Directional migration of leukocytes is indispensable to innate immunity for host defense. However, recruitment of leukocytes to a site of tissue injury also constitutes a leading cause for inflammatory responses. Mech...Directional migration of leukocytes is indispensable to innate immunity for host defense. However, recruitment of leukocytes to a site of tissue injury also constitutes a leading cause for inflammatory responses. Mechanistically, it involves a cascade of cellular events precisely regulated by temporal and spatial presentation of a repertoire of molecules in the migrating leukocytes and their surroundings (microenvironments). Here I will summarize the emerging evidence that has shed lights on the underlying molecular mechanism for directional migration of leukocytes, which has guided the therapeutical development for innovative anti-inflammatory medicines.展开更多
OBJECTIVE: To evaluate the effects of moxa-burning heat stimulating acupoints Zusanli(ST36) and Shenshu(BL23) on macrophage migration inhibitory factor(MIF) and its related molecules which can provide scientific exper...OBJECTIVE: To evaluate the effects of moxa-burning heat stimulating acupoints Zusanli(ST36) and Shenshu(BL23) on macrophage migration inhibitory factor(MIF) and its related molecules which can provide scientific experimental basis for the clinical application of moxibustion treatment of rheumatoid arthritis(RA). METHODS: Thirty rabbits were randomly assigned to control group, RA model(established by injecting Freund’s Complete Adjuvant) group(RA group) and RA model with moxibustion group [Moxa group, Zusanli(ST36) and Shenshu(BL23), 5 moxa pillars/day, 6 d × 3]. The expressions of MIF m RNA were evaluated with reverse transcription polymerase chain reaction;the apoptosis rates of macrophages were detected by erminal deoxynucleotidyl transferase-mediated d TUP nick end labeling;the expressions of related signal molecules were detected with immunohistochemical S-P method and the levels of IL-2 were detected with enzymelinked immunosorbent assay. RESULTS: The expressions of MIF m RNA, extracellular regulated protein kinases 2, p38 mitogen-activated protein kinase and nuclear factor-κ-gene binding p65 in synovial tissue of RA group were significantly increased when compared with control group, which were lower remarkably in moxa group than those in RA group. The apoptosis rates of macrophages in RA group were significantly down-regulated as compared with the control group, which were up-regulated in moxa group compared with the RA group. The levels of IL-2 in synovial fluid from the RA group were elevated significantly as compared with that from control group, but those of the moxa group were reduced when compared with those from RA group. CONCLUSIONS: Moxibustion may simultaneously regulate the expressions of MIF and its related signaling pathways molecules, the apoptosis rate of macrophages in synovial tissue, as well as the level of inflammatory factors in synovial fluid. The results suggest that the antiinflammatory effect of moxibustion on RA may be related to inhibit the expression of MIF in synovial tissue, the molecules of some related signaling pathways and promote the apoptosis of macrophage.展开更多
文摘Directional migration of leukocytes is indispensable to innate immunity for host defense. However, recruitment of leukocytes to a site of tissue injury also constitutes a leading cause for inflammatory responses. Mechanistically, it involves a cascade of cellular events precisely regulated by temporal and spatial presentation of a repertoire of molecules in the migrating leukocytes and their surroundings (microenvironments). Here I will summarize the emerging evidence that has shed lights on the underlying molecular mechanism for directional migration of leukocytes, which has guided the therapeutical development for innovative anti-inflammatory medicines.
基金Supported by National Natural Science Foundation of China:Study on the Immune Mechanisms of Macrophage M1/M2 Polarization in the Treatment of Rheumatoid Arthritis by Moxibustion “Strengthening Body Resistance and Eliminating Evil”(No. 81973959)National Natural Science Foundation of China:Research on “ImmuneInflammation” Molecular Signal Regulation of NLRP3 Inflammasomes in RA with Moxibustion Treatment (No. 81774435)+2 种基金National Key R&D Program of China:Research on the Functional Characterics of “Special Effect” and “Common Effect” of Acupoints (No. 2019YFC1709001)Foundation of Sichuan Provincial Administration of Traditional Chinese Medicine:Research on MIF-GC Rhythm Regulation Mechanism of Moxibustion in the Treatment of Rheumatoid Arthritis (No. 2018JC007)Foundation of Chengdu University of Traditional Chinese Medicine:Research on the Mechanism of “MIF-target Protein-GC-inflammation” of Antiinflammatory Effect of Moxibustion on Rheumatoid Arthritis (No. QNXZ2018034)。
文摘OBJECTIVE: To evaluate the effects of moxa-burning heat stimulating acupoints Zusanli(ST36) and Shenshu(BL23) on macrophage migration inhibitory factor(MIF) and its related molecules which can provide scientific experimental basis for the clinical application of moxibustion treatment of rheumatoid arthritis(RA). METHODS: Thirty rabbits were randomly assigned to control group, RA model(established by injecting Freund’s Complete Adjuvant) group(RA group) and RA model with moxibustion group [Moxa group, Zusanli(ST36) and Shenshu(BL23), 5 moxa pillars/day, 6 d × 3]. The expressions of MIF m RNA were evaluated with reverse transcription polymerase chain reaction;the apoptosis rates of macrophages were detected by erminal deoxynucleotidyl transferase-mediated d TUP nick end labeling;the expressions of related signal molecules were detected with immunohistochemical S-P method and the levels of IL-2 were detected with enzymelinked immunosorbent assay. RESULTS: The expressions of MIF m RNA, extracellular regulated protein kinases 2, p38 mitogen-activated protein kinase and nuclear factor-κ-gene binding p65 in synovial tissue of RA group were significantly increased when compared with control group, which were lower remarkably in moxa group than those in RA group. The apoptosis rates of macrophages in RA group were significantly down-regulated as compared with the control group, which were up-regulated in moxa group compared with the RA group. The levels of IL-2 in synovial fluid from the RA group were elevated significantly as compared with that from control group, but those of the moxa group were reduced when compared with those from RA group. CONCLUSIONS: Moxibustion may simultaneously regulate the expressions of MIF and its related signaling pathways molecules, the apoptosis rate of macrophages in synovial tissue, as well as the level of inflammatory factors in synovial fluid. The results suggest that the antiinflammatory effect of moxibustion on RA may be related to inhibit the expression of MIF in synovial tissue, the molecules of some related signaling pathways and promote the apoptosis of macrophage.
