicated that the food chain processes affected significantly the growth, slightly reduced gonadosomatic indices, and elevated hepatosomatic indices. There was an obvious vitellogenin(VTG) induction, which generally only occurred in mature female, in the serum of juvenile rare minnow and mature male when fed with Limnodrilus sp. In addition, the rare minnow feeding on Limnodrilus sp. had significantly high renal indices, it meant obvious renal hyperplasia. The present work suggested that Limnodrilus sp. from field water may contain toxic pollutants and could lead to endocrine disruption effects to the predators. It was concluded that endocrine disruptors may not only be assimilated through water, but also be bioconcentrated through food web. The results also suggested the importance of food selection in conducting the study of endocrine disruption effects using sensitive species.展开更多
An 8-week feeding trial was conducted to detect the optimal dietary protein and energy, as well as the ef fects of protein to energy ratio on growth, for the rare minnow( Gobiocypris rarus), which are critical to nutr...An 8-week feeding trial was conducted to detect the optimal dietary protein and energy, as well as the ef fects of protein to energy ratio on growth, for the rare minnow( Gobiocypris rarus), which are critical to nutrition standardization for model fi sh. Twenty-four diets were formulated to contain three gross energy(10, 12.5, 15 kJ/g), four protein(20%, 25%, 30%, 35%), and two lipid levels(3%, 6%). The results showed that optimal dietary E/P was 41.7–50 kJ/g for maximum growth in juvenile rare minnows at 6% dietary crude lipid. At 3% dietary lipid, specifi c growth rate(SGR) increased markedly when E/P decreased from 62.5 kJ/g to 35.7 kJ/g and gross energy was 12.5 kJ/g, and from 75 kJ/g to 42.9 kJ/g when gross energy was 15.0 kJ/g. The optimal gross energy was estimated at 12.5 kJ /g and excess energy decreased food intake and growth. Dietary lipid exhibited an apparent protein-sparing eff ect. Optimal protein decreased from 35% to 25%–30% with an increase in dietary lipid from 3% to 6% without adversely ef fecting growth. Dietary lipid level af fects the optimal dietary E/P ratio. In conclusion, recommended dietary protein and energy for rare minnow are 20%–35% and 10–12.5 k J/g, respectively.展开更多
Adjustments to rearing practices should be justified with increases in production, stocking success, or angler satisfaction. Largemouth bass (<span style="white-space:nowrap;"><i></span>Mic...Adjustments to rearing practices should be justified with increases in production, stocking success, or angler satisfaction. Largemouth bass (<span style="white-space:nowrap;"><i></span>Micropterus salmoides<span style="white-space:nowrap;"></i></span>) production was assessed between hatchery ponds where fish were restricted to an invertebrate diet or received supplemental fathead minnow (<span style="white-space:nowrap;"><i></span>Pimephales promelas<span style="white-space:nowrap;"></i></span>) forage during 2015. At harvest, age-0 bass yield was 4.5 times greater and average fish length was 38 mm longer, in the pond that received fathead minnow. In 2016, a second study evaluated the timing of minnow supplementation that included earlier stockings of small fathead minnow (<30 mm) compared to delayed supplementation with larger (>30 mm) minnows. With earlier supplementation, bass yield was 2.3 times greater and fish averaged 14 mm longer at harvest. Bass survival was approximately 38% higher during 2015 when supplementation occurred and 25% higher during 2016 when minnow supplementation began earlier. Our findings show invertebrate forage was probably limiting bass production in hatchery ponds and supplementing with appropriately-sized fathead minnows increased age-0, largemouth bass production.展开更多
Based on the simulative exposure experiments of nitrobenzene to two small experimental fishes, Medaka (Oryzias latipes) and Chinese Rare Minnow (Gobiocypris rarus), it was found that nitrobenzene could decrease in a l...Based on the simulative exposure experiments of nitrobenzene to two small experimental fishes, Medaka (Oryzias latipes) and Chinese Rare Minnow (Gobiocypris rarus), it was found that nitrobenzene could decrease in a linear way in the static aquatic system. Both fishes could accumulate dose-related levels of nitrobenzene quickly and eliminate the compound rapidly when they were transferred to clean water. The Chinese rare minnow showed more sensitivity to the acute toxicity exposure of nitrobenzene than Medaka. Typical molecular biomarkers in oxidative defense system including superoxide dismu- tase (SOD), catalase (CAT) showed the chemical induced alterations. The changes of acetylcholi- nesterase (AChE) activities indicated that nitrobenzene might affect the normal neural function. Nitro- benzene exposure could also lead to obvious damaging effects on the target organs, such as gill and liver by hitopathological studies.展开更多
The acute (12 h) and chronic (7 or 14 d) effects of tributyltin chloride (TBT) on a new type of test fish, the Chinese rare minnow (Gobiocypris rarus), have been studied. High accumula-tion of the pollutant in the fis...The acute (12 h) and chronic (7 or 14 d) effects of tributyltin chloride (TBT) on a new type of test fish, the Chinese rare minnow (Gobiocypris rarus), have been studied. High accumula-tion of the pollutant in the fish muscle and obvious changes in hepatosomatic index and gonad somatic index were observed after exposure. The ultrastructural findings show a series of patho-logical changes existing in the exposed gill cells and hepatocytes, which demonstrates the toxicity effects of tributyltin compounds on aquatic organisms. The results clearly show that the Chinese rare minnow is a new sensitive test fish material, whose exposure system is suitable for the exten-sive organometallic toxicity studies.展开更多
目的和方法稀有鮈鲫(Gobiocypris rarus)作为一种本土特有的小型鱼类,是我国正在标准化的化学品毒性测试生物之一。为评价稀有鮈鲫鱼类急性毒性实验中效应的稳定性和可重复性,研究优化筛选了重铬酸钾和3,4-二氯苯胺两种化学品,分别在单...目的和方法稀有鮈鲫(Gobiocypris rarus)作为一种本土特有的小型鱼类,是我国正在标准化的化学品毒性测试生物之一。为评价稀有鮈鲫鱼类急性毒性实验中效应的稳定性和可重复性,研究优化筛选了重铬酸钾和3,4-二氯苯胺两种化学品,分别在单一实验室内及不同实验室间进行急性毒性验证实验。结果对于来源相同、体长固定的稀有鮈鲫,无论在实验室内还是实验室间,两组化学品的96 h LC50值均在x珋±2s的质量控制范围之内,所有结果体现了很好的稳定性和可重复性。结论根据研究获得的数据可建立一个有效的稳定性和可重复性衡量数据库,用来评估之后其他单个实验的可靠性;稀有鮈鲫作为一种具有潜力的生态毒性测试的生物种类,具有成为标准实验动物的潜能。展开更多
我国本土水生生物稀有鮈鲫(Gobiocypris rarus Ye et Fu,1983年)是一种优良的试验用鱼,具备成为模式生物必要条件。本文总结了稀有鮈鲫生物生态学研究情况,分析了稀有鮈鲫在多个应用领域与模式生物斑马鱼、青鳉存在的不足和差距,同时借...我国本土水生生物稀有鮈鲫(Gobiocypris rarus Ye et Fu,1983年)是一种优良的试验用鱼,具备成为模式生物必要条件。本文总结了稀有鮈鲫生物生态学研究情况,分析了稀有鮈鲫在多个应用领域与模式生物斑马鱼、青鳉存在的不足和差距,同时借鉴发达国家推动模式生物的实践经验,提出了针对性建议。展开更多
文摘The potential endocrine disrupting effects and other toxicity effects on aquatic biota resulted from food uptake was simulated by feeding the laboratory cultured rare minnow(Gobiocypris rarus) with field collected Limnodrilus sp. The results indicated that the food chain processes affected significantly the growth, slightly reduced gonadosomatic indices, and elevated hepatosomatic indices. There was an obvious vitellogenin(VTG) induction, which generally only occurred in mature female, in the serum of juvenile rare minnow and mature male when fed with Limnodrilus sp. In addition, the rare minnow feeding on Limnodrilus sp. had significantly high renal indices, it meant obvious renal hyperplasia. The present work suggested that Limnodrilus sp. from field water may contain toxic pollutants and could lead to endocrine disruption effects to the predators. It was concluded that endocrine disruptors may not only be assimilated through water, but also be bioconcentrated through food web. The results also suggested the importance of food selection in conducting the study of endocrine disruption effects using sensitive species.
基金Supported by the National Key Technology R&D Program of China(No.2011BAI15B01-41)the National High Technology Research and Development Program of China(863 Program)(No.2012AA06A302)
文摘An 8-week feeding trial was conducted to detect the optimal dietary protein and energy, as well as the ef fects of protein to energy ratio on growth, for the rare minnow( Gobiocypris rarus), which are critical to nutrition standardization for model fi sh. Twenty-four diets were formulated to contain three gross energy(10, 12.5, 15 kJ/g), four protein(20%, 25%, 30%, 35%), and two lipid levels(3%, 6%). The results showed that optimal dietary E/P was 41.7–50 kJ/g for maximum growth in juvenile rare minnows at 6% dietary crude lipid. At 3% dietary lipid, specifi c growth rate(SGR) increased markedly when E/P decreased from 62.5 kJ/g to 35.7 kJ/g and gross energy was 12.5 kJ/g, and from 75 kJ/g to 42.9 kJ/g when gross energy was 15.0 kJ/g. The optimal gross energy was estimated at 12.5 kJ /g and excess energy decreased food intake and growth. Dietary lipid exhibited an apparent protein-sparing eff ect. Optimal protein decreased from 35% to 25%–30% with an increase in dietary lipid from 3% to 6% without adversely ef fecting growth. Dietary lipid level af fects the optimal dietary E/P ratio. In conclusion, recommended dietary protein and energy for rare minnow are 20%–35% and 10–12.5 k J/g, respectively.
文摘Adjustments to rearing practices should be justified with increases in production, stocking success, or angler satisfaction. Largemouth bass (<span style="white-space:nowrap;"><i></span>Micropterus salmoides<span style="white-space:nowrap;"></i></span>) production was assessed between hatchery ponds where fish were restricted to an invertebrate diet or received supplemental fathead minnow (<span style="white-space:nowrap;"><i></span>Pimephales promelas<span style="white-space:nowrap;"></i></span>) forage during 2015. At harvest, age-0 bass yield was 4.5 times greater and average fish length was 38 mm longer, in the pond that received fathead minnow. In 2016, a second study evaluated the timing of minnow supplementation that included earlier stockings of small fathead minnow (<30 mm) compared to delayed supplementation with larger (>30 mm) minnows. With earlier supplementation, bass yield was 2.3 times greater and fish averaged 14 mm longer at harvest. Bass survival was approximately 38% higher during 2015 when supplementation occurred and 25% higher during 2016 when minnow supplementation began earlier. Our findings show invertebrate forage was probably limiting bass production in hatchery ponds and supplementing with appropriately-sized fathead minnows increased age-0, largemouth bass production.
基金Supported by the National Natural Science Foundation of China (Grant Nos. 40503014 and 40590392)Chinese Academy of Sciences (Grant No. KJCX2-SW- H06, KZCX2-YW-402)Beijing Nova Programme (Grant No. 2004A51)
文摘Based on the simulative exposure experiments of nitrobenzene to two small experimental fishes, Medaka (Oryzias latipes) and Chinese Rare Minnow (Gobiocypris rarus), it was found that nitrobenzene could decrease in a linear way in the static aquatic system. Both fishes could accumulate dose-related levels of nitrobenzene quickly and eliminate the compound rapidly when they were transferred to clean water. The Chinese rare minnow showed more sensitivity to the acute toxicity exposure of nitrobenzene than Medaka. Typical molecular biomarkers in oxidative defense system including superoxide dismu- tase (SOD), catalase (CAT) showed the chemical induced alterations. The changes of acetylcholi- nesterase (AChE) activities indicated that nitrobenzene might affect the normal neural function. Nitro- benzene exposure could also lead to obvious damaging effects on the target organs, such as gill and liver by hitopathological studies.
基金supported by the National“863”Program,the State High Tech Development Plan(Grant No.2001AA640610)the Chinese Academy of Sciences(Grant No.KZCX2-414).
文摘The acute (12 h) and chronic (7 or 14 d) effects of tributyltin chloride (TBT) on a new type of test fish, the Chinese rare minnow (Gobiocypris rarus), have been studied. High accumula-tion of the pollutant in the fish muscle and obvious changes in hepatosomatic index and gonad somatic index were observed after exposure. The ultrastructural findings show a series of patho-logical changes existing in the exposed gill cells and hepatocytes, which demonstrates the toxicity effects of tributyltin compounds on aquatic organisms. The results clearly show that the Chinese rare minnow is a new sensitive test fish material, whose exposure system is suitable for the exten-sive organometallic toxicity studies.
文摘目的和方法稀有鮈鲫(Gobiocypris rarus)作为一种本土特有的小型鱼类,是我国正在标准化的化学品毒性测试生物之一。为评价稀有鮈鲫鱼类急性毒性实验中效应的稳定性和可重复性,研究优化筛选了重铬酸钾和3,4-二氯苯胺两种化学品,分别在单一实验室内及不同实验室间进行急性毒性验证实验。结果对于来源相同、体长固定的稀有鮈鲫,无论在实验室内还是实验室间,两组化学品的96 h LC50值均在x珋±2s的质量控制范围之内,所有结果体现了很好的稳定性和可重复性。结论根据研究获得的数据可建立一个有效的稳定性和可重复性衡量数据库,用来评估之后其他单个实验的可靠性;稀有鮈鲫作为一种具有潜力的生态毒性测试的生物种类,具有成为标准实验动物的潜能。
文摘我国本土水生生物稀有鮈鲫(Gobiocypris rarus Ye et Fu,1983年)是一种优良的试验用鱼,具备成为模式生物必要条件。本文总结了稀有鮈鲫生物生态学研究情况,分析了稀有鮈鲫在多个应用领域与模式生物斑马鱼、青鳉存在的不足和差距,同时借鉴发达国家推动模式生物的实践经验,提出了针对性建议。
