【目的】探究温拌沥青混合料黏附机理,拓展温拌沥青与集料黏附性研究方法。【方法】使用原子力显微镜(atomic force microscope,AFM)测得分别加入Sasobit类温拌剂H01(质量分数分别为1.0%、3.0%、5.0%)和表面活性剂类温拌剂H02(质量分数...【目的】探究温拌沥青混合料黏附机理,拓展温拌沥青与集料黏附性研究方法。【方法】使用原子力显微镜(atomic force microscope,AFM)测得分别加入Sasobit类温拌剂H01(质量分数分别为1.0%、3.0%、5.0%)和表面活性剂类温拌剂H02(质量分数分别为0.3%、0.5%、0.7%)的温拌沥青试样黏附力,并经JKR(Johnson-Kendall-Roberts)模型和表面能理论将其转换为沥青表面能,计算沥青-花岗岩和沥青-玄武岩体系的无水、有水黏附功,并与水煮试验、水稳定性试验结果进行对比分析;对基于AFM测得的温拌沥青退针力曲线进行积分,得到黏附性指标G,并将其与沥青表面能进行相关性拟合。【结果】两类温拌剂的加入均降低了温拌沥青-集料体系的黏附功,温拌沥青-花岗岩体系的无水黏附功比温拌沥青-玄武岩体系的大,有水黏附功则相反;基于AFM求解得到的H01温拌沥青-玄武岩体系黏附功的变化趋势与水稳定性试验结果相同,而H02温拌沥青-玄武岩体系的则相反;黏附性指标G与沥青表面能之间具有较好的相关性。【结论】相比温拌沥青-玄武岩体系,温拌沥青-花岗岩体系更容易发生水损害;基于AFM求解沥青-玄武岩体系黏附功的方法适用于Sasobit类温拌沥青,不适用于表面活性剂类温拌沥青;本文提出的黏附性指标G为评价温拌沥青-集料体系黏附性提供了新的量化指标。展开更多
Objective:Verrucous epidermal nevus(VEN),seborrheic keratosis(SK),verruca plana(VP),verruca vulgaris(VV),and nevus sebaceous(NS)are common verrucous proliferative skin diseases with similar clinical appearances,often ...Objective:Verrucous epidermal nevus(VEN),seborrheic keratosis(SK),verruca plana(VP),verruca vulgaris(VV),and nevus sebaceous(NS)are common verrucous proliferative skin diseases with similar clinical appearances,often posing diagnostic challenges.Dermoscopy and reflectance confocal microscopy(RCM)can aid in their differentiation,yet their specific features under these tools have not been systematically described.This study aims to summarize and analyze the dermoscopic and RCM features of VEN,SK,VP,VV,and NS.Methods:A total of 121 patients with histopathologically confirmed verrucous proliferative skin diseases were enrolled.Dermoscopy and RCM imaging was used to observe and analyze the microscopic features of these conditions.Results:Under dermoscopy,the 5 diseases displayed distinct characteristics:VEN typically showed gyriform structures;SK was characterized by gyriform structures,comedo-like openings,and milia-like cysts;VP and VV featured dotted vessels and frogspawn-like structures;NS presented as brownish-yellow globules.RCM revealed shared features such as hyperkeratosis and acanthosis across all 5 diseases.Specific features included gyriform structures and elongated rete ridges in VEN;pseudocysts and gyriform structures in SK;evenly distributed ring-like structures in VP;vacuolated cells and papillomatous proliferation in VV;and frogspawn-like structures in NS.Conclusion:These 5 verrucous proliferative skin conditions exhibit distinguishable features under both dermoscopy and RCM.The combination of these 2 noninvasive imaging modalities holds significant clinical value for the differential diagnosis of verrucous proliferative skin diseases.展开更多
Recently,charged solitons have been found in a two-dimensional CoCl_(2)/HOPG system,whose microscopic nature remains to be elusive.In this work,we investigate the charged solitons in monolayer CoCl_(2) using scanning ...Recently,charged solitons have been found in a two-dimensional CoCl_(2)/HOPG system,whose microscopic nature remains to be elusive.In this work,we investigate the charged solitons in monolayer CoCl_(2) using scanning tunneling microscopy(STM)and atomic force microscopy(AFM).Moreover,we study the electrical properties of the charged solitons at zero electric field by measuring local contact potential difference(LCPD)via Kelvin probe force microscopy(KPFM)using the Δf(V)method.The compensation voltage corresponding to the vertex of the parabola is obtained by fitting the quadratic relationship between Δf and sample bias.The results show that,without an external electric field,the solitons behave as negatively charged entities.Meanwhile,the LCPD mapping characterizes the spatial distribution of the potential at the charged solitons,which agrees well with those obtained from STM band bending measurements.展开更多
Full-color imaging is essential in digital pathology for accurate tissue analysis.Utilizing advanced optical modulation and phase retrieval algorithms,Fourier ptychographic microscopy(FPM)offers a powerful solution fo...Full-color imaging is essential in digital pathology for accurate tissue analysis.Utilizing advanced optical modulation and phase retrieval algorithms,Fourier ptychographic microscopy(FPM)offers a powerful solution for high-throughput digital pathology,combining high resolution,large field of view,and extended depth of field(DOF).However,the full-color capabilities of FPM are hindered by coherent color artifacts and reduced computational efficiency,which significantly limits its practical applications.Color-transferbased FPM(CFPM)has emerged as a potential solution,theoretically reducing both acquisition and reconstruction threefold time.Yet,existing methods fall short of achieving the desired reconstruction speed and colorization quality.In this study,we report a generalized dual-color-space constrained model for FPM colorization.This model provides a mathematical framework for model-based FPM colorization,enabling a closed-form solution without the need for redundant iterative calculations.Our approach,termed generalized CFPM(gCFPM),achieves colorization within seconds for megapixel-scale images,delivering superior colorization quality in terms of both colorfulness and sharpness,along with an extended DOF.Both simulations and experiments demonstrate that gCFPM surpasses state-of-the-art methods across all evaluated criteria.Our work offers a robust and comprehensive workflow for high-throughput full-color pathological imaging using FPM platforms,laying a solid foundation for future advancements in methodology and engineering.展开更多
The characteristics of nonmetallic inclusions formed during steel production have a significant influence on steel performance.In this paper,studies on inclusions using confocal scanning laser microscopy(CSLM)are revi...The characteristics of nonmetallic inclusions formed during steel production have a significant influence on steel performance.In this paper,studies on inclusions using confocal scanning laser microscopy(CSLM)are reviewed and summarized,particularly the col-lision of various inclusions,dissolution of inclusions in liquid slag,and reactions between inclusions and steel.Solid inclusions exhibited a high collision tendency,whereas pure liquid inclusions exhibited minimal collisions because of the small attraction force induced by their<90°contact angle with molten steel.The collision of complex inclusions in molten steel was not included in the scope of this study and should be evaluated in future studies.Higher CaO/Al_(2)O_(3)and CaO/SiO_(2)ratios in liquid slag promoted the dissolution of Al_(2)O_(3)-based in-clusions.The formation of solid phases in the slag should be prevented to improve dissolution of inclusions.To accurately simulate the dissolution of inclusions in liquid slag,in-situ observation of the dissolution of inclusions at the steel-slag interface is necessary.Using a combination of CSLM and scanning electron microscopy-energy dispersive spectroscopy,the composition and morphological evolution of the inclusions during their modification by the dissolved elements in steel were observed and analyzed.Although the in-situ observa-tion of MnS and TiN precipitations has been widely studied,the in-situ observation of the evolution of oxide inclusions in steel during so-lidification and heating processes has rarely been reported.The effects of temperature,heating and cooling rates,and inclusion character-istics on the formation of acicular ferrites(AFs)have been widely studied.At a cooling rate of 3-5 K/s,the order of AF growth rate in-duced by different inclusions,as reported in literature,is Ti-O<Ti-Ca-Zr-Al-O<Mg-O<Ti-Zr-Al-O<Mn-Ti-Al-O<Ti-Al-O<Zr-Ti-Al-O.Further comprehensive experiments are required to investigate the quantitative relationship between the formation of AFs and inclusions.展开更多
High-resolution transmission electron microscopy(HRTEM)promises rapid atomic-scale dynamic structure imaging.Yet,the precision limitations of aberration parameters and the challenge of eliminating aberrations in Cs-co...High-resolution transmission electron microscopy(HRTEM)promises rapid atomic-scale dynamic structure imaging.Yet,the precision limitations of aberration parameters and the challenge of eliminating aberrations in Cs-corrected transmission electron microscopy constrain resolution.A machine learning algorithm is developed to determine the aberration parameters with higher precision from small,lattice-periodic crystal images.The proposed algorithm is then validated with simulated HRTEM images of graphene and applied to the experimental images of a molybdenum disulfide(MoS_(2))monolayer with 25 variables(14 aberrations)resolved in wide ranges.Using these measured parameters,the phases of the exit-wave functions are reconstructed for each image in a focal series of MoS_(2)monolayers.The images were acquired due to the unexpected movement of the specimen holder.Four-dimensional data extraction reveals time-varying atomic structures and ripple.In particular,the atomic evolution of the sulfur-vacancy point and line defects,as well as the edge structure near the amorphous,is visualized as the resolution has been improved from about 1.75?to 0.9 A.This method can help salvage important transmission electron microscope images and is beneficial for the images obtained from electron microscopes with average stability.展开更多
To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral cortex.However,these me...To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral cortex.However,these methods often require complex systems and the effect of age on cerebral embolism has not been adequately studied,although ischemic stroke is strongly age-related.In this study,we propose an optical-resolution photoacoustic microscopy-based visualized photothrombosis methodology to create and monitor ischemic stroke in mice simultaneously using a 532 nm pulsed laser.We observed the molding process in mice of different ages and presented age-dependent vascular embolism differentiation.Moreover,we integrated optical coherence tomography angiography to investigate age-associated trends in cerebrovascular variability following a stroke.Our imaging data and quantitative analyses underscore the differential cerebrovascular responses to stroke in mice of different ages,thereby highlighting the technique's potential for evaluating cerebrovascular health and unraveling age-related mechanisms involved in ischemic strokes.展开更多
Stochastic optical reconstruction microscopy(STORM),as a typical technique of single-molecule localization microscopy(SMLM),has overcome the diffraction limit by randomly switching fluorophores between fluorescent and...Stochastic optical reconstruction microscopy(STORM),as a typical technique of single-molecule localization microscopy(SMLM),has overcome the diffraction limit by randomly switching fluorophores between fluorescent and dark states,allowing for the precise localization of isolated emission patterns and the super-resolution reconstruction from millions of localized positions of single fluorophores.A critical factor influencing localization precision is the photo-switching behavior of fluorophores,which is affected by the imaging buffer.The imaging buffer typically comprises oxygen scavengers,photo-switching reagents,and refractive index regulators.Oxygen scavengers help prevent photobleaching,photo-switching reagents assist in facilitating the conversion of fluorophores,and refractive index regulators are used to adjust the refractive index of the solution.The synergistic interaction of these components promotes stable blinking of fluorophores,reduces irreversible photobleaching,and thereby ensures high-quality super-resolution imaging.This review provides a comprehensive overview of the essential compositions and functionalities of imaging buffers used in STORM,serving as a valuable resource for researchers seeking to select appropriate imaging buffers for their experiments.展开更多
Background:Glioblastoma(GBM)is a highly aggressive brain tumor characterized by aberrant angiogenesis and an immunosuppressive microenvironment.Pericytes are aberrantly recruited but their spatiotemporal roles and mol...Background:Glioblastoma(GBM)is a highly aggressive brain tumor characterized by aberrant angiogenesis and an immunosuppressive microenvironment.Pericytes are aberrantly recruited but their spatiotemporal roles and molecular changes remain unclear.This study investigated platelet-derived growth factor receptor beta-positive(Pdgfrb+)pericyte dynamics and reprogramming in GBM vasculature.Methods:We generated GL261-Luc and GL261-CFP glioblastoma cells via lentiviral transduction and established two transgenic models.(1)For pericyte labeling,Ai14 reporter mice was crossed with PDGFRβ-P2A-CreERT2mice for td Tomato-specific lineage tracing(PT mice).(2)For conditional ablation,we generated inducible Pdgfrb-expressing cell ablation models(PT mice was crossed with ROSA-DTA mice).An intravital imaging platform(FITC-dextran/CFP/td Tomato+two-photon microscopy)tracked pericytes,vessels,and tumor cells,while FACSsorted Pdgfrb+cells from GBM and normal brain were analyzed by LC-MS/MS proteomics.Results:Cre-mediated ablation of Pdgfrb-expressing cells revealed stage-dependent effects on GBM growth:early ablation inhibited progression while late ablation promoted it.Pericytes undergo dual spatial reorganization in GBM:regional enrichment with pre-sprouting accumulation at the tumor-brain interface,and focal positioning with preferential localization at vascular branch points.Concurrently,GBM vasculature displayed simplified branching,dilation,and pericyte remodeling(shorter processes,higher density).Proteomics revealed 1426 altered proteins,with upregulated proliferation pathways(e.g.,matrix metallopeptidase 14[Mmp14],lysyl oxidase like 2[Loxl2])and downregulated homeostasis functions(e.g.,transforming growth factor beta 1[Tgfb1]),validated by scRNA-seq in human GBM.Conclusions:This study demonstrates that during early GBM progression,pericytes actively drive tumor angiogenesis through molecular reprogramming toward proliferative and pro-angiogenic phenotypes,with the integrated imaging-proteomics framework revealing potential therapeutic targets for disrupting pericyte-mediated vascular remodeling.展开更多
Maintaining the s-polarization state of laser beams is important to achieve high modulation depth in a laser-interference-based super-resolution structured illumination microscope(SR-SIM).However,the imperfect optical...Maintaining the s-polarization state of laser beams is important to achieve high modulation depth in a laser-interference-based super-resolution structured illumination microscope(SR-SIM).However,the imperfect optical components can depolarize the laser beams hence degenerating the modulation depth.Here,we first presented a direct measurement method designed to estimate the modulation depth more precisely by shifting illumination patterns with equal phase steps.This measurement method greatly reduces the dependence of modulation depths on the samples,and then developed a polarization optimization method to achieve high modulation depth at all orientations by actively and quantitatively compensating for the additional phase difference using a combination of waveplate and a liquid crystal variable retarder(LCVR).Experimental results demonstrate that our method can achieve illumination patterns with modulation depth higher than 0.94 at three orientations with only one LCVR voltage,which enables isotropic resolution improvement.展开更多
Holographic microscopy has emerged as a vital tool in biomedicine,enabling visualization of microscopic morphological features of tissues and cells in a label-free manner.Recently,deep learning(DL)-based image reconst...Holographic microscopy has emerged as a vital tool in biomedicine,enabling visualization of microscopic morphological features of tissues and cells in a label-free manner.Recently,deep learning(DL)-based image reconstruction models have demonstrated state-of-the-art performance in holographic image reconstruction.However,their utility in practice is still severely limited,as conventional training schemes could not properly handle out-of-distribution data.Here,we leverage backpropagation operation and reparameterization of the forward propagator to enable an adaptable image reconstruction model for histopathologic inspection.Only given with a training dataset of rectum tissue images captured from a single imaging configuration,our scheme consistently shows high reconstruction performance even with the input hologram of diverse tissue types at different pathological states captured under various imaging configurations.Using the proposed adaptation technique,we show that the diagnostic features of cancerous colorectal tissues,such as dirty necrosis,captured with 5×magnification and a numerical aperture(NA)of 0.1,can be reconstructed with high accuracy,whereas a given training dataset is strictly confined to normal rectum tissues acquired under the imaging configuration of 20×magnification and an NA of 0.4.Our results suggest that the DL-based image reconstruction approaches,with sophisticated adaptation techniques,could offer an extensively generalizable solution for inverse mapping problems in imaging.展开更多
The assembly behaviors of two low-symmetric carboxylic acid molecules(50-(6-carboxynaphthalen-2-yl)-[1,10:30,100-triphenyl]-3,400,5-tricarboxylic acid(CTTA)and 30,50-bis(6-carboxynaphthalen-2-yl)-[1,10-biphenyl]-3,5-d...The assembly behaviors of two low-symmetric carboxylic acid molecules(50-(6-carboxynaphthalen-2-yl)-[1,10:30,100-triphenyl]-3,400,5-tricarboxylic acid(CTTA)and 30,50-bis(6-carboxynaphthalen-2-yl)-[1,10-biphenyl]-3,5-dicarboxylic acid(BCBDA))containing naphthalene rings on graphite surfaces have been investigated using scanning tunneling microscopy(STM).The transformation of nanostructures induced by the second components(EDA and PEBP-C4)have been also examined.Both CTTA and BCBDA molecules self-assemble at the 1-heptanoic acid(HA)/HOPG interface,forming porous network structures.The dimer represents the most elementary building unit due to the formation of double hydrogen bonds.Moreover,the flipping of naphthalene ring results in the isomerization of BCBDA molecule.The introduction of carboxylic acid derivative EDA disrupts the dimer,which subsequently undergoes a structural conformation to form a novel porous structure.Furthermore,upon the addition of pyridine derivative PEBP-C4,N–H⋯O hydrogen bonds are the dominant forces driving the three coassembled structures.We have also conducted density functional theory(DFT)calculations to determine the molecular conformation and analyze the mechanisms underlying the formation of nanostructures.展开更多
The advancement of electron microscopy technology has driven the development of electron microscopes that can apply mechanical loading while observing samples,providing a valuable tool for In-Situ mechanical character...The advancement of electron microscopy technology has driven the development of electron microscopes that can apply mechanical loading while observing samples,providing a valuable tool for In-Situ mechanical characterization of materials.In response to the need to characterize the evolution of the mechanical behavior of structural materials,such as aerospace materials,in real cryogenic service environments,and to provide an experimental basis for improving their macroscopic cryogenic mechanical properties,the advancement of In-Situ characterization techniques capable of offering both cryogenic environments and mechanical loading has become imperative.There have been scholars using this technique to carry out cryogenic mechanical In-Situ studies of related materials,with experimental studies dominating in general,and a few reviews of mechanical characterization techniques mentioning cryogenic temperatures.In order to make it easier to conduct research using such characterization techniques and to further promote the development of related characterization techniques,this review compiles the previous work and summarizes the electron microscope-based In-Situ characterization techniques for cryogenic micro-and nanomechanics.These techniques primarily include transmission electron microscopy-based cryogenic tensile and indentation methods,as well as scanning electron microscopy-based cryogenic tensile,indentation,compression,and bending methods.Furthermore,the review outlines the prospective future development of In-Situ characterization techniques for cryogenic micro-and nanomechanics.展开更多
Deep learning(DL)is making significant inroads into biomedical imaging as it provides novel and powerful ways of accurately and efficiently improving the image quality of photoacoustic microscopy(PAM).Off-the-shelf DL...Deep learning(DL)is making significant inroads into biomedical imaging as it provides novel and powerful ways of accurately and efficiently improving the image quality of photoacoustic microscopy(PAM).Off-the-shelf DL models,however,do not necessarily obey the fundamental governing laws of PAM physical systems,nor do they generalize well to scenarios on which they have not been trained.In this work,a physics-embedded degeneration learning(PEDL)approach is proposed to enhance the image quality of PAM with a self-attention enhanced U-Net network,which obtains greater physical consistency,improves data efficiency,and higher adaptability.The proposed method is demonstrated on both synthetic and real datasets,including animal experiments in vivo(blood vessels of mouse's ear and brain).And the results show that compared with previous DL methods,the PEDL algorithm exhibits good performance in recovering PAM images qualitatively and quantitatively.It overcomes the challenges related to training data,accuracy,and robustness which a typical data-driven approach encounters,whose exemplary application envisions to provide a new perspective for existing DL tools of enhanced PAM.展开更多
Microsphere and microcylinder-assisted microscopy(MAM)has grown steadily over the last decade and is still an intensively studied optical far-field imaging technique that promises to overcome the fundamental lateral r...Microsphere and microcylinder-assisted microscopy(MAM)has grown steadily over the last decade and is still an intensively studied optical far-field imaging technique that promises to overcome the fundamental lateral resolution limit of microscopy.However,the physical effects leading to resolution enhancement are still frequently debated.In addition,various configurations of MAM operating in transmission mode as well as reflection mode are examined,and the results are sometimes generalized.We present a rigorous simulation model of MAM and introduce a way to quantify the resolution enhancement.The lateral resolution is compared for microscope arrangements in reflection and transmission modes.Furthermore,we discuss different physical effects with respect to their contribution to resolution enhancement.The results indicate that the effects impacting the resolution in MAM strongly depend on the arrangement of the microscope and the measurement object.As a highlight,we outline that evanescent waves in combination with whispering gallery modes also improve the imaging capabilities,enabling super-resolution under certain circumstances.This result is contrary to the conclusions drawn from previous studies,where phase objects have been analyzed,and thus further emphasizes the complexity of the physical mechanisms underlying MAM.展开更多
High-temperature confocal scanning laser microscopy(HT-CSLM)is a potent methodology for investigating various phenomena in the field of metallurgy.Initially applied to the observation of solid phase transformations an...High-temperature confocal scanning laser microscopy(HT-CSLM)is a potent methodology for investigating various phenomena in the field of metallurgy.Initially applied to the observation of solid phase transformations and solidification,this method has gained traction in the field of non-metallic inclusion in steels in recent years.An overview of the experimental capabilities of HT-CSLM and the most important results of recent investigations regarding the topics of clean steel production are provided.It includes the formation of intragranular acicular ferrite(IAF)from the surface of non-metallic inclusions during the continuous cooling and heat treatment,which can be especially beneficial in the toughness of heat-affected zones of welded pieces.Furthermore,the investigation of agglomeration mechanisms of non-metallic inclusions(NMIs)in liquid steel is discussed to improve the insight into attraction forces between particles and clogging phenomena during continuous casting.Also,the dissolution of NMIs in various steelmaking slags can be observed by HT-CSLM to compare dissolution rates and mechanisms of NMI,where significant influences of temperature and chemical composition of the slag were shown.Last but not least,the experimental work regarding the interface between steel and slag is discussed,where novel techniques are currently being developed.A comprehensive summary of experimental techniques using HT-CSLM equipment to investigate different interactions of NMIs with steel and slag phases is compiled.展开更多
Traumatic penumbra(TP)is a region with recoverable potential around the primary lesion of brain injury.Rapid and accurate imaging for identifying TP is essential for treating traumatic brain injury(TBI).In this study,...Traumatic penumbra(TP)is a region with recoverable potential around the primary lesion of brain injury.Rapid and accurate imaging for identifying TP is essential for treating traumatic brain injury(TBI).In this study,we first established traumatic brain injuries(TBIs)in rats using a modified Feeney method,followed by label-free imaging of brain tissue sections with multiphoton fluorescence microscopy.The results showed that the technique effectively imaged normal and traumatic brain tissues,and revealed pathological features such as extracellular matrix changes,vascular cell proliferation,and intracellular edema in the traumatic penumbra.Compared with normal brain tissue,the extracellular matrix in the TP was sparse,cells were disorganized,and hyperplastic vascular cells emitted higher two-photon excited fluorescence(TPEF)signals.Our research demonstrates the potential of multiphoton fluorescence technology in the rapid diagnosis and therapeutic evaluation of TBI.展开更多
To eliminate distortion caused by vertical drift and illusory slopes in atomic force microscopy(AFM)imaging,a lifting-wavelet-based iterative thresholding correction method is proposed in this paper.This method achiev...To eliminate distortion caused by vertical drift and illusory slopes in atomic force microscopy(AFM)imaging,a lifting-wavelet-based iterative thresholding correction method is proposed in this paper.This method achieves high-quality AFM imaging via line-by-line corrections for each distorted profile along the fast axis.The key to this line-by-line correction is to accurately simulate the profile distortion of each scanning row.Therefore,a data preprocessing approach is first developed to roughly filter out most of the height data that impairs the accuracy of distortion modeling.This process is implemented through an internal double-screening mechanism.A line-fitting method is adopted to preliminarily screen out the obvious specimens.Lifting wavelet analysis is then carried out to identify the base parts that are mistakenly filtered out as specimens so as to preserve most of the base profiles and provide a good basis for further distortion modeling.Next,an iterative thresholding algorithm is developed to precisely simulate the profile distortion.By utilizing the roughly screened base profile,the optimal threshold,which is used to screen out the pure bases suitable for distortion modeling,is determined through iteration with a specified error rule.On this basis,the profile distortion is accurately modeled through line fitting on the finely screened base data,and the correction is implemented by subtracting the modeling result from the distorted profile.Finally,the effectiveness of the proposed method is verified through experiments and applications.展开更多
文摘【目的】探究温拌沥青混合料黏附机理,拓展温拌沥青与集料黏附性研究方法。【方法】使用原子力显微镜(atomic force microscope,AFM)测得分别加入Sasobit类温拌剂H01(质量分数分别为1.0%、3.0%、5.0%)和表面活性剂类温拌剂H02(质量分数分别为0.3%、0.5%、0.7%)的温拌沥青试样黏附力,并经JKR(Johnson-Kendall-Roberts)模型和表面能理论将其转换为沥青表面能,计算沥青-花岗岩和沥青-玄武岩体系的无水、有水黏附功,并与水煮试验、水稳定性试验结果进行对比分析;对基于AFM测得的温拌沥青退针力曲线进行积分,得到黏附性指标G,并将其与沥青表面能进行相关性拟合。【结果】两类温拌剂的加入均降低了温拌沥青-集料体系的黏附功,温拌沥青-花岗岩体系的无水黏附功比温拌沥青-玄武岩体系的大,有水黏附功则相反;基于AFM求解得到的H01温拌沥青-玄武岩体系黏附功的变化趋势与水稳定性试验结果相同,而H02温拌沥青-玄武岩体系的则相反;黏附性指标G与沥青表面能之间具有较好的相关性。【结论】相比温拌沥青-玄武岩体系,温拌沥青-花岗岩体系更容易发生水损害;基于AFM求解沥青-玄武岩体系黏附功的方法适用于Sasobit类温拌沥青,不适用于表面活性剂类温拌沥青;本文提出的黏附性指标G为评价温拌沥青-集料体系黏附性提供了新的量化指标。
基金supported by the Project of Health Committee of Hunan Province(D202304128868),China.
文摘Objective:Verrucous epidermal nevus(VEN),seborrheic keratosis(SK),verruca plana(VP),verruca vulgaris(VV),and nevus sebaceous(NS)are common verrucous proliferative skin diseases with similar clinical appearances,often posing diagnostic challenges.Dermoscopy and reflectance confocal microscopy(RCM)can aid in their differentiation,yet their specific features under these tools have not been systematically described.This study aims to summarize and analyze the dermoscopic and RCM features of VEN,SK,VP,VV,and NS.Methods:A total of 121 patients with histopathologically confirmed verrucous proliferative skin diseases were enrolled.Dermoscopy and RCM imaging was used to observe and analyze the microscopic features of these conditions.Results:Under dermoscopy,the 5 diseases displayed distinct characteristics:VEN typically showed gyriform structures;SK was characterized by gyriform structures,comedo-like openings,and milia-like cysts;VP and VV featured dotted vessels and frogspawn-like structures;NS presented as brownish-yellow globules.RCM revealed shared features such as hyperkeratosis and acanthosis across all 5 diseases.Specific features included gyriform structures and elongated rete ridges in VEN;pseudocysts and gyriform structures in SK;evenly distributed ring-like structures in VP;vacuolated cells and papillomatous proliferation in VV;and frogspawn-like structures in NS.Conclusion:These 5 verrucous proliferative skin conditions exhibit distinguishable features under both dermoscopy and RCM.The combination of these 2 noninvasive imaging modalities holds significant clinical value for the differential diagnosis of verrucous proliferative skin diseases.
基金supported by the National Key Research and Development Program of China(Grant Nos.2022YFA1403300 and 2019YFA0308404)the National Natural Science Foundation of China(Grant Nos.11427902,11991060,12074075,12474165,12274084,and 12241402)+5 种基金Shanghai Municipal Science and Technology Major Project(Grant No.2019SHZDZX01)Shanghai Municipal Natural Science Foundation(Grant No.22ZR1407400)Innovation Program for Quantum Science and Technology(Grant No.2024ZD0300104)Innovation Program of Shanghai Municipal Education Commission(Grant No.2023ZKZD03)Science and Technology Commission of Shanghai Municipality(Grant No.20JC1415900)China Postdoctoral Science Foundation(Grant No.KLH1512149).
文摘Recently,charged solitons have been found in a two-dimensional CoCl_(2)/HOPG system,whose microscopic nature remains to be elusive.In this work,we investigate the charged solitons in monolayer CoCl_(2) using scanning tunneling microscopy(STM)and atomic force microscopy(AFM).Moreover,we study the electrical properties of the charged solitons at zero electric field by measuring local contact potential difference(LCPD)via Kelvin probe force microscopy(KPFM)using the Δf(V)method.The compensation voltage corresponding to the vertex of the parabola is obtained by fitting the quadratic relationship between Δf and sample bias.The results show that,without an external electric field,the solitons behave as negatively charged entities.Meanwhile,the LCPD mapping characterizes the spatial distribution of the potential at the charged solitons,which agrees well with those obtained from STM band bending measurements.
基金supported by the National Natural Science Foundation of China(Grant Nos.12104500 and 82430062)the Key Research and Development Projects of Shaanxi Province(Grant No.2023-YBSF-263),the Shenzhen Engineering Research Centre(Grant No.XMHT20230115004)the Shenzhen Science and Technology Innovation Commission(Grant No.KCXFZ20201221173207022).
文摘Full-color imaging is essential in digital pathology for accurate tissue analysis.Utilizing advanced optical modulation and phase retrieval algorithms,Fourier ptychographic microscopy(FPM)offers a powerful solution for high-throughput digital pathology,combining high resolution,large field of view,and extended depth of field(DOF).However,the full-color capabilities of FPM are hindered by coherent color artifacts and reduced computational efficiency,which significantly limits its practical applications.Color-transferbased FPM(CFPM)has emerged as a potential solution,theoretically reducing both acquisition and reconstruction threefold time.Yet,existing methods fall short of achieving the desired reconstruction speed and colorization quality.In this study,we report a generalized dual-color-space constrained model for FPM colorization.This model provides a mathematical framework for model-based FPM colorization,enabling a closed-form solution without the need for redundant iterative calculations.Our approach,termed generalized CFPM(gCFPM),achieves colorization within seconds for megapixel-scale images,delivering superior colorization quality in terms of both colorfulness and sharpness,along with an extended DOF.Both simulations and experiments demonstrate that gCFPM surpasses state-of-the-art methods across all evaluated criteria.Our work offers a robust and comprehensive workflow for high-throughput full-color pathological imaging using FPM platforms,laying a solid foundation for future advancements in methodology and engineering.
基金supported by the National Key R&D Program(No.2023YFB3709900)the National Nature Science Foundation of China(No.U22A20171)+2 种基金China Baowu Low Carbon Metallurgy Innovation Foundation(No.BWLCF202315)the High Steel Center(HSC)at North China University of TechnologyUniversity of Science and Technology Beijing,China.
文摘The characteristics of nonmetallic inclusions formed during steel production have a significant influence on steel performance.In this paper,studies on inclusions using confocal scanning laser microscopy(CSLM)are reviewed and summarized,particularly the col-lision of various inclusions,dissolution of inclusions in liquid slag,and reactions between inclusions and steel.Solid inclusions exhibited a high collision tendency,whereas pure liquid inclusions exhibited minimal collisions because of the small attraction force induced by their<90°contact angle with molten steel.The collision of complex inclusions in molten steel was not included in the scope of this study and should be evaluated in future studies.Higher CaO/Al_(2)O_(3)and CaO/SiO_(2)ratios in liquid slag promoted the dissolution of Al_(2)O_(3)-based in-clusions.The formation of solid phases in the slag should be prevented to improve dissolution of inclusions.To accurately simulate the dissolution of inclusions in liquid slag,in-situ observation of the dissolution of inclusions at the steel-slag interface is necessary.Using a combination of CSLM and scanning electron microscopy-energy dispersive spectroscopy,the composition and morphological evolution of the inclusions during their modification by the dissolved elements in steel were observed and analyzed.Although the in-situ observa-tion of MnS and TiN precipitations has been widely studied,the in-situ observation of the evolution of oxide inclusions in steel during so-lidification and heating processes has rarely been reported.The effects of temperature,heating and cooling rates,and inclusion character-istics on the formation of acicular ferrites(AFs)have been widely studied.At a cooling rate of 3-5 K/s,the order of AF growth rate in-duced by different inclusions,as reported in literature,is Ti-O<Ti-Ca-Zr-Al-O<Mg-O<Ti-Zr-Al-O<Mn-Ti-Al-O<Ti-Al-O<Zr-Ti-Al-O.Further comprehensive experiments are required to investigate the quantitative relationship between the formation of AFs and inclusions.
基金financial support from the National Natural Science Foundation of China(Grant No.61971201)。
文摘High-resolution transmission electron microscopy(HRTEM)promises rapid atomic-scale dynamic structure imaging.Yet,the precision limitations of aberration parameters and the challenge of eliminating aberrations in Cs-corrected transmission electron microscopy constrain resolution.A machine learning algorithm is developed to determine the aberration parameters with higher precision from small,lattice-periodic crystal images.The proposed algorithm is then validated with simulated HRTEM images of graphene and applied to the experimental images of a molybdenum disulfide(MoS_(2))monolayer with 25 variables(14 aberrations)resolved in wide ranges.Using these measured parameters,the phases of the exit-wave functions are reconstructed for each image in a focal series of MoS_(2)monolayers.The images were acquired due to the unexpected movement of the specimen holder.Four-dimensional data extraction reveals time-varying atomic structures and ripple.In particular,the atomic evolution of the sulfur-vacancy point and line defects,as well as the edge structure near the amorphous,is visualized as the resolution has been improved from about 1.75?to 0.9 A.This method can help salvage important transmission electron microscope images and is beneficial for the images obtained from electron microscopes with average stability.
基金supported by University of Macao,China,Nos.MYRG2022-00054-FHS and MYRG-GRG2023-00038-FHS-UMDF(to ZY)the Macao Science and Technology Development Fund,China,Nos.FDCT0048/2021/AGJ and FDCT0020/2019/AMJ and FDCT 0011/2018/A1(to ZY)Natural Science Foundation of Guangdong Province of China,No.EF017/FHS-YZ/2021/GDSTC(to ZY)。
文摘To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral cortex.However,these methods often require complex systems and the effect of age on cerebral embolism has not been adequately studied,although ischemic stroke is strongly age-related.In this study,we propose an optical-resolution photoacoustic microscopy-based visualized photothrombosis methodology to create and monitor ischemic stroke in mice simultaneously using a 532 nm pulsed laser.We observed the molding process in mice of different ages and presented age-dependent vascular embolism differentiation.Moreover,we integrated optical coherence tomography angiography to investigate age-associated trends in cerebrovascular variability following a stroke.Our imaging data and quantitative analyses underscore the differential cerebrovascular responses to stroke in mice of different ages,thereby highlighting the technique's potential for evaluating cerebrovascular health and unraveling age-related mechanisms involved in ischemic strokes.
基金funded by the National Natural Science Foundation of China(No.62305041)the Natural Science Foundation of Liaoning Province(No.2023-MS-103)。
文摘Stochastic optical reconstruction microscopy(STORM),as a typical technique of single-molecule localization microscopy(SMLM),has overcome the diffraction limit by randomly switching fluorophores between fluorescent and dark states,allowing for the precise localization of isolated emission patterns and the super-resolution reconstruction from millions of localized positions of single fluorophores.A critical factor influencing localization precision is the photo-switching behavior of fluorophores,which is affected by the imaging buffer.The imaging buffer typically comprises oxygen scavengers,photo-switching reagents,and refractive index regulators.Oxygen scavengers help prevent photobleaching,photo-switching reagents assist in facilitating the conversion of fluorophores,and refractive index regulators are used to adjust the refractive index of the solution.The synergistic interaction of these components promotes stable blinking of fluorophores,reduces irreversible photobleaching,and thereby ensures high-quality super-resolution imaging.This review provides a comprehensive overview of the essential compositions and functionalities of imaging buffers used in STORM,serving as a valuable resource for researchers seeking to select appropriate imaging buffers for their experiments.
基金The National Key Research and Development Program of China,Grant/Award Number:2022YFF0710700Nonprofit Central Research Institute Fund of Chinese Academy of Medical Sciences,Grant/Award Number:2023-PT180-01。
文摘Background:Glioblastoma(GBM)is a highly aggressive brain tumor characterized by aberrant angiogenesis and an immunosuppressive microenvironment.Pericytes are aberrantly recruited but their spatiotemporal roles and molecular changes remain unclear.This study investigated platelet-derived growth factor receptor beta-positive(Pdgfrb+)pericyte dynamics and reprogramming in GBM vasculature.Methods:We generated GL261-Luc and GL261-CFP glioblastoma cells via lentiviral transduction and established two transgenic models.(1)For pericyte labeling,Ai14 reporter mice was crossed with PDGFRβ-P2A-CreERT2mice for td Tomato-specific lineage tracing(PT mice).(2)For conditional ablation,we generated inducible Pdgfrb-expressing cell ablation models(PT mice was crossed with ROSA-DTA mice).An intravital imaging platform(FITC-dextran/CFP/td Tomato+two-photon microscopy)tracked pericytes,vessels,and tumor cells,while FACSsorted Pdgfrb+cells from GBM and normal brain were analyzed by LC-MS/MS proteomics.Results:Cre-mediated ablation of Pdgfrb-expressing cells revealed stage-dependent effects on GBM growth:early ablation inhibited progression while late ablation promoted it.Pericytes undergo dual spatial reorganization in GBM:regional enrichment with pre-sprouting accumulation at the tumor-brain interface,and focal positioning with preferential localization at vascular branch points.Concurrently,GBM vasculature displayed simplified branching,dilation,and pericyte remodeling(shorter processes,higher density).Proteomics revealed 1426 altered proteins,with upregulated proliferation pathways(e.g.,matrix metallopeptidase 14[Mmp14],lysyl oxidase like 2[Loxl2])and downregulated homeostasis functions(e.g.,transforming growth factor beta 1[Tgfb1]),validated by scRNA-seq in human GBM.Conclusions:This study demonstrates that during early GBM progression,pericytes actively drive tumor angiogenesis through molecular reprogramming toward proliferative and pro-angiogenic phenotypes,with the integrated imaging-proteomics framework revealing potential therapeutic targets for disrupting pericyte-mediated vascular remodeling.
基金supported by the National Natural Science Foundation of China[Grant Nos.62205367 and 62141506]the Suzhou Basic Research Pilot Project[Grant Nos.SSD2023006 and SJC2021013]the National Key Research and Development Program of China[Grant No.2023YFF1205700].
文摘Maintaining the s-polarization state of laser beams is important to achieve high modulation depth in a laser-interference-based super-resolution structured illumination microscope(SR-SIM).However,the imperfect optical components can depolarize the laser beams hence degenerating the modulation depth.Here,we first presented a direct measurement method designed to estimate the modulation depth more precisely by shifting illumination patterns with equal phase steps.This measurement method greatly reduces the dependence of modulation depths on the samples,and then developed a polarization optimization method to achieve high modulation depth at all orientations by actively and quantitatively compensating for the additional phase difference using a combination of waveplate and a liquid crystal variable retarder(LCVR).Experimental results demonstrate that our method can achieve illumination patterns with modulation depth higher than 0.94 at three orientations with only one LCVR voltage,which enables isotropic resolution improvement.
基金supported by the Samsung Research Funding and Incubation Center of Samsung Electronics(Grant No.SRFC-IT2002-03)the Samsung Electronics Co.,Ltd.(Grant No.IO220908-02403-01)+2 种基金the National Research Foundation of Korea(NRF)grant funded by the Korean government(MSIT)(Grant Nos.NRF-RS-2021-NR060086 and NRF-RS-2023-00251628)the Bio&Medical Technology Development Program of the National Research Foundation funded by the Korean government(MSIT)(Grant No RS-2024-00397673)the KAIST-CERAGEM Next Generation Healthcare Research Center.
文摘Holographic microscopy has emerged as a vital tool in biomedicine,enabling visualization of microscopic morphological features of tissues and cells in a label-free manner.Recently,deep learning(DL)-based image reconstruction models have demonstrated state-of-the-art performance in holographic image reconstruction.However,their utility in practice is still severely limited,as conventional training schemes could not properly handle out-of-distribution data.Here,we leverage backpropagation operation and reparameterization of the forward propagator to enable an adaptable image reconstruction model for histopathologic inspection.Only given with a training dataset of rectum tissue images captured from a single imaging configuration,our scheme consistently shows high reconstruction performance even with the input hologram of diverse tissue types at different pathological states captured under various imaging configurations.Using the proposed adaptation technique,we show that the diagnostic features of cancerous colorectal tissues,such as dirty necrosis,captured with 5×magnification and a numerical aperture(NA)of 0.1,can be reconstructed with high accuracy,whereas a given training dataset is strictly confined to normal rectum tissues acquired under the imaging configuration of 20×magnification and an NA of 0.4.Our results suggest that the DL-based image reconstruction approaches,with sophisticated adaptation techniques,could offer an extensively generalizable solution for inverse mapping problems in imaging.
基金financially supported by the National Natural Science Foundation of China(No.22272039)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB36000000)+1 种基金the Youth Program of the Liaoning Education Department(No.LJKQZ20222280)the Jilin Chinese Academy of Sciences-Yanshen Technology Co.,Ltd.
文摘The assembly behaviors of two low-symmetric carboxylic acid molecules(50-(6-carboxynaphthalen-2-yl)-[1,10:30,100-triphenyl]-3,400,5-tricarboxylic acid(CTTA)and 30,50-bis(6-carboxynaphthalen-2-yl)-[1,10-biphenyl]-3,5-dicarboxylic acid(BCBDA))containing naphthalene rings on graphite surfaces have been investigated using scanning tunneling microscopy(STM).The transformation of nanostructures induced by the second components(EDA and PEBP-C4)have been also examined.Both CTTA and BCBDA molecules self-assemble at the 1-heptanoic acid(HA)/HOPG interface,forming porous network structures.The dimer represents the most elementary building unit due to the formation of double hydrogen bonds.Moreover,the flipping of naphthalene ring results in the isomerization of BCBDA molecule.The introduction of carboxylic acid derivative EDA disrupts the dimer,which subsequently undergoes a structural conformation to form a novel porous structure.Furthermore,upon the addition of pyridine derivative PEBP-C4,N–H⋯O hydrogen bonds are the dominant forces driving the three coassembled structures.We have also conducted density functional theory(DFT)calculations to determine the molecular conformation and analyze the mechanisms underlying the formation of nanostructures.
基金supported by the National Natural Science Foundation of China(52301177)。
文摘The advancement of electron microscopy technology has driven the development of electron microscopes that can apply mechanical loading while observing samples,providing a valuable tool for In-Situ mechanical characterization of materials.In response to the need to characterize the evolution of the mechanical behavior of structural materials,such as aerospace materials,in real cryogenic service environments,and to provide an experimental basis for improving their macroscopic cryogenic mechanical properties,the advancement of In-Situ characterization techniques capable of offering both cryogenic environments and mechanical loading has become imperative.There have been scholars using this technique to carry out cryogenic mechanical In-Situ studies of related materials,with experimental studies dominating in general,and a few reviews of mechanical characterization techniques mentioning cryogenic temperatures.In order to make it easier to conduct research using such characterization techniques and to further promote the development of related characterization techniques,this review compiles the previous work and summarizes the electron microscope-based In-Situ characterization techniques for cryogenic micro-and nanomechanics.These techniques primarily include transmission electron microscopy-based cryogenic tensile and indentation methods,as well as scanning electron microscopy-based cryogenic tensile,indentation,compression,and bending methods.Furthermore,the review outlines the prospective future development of In-Situ characterization techniques for cryogenic micro-and nanomechanics.
基金supported by National Natural Science Foundation of China(62227818,12204239,62275121)Youth Foundation of Jiangsu Province(BK20220946)+1 种基金Fundamental Research Funds for the Central Universities(30923011024)Open Research Fund of Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense(JSGP202201).
文摘Deep learning(DL)is making significant inroads into biomedical imaging as it provides novel and powerful ways of accurately and efficiently improving the image quality of photoacoustic microscopy(PAM).Off-the-shelf DL models,however,do not necessarily obey the fundamental governing laws of PAM physical systems,nor do they generalize well to scenarios on which they have not been trained.In this work,a physics-embedded degeneration learning(PEDL)approach is proposed to enhance the image quality of PAM with a self-attention enhanced U-Net network,which obtains greater physical consistency,improves data efficiency,and higher adaptability.The proposed method is demonstrated on both synthetic and real datasets,including animal experiments in vivo(blood vessels of mouse's ear and brain).And the results show that compared with previous DL methods,the PEDL algorithm exhibits good performance in recovering PAM images qualitatively and quantitatively.It overcomes the challenges related to training data,accuracy,and robustness which a typical data-driven approach encounters,whose exemplary application envisions to provide a new perspective for existing DL tools of enhanced PAM.
基金supported by the German Research Foundation(DFG)(Grant Nos.LE 992/14-3 and LE 992/15-3).
文摘Microsphere and microcylinder-assisted microscopy(MAM)has grown steadily over the last decade and is still an intensively studied optical far-field imaging technique that promises to overcome the fundamental lateral resolution limit of microscopy.However,the physical effects leading to resolution enhancement are still frequently debated.In addition,various configurations of MAM operating in transmission mode as well as reflection mode are examined,and the results are sometimes generalized.We present a rigorous simulation model of MAM and introduce a way to quantify the resolution enhancement.The lateral resolution is compared for microscope arrangements in reflection and transmission modes.Furthermore,we discuss different physical effects with respect to their contribution to resolution enhancement.The results indicate that the effects impacting the resolution in MAM strongly depend on the arrangement of the microscope and the measurement object.As a highlight,we outline that evanescent waves in combination with whispering gallery modes also improve the imaging capabilities,enabling super-resolution under certain circumstances.This result is contrary to the conclusions drawn from previous studies,where phase objects have been analyzed,and thus further emphasizes the complexity of the physical mechanisms underlying MAM.
基金the Association SSF Strategic Mobility Grant(No.SM22-0039)theÅForsk Foundation(No.23-540)for supporting the research regarding inclusion engineering.
文摘High-temperature confocal scanning laser microscopy(HT-CSLM)is a potent methodology for investigating various phenomena in the field of metallurgy.Initially applied to the observation of solid phase transformations and solidification,this method has gained traction in the field of non-metallic inclusion in steels in recent years.An overview of the experimental capabilities of HT-CSLM and the most important results of recent investigations regarding the topics of clean steel production are provided.It includes the formation of intragranular acicular ferrite(IAF)from the surface of non-metallic inclusions during the continuous cooling and heat treatment,which can be especially beneficial in the toughness of heat-affected zones of welded pieces.Furthermore,the investigation of agglomeration mechanisms of non-metallic inclusions(NMIs)in liquid steel is discussed to improve the insight into attraction forces between particles and clogging phenomena during continuous casting.Also,the dissolution of NMIs in various steelmaking slags can be observed by HT-CSLM to compare dissolution rates and mechanisms of NMI,where significant influences of temperature and chemical composition of the slag were shown.Last but not least,the experimental work regarding the interface between steel and slag is discussed,where novel techniques are currently being developed.A comprehensive summary of experimental techniques using HT-CSLM equipment to investigate different interactions of NMIs with steel and slag phases is compiled.
基金funded by the Science and Technology Research Program of Chongqing Municipal Education Commission(KJZD-K202301105,KJQN202201107)the Scienti¯c and Technological Transformative Program of Chongqing Banan District(KY202208161124020).
文摘Traumatic penumbra(TP)is a region with recoverable potential around the primary lesion of brain injury.Rapid and accurate imaging for identifying TP is essential for treating traumatic brain injury(TBI).In this study,we first established traumatic brain injuries(TBIs)in rats using a modified Feeney method,followed by label-free imaging of brain tissue sections with multiphoton fluorescence microscopy.The results showed that the technique effectively imaged normal and traumatic brain tissues,and revealed pathological features such as extracellular matrix changes,vascular cell proliferation,and intracellular edema in the traumatic penumbra.Compared with normal brain tissue,the extracellular matrix in the TP was sparse,cells were disorganized,and hyperplastic vascular cells emitted higher two-photon excited fluorescence(TPEF)signals.Our research demonstrates the potential of multiphoton fluorescence technology in the rapid diagnosis and therapeutic evaluation of TBI.
基金supported by the National Natural Science Foundation of China under Grant No.21933006.
文摘To eliminate distortion caused by vertical drift and illusory slopes in atomic force microscopy(AFM)imaging,a lifting-wavelet-based iterative thresholding correction method is proposed in this paper.This method achieves high-quality AFM imaging via line-by-line corrections for each distorted profile along the fast axis.The key to this line-by-line correction is to accurately simulate the profile distortion of each scanning row.Therefore,a data preprocessing approach is first developed to roughly filter out most of the height data that impairs the accuracy of distortion modeling.This process is implemented through an internal double-screening mechanism.A line-fitting method is adopted to preliminarily screen out the obvious specimens.Lifting wavelet analysis is then carried out to identify the base parts that are mistakenly filtered out as specimens so as to preserve most of the base profiles and provide a good basis for further distortion modeling.Next,an iterative thresholding algorithm is developed to precisely simulate the profile distortion.By utilizing the roughly screened base profile,the optimal threshold,which is used to screen out the pure bases suitable for distortion modeling,is determined through iteration with a specified error rule.On this basis,the profile distortion is accurately modeled through line fitting on the finely screened base data,and the correction is implemented by subtracting the modeling result from the distorted profile.Finally,the effectiveness of the proposed method is verified through experiments and applications.
