The objectives of this study were to investigate the effects of red and blue LEDs on in vitro growth and microtuberization of potato(Solanum tuberosum) singlenode cuttings. Explants were incubated under 6 light treatm...The objectives of this study were to investigate the effects of red and blue LEDs on in vitro growth and microtuberization of potato(Solanum tuberosum) singlenode cuttings. Explants were incubated under 6 light treatments: 100% red LEDs(R), 75% red LEDs + 25%blue LEDs(3 RB), 50% red LEDs + 50% blue LEDs(RB),25% red LEDs + 75% blue LEDs(R3 B), 100% blue LEDs(B) and white LEDs(W). Most of the growth and physiological parameters were significantly higher in3 RB than W. Enhancement of leaf area and chlorophyll concentrations were obtained in B. Leaf stomata were elliptical with the lowest density in 3 RB. However, those in W were round in shape, and those with the smallest size and the highest density were observed in R. Most of the characteristics of microtuberization were also improved in3 RB. The combined spectra of red and blue LEDs increased the number of large microtubers. The fresh weight of individual microtubers in R and W were increased, but not their number. These results suggest that, of the treatments assessed, 3 RB is optimal for the in vitro growth of potato plantlets and the combination of red and blue LEDs is beneficial for microtuberization.展开更多
[Objective] This research aimed to solve the problems of growth and differentiation inhibition of transgenic potato plants caused by antibiotics used for bacteriostasis. [Method] Microtubers were induced using transge...[Objective] This research aimed to solve the problems of growth and differentiation inhibition of transgenic potato plants caused by antibiotics used for bacteriostasis. [Method] Microtubers were induced using transgenic potato plants, which had generated shoots and formed transgenic bacteria-free plants. [Result] Among the three transgenic potato varieties, the optimal induction medium for SⅠ and SⅡ were MS＋ 0.5 mg/L of 6-BA ＋ 0.1 mg/L of GA3＋ 150 mg/L of cef, and the optimal induction medium for NT were MS＋ 0.5 mg/L of ZT ＋ 0.1 mg/L of GA3 ＋ 150 mg/L of cef; the optimal differentiation medium for tubers were MS＋ 0.5 mg/L of ZT ＋ 0.1 mg/L of NAA, and the tubers with diameters ranging from 0.5 to 0.7 cm had generated the most shoots. The transgenic bacteria-free plants were cultivated in propagation medium without antibiotics for 30 d with a contamination rate of 0, and the stems of bacteria-free plants were stout with no branching. [Conclusion] This method is simple and could be easily applied for the removal of bacteria, which had cleared away obstacles for the selection and growth of transgenic individuals.展开更多
The potato minitubers have been widely used for the elite seed propagation to improve the seed potato system in China. However, little information is available for an efficient production of the minitubers with high p...The potato minitubers have been widely used for the elite seed propagation to improve the seed potato system in China. However, little information is available for an efficient production of the minitubers with high plant density in the protected growing conditions like plastic houses. In present research, the minitubers of a wide-grown potato variety, Favorita, were produced with the microtubers from tissue culture and the tip-cuttings of the microtuber plants. Three plant densities, 200, 400 and 600 microtubers or plants m-2 were set up with the randomized block design of 3 replications and the experiment was repeated in 4 seasons in 2009-2010. The canopy development, light interception, dry weight production and partitioning, tuberization and tuber weight were investigated to elucidate the mechanism by which the plant density affects the formation and growth of the minitubers. The results showed that the number of the tubers formed per unite area was in line with the increase in plant density. The difference in leaf area index （LAI） between the plant densities, especially in early stage of the plant growth, resulted in more radiation interception and dry weight producing in higher plant density than in lower one. However, our analysis demonstrated that the conversion coefficient of the cumulative intercepted radiation to plant weight and the dry weight partition rate to the tubers were constant between plant densities, suggesting that less amount of the photoassimilates partitioned to individual tubers is causal for more small tubers in high plant density. A negative exponential curve model, determined by total number of tubers produced per unit area and the mean tuber weight, fitted well to the tuber size distribution pattern. The optimum plant density could be estimated from this model for a maximum production of the minitubers with desired size.展开更多
[ Objective] This study aimed to investigate the variation rule of total RNA concentration during formation of microtubers of PineUia ternata induced by sucrose. [ Method ] Plantlets of P. ternata were used as experim...[ Objective] This study aimed to investigate the variation rule of total RNA concentration during formation of microtubers of PineUia ternata induced by sucrose. [ Method ] Plantlets of P. ternata were used as experimental materials. Total RNA concentration was determined with spectrophotometer, and the variation of total RNA was analyzed before and after formation of P. ternata microtubers treated with different concentrations of sucrose using DDRT-PCR technology. [ Result] Total RNA concentration of P. ternata treated with different concentrations of sucrose varied greatly, which showed an upward trend in the first 15 d, de- clined from 15~ d to 25th d, and slightly raised from 25＇h d to 30＇8 d. Microtubers formed under various sucrose treatments showed an increase in RNA concentration after induction, and it was assumed that the treatment of high concentrations of sucrose might have activated the expression of development-related＇ genes in P. ter- nata microtubers. [ Conclusion] This study provides the basis for revealing the related gene expression information and molecular mechanism during formation of P. ternata microtubers induced by sucrose.展开更多
It is the simplest,fastest and effective way to improve the yield level of potato by selecting high quality virus-free seed potato for field production and maintaining the original characters of virus-free minituber. ...It is the simplest,fastest and effective way to improve the yield level of potato by selecting high quality virus-free seed potato for field production and maintaining the original characters of virus-free minituber. Microtuber is featured by small volume,no pathogen,convenient storage and transportation,which can be induced in summer with high temperature and humidity,and overcome a series of problems such as poor survival rate of transplanted test-tube plantlets,and easy pollution of cutting tips. Therefore,it can replace conventional test-tube plantlets directly for reproduction. Meantime,if minitubers are sown ahead of time using cold frame,the growth stage of potato minituber will avoid the occurrence period of aphids. The use of gauze cover for insect prevention has good effect on maintaining the excellent characters of microtuber. The yield and quality of virus-free potato are greatly improved by means of virus-free culture of shoot tip seedlings,subculture propagation of virus-free seedlings,induction of microtubers by dark culture,cultivation of breeder’s seeds in greenhouse and screenhouse to preserve the characters of potato.展开更多
The production of atificial seeds by encapsulating microtubers of potato included a series of processes. Initially, the micropropagation of adventitious buds and microtuber - inducing system should be established. Bot...The production of atificial seeds by encapsulating microtubers of potato included a series of processes. Initially, the micropropagation of adventitious buds and microtuber - inducing system should be established. Both high quality microtuber and synchronizing microtuber which have the same size were necessary for producing health potato artificial seeds. In our experiment, the optimum medium of adventitious shoot-inducing of potato Hutao, Kesi, and Favorita is DCR + 1.0 mg / kg BA+ 0.5 mg / kg IBA+ 500 mg/ kg LH; the optium medium of microtuber- inducing of Hutao and Kesi is DCR+5.0 mg/ kg BA+200 mg/ kg Lh+8% sugar, and the optium medium of microtuber -inducing of Favorita is DCR+ 5.0 mg/ kg BA+0.1 mg / kg NAA+200 mg/ kg LH+8% sugar. Microtubers of the same size were gained by selecting the synchronous microtubers. 2.0% sodium alginate was used to encapsulate the microtubers of potato to form the artificial seeds, and the concentration of Ca C12 is 4%. Phytohormone NAA promoted the germination of potato artificial seeds. These artificial seeds have more than 90% germinating frequency in plant hormone-free DCR medium.展开更多
基金supported by the National High Technology Research and Development Program of China (2013 AA 103005)the Natural Science Foundation of Beijing (6144022)
文摘The objectives of this study were to investigate the effects of red and blue LEDs on in vitro growth and microtuberization of potato(Solanum tuberosum) singlenode cuttings. Explants were incubated under 6 light treatments: 100% red LEDs(R), 75% red LEDs + 25%blue LEDs(3 RB), 50% red LEDs + 50% blue LEDs(RB),25% red LEDs + 75% blue LEDs(R3 B), 100% blue LEDs(B) and white LEDs(W). Most of the growth and physiological parameters were significantly higher in3 RB than W. Enhancement of leaf area and chlorophyll concentrations were obtained in B. Leaf stomata were elliptical with the lowest density in 3 RB. However, those in W were round in shape, and those with the smallest size and the highest density were observed in R. Most of the characteristics of microtuberization were also improved in3 RB. The combined spectra of red and blue LEDs increased the number of large microtubers. The fresh weight of individual microtubers in R and W were increased, but not their number. These results suggest that, of the treatments assessed, 3 RB is optimal for the in vitro growth of potato plantlets and the combination of red and blue LEDs is beneficial for microtuberization.
基金Supported by the Open Project of National Engineering Laboratory for Tree Breeding of Beijing Forestry University~~
文摘[Objective] This research aimed to solve the problems of growth and differentiation inhibition of transgenic potato plants caused by antibiotics used for bacteriostasis. [Method] Microtubers were induced using transgenic potato plants, which had generated shoots and formed transgenic bacteria-free plants. [Result] Among the three transgenic potato varieties, the optimal induction medium for SⅠ and SⅡ were MS＋ 0.5 mg/L of 6-BA ＋ 0.1 mg/L of GA3＋ 150 mg/L of cef, and the optimal induction medium for NT were MS＋ 0.5 mg/L of ZT ＋ 0.1 mg/L of GA3 ＋ 150 mg/L of cef; the optimal differentiation medium for tubers were MS＋ 0.5 mg/L of ZT ＋ 0.1 mg/L of NAA, and the tubers with diameters ranging from 0.5 to 0.7 cm had generated the most shoots. The transgenic bacteria-free plants were cultivated in propagation medium without antibiotics for 30 d with a contamination rate of 0, and the stems of bacteria-free plants were stout with no branching. [Conclusion] This method is simple and could be easily applied for the removal of bacteria, which had cleared away obstacles for the selection and growth of transgenic individuals.
基金supported by Earmarked Fund for Morden Agro-industry Technology Research System(CARS-10-P08)
文摘The potato minitubers have been widely used for the elite seed propagation to improve the seed potato system in China. However, little information is available for an efficient production of the minitubers with high plant density in the protected growing conditions like plastic houses. In present research, the minitubers of a wide-grown potato variety, Favorita, were produced with the microtubers from tissue culture and the tip-cuttings of the microtuber plants. Three plant densities, 200, 400 and 600 microtubers or plants m-2 were set up with the randomized block design of 3 replications and the experiment was repeated in 4 seasons in 2009-2010. The canopy development, light interception, dry weight production and partitioning, tuberization and tuber weight were investigated to elucidate the mechanism by which the plant density affects the formation and growth of the minitubers. The results showed that the number of the tubers formed per unite area was in line with the increase in plant density. The difference in leaf area index （LAI） between the plant densities, especially in early stage of the plant growth, resulted in more radiation interception and dry weight producing in higher plant density than in lower one. However, our analysis demonstrated that the conversion coefficient of the cumulative intercepted radiation to plant weight and the dry weight partition rate to the tubers were constant between plant densities, suggesting that less amount of the photoassimilates partitioned to individual tubers is causal for more small tubers in high plant density. A negative exponential curve model, determined by total number of tubers produced per unit area and the mean tuber weight, fitted well to the tuber size distribution pattern. The optimum plant density could be estimated from this model for a maximum production of the minitubers with desired size.
基金Supported by National Natural Science Foundation of China (30973963)Anhui Provincial Natural Science Foundation (090413252)Key Program of Natural Science Research of Anhui Provincial Universities (KJ2009A160)
文摘[ Objective] This study aimed to investigate the variation rule of total RNA concentration during formation of microtubers of PineUia ternata induced by sucrose. [ Method ] Plantlets of P. ternata were used as experimental materials. Total RNA concentration was determined with spectrophotometer, and the variation of total RNA was analyzed before and after formation of P. ternata microtubers treated with different concentrations of sucrose using DDRT-PCR technology. [ Result] Total RNA concentration of P. ternata treated with different concentrations of sucrose varied greatly, which showed an upward trend in the first 15 d, de- clined from 15~ d to 25th d, and slightly raised from 25＇h d to 30＇8 d. Microtubers formed under various sucrose treatments showed an increase in RNA concentration after induction, and it was assumed that the treatment of high concentrations of sucrose might have activated the expression of development-related＇ genes in P. ter- nata microtubers. [ Conclusion] This study provides the basis for revealing the related gene expression information and molecular mechanism during formation of P. ternata microtubers induced by sucrose.
基金Supported by Special Project of Potato Industry Research System in Shandong Province (SDAIT-14)。
文摘It is the simplest,fastest and effective way to improve the yield level of potato by selecting high quality virus-free seed potato for field production and maintaining the original characters of virus-free minituber. Microtuber is featured by small volume,no pathogen,convenient storage and transportation,which can be induced in summer with high temperature and humidity,and overcome a series of problems such as poor survival rate of transplanted test-tube plantlets,and easy pollution of cutting tips. Therefore,it can replace conventional test-tube plantlets directly for reproduction. Meantime,if minitubers are sown ahead of time using cold frame,the growth stage of potato minituber will avoid the occurrence period of aphids. The use of gauze cover for insect prevention has good effect on maintaining the excellent characters of microtuber. The yield and quality of virus-free potato are greatly improved by means of virus-free culture of shoot tip seedlings,subculture propagation of virus-free seedlings,induction of microtubers by dark culture,cultivation of breeder’s seeds in greenhouse and screenhouse to preserve the characters of potato.
文摘The production of atificial seeds by encapsulating microtubers of potato included a series of processes. Initially, the micropropagation of adventitious buds and microtuber - inducing system should be established. Both high quality microtuber and synchronizing microtuber which have the same size were necessary for producing health potato artificial seeds. In our experiment, the optimum medium of adventitious shoot-inducing of potato Hutao, Kesi, and Favorita is DCR + 1.0 mg / kg BA+ 0.5 mg / kg IBA+ 500 mg/ kg LH; the optium medium of microtuber- inducing of Hutao and Kesi is DCR+5.0 mg/ kg BA+200 mg/ kg Lh+8% sugar, and the optium medium of microtuber -inducing of Favorita is DCR+ 5.0 mg/ kg BA+0.1 mg / kg NAA+200 mg/ kg LH+8% sugar. Microtubers of the same size were gained by selecting the synchronous microtubers. 2.0% sodium alginate was used to encapsulate the microtubers of potato to form the artificial seeds, and the concentration of Ca C12 is 4%. Phytohormone NAA promoted the germination of potato artificial seeds. These artificial seeds have more than 90% germinating frequency in plant hormone-free DCR medium.
