Three common species of Miniopterus fuliginosus,M.magnater and M.pusillus are known to inhabit China.However,M.fuliginosus and M.magnater are so similar in external morphology as to pose great challenges for accurate ...Three common species of Miniopterus fuliginosus,M.magnater and M.pusillus are known to inhabit China.However,M.fuliginosus and M.magnater are so similar in external morphology as to pose great challenges for accurate classification.Furthermore,taxonomic statuses,distribution ranges and taxonomic keys of these three species have remained controversial.For addressing these outstanding issues,the authors integrated molecular phylogenetic analyses,ensemble species distribution models(ESDMs),multiple morphological comparisons and decision tree algorithms for reassessing their taxonomy and distribution in China.Mitochondrial cytochrome c oxidase subunit I(COI)gene phylogeny revealed three distinct monophyletic groups corresponding to M.fuliginosus,M.magnater and M.pusillus.And the observed distribution patterns indicated M.fuliginosus had a broad distribution across China while M.magnater and M.pusillus exhibited a more restricted distribution,overlapping with M.fuliginosus in South China.And cranial morphometry indicated M.magnater was slightly larger than M.fuliginosus and significantly larger than M.pusillus.Also three-dimensional(3D)skull geomorphometry uncovered distinct features for each species in rostrum,braincase,tympanic bullae and mandibular shape.Decision tree algorithms helped to identify forearm length,braincase breadth and width across the third upper molars as three major taxonomic keys for assisting species identification.This study corroborated the importance of integrative approaches for identifying Miniopterus species and validated a methodological approach applicable to other cryptic species complexes.展开更多
Coronaviruses, such as severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus, pose significant public health threats. Bats have been suggested to act as natural reservoirs for ...Coronaviruses, such as severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus, pose significant public health threats. Bats have been suggested to act as natural reservoirs for both these viruses, and periodic monitoring of coronaviruses in bats may thus provide important clues about emergent infectious viruses. The Eastern bent-wing bat Miniopterus fuliginosus is distributed extensively throughout China. We therefore analyzed the genetic diversity of coronaviruses in samples of M. fuliginosus collected from nine Chinese provinces during 2011–2013. The only coronavirus genus found was Alphacoronavirus. We established six complete and five partial genomic sequences of alphacoronaviruses, which revealed that they could be divided into two distinct lineages, with close relationships to coronaviruses in Miniopterus magnater and Miniopterus pusillus. Recombination was confirmed by detecting putative breakpoints of Lineage 1 coronaviruses in M. fuliginosus and M. pusillus(Wu et al., 2015), which supported the results of topological and phylogenetic analyses. The established alphacoronavirus genome sequences showed high similarity to other alphacoronaviruses found in other Miniopterus species, suggesting that their transmission in different Miniopterus species may provide opportunities for recombination with different alphacoronaviruses. The genetic information for these novel alphacoronaviruses will improve our understanding of the evolution and genetic diversity of coronaviruses, with potentially important implications for the transmission of human diseases.展开更多
基金the National Natural Sciences Foundation of China(32192421)the Special Grant Foundations for National Science and &Technology Basic Research Program of China(2021FY100303)the DFGP Project of Fauna of Guangdong Province(202115)。
文摘Three common species of Miniopterus fuliginosus,M.magnater and M.pusillus are known to inhabit China.However,M.fuliginosus and M.magnater are so similar in external morphology as to pose great challenges for accurate classification.Furthermore,taxonomic statuses,distribution ranges and taxonomic keys of these three species have remained controversial.For addressing these outstanding issues,the authors integrated molecular phylogenetic analyses,ensemble species distribution models(ESDMs),multiple morphological comparisons and decision tree algorithms for reassessing their taxonomy and distribution in China.Mitochondrial cytochrome c oxidase subunit I(COI)gene phylogeny revealed three distinct monophyletic groups corresponding to M.fuliginosus,M.magnater and M.pusillus.And the observed distribution patterns indicated M.fuliginosus had a broad distribution across China while M.magnater and M.pusillus exhibited a more restricted distribution,overlapping with M.fuliginosus in South China.And cranial morphometry indicated M.magnater was slightly larger than M.fuliginosus and significantly larger than M.pusillus.Also three-dimensional(3D)skull geomorphometry uncovered distinct features for each species in rostrum,braincase,tympanic bullae and mandibular shape.Decision tree algorithms helped to identify forearm length,braincase breadth and width across the third upper molars as three major taxonomic keys for assisting species identification.This study corroborated the importance of integrative approaches for identifying Miniopterus species and validated a methodological approach applicable to other cryptic species complexes.
基金supported by the Program for Changjiang Scholars and Innovative Research Team in University of China (IRT13007)the National S&T Major Project “China Mega-Project for Infectious Disease” (2011ZX10004-001, 2014ZX10004001) from China+1 种基金the National Natural Science Foundation of China (81501773)the PUMC Youth Fund and Fundamental Research Funds for the Central Universities (3332015095, 3332015006)
文摘Coronaviruses, such as severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus, pose significant public health threats. Bats have been suggested to act as natural reservoirs for both these viruses, and periodic monitoring of coronaviruses in bats may thus provide important clues about emergent infectious viruses. The Eastern bent-wing bat Miniopterus fuliginosus is distributed extensively throughout China. We therefore analyzed the genetic diversity of coronaviruses in samples of M. fuliginosus collected from nine Chinese provinces during 2011–2013. The only coronavirus genus found was Alphacoronavirus. We established six complete and five partial genomic sequences of alphacoronaviruses, which revealed that they could be divided into two distinct lineages, with close relationships to coronaviruses in Miniopterus magnater and Miniopterus pusillus. Recombination was confirmed by detecting putative breakpoints of Lineage 1 coronaviruses in M. fuliginosus and M. pusillus(Wu et al., 2015), which supported the results of topological and phylogenetic analyses. The established alphacoronavirus genome sequences showed high similarity to other alphacoronaviruses found in other Miniopterus species, suggesting that their transmission in different Miniopterus species may provide opportunities for recombination with different alphacoronaviruses. The genetic information for these novel alphacoronaviruses will improve our understanding of the evolution and genetic diversity of coronaviruses, with potentially important implications for the transmission of human diseases.
