In recent years,chiral inorganic nanomaterials have become promising candidates for applications in sensing,catalysis,biomedicine,and photonics.Plasmonic nanomaterials with an intrinsic chiral structure exhibit intrig...In recent years,chiral inorganic nanomaterials have become promising candidates for applications in sensing,catalysis,biomedicine,and photonics.Plasmonic nanomaterials with an intrinsic chiral structure exhibit intriguing geometry‑dependent optical chirality,which benefits the combination of plasmonic characteristics with chirality.Recent advances in the biomolecule‑directed geometric control of intrinsically chiral plasmonic nanomaterials have further provided great opportunities for their widespread applications in many emerging technological areas.In this review,we present the recent progress in biosensing using chiral inorganic nanomaterials,with a particular focus on electrochemical and enzyme‑mimicking catalytic approaches.This paper commences with a review of the basic tenets underlying chiral nanocatalysts,incorporating the chiral ligand‑induced mechanism and the architectures of intrinsically chiral nanostructures.Additionally,it methodically expounds upon the applications of chiral nanocatalysts in the realms of electrochemical biosensing and enzyme‑mimicking catalytic biosensing respectively.Conclusively,it proffers a prospective view of the hurdles and prospects that accompany the deployment of chiral nanoprobes for nascent biosensing applications.By rational design of the chiral nanoprobes,it is envisioned that biosensing with increasing sensitivity and resolution toward the single‑molecule level can be achieved,which will substantially promote sensing applications in many emerging interdisciplinary areas.展开更多
Bionic microfluidics is garnering increasing attention due to the superior fluidic performance enabled by biomimetic microstructures.Inspired by the unique structures of young pumpkin stems,we fabricate helicoidally p...Bionic microfluidics is garnering increasing attention due to the superior fluidic performance enabled by biomimetic microstructures.Inspired by the unique structures of young pumpkin stems,we fabricate helicoidally patterned microchannels with precisely controlled morphologies using the projection micro-stereolithography(PμSL)-based 3D printing technique.Our helicoidally patterned microchannels achieve approximately twice the liquid lifting height compared to similarly sized smooth microchannels.This improvement is attributed to the enhanced capillary force.The additional meniscus formed between the helicoidally patterned microstructures significantly contributes to the increased capillary effects.Furthermore,the underlying mechanisms of fluidic performance in helicoidally patterned microchannels are theorized using a newly developed equation,which is also employed to optimize the geometric parameters and fluidic performance of the biomimetic helicoidal microchannels.Additionally,our biomimetic helicoidally patterned microchannels facilitate a significant step-lifting phenomenon,mimicking tall trees'transpiration.The fluidic performance of our biomimetic helicoidally patterned microchannels show promise for applications in enhanced liquid lifting,step-lifting,clean-water production,and others.展开更多
Natural enzymes are able to precisely bind substrates and catalyze activities because of their distinct framework structures.To mimic this ability,chemists are designing framework structures that resemble real enzymes...Natural enzymes are able to precisely bind substrates and catalyze activities because of their distinct framework structures.To mimic this ability,chemists are designing framework structures that resemble real enzymes.The use of metal-organic frameworks(MOFs)to mimic natural enzymes has advanced recently;this paper reviews these developments.This research specifically focuses on how the catalytically active center of natural enzymes can be exactly replicated by carefully controlling the composition and structure of MOFs.By identifying and attaching to substrates,MOFs can accelerate changes in a manner akin to that of real enzymes.The role of MOFs in simulating catalytic processes,enzyme activity,and potential uses in brain chemistry are also investigated in this work.It also discusses the most recent MOF applications in detecting and treating chemical abnormalities of the brain.The report finishes with a discussion of future research areas and potential applications,providing useful insights for researchers in the subject.展开更多
Cow milk protein allergy(CMPA)is a prevalent food allergy in infancy.It often presents with symptoms that overlap with other conditions,such as gastroeso-phageal reflux disease,lactose intolerance,food protein-induced...Cow milk protein allergy(CMPA)is a prevalent food allergy in infancy.It often presents with symptoms that overlap with other conditions,such as gastroeso-phageal reflux disease,lactose intolerance,food protein-induced enterocolitis syndrome,and eosinophilic esophagitis.This diagnostic overlap makes distin-guishing CMPA from its mimics difficult,resulting in potential misdiagnoses and unnecessary dietary restrictions.This review aims to comprehensively analyze CMPA and its mimicking conditions,highlighting their clinical presentations,diagnostic approaches,and management strategies to enhance diagnostic accu-racy and optimize patient care.A systematic literature search was conducted using PubMed,Scopus,Web of Science,and Google Scholar,focusing on studies published within the last 20 years.Articles addressing CMPA and its mimicking conditions were selected,with data synthesized into comparative analyses of diagnostic methods and management strategies.Accurate differentiation between CMPA and its mimics requires a thorough clinical evaluation supported by diagnostic tests such as skin prick tests,serum-specific IgE,and oral food challenges.Misdiagnosis can lead to nutritional deficiencies,psychological stress,and increased healthcare costs.Emerging diagnostic technologies,including component-resolved diagnostics and cytokine profiling,offer promising avenues for improving accuracy.A multidisciplinary approach involving pediatricians,allergists,and dietitians is essential for precise diagnosis and effective management.Ongoing research and education are crucial to enhancing clinical outcomes and reducing the burden on families.展开更多
BACKGROUND Gallbladder rupture is common in laparoscopic cholecystectomy because the gallbladder is usually in acute or chronic inflammation status.The gallstones may sometime be spilled into the peritoneal cavity,res...BACKGROUND Gallbladder rupture is common in laparoscopic cholecystectomy because the gallbladder is usually in acute or chronic inflammation status.The gallstones may sometime be spilled into the peritoneal cavity,resulting in intra-abdominal ab-scess if the gallstones were not retrieved.The diagnosis of intra-abdominal ab-scess caused by unretrieved gallstone can usually be correctly identified in the routine imaging studies,such as abdominal ultrasonography or computed tomo-graphy(CT).Here we present a case of abscess formation from unretrieved gall-stone following laparoscopic cholecystectomy,which mimics the imaging findings of metastatic gallbladder ade-nocarcinoma.CASE SUMMARY This case described a 78-year-old man who received laparoscopic cholecystectomy and gallbladder adenocarcinoma was diagnosed after surgery.After adjuvant chemotherapy,the following up abdominal CT showed several small nodules at right upper abdomen and peritoneal carcinomatosis is considered.Repeated laparoscopic surgery for the excision of seeding tumor was conducted and the pathological diagnosis of the nodules and mass was inflammatory tissues and gallbladder stone.CONCLUSION Spilled gallstones are a common complication during laparoscopic cholecystectomy and some gallstones fail to be retrieved due to the size or the restricted view of laparoscopic surgery.For spilled gall bladder stones,surgeons may consider regular computerized tomography follow-up,and if necessary,laparoscopic examination can be used as a means of confirming the diagnostic and treatment.展开更多
Mercury(Hg^(2+))has been recognized as a global pollutant with a toxic,mobile,and persistent nature.It adversely affects the ecosystem and human health.Already developed biosensors for Hg^(2+)detection majorly suffer ...Mercury(Hg^(2+))has been recognized as a global pollutant with a toxic,mobile,and persistent nature.It adversely affects the ecosystem and human health.Already developed biosensors for Hg^(2+)detection majorly suffer from poor sensitivity and specificity.Herein,a colorimetric/fluorimetric dual-mode sensing approach is designed for the quantitative detection of Hg^(2+).This novel sensing approach utilizes nanofluorophores,i.e.,fluorescent copper nanoclusters-doped zirconia metal-organic framework(CuNCs@Zr-MOF)nanoconjugate(blue color)and N-methyl mesoporphyrin IX(NMM)(red color)in combination with peroxidase-mimicking G-quadruplex DNAzyme(PMDNAzyme).In the presence of Hg^(2+),dabcyl conjugated complementary DNA with T-T mismatches form the stable duplex with the CuNCs@Zr-MOF@G-quadruplex structure through T-Hg^(2+)-T base pairing.It causes the quenching of fluorescence of CuNCs@Zr-MOF(463 nm)due to the Förster resonance energy transfer(FRET)system.Moreover,the G-quadruplex(G4)structure of the aptamer enhances the fluorescence emission of NMM(610 nm).Besides this,the peroxidase-like activity of G4/hemin DNAzyme offers the colorimetric detection of Hg^(2+).The formation of duplex with PMDNAzyme increases the catalytic activity.This novel biosensing probe quantitatively detected Hg^(2+)using both fluorimetry and colorimetry approaches with a low detection limit of 0.59 and 36.3 nM,respectively.It was also observed that the presence of interfering metal ions in case of real aqueous samples does not affect the performance of this novel biosensing probe.These findings confirm the considerable potential of the proposed biosensing probe to screen the concentration of Hg^(2+)in aquatic products.展开更多
Nanofibers can mimic natural tissue structure by creating a more suitable environment for cells to grow,prompting a wide application of nanofiber materials.In this review,we include relevant studies and characterize t...Nanofibers can mimic natural tissue structure by creating a more suitable environment for cells to grow,prompting a wide application of nanofiber materials.In this review,we include relevant studies and characterize the effect of nanofibers on mesenchymal stem cells,as well as factors that affect cell adhesion and osteogenic differentiation.We hypothesize that the process of bone regeneration in vitro is similar to bone formation and healing in vivo,and the closer nanofibers or nanofibrous scaffolds are to natural bone tissue,the better the bone regeneration process will be.In general,cells cultured on nanofibers have a similar gene expression pattern and osteogenic behavior as cells induced by osteogenic supplements in vitro.Genes involved in cell adhesion(focal adhesion kinase(FAK)),cytoskeletal organization,and osteogenic pathways(transforming growth factor-β(TGF-β)/bone morphogenic protein(BMP),mitogen-activated protein kinase(MAPK),and Wnt)are upregulated successively.Cell adhesion and osteogenesis may be influenced by several factors.Nanofibers possess certain physical properties including favorable hydrophilicity,porosity,and swelling properties that promote cell adhesion and growth.Moreover,nanofiber stiffness plays a vital role in cell fate,as cell recruitment for osteogenesis tends to be better on stiffer scaffolds,with associated signaling pathways of integrin and Yes-associated protein(YAP)/transcriptional co-activator with PDZ-binding motif(TAZ).Also,hierarchically aligned nanofibers,as well as their combination with functional additives(growth factors,HA particles,etc.),contribute to osteogenesis and bone regeneration.In summary,previous studies have indicated that upon sensing the stiffness of the nanofibrous environment as well as its other characteristics,stem cells change their shape and tension accordingly,regulating downstream pathways followed by adhesion to nanofibers to contribute to osteogenesis.However,additional experiments are needed to identify major signaling pathways in the bone regeneration process,and also to fully investigate its supportive role in fabricating or designing the optimum tissue-mimicking nanofibrous scaffolds.展开更多
We prepared the Fe3O4/g‐C3N4nanoparticles(NPs)through a simple electrostatic self‐assembly method with a3:97weight ratio to investigate their Fenton,photo‐Fenton and oxidative functionalities besides photocatalytic...We prepared the Fe3O4/g‐C3N4nanoparticles(NPs)through a simple electrostatic self‐assembly method with a3:97weight ratio to investigate their Fenton,photo‐Fenton and oxidative functionalities besides photocatalytic functionality.We observed an improvement of the Fenton and photo‐Fenton activities of the Fe3O4/g‐C3N4nanocomposites.This improvement was attributed to efficient charge transfer between Fe3O4and g‐C3N4at the heterojunctions,inhibition of electron‐hole recombination,a high surface area,and stabilization of Fe3O4against leaching by the hydrophobic g‐C3N4.The obtained NPs showed a higher degradation potential for rhodamine B(RhB)dye than those of Fe3O4and g‐C3N4.As compared to photocatalysis,the efficiency of RhB degradation in the Fenton and photo‐Fenton reactions was increased by20%and90%,respectively.Additionally,the horseradish peroxidase(HRP)activity of the prepared nanomaterials was studied with3,3,5,5‐tetramethylbenzidinedihydrochloride(TMB)as a substrate.Dopamine oxidation was also examined.Results indicate that Fe3O4/g‐C3N4nanocomposites offers more efficient degradation of RhB dye in a photo‐Fenton system compared with regular photocatalytic degradation,which requires a long time.Our study also confirmed that Fe3O4/g‐C3N4nanocomposites can be used as a potential material for mimicking HRP owing to its high affinity for TMB.These findings suggest good potential for applications in biosensing and as a catalyst in oxidation reactions.展开更多
BACKGROUND: Pyogenic hepatic abscess may mimic primary or secondary carcinoma of the liver on contrast-enhanced computed tomography(CECT). The present study was to explore the usefulness of the analysis of multislice-...BACKGROUND: Pyogenic hepatic abscess may mimic primary or secondary carcinoma of the liver on contrast-enhanced computed tomography(CECT). The present study was to explore the usefulness of the analysis of multislice-based texture acquired from CECT in the differentiation between pyogenic hepatic abscesses and malignant mimickers.METHODS: This retrospective study included 25 abscesses in 20 patients and 33 tumors in 26 subjects who underwent CECT. To make comparison, we also enrolled 19 patients with hepatic single simple cyst. The images from CECT were analyzed using a Laplacian of Gaussian band-pass filter(5 filter levels with sigma weighting ranging from 1.0 to 2.5). We also quantified the uniformity, entropy, kurtosis and skewness of the multislice-based texture at different sigma weightings. Statistical significance for these parameters was tested with oneway ANOVA followed by Tukey honestly significant difference(HSD) test. Diagnostic performance was evaluated using the receiver operating characteristic(ROC) curve analysis.RESULTS: There were significant differences in entropy and uniformity at all sigma weightings(P〈0.001) among hepatic abscesses, malignant mimickers and simple cysts. The significant difference in kurtosis and skewness was shown at sigma 1.8 and 2.0 weightings(P=0.002-0.006). Tukey HSD testshowed that the abscesses had a significantly higher entropy and lower uniformity compared with malignant mimickers(P=0.000-0.004). Entropy(at a sigma 2.0 weighting) had the largest area under the ROC curve(0.888) in differentiating abscesses from malignant mimickers, with a sensitivity of 81.8% and a specificity of 88.0% when the cutoff value was set to 3.64.CONCLUSION: Multislice-based texture analysis may be useful for differentiating pyogenic hepatic abscesses from malignant mimickers.展开更多
BACKGROUND Disc herniation refers to the displacement of disc material beyond its anatomical space.Disc sequestration is defined as migration of the herniated disc fragment into the epidural space,completely separatin...BACKGROUND Disc herniation refers to the displacement of disc material beyond its anatomical space.Disc sequestration is defined as migration of the herniated disc fragment into the epidural space,completely separating it from the parent disc.The fragment can move in upward,inferior,and lateral directions,which often causes low back pain and discomfort,abnormal sensation,and movement of lower limbs.The free disc fragments detached from the parent disc often mimic spinal tumors.Tumor like lumbar disc herniation can cause clinical symptoms similar to spinal tumors,such as lumbar soreness,pain,numbness and weakness of lower limbs,radiation pain of lower limbs,etc.It is usually necessary to diagnose the disease according to the doctor’s clinical experience,and make preliminary diagnosis and differential diagnosis with the help of magnetic resonance imaging(MRI)and contrast-enhanced MRI.However,pathological examination is the gold standard that distinguishes tumoral from non-tumoral status.We report four cases of disc herniation mimicking a tumor,and all the pathological results were intervertebral disc tissue.CASE SUMMARY The first case was a 71-year-old man with low back pain accompanied by left lower extremity radiating pain for 1 year,with exacerbation over the last 2 wk.After admission,MRI revealed a circular T2-hypointense lesion in the spinal canal of the L4 vertebral segment,with enhancement on contrast-enhanced MRI suggesting neurilemmoma.The second case was a 74-year-old man with pain in both knees associated with movement limitation for 3 years,with exacerbation over the last 3 mo.MRI revealed an oval T2-hyperintense lesion in the spinal canal at the L4–5 level,with obvious peripheral enhancement on contrast-enhanced MRI.Thus,neurilemmoma was suspected.The third case was a 53-year-old man who presented with numbness and weakness of the lumbar spine and right lower extremity for 2 wk.MRI revealed a round T2-hyperintense lesion in the spinal canal at the L4–5 level,with obvious rim enhancement on contrast-enhanced MRI.Thus,a spinal tumor was suspected.The fourth case was a 75-year-old man with right lower extremity pain for 2 wk,with exacerbation over the last week.MRI revealed a round T1-isointense lesion in the spinal canal of the L3 vertebral segment and a T2-hyperintense signal from the lesion.There was no obvious enhancement on contrast-enhanced MRI,so a spinal tumor was suspected.All four patients underwent surgery and recovered to ASIA grade E on postoperative days 5,8,8,and 6,respectively.All patients had an uneventful postoperative course and fully recovered within 3 mo.CONCLUSION Disc herniation mimicking a tumor is a relatively rare clinical entity and can be easily misdiagnosed as a spinal tumor.Examinations and tests should be improved preoperatively.Patients should undergo comprehensive preoperative evaluations,and the lesions should be removed surgically and confirmed by pathological diagnosis.展开更多
Cytomegalovirus(CMV) infection is associated with adverse clinical outcomes in immunosuppressed persons.The incidence and association of CMV reactivation with adverse clinical outcomes in critically ill persons lackin...Cytomegalovirus(CMV) infection is associated with adverse clinical outcomes in immunosuppressed persons.The incidence and association of CMV reactivation with adverse clinical outcomes in critically ill persons lacking evidence of immunosuppression at ICU admission has received great attention in the practice of critical care medicine.Critically ill patients in ICU who had associated risk factors such as mechanical ventilation,severe sepsis, or blood transfusion are more prone to CMV activation,which in turn led to increased mortality and morbidity in tenns of increased ICU stay,longer duration of mechanical ventilation,and higher rates of nosocomial infections.However,severe CMV as initial presentation mimicking dengue infection is rare.We recently came across seven cases with positive CMV serology at ICU admission,which we discuss in the light of current literature.展开更多
Mimicking compositional and constructional features of the extracellular matrix(ECM)is an effective parameter in improving the biological response of biomaterials.In this regard,carbon nanotube(CNT)and gelatin were ad...Mimicking compositional and constructional features of the extracellular matrix(ECM)is an effective parameter in improving the biological response of biomaterials.In this regard,carbon nanotube(CNT)and gelatin were added to magnesium-calcium phosphate cement(CNG)to mimic fibrillar construction and organic composition of ECM,respectively,besides the CNG performance was compared with the plain and CNT-reinforced cement.Cementation behavior of the cements was investigated by evaluating their setting time,cement composition variation during setting,and viscosity fluctuation.Furthermore,compressive strength,degradation,and cell response of the cements were compared.Adding 5 wt.%gelatin reduced setting time about 60%,because of gel formation,not due to struvite precipitation.Moreover,the gelatin decreased compressive strength by about 20%.Although gelatin decreased compressive strength,the strength remained in the range attributed to trabecular bone.All the types of cement indicated shear thinning behavior that made their injectability feasible.Compared to other types of cement,CNG enhanced proliferation and differentiation of mesenchymal stem cells besides faster degradation,nontoxicity and suitable cell adhesion.Hence,mimicking features of CNG enhanced osteoconductivity and osteoinductivity of the cement compared to the plain one.展开更多
To the Editor:IgG4-related sclerosing cholangitis（IgG4-SC）has re cently been recognized as a biliary manifestation of IgG4-related disease（IgG4-RD）.Type 3 IgG4-SC pre sented biliary strictures in both the porta he...To the Editor:IgG4-related sclerosing cholangitis（IgG4-SC）has re cently been recognized as a biliary manifestation of IgG4-related disease（IgG4-RD）.Type 3 IgG4-SC pre sented biliary strictures in both the porta hepatis and the distal common bile duct（CBD）.;Its manifestation especially in the absence of autoimmune pancreatitis,is extremely rare and very similar to that of cholangiocarci noma（CC）.展开更多
Several crucial stromal cell populations regulate hematopoiesis and malignant diseases in bone marrow niches.Precise regulation of these cell types can remodel niches and develop new therapeutics.Multiple nanocarriers...Several crucial stromal cell populations regulate hematopoiesis and malignant diseases in bone marrow niches.Precise regulation of these cell types can remodel niches and develop new therapeutics.Multiple nanocarriers have been developed to transport drugs into the bone marrow selectively.However,the delivery efficiency of these nanotherapeutics into crucial niche cells is still unknown,and there is no method available for predicting delivery efficiency in these cell types.Here,we constructed a three-dimensional bone marrow niche composed of three crucial cell populations:endothelial cells(ECs),mesenchymal stromal cells(MSCs),and osteoblasts(OBs).Mimetic niches were used to detect the cellular uptake of three typical drug nanocarriers into ECs/MSCs/OBs in vitro.Less than 5%of nanocarriers were taken up by three stromal cell types,and most of themwere located in the extracellular matrix.Delivery efficiency in sinusoidal ECs,arteriole ECs,MSCs,and OBs in vivo was analyzed.The correlation analysis showed that the cellular uptake of three nanocarriers in crucial cell types in vitro is positively linear correlated with its delivery efficiency in vivo.The delivery efficiency into MSCs was remarkably higher than that into ECs and OBs,no matterwhat kind of nanocarrier.The overall efficiency into sinusoidal ECswas greatly lower than that into arteriole ECs.All nanocarriers were hard to be delivered into OBs(<1%).Our findings revealed that cell tropisms of nanocarriers with different compositions and ligand attachments in vivo could be predicted via detecting their cellular uptake in bone marrow niches in vitro.This study provided the methodology for niche-directed nanotherapeutics development.展开更多
To explore construction of novel mimicking biomembrane on biomaterials surfaces, a new polymerizable phosphatidylcholine containing a long monoalkyl chain ended with acryl group （AASOPC） was designed and synthesized...To explore construction of novel mimicking biomembrane on biomaterials surfaces, a new polymerizable phosphatidylcholine containing a long monoalkyl chain ended with acryl group （AASOPC） was designed and synthesized, which was easily derived from the terminal amino group of 9-（2-amino-ethylcarbamoyl）-nonyl-l-phosphatidyl-choline （ASOPC） reacting with acryloyl chloride. The obtained AASOPC was grafted on poly（ethylene terephthalate） （PET） via surface-initiated atom-transfer radical polymerization （SI-ATRP） to form mimicking biomembrane. These modified surface structures of PET were investigated using water contact angle （WAC）, X-ray photoelectron spectroscopy （XPS） and atomic force microscopy （AFM）. The results indicated that the new mimicking phosphatidylcholine biomembrane could be prepared on inert polymer surfaces by using the acryloyl phosphatidylcholine （AASOPC） via surface-initiated atom transfer radical polymerization （SI-ATRP）.展开更多
Despite the considerable mortality and morbidity in metastasis cardiac mass, antemortem diagnosis is unusual. Here we report a case of a left atrial mass echocardiographically mimicking myxoma, in a patient with lung ...Despite the considerable mortality and morbidity in metastasis cardiac mass, antemortem diagnosis is unusual. Here we report a case of a left atrial mass echocardiographically mimicking myxoma, in a patient with lung carcinoma. The mass was pathologically confirmed to be metastatic carcinoma, which had entered the left pulmonary vein, illustrating the potential for unusual routes of tumor spread into tissue diagnosis in such cases atrium via direct invasion of left superior the heart and the importance of obtaining a展开更多
Dear editor, We report a case of a 47-year-old female who presented with a toxic bupropion ingestion leading to cardiac arrest. She initially exhibited a loss of brainstem reflexes in conjunction with burst-suppressio...Dear editor, We report a case of a 47-year-old female who presented with a toxic bupropion ingestion leading to cardiac arrest. She initially exhibited a loss of brainstem reflexes in conjunction with burst-suppression pattern on EEG. Burst suppression is an EEG waveform pattern of alternating isoelectric suppressions and high voltage bursts, Our patient ultimately made a full neurologic recovery a few days later. While there are two other cases in the literature of bupropion overdose resulting in EEG burst-suppression and loss of brainstem reflexes, we believe this is the only reported adult case complicated by cardiac arrest.展开更多
Dear Sir, I am B. Spajic, the urologist from Clinical Department of Urology, Sestre Milosrdnice University Hospital, Zagreb, Croatia. Recently, we had a rare case of a cholesterol granuloma of the right epididymis at...Dear Sir, I am B. Spajic, the urologist from Clinical Department of Urology, Sestre Milosrdnice University Hospital, Zagreb, Croatia. Recently, we had a rare case of a cholesterol granuloma of the right epididymis at our department, showing clinical signs of acute scrotum. The case described here appears to be the second reporting cholesterol granuloma in the epididymis and the first one presenting with clinical signs of acute scrotum.展开更多
Prostate cancer is the second most common cancer in men worldwide and the commonest cause of mortality in men. It is the commonest diagnosed cancer in African men. The earliest and common sites of metastasis are the a...Prostate cancer is the second most common cancer in men worldwide and the commonest cause of mortality in men. It is the commonest diagnosed cancer in African men. The earliest and common sites of metastasis are the axial skeleton and regional lymph nodes. Initial presentation of the metastatic disease with cervical or supraclavicular lymphadenopathy is rarely reported. We report 3 cases of metastatic cancer of the prostate presenting initially to physicians with huge cervical and supraclavicular lymph nodes enlargement which on biopsy revealed metastatic adenocarcinoma. On further evaluation, there were non-bothersome storage symptoms, elevated prostate specific antigen and abnormal digital rectal examination. Transrectal ultrasound (TRUS)-guided biopsy of hypoechoic nodules revealed high-grade adenocarcinoma of the prostate on histopathological examination. The cervical and supraclavicular lymphadenopathy resolved after commencement of androgen deprivation therapy. We advocate for prostate cancer screening in African men above 50 years of age presenting with cervical or supraclavicular lymphadenopthy to primary care physicians even in the absence of lower urinary tract symptoms.展开更多
文摘In recent years,chiral inorganic nanomaterials have become promising candidates for applications in sensing,catalysis,biomedicine,and photonics.Plasmonic nanomaterials with an intrinsic chiral structure exhibit intriguing geometry‑dependent optical chirality,which benefits the combination of plasmonic characteristics with chirality.Recent advances in the biomolecule‑directed geometric control of intrinsically chiral plasmonic nanomaterials have further provided great opportunities for their widespread applications in many emerging technological areas.In this review,we present the recent progress in biosensing using chiral inorganic nanomaterials,with a particular focus on electrochemical and enzyme‑mimicking catalytic approaches.This paper commences with a review of the basic tenets underlying chiral nanocatalysts,incorporating the chiral ligand‑induced mechanism and the architectures of intrinsically chiral nanostructures.Additionally,it methodically expounds upon the applications of chiral nanocatalysts in the realms of electrochemical biosensing and enzyme‑mimicking catalytic biosensing respectively.Conclusively,it proffers a prospective view of the hurdles and prospects that accompany the deployment of chiral nanoprobes for nascent biosensing applications.By rational design of the chiral nanoprobes,it is envisioned that biosensing with increasing sensitivity and resolution toward the single‑molecule level can be achieved,which will substantially promote sensing applications in many emerging interdisciplinary areas.
基金supported by National Natural Science Foundation of China through Grant Nos.52495000,52332012 and 52176093partially supported by Beijing Huiyangdao Health Technology Co.,Ltd。
文摘Bionic microfluidics is garnering increasing attention due to the superior fluidic performance enabled by biomimetic microstructures.Inspired by the unique structures of young pumpkin stems,we fabricate helicoidally patterned microchannels with precisely controlled morphologies using the projection micro-stereolithography(PμSL)-based 3D printing technique.Our helicoidally patterned microchannels achieve approximately twice the liquid lifting height compared to similarly sized smooth microchannels.This improvement is attributed to the enhanced capillary force.The additional meniscus formed between the helicoidally patterned microstructures significantly contributes to the increased capillary effects.Furthermore,the underlying mechanisms of fluidic performance in helicoidally patterned microchannels are theorized using a newly developed equation,which is also employed to optimize the geometric parameters and fluidic performance of the biomimetic helicoidal microchannels.Additionally,our biomimetic helicoidally patterned microchannels facilitate a significant step-lifting phenomenon,mimicking tall trees'transpiration.The fluidic performance of our biomimetic helicoidally patterned microchannels show promise for applications in enhanced liquid lifting,step-lifting,clean-water production,and others.
基金financially supported by the National Natural Science Foundation,China(Nos.22074095&22374103(Y.Lin))Beijing Natural Science Foundation(No.2222005(Y.Lin))。
文摘Natural enzymes are able to precisely bind substrates and catalyze activities because of their distinct framework structures.To mimic this ability,chemists are designing framework structures that resemble real enzymes.The use of metal-organic frameworks(MOFs)to mimic natural enzymes has advanced recently;this paper reviews these developments.This research specifically focuses on how the catalytically active center of natural enzymes can be exactly replicated by carefully controlling the composition and structure of MOFs.By identifying and attaching to substrates,MOFs can accelerate changes in a manner akin to that of real enzymes.The role of MOFs in simulating catalytic processes,enzyme activity,and potential uses in brain chemistry are also investigated in this work.It also discusses the most recent MOF applications in detecting and treating chemical abnormalities of the brain.The report finishes with a discussion of future research areas and potential applications,providing useful insights for researchers in the subject.
文摘Cow milk protein allergy(CMPA)is a prevalent food allergy in infancy.It often presents with symptoms that overlap with other conditions,such as gastroeso-phageal reflux disease,lactose intolerance,food protein-induced enterocolitis syndrome,and eosinophilic esophagitis.This diagnostic overlap makes distin-guishing CMPA from its mimics difficult,resulting in potential misdiagnoses and unnecessary dietary restrictions.This review aims to comprehensively analyze CMPA and its mimicking conditions,highlighting their clinical presentations,diagnostic approaches,and management strategies to enhance diagnostic accu-racy and optimize patient care.A systematic literature search was conducted using PubMed,Scopus,Web of Science,and Google Scholar,focusing on studies published within the last 20 years.Articles addressing CMPA and its mimicking conditions were selected,with data synthesized into comparative analyses of diagnostic methods and management strategies.Accurate differentiation between CMPA and its mimics requires a thorough clinical evaluation supported by diagnostic tests such as skin prick tests,serum-specific IgE,and oral food challenges.Misdiagnosis can lead to nutritional deficiencies,psychological stress,and increased healthcare costs.Emerging diagnostic technologies,including component-resolved diagnostics and cytokine profiling,offer promising avenues for improving accuracy.A multidisciplinary approach involving pediatricians,allergists,and dietitians is essential for precise diagnosis and effective management.Ongoing research and education are crucial to enhancing clinical outcomes and reducing the burden on families.
文摘BACKGROUND Gallbladder rupture is common in laparoscopic cholecystectomy because the gallbladder is usually in acute or chronic inflammation status.The gallstones may sometime be spilled into the peritoneal cavity,resulting in intra-abdominal ab-scess if the gallstones were not retrieved.The diagnosis of intra-abdominal ab-scess caused by unretrieved gallstone can usually be correctly identified in the routine imaging studies,such as abdominal ultrasonography or computed tomo-graphy(CT).Here we present a case of abscess formation from unretrieved gall-stone following laparoscopic cholecystectomy,which mimics the imaging findings of metastatic gallbladder ade-nocarcinoma.CASE SUMMARY This case described a 78-year-old man who received laparoscopic cholecystectomy and gallbladder adenocarcinoma was diagnosed after surgery.After adjuvant chemotherapy,the following up abdominal CT showed several small nodules at right upper abdomen and peritoneal carcinomatosis is considered.Repeated laparoscopic surgery for the excision of seeding tumor was conducted and the pathological diagnosis of the nodules and mass was inflammatory tissues and gallbladder stone.CONCLUSION Spilled gallstones are a common complication during laparoscopic cholecystectomy and some gallstones fail to be retrieved due to the size or the restricted view of laparoscopic surgery.For spilled gall bladder stones,surgeons may consider regular computerized tomography follow-up,and if necessary,laparoscopic examination can be used as a means of confirming the diagnostic and treatment.
基金Funding:S.K.thanks the Department of Biotechnology(DBT),Government of India,for research grant(award BT/PR18868/BCE/8/1370/2016 dated 2018 January 31)M.N.is grateful to CSIR for the SRA fellowship(no.B-12857,dated 2021 October 21).
文摘Mercury(Hg^(2+))has been recognized as a global pollutant with a toxic,mobile,and persistent nature.It adversely affects the ecosystem and human health.Already developed biosensors for Hg^(2+)detection majorly suffer from poor sensitivity and specificity.Herein,a colorimetric/fluorimetric dual-mode sensing approach is designed for the quantitative detection of Hg^(2+).This novel sensing approach utilizes nanofluorophores,i.e.,fluorescent copper nanoclusters-doped zirconia metal-organic framework(CuNCs@Zr-MOF)nanoconjugate(blue color)and N-methyl mesoporphyrin IX(NMM)(red color)in combination with peroxidase-mimicking G-quadruplex DNAzyme(PMDNAzyme).In the presence of Hg^(2+),dabcyl conjugated complementary DNA with T-T mismatches form the stable duplex with the CuNCs@Zr-MOF@G-quadruplex structure through T-Hg^(2+)-T base pairing.It causes the quenching of fluorescence of CuNCs@Zr-MOF(463 nm)due to the Förster resonance energy transfer(FRET)system.Moreover,the G-quadruplex(G4)structure of the aptamer enhances the fluorescence emission of NMM(610 nm).Besides this,the peroxidase-like activity of G4/hemin DNAzyme offers the colorimetric detection of Hg^(2+).The formation of duplex with PMDNAzyme increases the catalytic activity.This novel biosensing probe quantitatively detected Hg^(2+)using both fluorimetry and colorimetry approaches with a low detection limit of 0.59 and 36.3 nM,respectively.It was also observed that the presence of interfering metal ions in case of real aqueous samples does not affect the performance of this novel biosensing probe.These findings confirm the considerable potential of the proposed biosensing probe to screen the concentration of Hg^(2+)in aquatic products.
基金the National Natural Science Foundation of China(No.31570989)the Young Talents Project of Zhejiang Provincial Health Department(No.2019RC151)the Zhejiang Provincial Welfare Technology Research Project(No.LGF20H140007),China。
文摘Nanofibers can mimic natural tissue structure by creating a more suitable environment for cells to grow,prompting a wide application of nanofiber materials.In this review,we include relevant studies and characterize the effect of nanofibers on mesenchymal stem cells,as well as factors that affect cell adhesion and osteogenic differentiation.We hypothesize that the process of bone regeneration in vitro is similar to bone formation and healing in vivo,and the closer nanofibers or nanofibrous scaffolds are to natural bone tissue,the better the bone regeneration process will be.In general,cells cultured on nanofibers have a similar gene expression pattern and osteogenic behavior as cells induced by osteogenic supplements in vitro.Genes involved in cell adhesion(focal adhesion kinase(FAK)),cytoskeletal organization,and osteogenic pathways(transforming growth factor-β(TGF-β)/bone morphogenic protein(BMP),mitogen-activated protein kinase(MAPK),and Wnt)are upregulated successively.Cell adhesion and osteogenesis may be influenced by several factors.Nanofibers possess certain physical properties including favorable hydrophilicity,porosity,and swelling properties that promote cell adhesion and growth.Moreover,nanofiber stiffness plays a vital role in cell fate,as cell recruitment for osteogenesis tends to be better on stiffer scaffolds,with associated signaling pathways of integrin and Yes-associated protein(YAP)/transcriptional co-activator with PDZ-binding motif(TAZ).Also,hierarchically aligned nanofibers,as well as their combination with functional additives(growth factors,HA particles,etc.),contribute to osteogenesis and bone regeneration.In summary,previous studies have indicated that upon sensing the stiffness of the nanofibrous environment as well as its other characteristics,stem cells change their shape and tension accordingly,regulating downstream pathways followed by adhesion to nanofibers to contribute to osteogenesis.However,additional experiments are needed to identify major signaling pathways in the bone regeneration process,and also to fully investigate its supportive role in fabricating or designing the optimum tissue-mimicking nanofibrous scaffolds.
基金supported by the National Natural Science Foundation of China(51572253,21771171)Scientific Research Grant of Hefei Science Center of CAS(2015SRG-HSC048)+1 种基金cooperation between NSFC and Netherlands Organization for Scientific Research(51561135011)CAS-TWAS Scholarship Program~~
文摘We prepared the Fe3O4/g‐C3N4nanoparticles(NPs)through a simple electrostatic self‐assembly method with a3:97weight ratio to investigate their Fenton,photo‐Fenton and oxidative functionalities besides photocatalytic functionality.We observed an improvement of the Fenton and photo‐Fenton activities of the Fe3O4/g‐C3N4nanocomposites.This improvement was attributed to efficient charge transfer between Fe3O4and g‐C3N4at the heterojunctions,inhibition of electron‐hole recombination,a high surface area,and stabilization of Fe3O4against leaching by the hydrophobic g‐C3N4.The obtained NPs showed a higher degradation potential for rhodamine B(RhB)dye than those of Fe3O4and g‐C3N4.As compared to photocatalysis,the efficiency of RhB degradation in the Fenton and photo‐Fenton reactions was increased by20%and90%,respectively.Additionally,the horseradish peroxidase(HRP)activity of the prepared nanomaterials was studied with3,3,5,5‐tetramethylbenzidinedihydrochloride(TMB)as a substrate.Dopamine oxidation was also examined.Results indicate that Fe3O4/g‐C3N4nanocomposites offers more efficient degradation of RhB dye in a photo‐Fenton system compared with regular photocatalytic degradation,which requires a long time.Our study also confirmed that Fe3O4/g‐C3N4nanocomposites can be used as a potential material for mimicking HRP owing to its high affinity for TMB.These findings suggest good potential for applications in biosensing and as a catalyst in oxidation reactions.
基金supported by grants from Medical Engineering Cross Research Foundation of Shanghai Jiaotong University(YG2013MS37 and YG2012MS16)the National Natural Science Foundation of China(81201172,81371660 and 81371622)
文摘BACKGROUND: Pyogenic hepatic abscess may mimic primary or secondary carcinoma of the liver on contrast-enhanced computed tomography(CECT). The present study was to explore the usefulness of the analysis of multislice-based texture acquired from CECT in the differentiation between pyogenic hepatic abscesses and malignant mimickers.METHODS: This retrospective study included 25 abscesses in 20 patients and 33 tumors in 26 subjects who underwent CECT. To make comparison, we also enrolled 19 patients with hepatic single simple cyst. The images from CECT were analyzed using a Laplacian of Gaussian band-pass filter(5 filter levels with sigma weighting ranging from 1.0 to 2.5). We also quantified the uniformity, entropy, kurtosis and skewness of the multislice-based texture at different sigma weightings. Statistical significance for these parameters was tested with oneway ANOVA followed by Tukey honestly significant difference(HSD) test. Diagnostic performance was evaluated using the receiver operating characteristic(ROC) curve analysis.RESULTS: There were significant differences in entropy and uniformity at all sigma weightings(P〈0.001) among hepatic abscesses, malignant mimickers and simple cysts. The significant difference in kurtosis and skewness was shown at sigma 1.8 and 2.0 weightings(P=0.002-0.006). Tukey HSD testshowed that the abscesses had a significantly higher entropy and lower uniformity compared with malignant mimickers(P=0.000-0.004). Entropy(at a sigma 2.0 weighting) had the largest area under the ROC curve(0.888) in differentiating abscesses from malignant mimickers, with a sensitivity of 81.8% and a specificity of 88.0% when the cutoff value was set to 3.64.CONCLUSION: Multislice-based texture analysis may be useful for differentiating pyogenic hepatic abscesses from malignant mimickers.
基金Supported by Chinese People’s Liberation Army Medical Technology Youth Training Program,No. 20QNPY071
文摘BACKGROUND Disc herniation refers to the displacement of disc material beyond its anatomical space.Disc sequestration is defined as migration of the herniated disc fragment into the epidural space,completely separating it from the parent disc.The fragment can move in upward,inferior,and lateral directions,which often causes low back pain and discomfort,abnormal sensation,and movement of lower limbs.The free disc fragments detached from the parent disc often mimic spinal tumors.Tumor like lumbar disc herniation can cause clinical symptoms similar to spinal tumors,such as lumbar soreness,pain,numbness and weakness of lower limbs,radiation pain of lower limbs,etc.It is usually necessary to diagnose the disease according to the doctor’s clinical experience,and make preliminary diagnosis and differential diagnosis with the help of magnetic resonance imaging(MRI)and contrast-enhanced MRI.However,pathological examination is the gold standard that distinguishes tumoral from non-tumoral status.We report four cases of disc herniation mimicking a tumor,and all the pathological results were intervertebral disc tissue.CASE SUMMARY The first case was a 71-year-old man with low back pain accompanied by left lower extremity radiating pain for 1 year,with exacerbation over the last 2 wk.After admission,MRI revealed a circular T2-hypointense lesion in the spinal canal of the L4 vertebral segment,with enhancement on contrast-enhanced MRI suggesting neurilemmoma.The second case was a 74-year-old man with pain in both knees associated with movement limitation for 3 years,with exacerbation over the last 3 mo.MRI revealed an oval T2-hyperintense lesion in the spinal canal at the L4–5 level,with obvious peripheral enhancement on contrast-enhanced MRI.Thus,neurilemmoma was suspected.The third case was a 53-year-old man who presented with numbness and weakness of the lumbar spine and right lower extremity for 2 wk.MRI revealed a round T2-hyperintense lesion in the spinal canal at the L4–5 level,with obvious rim enhancement on contrast-enhanced MRI.Thus,a spinal tumor was suspected.The fourth case was a 75-year-old man with right lower extremity pain for 2 wk,with exacerbation over the last week.MRI revealed a round T1-isointense lesion in the spinal canal of the L3 vertebral segment and a T2-hyperintense signal from the lesion.There was no obvious enhancement on contrast-enhanced MRI,so a spinal tumor was suspected.All four patients underwent surgery and recovered to ASIA grade E on postoperative days 5,8,8,and 6,respectively.All patients had an uneventful postoperative course and fully recovered within 3 mo.CONCLUSION Disc herniation mimicking a tumor is a relatively rare clinical entity and can be easily misdiagnosed as a spinal tumor.Examinations and tests should be improved preoperatively.Patients should undergo comprehensive preoperative evaluations,and the lesions should be removed surgically and confirmed by pathological diagnosis.
文摘Cytomegalovirus(CMV) infection is associated with adverse clinical outcomes in immunosuppressed persons.The incidence and association of CMV reactivation with adverse clinical outcomes in critically ill persons lacking evidence of immunosuppression at ICU admission has received great attention in the practice of critical care medicine.Critically ill patients in ICU who had associated risk factors such as mechanical ventilation,severe sepsis, or blood transfusion are more prone to CMV activation,which in turn led to increased mortality and morbidity in tenns of increased ICU stay,longer duration of mechanical ventilation,and higher rates of nosocomial infections.However,severe CMV as initial presentation mimicking dengue infection is rare.We recently came across seven cases with positive CMV serology at ICU admission,which we discuss in the light of current literature.
文摘Mimicking compositional and constructional features of the extracellular matrix(ECM)is an effective parameter in improving the biological response of biomaterials.In this regard,carbon nanotube(CNT)and gelatin were added to magnesium-calcium phosphate cement(CNG)to mimic fibrillar construction and organic composition of ECM,respectively,besides the CNG performance was compared with the plain and CNT-reinforced cement.Cementation behavior of the cements was investigated by evaluating their setting time,cement composition variation during setting,and viscosity fluctuation.Furthermore,compressive strength,degradation,and cell response of the cements were compared.Adding 5 wt.%gelatin reduced setting time about 60%,because of gel formation,not due to struvite precipitation.Moreover,the gelatin decreased compressive strength by about 20%.Although gelatin decreased compressive strength,the strength remained in the range attributed to trabecular bone.All the types of cement indicated shear thinning behavior that made their injectability feasible.Compared to other types of cement,CNG enhanced proliferation and differentiation of mesenchymal stem cells besides faster degradation,nontoxicity and suitable cell adhesion.Hence,mimicking features of CNG enhanced osteoconductivity and osteoinductivity of the cement compared to the plain one.
基金supported by a grant from the National Natural Science Foundation of China(81270544)
文摘To the Editor:IgG4-related sclerosing cholangitis（IgG4-SC）has re cently been recognized as a biliary manifestation of IgG4-related disease（IgG4-RD）.Type 3 IgG4-SC pre sented biliary strictures in both the porta hepatis and the distal common bile duct（CBD）.;Its manifestation especially in the absence of autoimmune pancreatitis,is extremely rare and very similar to that of cholangiocarci noma（CC）.
基金support from the National Natural Science Foundation of China(81703713,82174095,82274364)Natural Science Foundation of Zhejiang Province grants(LZ23H290001,LZ22H290001)internal support from Zhejiang Chinese Medical University(2022GJYY011).
文摘Several crucial stromal cell populations regulate hematopoiesis and malignant diseases in bone marrow niches.Precise regulation of these cell types can remodel niches and develop new therapeutics.Multiple nanocarriers have been developed to transport drugs into the bone marrow selectively.However,the delivery efficiency of these nanotherapeutics into crucial niche cells is still unknown,and there is no method available for predicting delivery efficiency in these cell types.Here,we constructed a three-dimensional bone marrow niche composed of three crucial cell populations:endothelial cells(ECs),mesenchymal stromal cells(MSCs),and osteoblasts(OBs).Mimetic niches were used to detect the cellular uptake of three typical drug nanocarriers into ECs/MSCs/OBs in vitro.Less than 5%of nanocarriers were taken up by three stromal cell types,and most of themwere located in the extracellular matrix.Delivery efficiency in sinusoidal ECs,arteriole ECs,MSCs,and OBs in vivo was analyzed.The correlation analysis showed that the cellular uptake of three nanocarriers in crucial cell types in vitro is positively linear correlated with its delivery efficiency in vivo.The delivery efficiency into MSCs was remarkably higher than that into ECs and OBs,no matterwhat kind of nanocarrier.The overall efficiency into sinusoidal ECswas greatly lower than that into arteriole ECs.All nanocarriers were hard to be delivered into OBs(<1%).Our findings revealed that cell tropisms of nanocarriers with different compositions and ligand attachments in vivo could be predicted via detecting their cellular uptake in bone marrow niches in vitro.This study provided the methodology for niche-directed nanotherapeutics development.
基金financially supported by the National Natural Science Foundation of China(No.20804025 and 50673063)Program for the New-Century Excellent Talents of Ministry of Education of China(NCET-08-0381)Sichuan Provincial Science Fund for Distinguished Young Scholars(09ZQ026-024)
文摘To explore construction of novel mimicking biomembrane on biomaterials surfaces, a new polymerizable phosphatidylcholine containing a long monoalkyl chain ended with acryl group （AASOPC） was designed and synthesized, which was easily derived from the terminal amino group of 9-（2-amino-ethylcarbamoyl）-nonyl-l-phosphatidyl-choline （ASOPC） reacting with acryloyl chloride. The obtained AASOPC was grafted on poly（ethylene terephthalate） （PET） via surface-initiated atom-transfer radical polymerization （SI-ATRP） to form mimicking biomembrane. These modified surface structures of PET were investigated using water contact angle （WAC）, X-ray photoelectron spectroscopy （XPS） and atomic force microscopy （AFM）. The results indicated that the new mimicking phosphatidylcholine biomembrane could be prepared on inert polymer surfaces by using the acryloyl phosphatidylcholine （AASOPC） via surface-initiated atom transfer radical polymerization （SI-ATRP）.
基金Supported by Natural Science Foundation of Chongqing in China (Grant No 30700822)
文摘Despite the considerable mortality and morbidity in metastasis cardiac mass, antemortem diagnosis is unusual. Here we report a case of a left atrial mass echocardiographically mimicking myxoma, in a patient with lung carcinoma. The mass was pathologically confirmed to be metastatic carcinoma, which had entered the left pulmonary vein, illustrating the potential for unusual routes of tumor spread into tissue diagnosis in such cases atrium via direct invasion of left superior the heart and the importance of obtaining a
文摘Dear editor, We report a case of a 47-year-old female who presented with a toxic bupropion ingestion leading to cardiac arrest. She initially exhibited a loss of brainstem reflexes in conjunction with burst-suppression pattern on EEG. Burst suppression is an EEG waveform pattern of alternating isoelectric suppressions and high voltage bursts, Our patient ultimately made a full neurologic recovery a few days later. While there are two other cases in the literature of bupropion overdose resulting in EEG burst-suppression and loss of brainstem reflexes, we believe this is the only reported adult case complicated by cardiac arrest.
文摘Dear Sir, I am B. Spajic, the urologist from Clinical Department of Urology, Sestre Milosrdnice University Hospital, Zagreb, Croatia. Recently, we had a rare case of a cholesterol granuloma of the right epididymis at our department, showing clinical signs of acute scrotum. The case described here appears to be the second reporting cholesterol granuloma in the epididymis and the first one presenting with clinical signs of acute scrotum.
文摘Prostate cancer is the second most common cancer in men worldwide and the commonest cause of mortality in men. It is the commonest diagnosed cancer in African men. The earliest and common sites of metastasis are the axial skeleton and regional lymph nodes. Initial presentation of the metastatic disease with cervical or supraclavicular lymphadenopathy is rarely reported. We report 3 cases of metastatic cancer of the prostate presenting initially to physicians with huge cervical and supraclavicular lymph nodes enlargement which on biopsy revealed metastatic adenocarcinoma. On further evaluation, there were non-bothersome storage symptoms, elevated prostate specific antigen and abnormal digital rectal examination. Transrectal ultrasound (TRUS)-guided biopsy of hypoechoic nodules revealed high-grade adenocarcinoma of the prostate on histopathological examination. The cervical and supraclavicular lymphadenopathy resolved after commencement of androgen deprivation therapy. We advocate for prostate cancer screening in African men above 50 years of age presenting with cervical or supraclavicular lymphadenopthy to primary care physicians even in the absence of lower urinary tract symptoms.
