Objective To investigate the expression of the preferentially expressed antigen of melanoma （PRAME） gene in acute leukemia and its clinical significance. Methods The level of expressed PRAME mRNA in bone marrow mono...Objective To investigate the expression of the preferentially expressed antigen of melanoma （PRAME） gene in acute leukemia and its clinical significance. Methods The level of expressed PRAME mRNA in bone marrow mononuclear cells from 34 patients with acute leukemia （AL） and in 12 bone marrow samples from healthy volunteers was measured via RT-PCR. Correlation analyses between PRAME gene expression and the clinical characteristics （gender, age, white blood count, immunophenotype of leukemia, percentage of blast cells, and karyotype） of the patients were performed. Results The PRAME gene was expressed in 38.2% of all 34 patients, in 40.7% of the patients with acute myelogenous leukemia （AML, n=27）, and in 28.6% of the patients with acute lymphoblastic leukemia （ALL, n=7）, but was not expressed in the healthy volunteers. The difference in the expression levels between AML and ALL patients was statistically significant. The rate of gene expression was 80% in M~, 33.3% in M2, and 28.6% in M~. Gene expression was also found to be correlated with CDl5 and CD33 expression and abnormal karyotype, but not with age, gender; white blood count or percentage of blast cells. Conclusions The PRAME gene is highly expressed in acute leukemia and could be a useful marker to monitor minimal residual disease. This gene is also a candidate target for the immunotherapy of acute leukemia.展开更多
In this paper,the application of extraction principle for logic function minimiza-tion to multi-output case is studied.A defect in original algorithm in dealing with multi-outputextrema is made up,and on this base,thr...In this paper,the application of extraction principle for logic function minimiza-tion to multi-output case is studied.A defect in original algorithm in dealing with multi-outputextrema is made up,and on this base,three kinds of less-than terms in different conditions aredefined.In addition,three kinds of generalized definition of less-than terms are given,so as to findout more efficiently the covers with minimal number of terms and irredundant function outputs.This work makes the multi-output extraction principle closer to perfection.An algorithm basedon the work is presented.展开更多
目的:研究黑色素瘤特异性抗原(preferentially expressed antigen of melanoma,PRAME)基因在急性白血病中的表达及其临床意义。方法:应用逆转录—聚合酶链反应(RT-PCR)技术,检测自2009年5月到2010年5月收治的34例急性白血病患者和12例...目的:研究黑色素瘤特异性抗原(preferentially expressed antigen of melanoma,PRAME)基因在急性白血病中的表达及其临床意义。方法:应用逆转录—聚合酶链反应(RT-PCR)技术,检测自2009年5月到2010年5月收治的34例急性白血病患者和12例健康者骨髓单核细胞中PRAME基因的mRNA表达水平,并与患者临床资料行相关性分析结果:34例急性白血病患者中,PRAME基因表达率为38.2%(13/34),AML患者PRAME的阳性表达率为40.7%(11/27),ALL患者的阳性表达率为28.6%(2/7)。在AML各亚型中,M3型阳性率最高为80%,其次为M2(33.3%)和M5(28.6%)分别行PRAME基因阳性与阴性组细胞表面抗原的监测,两组CID15(P<0.05)及CD33(P<0.05)的表达率均有显著性差异。染色体核型异常患者PRAME阳性率为61.5%(8/13),明显高于染色体核型正常者28.6%(4/14)(P<0.05);患者的年龄、性别、白细胞数、骨髓中原始和幼稚细胞比例与PRAME基因mRNA的表达差异无统计学意义。结论:PRAME基因在急性白血病中表达率较高,可作为监测微小残留病(minimal residualdisease,MRD)的标志基因之一。PRAME有可能作为白血病特异性免疫治疗的靶抗原。展开更多
目的:克隆及表达特异性抗原(PRAME)基因的cDNA片段。方法:从人急性髓细胞白血病细胞系HL60提取总RNA,用逆转录聚合酶链反应(RT PCR)从中扩增出PRAME基因cDNA片段。将PRAME基因cDNA片段插入载体pGEM T easy中,经全自动序列分析仪测序正确...目的:克隆及表达特异性抗原(PRAME)基因的cDNA片段。方法:从人急性髓细胞白血病细胞系HL60提取总RNA,用逆转录聚合酶链反应(RT PCR)从中扩增出PRAME基因cDNA片段。将PRAME基因cDNA片段插入载体pGEM T easy中,经全自动序列分析仪测序正确后,再克隆至表达载体pGEX4T2中,构建高效表达克隆,并转化大肠杆菌进行表达。结果:1mmol/L异丙基βD硫代半乳糖苷(IPTG)诱导5h,PRAME蛋白表达即达高峰。结论:PRAME基因在极少数正常组织中低表达,而在白血病患者高表达,并编码被自体细胞毒T淋巴细胞识别的抗原,所以该基因有望成为肿瘤免疫治疗的新成员。展开更多
文摘Objective To investigate the expression of the preferentially expressed antigen of melanoma （PRAME） gene in acute leukemia and its clinical significance. Methods The level of expressed PRAME mRNA in bone marrow mononuclear cells from 34 patients with acute leukemia （AL） and in 12 bone marrow samples from healthy volunteers was measured via RT-PCR. Correlation analyses between PRAME gene expression and the clinical characteristics （gender, age, white blood count, immunophenotype of leukemia, percentage of blast cells, and karyotype） of the patients were performed. Results The PRAME gene was expressed in 38.2% of all 34 patients, in 40.7% of the patients with acute myelogenous leukemia （AML, n=27）, and in 28.6% of the patients with acute lymphoblastic leukemia （ALL, n=7）, but was not expressed in the healthy volunteers. The difference in the expression levels between AML and ALL patients was statistically significant. The rate of gene expression was 80% in M~, 33.3% in M2, and 28.6% in M~. Gene expression was also found to be correlated with CDl5 and CD33 expression and abnormal karyotype, but not with age, gender; white blood count or percentage of blast cells. Conclusions The PRAME gene is highly expressed in acute leukemia and could be a useful marker to monitor minimal residual disease. This gene is also a candidate target for the immunotherapy of acute leukemia.
文摘In this paper,the application of extraction principle for logic function minimiza-tion to multi-output case is studied.A defect in original algorithm in dealing with multi-outputextrema is made up,and on this base,three kinds of less-than terms in different conditions aredefined.In addition,three kinds of generalized definition of less-than terms are given,so as to findout more efficiently the covers with minimal number of terms and irredundant function outputs.This work makes the multi-output extraction principle closer to perfection.An algorithm basedon the work is presented.
文摘目的:克隆及表达特异性抗原(PRAME)基因的cDNA片段。方法:从人急性髓细胞白血病细胞系HL60提取总RNA,用逆转录聚合酶链反应(RT PCR)从中扩增出PRAME基因cDNA片段。将PRAME基因cDNA片段插入载体pGEM T easy中,经全自动序列分析仪测序正确后,再克隆至表达载体pGEX4T2中,构建高效表达克隆,并转化大肠杆菌进行表达。结果:1mmol/L异丙基βD硫代半乳糖苷(IPTG)诱导5h,PRAME蛋白表达即达高峰。结论:PRAME基因在极少数正常组织中低表达,而在白血病患者高表达,并编码被自体细胞毒T淋巴细胞识别的抗原,所以该基因有望成为肿瘤免疫治疗的新成员。
