AIMTo develop a simplified bioartificial liver (BAL) device prototype, suitable to use freshly and preserved liver Microorgans (LMOs) as biological component. METHODSThe system consists of 140 capillary fibers through...AIMTo develop a simplified bioartificial liver (BAL) device prototype, suitable to use freshly and preserved liver Microorgans (LMOs) as biological component. METHODSThe system consists of 140 capillary fibers through which goat blood is pumped. The evolution of hematocrit, plasma and extra-fiber fluid osmolality was evaluated without any biological component, to characterize the prototype. LMOs were cut and cold stored 48 h in BG35 and ViaSpan<sup>®</sup> solutions. Fresh LMOs were used as controls. After preservation, LMOs were loaded into the BAL and an ammonia overload was added. To assess LMOs viability and functionality, samples were taken to determine lactate dehydrogenase (LDH) release and ammonia detoxification capacity. RESULTSThe concentrations of ammonia and glucose, and the fluids osmolalities were matched after the first hour of perfusion, showing a proper exchange between blood and the biological compartment in the minibioreactor. After 120 min of perfusion, LMOs cold preserved in BG35 and ViaSpan<sup>®</sup> were able to detoxify 52.9% ± 6.5% and 53.6% ± 6.0%, respectively, of the initial ammonia overload. No significant differences were found with Controls (49.3% ± 8.8%, P ®</sup> cold preserved LMOs, respectively (n = 6, P CONCLUSIONThis prototype relied on a simple design and excellent performance. It’s a practical tool to evaluate the detoxification ability of LMOs subjected to different preservation protocols.展开更多
To address the issue of residual pollution caused by polyethylene mulch,this study explored the effects of different mulching methods on the soil environment of the yam field,as well as on yam yield and quality.The ex...To address the issue of residual pollution caused by polyethylene mulch,this study explored the effects of different mulching methods on the soil environment of the yam field,as well as on yam yield and quality.The experiment comprised six treatments in total:one non-mulched treatment served as the control(CK),along with five different film-mulched treatments,namely PE,FZS12,FZS15,FC12,and FC15.The degradation of these films and their effects on soil physicochemical properties,microbial community,yam yield and quality were compared.The results showed that the FZS12 treatment achieved grade 5 degradation by the end of the planting period.Compared with PE treatment,the total soluble sugar content and yield of yam treated with FZS12 were significantly increased by 35.78%and 74.97%,respectively(p<0.05).Compared with CK and PE treatments,FZS12 significantly increased soil available nitrogen by 31.62%and 6.20%,respectively(p<0.05),and significantly increased soil available phosphorus by 8.58%and 4.45%,respectively(p<0.05).Soil pH,available nitrogen,and available phosphorus were the main environmental factors affecting the soil bacterial community.The FZS12 treatment significantly increased the relative abundances of soil bacteria phylum including Acidobacteriota,Myxococcota,Patescibacteria,and Proteobacteria compared with the CK and PE treatments.Functional prediction using Picrust2 revealed that the FZS12 treatment had significantly higher levels of signal transduction and amino acid metabolism than the CK and PE treatments.In conclusion,covering with 12μm PBAT/PLA humic acid biodegradable film enhances yam yield and total soluble sugar content by shaping beneficial soil microbial communities,activating soil nutrients.展开更多
Rice fields are one of the largest sources of methane(CH4),a potent greenhouse gas contributing significantly to global warming.Elucidating the underlying mechanisms and mitigating CH4 emissions from paddy fields is c...Rice fields are one of the largest sources of methane(CH4),a potent greenhouse gas contributing significantly to global warming.Elucidating the underlying mechanisms and mitigating CH4 emissions from paddy fields is crucial for combating climate change while ensuring sustainable food production.This review investigates the biological processes governing CH4 generation in rice fields,focusing on how soil microorganisms generate CH4 under waterlogged,anaerobic conditions.It also explores the mechanisms by which CH4 escapes into the atmosphere through plant-mediated transport,diffusion,and ebullition.Several factors influencing CH4 emissions are discussed,including soil composition,water management,exogenous organic matter application,rice variety selection,and local climate conditions.Strategies that can be implemented to reduce CH4 emissions are assessed,such as alternate wetting and drying,urea deep placement,biochar application,optimized fertilizer application,and breeding of rice varieties with low CH4 emissions.Novel solutions,such as the enhancement of methane-consuming bacteria in soils using microbial-based approaches,are also explored.The importance of integrating innovative technologies,improved farming practices,and interdisciplinary research is emphasized to develop practical and scalable strategies for reducing CH4 emissions.By addressing these challenges,we can advance towards the attainment of sustainable agriculture and global climate goals.This review aims to serve as a comprehensive resource for researchers,policymakers,and practitioners seeking to understand and mitigate CH4 emissions from rice cultivation.展开更多
Environment serves as the pivotal medium to produce fermented food,with fluctuations in environmental factors exerting a profound impact on the modulation of fermentation microbial communities.Such shifts are crucial ...Environment serves as the pivotal medium to produce fermented food,with fluctuations in environmental factors exerting a profound impact on the modulation of fermentation microbial communities.Such shifts are crucial for the distinctiveness of fermented food flavor and the variability in quality.Chinese liquor(Baijiu)is one of the typical representatives of spontaneous fermented food.In this review,the multifaceted relationship between regional environmental attributes and the fermentation dynamics of Baijiu was examined,with a spotlight on the strong-flavor,sauce-flavor,and light-flavor varieties.It reveals the influence of regional environmental factors and brewing environmental factors on microbial function and metabolism,which results in the formation of unique flavor characteristics of Baijiu.The 9 main factors affecting the microecology of Baijiu fermentation were further explored,including environmental sensitivity,microbial interactions,biogeographic patterns,and key abiotic factors such as temperature and humidity.Environmental factor management is crucial for controlling microbial community in fermentation.Intelligent detection of the fermentation system is combined with artificial intelligence to realize the digitalization of Baijiu fermentation,with a view to further studying the environmental mechanism or quantitative control relationship of natural fermentation,improving the environmental stability of natural fermentation,and promoting the mechanization and intelligence of fermentation production.展开更多
Ecological floating bed is an important biological remediation method for water pollution control.During the removal of excess nutrients and pollutants,changes in environmental factors affect the characteristics of mi...Ecological floating bed is an important biological remediation method for water pollution control.During the removal of excess nutrients and pollutants,changes in environmental factors affect the characteristics of microorganisms in aquatic ecosystems.To understand the influences of ecological floating beds on size-fractionated microorganisms,we investigated the community assembly and nitrogen metabolic characteristics of three size-fractionated microorganism groups in the ecological floating bed area,using 18S rDNA,16S rDNA metabarcoding,and metagenomic sequencing techniques.Firstly,we discovered substantial differences between size-fractionated groups in the diversity and compositions of both microeukaryotic and bacterial communities,as well as the influences of floating beds on specific groups.The floating beds appeared to provide more habitats for heterotrophs and symbiotes while potentially inhibiting the growth of certain phytoplankton(cyanobacteria).Secondly,we observed that microeukaryotic and bacterial communities were predominantly influenced by stochastic and deterministic processes,respectively,and they both exhibited distinct patterns across different size-fractionated groups.Notably,microeukaryotic community assembly demonstrated a greater sensitivity to ecological floating beds,as indicated by an increase in dispersal limitation processes.Finally,the nitrogen metabolism functional genes revealed that microbes associated with large-sized particles played a crucial role in dissimilatory nitrate reduction to ammonium(DNRA)and denitrification processes within the floating bed area,thereby facilitating the removal of excess nitrogen nutrients from the water.In contrast,freeliving microorganisms from small-sized groups were linked mainly to the genes involved in nitrogen assimilation and assimilatory nitrate reduction to ammonium(ANRA)processes.These findings help understand the impact of ecological floating beds on the diversity and functional characteristics of microorganism communities in different size-fractionated groups.展开更多
We mixed Bacillus subtilis and brewing yeast to prepare composite microbial self-healing materials,and studied the self-healing effect of composite microorganisms in mortar cracks of different widths and cracking ages...We mixed Bacillus subtilis and brewing yeast to prepare composite microbial self-healing materials,and studied the self-healing effect of composite microorganisms in mortar cracks of different widths and cracking ages.The experimental results show that the performance and self-healing effect of composite micro-organisms are significantly better than those of single microorganisms.For cracks with widths of 0.2-0.4 mm,the repair effect of the composite microorganisms at 28 days is 42.7%and 71.2%higher than that of pure Bacillus and pure yeast,respectively.The repairing rate of the area with the widths of the cracks of 0.2-0.4,0.4-0.6,and 0.6-0.8 mm are 100%,77.3%,and 53.4%,respectively.The area repair rates corresponding to cracking ages of 56,90,and 180 days are 73.3%,55.4%,and 30.8%,respectively.展开更多
Cholangiocarcinoma(CCA)is a highly malignant tumor of the biliary tract with a poor prognosis.Currently,specific methods for the early diagnosis and risk stratification if CCA are lacking.With the emergence of the“gu...Cholangiocarcinoma(CCA)is a highly malignant tumor of the biliary tract with a poor prognosis.Currently,specific methods for the early diagnosis and risk stratification if CCA are lacking.With the emergence of the“gut-biliary-liver axis”concept,the intestinal and biliary microbiota are being increasingly recognized to play key roles in the initiation and progression of CCA.This review systematically synthesizes recent clinical and basic research and outlines characteristic patterns of dysbiosis in the feces,bile,and tumor tissues of patients with CCA.It further discusses key mechanisms,including microbiota-bile acid-biliary epithelial signaling,pathogen-associated molecular patterns-mediated chronic inflammation,and immune-metabolic remodeling.It also examines the associations of these mechanisms with tumor progression and treatment responses.On this basis,the review evaluates the potential of intestinal and biliary microbiota and their metabolites as biomarkers for the diagnosis,prognosis,and prediction of the treatment response of CCA.We believe this review demonstrates a theoretical basis for microbiota-targeted precision prevention and therapeutic strategies for the disease.展开更多
Microbial degradation is the primary mechanism for purifying polycyclic aromatic hydrocarbon(PAH)contamination in environments,and biochar immobilization is an effective technology to enhance biodegradation,but the pr...Microbial degradation is the primary mechanism for purifying polycyclic aromatic hydrocarbon(PAH)contamination in environments,and biochar immobilization is an effective technology to enhance biodegradation,but the process parameters of the immobilization technology still require further systematic evaluation.Here,biochars derived from pig manure(PM),bamboo(BB),rice straw(RS),and soybean straw(SS)were used as carriers of Mycobacterium sp.ZL7,and the optimal biochar dosage of 1:30(w/V)and immobilization time of 24 h were determined.The immobilization effects followed the order of RS>SS>PM>BB.Scanning electron microscopy and physicochemical properties revealed that porous structures acted as shelters for bacteria,and high nitrogen content,large pore size and high-water holding capacity played important driving roles in immobilization.In the single-substrate system,pyrene removal rates of the PM-,RS-and SS-immobilized materials were greater than 96%,which were significantly higher than those of the biochar alone or the free strain.An orthogonal design experiment in historically PAH-contaminated soil further revealed that,compared with free strains,immobilized materials combined with high moisture content and moderate salicylic acid or Brij 30 can effectively increase the abundance of bacteria and the nidA gene,and enhance the dehydrogenase and polyphenol oxidase activities.The removal rate of total PAHs increased by 8.53%-30.45%after 24 d.Moreover,biochar with strong immobilization capacity showed better PAH removal effects.This study provides a scientific basis and practical reference for biochar-immobilized microorganisms to enhance the self-purification of PAH-contaminated soil.展开更多
To enhance the stabilization performance of red-bedded mudstone subgrade soil,a novel synergistic technique combining microorganisms and quicklime was developed and evaluated through comprehensive physical,mechanical,...To enhance the stabilization performance of red-bedded mudstone subgrade soil,a novel synergistic technique combining microorganisms and quicklime was developed and evaluated through comprehensive physical,mechanical,and microstructural analyses.The results demonstrated that quicklime significantly improved the pore structure by increasing the proportion of pores with diameters in the range of 0.5-50μm,thereby facilitating microbial migration and calcium carbonate precipitation.The proposed technique effectively enhanced cementitious material production(cementation rate:6.83%),unconfined compressive strength(1.63 MPa),and resistance to swelling and deformation(swelling rate:0.26%).The observed synergistic effect can be attributed to two primary mechanisms.First,quicklime promotes soil particle agglomeration,resulting in larger pores that enable bacterial mobility and provide optimal microenvironments for microbial activity.Second,during curing,quicklime undergoes hydration and ionization,releasing Ca^(2+)and OH^(−)ions.The Ca^(2+)ions serve as calcium sources for microbial-induced carbonate precipitation,while the OH−ions react with SiO_(2)and Al_(2)O_(3) to form hydrated gels that develop a skeletal soil structure.Meanwhile,the exothermic reaction further accelerates calcium carbonate deposition within the agglomerated pores.展开更多
With the changing climate and escalating population,there will be extreme pressure on agricultural food production to ensure global food security.Traditional agricultural practices have relied heavily on hazardous pes...With the changing climate and escalating population,there will be extreme pressure on agricultural food production to ensure global food security.Traditional agricultural practices have relied heavily on hazardous pesticides and chemical fertilizers to boost crop yields.However,their continuous and excessive use has caused significant harm to non-target organisms,including humans,while also leading to a severe decline in soil health due to their indiscriminate and unbalanced application.Hence,serious efforts are needed to control this mounting problem of soil and environmental pollution.One effective strategy involves using microorganisms capable of solubilizing nutrients and breaking down pesticides.These microorganisms improve crop nutrient absorption by solubilizing essential nutrients and simultaneously degrade pesticide residues in soil.Utilizing this ability of microorganisms to degrade agrochemicals,microbial remediation offers a dependable and economical method for reducing the effects of such unwarranted contaminants.This review presents an extensive overview of pesticide use as well as microorganisms in soil as pesticide degraders,nutrient mobilizers(phosphate(PO_(4)^(3-)-P),potassium(K),and zinc(Zn)),and plant growth promoters for preventing the unsustainable exploitation of natural reserves.This review aims to highlight the diverse benefits these microorganisms offer across various domains while presenting an exciting opportunity to advance sustainable agriculture and firstly establishes a connection between nutrient solubilization and pesticide degradation mediated by microorganisms.It also offers a comprehensive bibliographic review of the application of plant growth-promoting microorganisms for solubilizing nutrients,such as P,K,and Zn,and degrading pesticides as well.展开更多
Wetlands are vital ecosystems that perform essential functions such as climate regulation,environmental purification,material circulation and energy flow.They play an essential role in global biogeochemical cycles,dri...Wetlands are vital ecosystems that perform essential functions such as climate regulation,environmental purification,material circulation and energy flow.They play an essential role in global biogeochemical cycles,driven primarily by microorganisms.Understanding the distribution of wetland microorganisms across different temperature zones is key to comprehending their ecological roles.A meta-analysis of 704 wetland soil samples on microbial communities was conducted,using statistical methods like analysis of variance,principal component analysis,non-metric nultidimensional scaling,and structural equation modeling to examine biogeography and diversity across temperature zones.Our findings revealed a clear latitudinal diversity gradient pattern,with the highest microbial abundance in the tropics and the lowest in the southern temperate zone,which differed significantly from other temperature zones.Proteobacteria(37.76%-51.04%),Acidobacteria(5.11%-30.70%)and Bacteroidetes(3.43%-16.16%)dominanted the bacterial communities.Notably,the southern temperate zone showed significant variations,with a higher prevalence of Acidobacteria(30.07%).To investigate the causes of this variability,we screened 177 core microbiome and identified latitude as the core environmental factor influencing microbial community composition.Moreover,soil microorganisms exhibited strong nitrogen cycling potentials(particularly nirD and nirB)and carbon cycling potentials(especially accA),with gene abundances showing little variation across temperature zones.Wetland bacterial communities also demonstrated high stability,with average variation degree index values ranging from 0.1 to 0.3.Our results improve the understanding of the diversity and biogeographic mechanisms of wetland bacterial communities and hold significant implications for the management and conservation of wetlands.展开更多
Objective To examine national trends in antibiotic consumption and antimicrobial resistance(AMR)among six WHO-priority bacterial pathogens in China from 2016 to 2022.Methods This ecological study analyzed national and...Objective To examine national trends in antibiotic consumption and antimicrobial resistance(AMR)among six WHO-priority bacterial pathogens in China from 2016 to 2022.Methods This ecological study analyzed national and provincial data from the China Antibacterial Resistance Surveillance System(CARSS)and the National Hospital Information Network.Beta regression models assessed temporal trends,and hierarchical models evaluated associations between antibiotic use and resistance.Results From 2016 to 2022,carbapenem resistance in Acinetobacter baumannii and Pseudomonas aeruginosa,and vancomycin resistance in Enterococcus faecium and E.faecalis significantly declined(β<0,P<0.010),while carbapenem-resistant Klebsiella pneumoniae increased(β=0.081,P<0.001).Nationwide antibiotic consumption rose across 10 major classes.Positive associations were found between carbapenem use and resistance in A.baumannii(z=2.719,P=0.007)and P.aeruginosa(z=3.241,P=0.001),and between vancomycin use and resistance in E.faecium(z=4.510,P=0.001)and E.faecalis(z=3.210,P=0.001).Conclusion Carbapenem-resistant K.pneumoniae increased significantly in China,while other resistant pathogens declined.Resistance patterns were linked to the use of multiple antibiotic classes,underscoring the need for strengthened antibiotic stewardship and surveillance.展开更多
Rhizobial inoculation in soybean is an effective strategy in sustainable agriculture to reduce chemical fertilizer application and to increase crop production.It not only provides nitrogen sources for host plants but ...Rhizobial inoculation in soybean is an effective strategy in sustainable agriculture to reduce chemical fertilizer application and to increase crop production.It not only provides nitrogen sources for host plants but also improves the rhizosphere soil environment.However,the inoculation efficiency of rhizobia remains to be improved.In this study,we investigated the nodulation efficiency of Bradyrhizobium and Sinorhizobium strains under different soil conditions and evaluated their impacts on the rhizocompartment bacterial community.We found that inoculation with Bradyrhizobium diazoefficiens UASD 110 increased the number of soybean nodules in acidic soil,while Sinorhizobium fredii CCBAU 45436 was more effective in alkaline soil.However,inoculation with neither strain significantly affected nodulation in neutral soil.Then,we demonstrated that UASD 110 was more competitive in nodulation than CCBAU 45436,which was related to its higher abundance in the rhizosphere.Furthermore,we showed that while single inoculation with UASD 110 or CCBAU 45436 failed to alter the bacterial diversity,these two strains differentially influenced the rhizosphere microbial composition.Finally,we identified the main rhizosphere microorganisms that were affected by these two strains.Our findings revealed that the nodulation capacity of rhizobia and their colonization of rhizosphere and nodules are soil-type dependent,yet their impact on the rhizobacterial community exhibited consistent patterns.These findings provide valuable insights into optimizing rhizobial inoculation strategies to enhance nitrogen fixation efficiency.展开更多
Formate bioconversion plays a crucial role in achieving renewable resource utilization and green and sustainable development,as it helps convert formate to biofuels and biochemicals.However,to tap the full potential o...Formate bioconversion plays a crucial role in achieving renewable resource utilization and green and sustainable development,as it helps convert formate to biofuels and biochemicals.However,to tap the full potential of formate bioconversion,it is important to identify the most appropriate microbial hosts,design the most promising formate assimilation pathways,and develop the most efficient formate assimilation cell factories.Here,we summarize the formatotrophic microorganisms capable of assimilating formate into building blocks of cell growth and analyze the characteristics of formate assimilation pathways for transmitting formate into central carbon metabolism.Furthermore,we discuss microbial engineering strategies to improve the efficiency of formate utilization for producing high-value bioproducts.Finally,we highlight the key challenges of formate bioconversion and their possible solutions to advance the formate bioeconomy and biomanufacturing.展开更多
Small RNAs(sRNAs)are important non-coding RNAs that usually play crucial roles in gene expression at the post-transcriptional level.The sRNAs have mostly been investigated in model microorganisms such as Escherichia c...Small RNAs(sRNAs)are important non-coding RNAs that usually play crucial roles in gene expression at the post-transcriptional level.The sRNAs have mostly been investigated in model microorganisms such as Escherichia coli and some pathogens.Nevertheless,microbial sRNAs from extreme environments such as the polar regions and deep sea have recently been discovered and analyzed for their unique roles in stress response,metabolic regulation and adaptation to extreme environments.These sRNAs fine-tune gene expression during oxidative and radiation stress,and modulate temperature and osmotic pressure responses.Representative sRNAs and their functions in thermophilic,psychrophilic,halophilic and radiation-tolerant bacteria are summarized in this review.Despite challenges in sample collection,RNA isolation,and functional annotation,the study of sRNAs in extreme environments provides opportunities for discovering novel regulatory mechanisms,applying them to biotechnology,and advancing our understanding of evolutionary adaptations.Looking ahead,high-throughput sequencing,synthetic biology,and multi-omics integration will bring new breakthroughs in discovering novel sRNAs and their functions and regulatory mechanisms.Such advancements are poised to enable comprehensive characterization of sRNA-mediated regulatory networks in extremophiles and unlock their biotechnological potential through mechanism-driven applications.展开更多
The excessive reliance on chemical inputs for managing soil nutrients and pathogens has raised concerns about their long-term sustainability and environmental impact.In contrast,the use of soil microbes offers an eco-...The excessive reliance on chemical inputs for managing soil nutrients and pathogens has raised concerns about their long-term sustainability and environmental impact.In contrast,the use of soil microbes offers an eco-friendly and efficient alternative for improving soil fertility and plant growth.Beneficial microorganisms,including plant growth-promoting rhizobacteria(PGPR),mycorrhizal fungi,and other soil organisms,play pivotal roles in nutrient cycling,organic matter decomposition,and nutrient availability improvement.This review explores the potential of leveraging microbial resources for sustainable soil nutrient management and resilient crop production.It delves into the intricate interactions between host plants and PGPR,particularly under nutrient-limited and fluctuating environmental conditions,with a focus on the molecular signaling pathways and mechanisms regulating these relationships.Furthermore,it emphasizes the role of advanced techniques and PGPR-responsive microRNAs to uncover the functional capabilities of microbial communities and their dynamic interactions with plants.These approaches pave the way for developing innovative,microbe-based strategies to optimize nutrient use efficiency,reduce dependency on synthetic fertilizers,and support sustainable agricultural practices.展开更多
Early prevention and control of coal spontaneous combustion have emerged as a critical research area in coal mine safety.Due to their sustainability and environmental friendliness,microorganisms have gained attention....Early prevention and control of coal spontaneous combustion have emerged as a critical research area in coal mine safety.Due to their sustainability and environmental friendliness,microorganisms have gained attention.A filamentous fungus was collected in the coal mine and identified as Absidia spinosa.Results indicated that the mycelium effectively covered and repaired many coal pores.The oxygen consumption ratio of A.spinosa was higher in coal-containing environments than in coal-free conditions.The fungus significantly impacted aliphatic functional groups,disrupting bridging bonds and side chains connected to aromatic structures and reducing the relative content of C—O bonds.Additionally,A.spinosa increases the ignition temperature by 25.34℃.The total heat release was decreased by approximately 32.58%,and the activation energies were increased.The genome of Absidia spinosa revealed genes related to oxygen consumption,small molecule degradation,and secretion of metabolic products,such as those annotated under GO ID:0140657,etc.The pathways involved in the degradation of small organic molecules(e.g.,ko00626,etc.),carbon fixation,and nitrogen cycling,all linked to coal decomposition.Through oxygen consumption and the alteration of coal-active structures,A.spinosa effectively inhibits CSC,providing an experimental basis for exploring eco-friendly biological control methods in the goaf.展开更多
Sporosarcina pasteurii was employed as the strain,with an in-situ magnetization construction,to obtain magnetic microorganisms and oriented self-healing mortar specimens based on them.The magnetic field was used to ac...Sporosarcina pasteurii was employed as the strain,with an in-situ magnetization construction,to obtain magnetic microorganisms and oriented self-healing mortar specimens based on them.The magnetic field was used to achieve the directional migration of magnetic microorganisms during the oriented selfhealing of mortar cracks,improving the rate of self-healing of cracks.The experimental results demonstrate that the magnetic microorganisms are composed of Fe_(3)O_(4)nanosheets attached to the surface of Sporosarcina pasteurii,whose mineralization products are comprised of vaterite primarily.Compared with the pure microbial group,the magnetic microbial group exhibits a faster repair rate,shortening the repair time required to achieve an area repair efficiency of over 90%from 28 days to 14 days,thereby doubling the repair rate.Meanwhile,the area repair efficiency of the magnetic microbial group at 7,14,and 28 days are increased by 50.3%,11.2%,and 4.6%,respectively,compared to the pure microbial group,which are due to the magnetic microorganisms'superior directional migration and mineralization ability,exceeding that of the ordinary microorganisms.展开更多
The present study investigates the flow,heat,and mass transfer analysis in the bioconvection of nanofluid containing motile gyrotactic microorganisms through a semi-porous curved oscillatory channel with a magnetic fi...The present study investigates the flow,heat,and mass transfer analysis in the bioconvection of nanofluid containing motile gyrotactic microorganisms through a semi-porous curved oscillatory channel with a magnetic field.These microorganisms produce density gradients by swimming,which induces macroscopic convection flows in the fluid.This procedure improves the mass and heat transfer,illustrating the interaction between biological activity and fluid dynamics.Furthermore,instead of considering traditional Fourier's and Fick's law the energy and concentration equations are developed by incorporating Cattaneo-Christov double diffusion theory.Moreover,to examine the influence of thermophoresis and Brownian diffusions in the fluid we have adopted the Buongiorno nanofluid model.Due to the oscillation of the surface of the channel,the mathematical development of the considered flow problem is obtained in the form of partial differential equations via the curvilinear coordinate system.The convergent series solution of the governing flow equations is obtained after applying the homotopy analysis method(HAM).The effects of different pertinent flow parameters on velocity,motile microorganism density distribution,concentration,pressure,temperature,and skin friction coefficient are examined and discussed in detail with the help of graphs and tables.It is observed during the current study that the density of microorganisms is enhanced for higher values of Reynolds number,Peclet number,radius of curvature variable,and Lewis number.展开更多
Soil microorganisms and labile soil organic carbon(SOC)fractions are essential factors affecting greenhouse gas(GHG)emissions in paddy fields.However,the effects of labile SOC fractions and microorganisms on GHG emiss...Soil microorganisms and labile soil organic carbon(SOC)fractions are essential factors affecting greenhouse gas(GHG)emissions in paddy fields.However,the effects of labile SOC fractions and microorganisms on GHG emissions from flooding to drying after organic fertilizer replacing for chemical fertilizer remain unclear.Here,a long-term experiment was conducted with four treatments:chemical fertilization only(control),organic fertilizer substituting 25%of chemical N fertilizer(NM1),50%of chemical N fertilizer(NM2),and NM2combined with crop straw(NMS).GHG emissions were monitored,and soil samples were collected to determine labile SOC fractions and microorganisms.Results revealed the GHG emissions in the NM2 significantly increased by 196.88%from flooding to drying,mainly due to the higher CO_(2) emissions.The GHG emissions per kg of C input in NMS was the lowest with the value of 9.17.From flooding to drying,organic fertilizer application significantly increased the readily oxidizable organic carbon(ROC)contents and C lability;the NM2 and NMS dramatically increased the SOC and non-readily oxidizable organic carbon(NROC).The bacterial communities showed significant differences among different treatments in the flooding,while the significant difference was only found between the NMS and other treatments in the drying.From flooding to drying,changing soil moisture conditions causes C fractions and microbial communities to jointly affect carbon emissions,and the NMS promoted carbon sequestration and mitigated GHG emissions.Our findings highlight the importance of the labile SOC fractions and microorganisms linked to GHG emissions in paddy fields.展开更多
文摘AIMTo develop a simplified bioartificial liver (BAL) device prototype, suitable to use freshly and preserved liver Microorgans (LMOs) as biological component. METHODSThe system consists of 140 capillary fibers through which goat blood is pumped. The evolution of hematocrit, plasma and extra-fiber fluid osmolality was evaluated without any biological component, to characterize the prototype. LMOs were cut and cold stored 48 h in BG35 and ViaSpan<sup>®</sup> solutions. Fresh LMOs were used as controls. After preservation, LMOs were loaded into the BAL and an ammonia overload was added. To assess LMOs viability and functionality, samples were taken to determine lactate dehydrogenase (LDH) release and ammonia detoxification capacity. RESULTSThe concentrations of ammonia and glucose, and the fluids osmolalities were matched after the first hour of perfusion, showing a proper exchange between blood and the biological compartment in the minibioreactor. After 120 min of perfusion, LMOs cold preserved in BG35 and ViaSpan<sup>®</sup> were able to detoxify 52.9% ± 6.5% and 53.6% ± 6.0%, respectively, of the initial ammonia overload. No significant differences were found with Controls (49.3% ± 8.8%, P ®</sup> cold preserved LMOs, respectively (n = 6, P CONCLUSIONThis prototype relied on a simple design and excellent performance. It’s a practical tool to evaluate the detoxification ability of LMOs subjected to different preservation protocols.
基金supported by the Wencheng County Science and Technology Plan Project(2023NKY03)Earmarked Fund for Modern Agro-industry Technology Research System(Grant Number CARS-24-B04,CARS-23-B05)Additional support was provided by Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Vegetables),Ministry of Agriculture and Rural Affairs,China.
文摘To address the issue of residual pollution caused by polyethylene mulch,this study explored the effects of different mulching methods on the soil environment of the yam field,as well as on yam yield and quality.The experiment comprised six treatments in total:one non-mulched treatment served as the control(CK),along with five different film-mulched treatments,namely PE,FZS12,FZS15,FC12,and FC15.The degradation of these films and their effects on soil physicochemical properties,microbial community,yam yield and quality were compared.The results showed that the FZS12 treatment achieved grade 5 degradation by the end of the planting period.Compared with PE treatment,the total soluble sugar content and yield of yam treated with FZS12 were significantly increased by 35.78%and 74.97%,respectively(p<0.05).Compared with CK and PE treatments,FZS12 significantly increased soil available nitrogen by 31.62%and 6.20%,respectively(p<0.05),and significantly increased soil available phosphorus by 8.58%and 4.45%,respectively(p<0.05).Soil pH,available nitrogen,and available phosphorus were the main environmental factors affecting the soil bacterial community.The FZS12 treatment significantly increased the relative abundances of soil bacteria phylum including Acidobacteriota,Myxococcota,Patescibacteria,and Proteobacteria compared with the CK and PE treatments.Functional prediction using Picrust2 revealed that the FZS12 treatment had significantly higher levels of signal transduction and amino acid metabolism than the CK and PE treatments.In conclusion,covering with 12μm PBAT/PLA humic acid biodegradable film enhances yam yield and total soluble sugar content by shaping beneficial soil microbial communities,activating soil nutrients.
基金supported by the Cooperative Research Program for Agriculture Science and Technology Development,Republic of Korea(Grant No.RS-2022-RD010034)the 2023 Yellow Sea Wetland International Cooperation Key Project,China(Grant No.HHSDKT202303).
文摘Rice fields are one of the largest sources of methane(CH4),a potent greenhouse gas contributing significantly to global warming.Elucidating the underlying mechanisms and mitigating CH4 emissions from paddy fields is crucial for combating climate change while ensuring sustainable food production.This review investigates the biological processes governing CH4 generation in rice fields,focusing on how soil microorganisms generate CH4 under waterlogged,anaerobic conditions.It also explores the mechanisms by which CH4 escapes into the atmosphere through plant-mediated transport,diffusion,and ebullition.Several factors influencing CH4 emissions are discussed,including soil composition,water management,exogenous organic matter application,rice variety selection,and local climate conditions.Strategies that can be implemented to reduce CH4 emissions are assessed,such as alternate wetting and drying,urea deep placement,biochar application,optimized fertilizer application,and breeding of rice varieties with low CH4 emissions.Novel solutions,such as the enhancement of methane-consuming bacteria in soils using microbial-based approaches,are also explored.The importance of integrating innovative technologies,improved farming practices,and interdisciplinary research is emphasized to develop practical and scalable strategies for reducing CH4 emissions.By addressing these challenges,we can advance towards the attainment of sustainable agriculture and global climate goals.This review aims to serve as a comprehensive resource for researchers,policymakers,and practitioners seeking to understand and mitigate CH4 emissions from rice cultivation.
基金financially supported by the National Natural Science Foundation of China(22138004)National Treasure Ecological Research Synergetic Innovation Center.
文摘Environment serves as the pivotal medium to produce fermented food,with fluctuations in environmental factors exerting a profound impact on the modulation of fermentation microbial communities.Such shifts are crucial for the distinctiveness of fermented food flavor and the variability in quality.Chinese liquor(Baijiu)is one of the typical representatives of spontaneous fermented food.In this review,the multifaceted relationship between regional environmental attributes and the fermentation dynamics of Baijiu was examined,with a spotlight on the strong-flavor,sauce-flavor,and light-flavor varieties.It reveals the influence of regional environmental factors and brewing environmental factors on microbial function and metabolism,which results in the formation of unique flavor characteristics of Baijiu.The 9 main factors affecting the microecology of Baijiu fermentation were further explored,including environmental sensitivity,microbial interactions,biogeographic patterns,and key abiotic factors such as temperature and humidity.Environmental factor management is crucial for controlling microbial community in fermentation.Intelligent detection of the fermentation system is combined with artificial intelligence to realize the digitalization of Baijiu fermentation,with a view to further studying the environmental mechanism or quantitative control relationship of natural fermentation,improving the environmental stability of natural fermentation,and promoting the mechanization and intelligence of fermentation production.
基金Supported by the National Natural Science Foundation of China(Nos.42141003,42176147)the National Key Research and Development Program of China(No.2022YFF0802204)the Xiamen Key Laboratory of Urban Sea Ecological Conservation and Restoration(USER)(Nos.USER2021-1,USER2021-5)。
文摘Ecological floating bed is an important biological remediation method for water pollution control.During the removal of excess nutrients and pollutants,changes in environmental factors affect the characteristics of microorganisms in aquatic ecosystems.To understand the influences of ecological floating beds on size-fractionated microorganisms,we investigated the community assembly and nitrogen metabolic characteristics of three size-fractionated microorganism groups in the ecological floating bed area,using 18S rDNA,16S rDNA metabarcoding,and metagenomic sequencing techniques.Firstly,we discovered substantial differences between size-fractionated groups in the diversity and compositions of both microeukaryotic and bacterial communities,as well as the influences of floating beds on specific groups.The floating beds appeared to provide more habitats for heterotrophs and symbiotes while potentially inhibiting the growth of certain phytoplankton(cyanobacteria).Secondly,we observed that microeukaryotic and bacterial communities were predominantly influenced by stochastic and deterministic processes,respectively,and they both exhibited distinct patterns across different size-fractionated groups.Notably,microeukaryotic community assembly demonstrated a greater sensitivity to ecological floating beds,as indicated by an increase in dispersal limitation processes.Finally,the nitrogen metabolism functional genes revealed that microbes associated with large-sized particles played a crucial role in dissimilatory nitrate reduction to ammonium(DNRA)and denitrification processes within the floating bed area,thereby facilitating the removal of excess nitrogen nutrients from the water.In contrast,freeliving microorganisms from small-sized groups were linked mainly to the genes involved in nitrogen assimilation and assimilatory nitrate reduction to ammonium(ANRA)processes.These findings help understand the impact of ecological floating beds on the diversity and functional characteristics of microorganism communities in different size-fractionated groups.
基金Funded by the National Key R&D Program of China(No.2023YFC3806100)the National Nature Science Foundation of China(No.52278269,52278268)+2 种基金the Tianjin Outstanding Young Scholars Science Fund Project(No.22JCJQJC00020)the Key Project of Tianjin Natural Science Foundation(No.23JCZDJC00430)the Joint Research Center of China and Foreign Countries Special Fund of Tianjin Innovation Platform(No.24PTLYHZ00240)。
文摘We mixed Bacillus subtilis and brewing yeast to prepare composite microbial self-healing materials,and studied the self-healing effect of composite microorganisms in mortar cracks of different widths and cracking ages.The experimental results show that the performance and self-healing effect of composite micro-organisms are significantly better than those of single microorganisms.For cracks with widths of 0.2-0.4 mm,the repair effect of the composite microorganisms at 28 days is 42.7%and 71.2%higher than that of pure Bacillus and pure yeast,respectively.The repairing rate of the area with the widths of the cracks of 0.2-0.4,0.4-0.6,and 0.6-0.8 mm are 100%,77.3%,and 53.4%,respectively.The area repair rates corresponding to cracking ages of 56,90,and 180 days are 73.3%,55.4%,and 30.8%,respectively.
文摘Cholangiocarcinoma(CCA)is a highly malignant tumor of the biliary tract with a poor prognosis.Currently,specific methods for the early diagnosis and risk stratification if CCA are lacking.With the emergence of the“gut-biliary-liver axis”concept,the intestinal and biliary microbiota are being increasingly recognized to play key roles in the initiation and progression of CCA.This review systematically synthesizes recent clinical and basic research and outlines characteristic patterns of dysbiosis in the feces,bile,and tumor tissues of patients with CCA.It further discusses key mechanisms,including microbiota-bile acid-biliary epithelial signaling,pathogen-associated molecular patterns-mediated chronic inflammation,and immune-metabolic remodeling.It also examines the associations of these mechanisms with tumor progression and treatment responses.On this basis,the review evaluates the potential of intestinal and biliary microbiota and their metabolites as biomarkers for the diagnosis,prognosis,and prediction of the treatment response of CCA.We believe this review demonstrates a theoretical basis for microbiota-targeted precision prevention and therapeutic strategies for the disease.
基金supported by the National Key Research and Development Project of China(Nos.2023YFC3709700 and 2024YFC3713800).
文摘Microbial degradation is the primary mechanism for purifying polycyclic aromatic hydrocarbon(PAH)contamination in environments,and biochar immobilization is an effective technology to enhance biodegradation,but the process parameters of the immobilization technology still require further systematic evaluation.Here,biochars derived from pig manure(PM),bamboo(BB),rice straw(RS),and soybean straw(SS)were used as carriers of Mycobacterium sp.ZL7,and the optimal biochar dosage of 1:30(w/V)and immobilization time of 24 h were determined.The immobilization effects followed the order of RS>SS>PM>BB.Scanning electron microscopy and physicochemical properties revealed that porous structures acted as shelters for bacteria,and high nitrogen content,large pore size and high-water holding capacity played important driving roles in immobilization.In the single-substrate system,pyrene removal rates of the PM-,RS-and SS-immobilized materials were greater than 96%,which were significantly higher than those of the biochar alone or the free strain.An orthogonal design experiment in historically PAH-contaminated soil further revealed that,compared with free strains,immobilized materials combined with high moisture content and moderate salicylic acid or Brij 30 can effectively increase the abundance of bacteria and the nidA gene,and enhance the dehydrogenase and polyphenol oxidase activities.The removal rate of total PAHs increased by 8.53%-30.45%after 24 d.Moreover,biochar with strong immobilization capacity showed better PAH removal effects.This study provides a scientific basis and practical reference for biochar-immobilized microorganisms to enhance the self-purification of PAH-contaminated soil.
基金supported by the National Natural Science Foundation of China(Grant No.U22A20600)by the Natural Science Foundation of Hubei Province(Grant No.2025AFA015).
文摘To enhance the stabilization performance of red-bedded mudstone subgrade soil,a novel synergistic technique combining microorganisms and quicklime was developed and evaluated through comprehensive physical,mechanical,and microstructural analyses.The results demonstrated that quicklime significantly improved the pore structure by increasing the proportion of pores with diameters in the range of 0.5-50μm,thereby facilitating microbial migration and calcium carbonate precipitation.The proposed technique effectively enhanced cementitious material production(cementation rate:6.83%),unconfined compressive strength(1.63 MPa),and resistance to swelling and deformation(swelling rate:0.26%).The observed synergistic effect can be attributed to two primary mechanisms.First,quicklime promotes soil particle agglomeration,resulting in larger pores that enable bacterial mobility and provide optimal microenvironments for microbial activity.Second,during curing,quicklime undergoes hydration and ionization,releasing Ca^(2+)and OH^(−)ions.The Ca^(2+)ions serve as calcium sources for microbial-induced carbonate precipitation,while the OH−ions react with SiO_(2)and Al_(2)O_(3) to form hydrated gels that develop a skeletal soil structure.Meanwhile,the exothermic reaction further accelerates calcium carbonate deposition within the agglomerated pores.
基金the University Grant Commission(UGC),New Delhi,India,for supporting this work by providing a fellowship under the scheme of National Eligibility Test for Junior Research Fellowship(NET-JRF),India(No.210510284434)Financial support from the Core Research Grant by Science and Engineering Research Board,India(No.SERB-CRG/2022/002534)is appreciated。
文摘With the changing climate and escalating population,there will be extreme pressure on agricultural food production to ensure global food security.Traditional agricultural practices have relied heavily on hazardous pesticides and chemical fertilizers to boost crop yields.However,their continuous and excessive use has caused significant harm to non-target organisms,including humans,while also leading to a severe decline in soil health due to their indiscriminate and unbalanced application.Hence,serious efforts are needed to control this mounting problem of soil and environmental pollution.One effective strategy involves using microorganisms capable of solubilizing nutrients and breaking down pesticides.These microorganisms improve crop nutrient absorption by solubilizing essential nutrients and simultaneously degrade pesticide residues in soil.Utilizing this ability of microorganisms to degrade agrochemicals,microbial remediation offers a dependable and economical method for reducing the effects of such unwarranted contaminants.This review presents an extensive overview of pesticide use as well as microorganisms in soil as pesticide degraders,nutrient mobilizers(phosphate(PO_(4)^(3-)-P),potassium(K),and zinc(Zn)),and plant growth promoters for preventing the unsustainable exploitation of natural reserves.This review aims to highlight the diverse benefits these microorganisms offer across various domains while presenting an exciting opportunity to advance sustainable agriculture and firstly establishes a connection between nutrient solubilization and pesticide degradation mediated by microorganisms.It also offers a comprehensive bibliographic review of the application of plant growth-promoting microorganisms for solubilizing nutrients,such as P,K,and Zn,and degrading pesticides as well.
基金supported by the National Natural Science Foundation of China(No.52070019).
文摘Wetlands are vital ecosystems that perform essential functions such as climate regulation,environmental purification,material circulation and energy flow.They play an essential role in global biogeochemical cycles,driven primarily by microorganisms.Understanding the distribution of wetland microorganisms across different temperature zones is key to comprehending their ecological roles.A meta-analysis of 704 wetland soil samples on microbial communities was conducted,using statistical methods like analysis of variance,principal component analysis,non-metric nultidimensional scaling,and structural equation modeling to examine biogeography and diversity across temperature zones.Our findings revealed a clear latitudinal diversity gradient pattern,with the highest microbial abundance in the tropics and the lowest in the southern temperate zone,which differed significantly from other temperature zones.Proteobacteria(37.76%-51.04%),Acidobacteria(5.11%-30.70%)and Bacteroidetes(3.43%-16.16%)dominanted the bacterial communities.Notably,the southern temperate zone showed significant variations,with a higher prevalence of Acidobacteria(30.07%).To investigate the causes of this variability,we screened 177 core microbiome and identified latitude as the core environmental factor influencing microbial community composition.Moreover,soil microorganisms exhibited strong nitrogen cycling potentials(particularly nirD and nirB)and carbon cycling potentials(especially accA),with gene abundances showing little variation across temperature zones.Wetland bacterial communities also demonstrated high stability,with average variation degree index values ranging from 0.1 to 0.3.Our results improve the understanding of the diversity and biogeographic mechanisms of wetland bacterial communities and hold significant implications for the management and conservation of wetlands.
基金supported by the Beijing Natural Science Foundation(L242149)Research Project on High Quality Development of Hospital Pharmacy,National Institute of Hospital Administration,NHC,China(NIHAYS2332)+1 种基金National High Level Hospital Clinical Research Funding(BJ-2023-199)Capital Funds for Health Improvement and Research(CFH)(2024-1-4052).
文摘Objective To examine national trends in antibiotic consumption and antimicrobial resistance(AMR)among six WHO-priority bacterial pathogens in China from 2016 to 2022.Methods This ecological study analyzed national and provincial data from the China Antibacterial Resistance Surveillance System(CARSS)and the National Hospital Information Network.Beta regression models assessed temporal trends,and hierarchical models evaluated associations between antibiotic use and resistance.Results From 2016 to 2022,carbapenem resistance in Acinetobacter baumannii and Pseudomonas aeruginosa,and vancomycin resistance in Enterococcus faecium and E.faecalis significantly declined(β<0,P<0.010),while carbapenem-resistant Klebsiella pneumoniae increased(β=0.081,P<0.001).Nationwide antibiotic consumption rose across 10 major classes.Positive associations were found between carbapenem use and resistance in A.baumannii(z=2.719,P=0.007)and P.aeruginosa(z=3.241,P=0.001),and between vancomycin use and resistance in E.faecium(z=4.510,P=0.001)and E.faecalis(z=3.210,P=0.001).Conclusion Carbapenem-resistant K.pneumoniae increased significantly in China,while other resistant pathogens declined.Resistance patterns were linked to the use of multiple antibiotic classes,underscoring the need for strengthened antibiotic stewardship and surveillance.
基金supported by the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202416)the Science and Technology Major Projects of Hubei Province(2023BBA002)the National Natural Science Foundation of China grants(32441047,32441046 and 32471627)。
文摘Rhizobial inoculation in soybean is an effective strategy in sustainable agriculture to reduce chemical fertilizer application and to increase crop production.It not only provides nitrogen sources for host plants but also improves the rhizosphere soil environment.However,the inoculation efficiency of rhizobia remains to be improved.In this study,we investigated the nodulation efficiency of Bradyrhizobium and Sinorhizobium strains under different soil conditions and evaluated their impacts on the rhizocompartment bacterial community.We found that inoculation with Bradyrhizobium diazoefficiens UASD 110 increased the number of soybean nodules in acidic soil,while Sinorhizobium fredii CCBAU 45436 was more effective in alkaline soil.However,inoculation with neither strain significantly affected nodulation in neutral soil.Then,we demonstrated that UASD 110 was more competitive in nodulation than CCBAU 45436,which was related to its higher abundance in the rhizosphere.Furthermore,we showed that while single inoculation with UASD 110 or CCBAU 45436 failed to alter the bacterial diversity,these two strains differentially influenced the rhizosphere microbial composition.Finally,we identified the main rhizosphere microorganisms that were affected by these two strains.Our findings revealed that the nodulation capacity of rhizobia and their colonization of rhizosphere and nodules are soil-type dependent,yet their impact on the rhizobacterial community exhibited consistent patterns.These findings provide valuable insights into optimizing rhizobial inoculation strategies to enhance nitrogen fixation efficiency.
基金supported by the National Natural Science Foundation of China(22378166)the Basic Research Program of Jiangsu and Jiangsu Basic Research Center for Synthetic Biology(BK20233003)+1 种基金the Fundamental Research Funds for the Central Universities(JUSRP622001)the Open Funding Project of Key Laboratory of Industrial Biotechnology Ministry of Education(KLIB-KF202403).
文摘Formate bioconversion plays a crucial role in achieving renewable resource utilization and green and sustainable development,as it helps convert formate to biofuels and biochemicals.However,to tap the full potential of formate bioconversion,it is important to identify the most appropriate microbial hosts,design the most promising formate assimilation pathways,and develop the most efficient formate assimilation cell factories.Here,we summarize the formatotrophic microorganisms capable of assimilating formate into building blocks of cell growth and analyze the characteristics of formate assimilation pathways for transmitting formate into central carbon metabolism.Furthermore,we discuss microbial engineering strategies to improve the efficiency of formate utilization for producing high-value bioproducts.Finally,we highlight the key challenges of formate bioconversion and their possible solutions to advance the formate bioeconomy and biomanufacturing.
基金supported by the National Natural Science Foundation of China(Grant nos.42476264,41976224).
文摘Small RNAs(sRNAs)are important non-coding RNAs that usually play crucial roles in gene expression at the post-transcriptional level.The sRNAs have mostly been investigated in model microorganisms such as Escherichia coli and some pathogens.Nevertheless,microbial sRNAs from extreme environments such as the polar regions and deep sea have recently been discovered and analyzed for their unique roles in stress response,metabolic regulation and adaptation to extreme environments.These sRNAs fine-tune gene expression during oxidative and radiation stress,and modulate temperature and osmotic pressure responses.Representative sRNAs and their functions in thermophilic,psychrophilic,halophilic and radiation-tolerant bacteria are summarized in this review.Despite challenges in sample collection,RNA isolation,and functional annotation,the study of sRNAs in extreme environments provides opportunities for discovering novel regulatory mechanisms,applying them to biotechnology,and advancing our understanding of evolutionary adaptations.Looking ahead,high-throughput sequencing,synthetic biology,and multi-omics integration will bring new breakthroughs in discovering novel sRNAs and their functions and regulatory mechanisms.Such advancements are poised to enable comprehensive characterization of sRNA-mediated regulatory networks in extremophiles and unlock their biotechnological potential through mechanism-driven applications.
基金supported by project OLP116.CSIR-NBRI allotted the manuscript number CSIR-NBRI_MS/2025/06/15。
文摘The excessive reliance on chemical inputs for managing soil nutrients and pathogens has raised concerns about their long-term sustainability and environmental impact.In contrast,the use of soil microbes offers an eco-friendly and efficient alternative for improving soil fertility and plant growth.Beneficial microorganisms,including plant growth-promoting rhizobacteria(PGPR),mycorrhizal fungi,and other soil organisms,play pivotal roles in nutrient cycling,organic matter decomposition,and nutrient availability improvement.This review explores the potential of leveraging microbial resources for sustainable soil nutrient management and resilient crop production.It delves into the intricate interactions between host plants and PGPR,particularly under nutrient-limited and fluctuating environmental conditions,with a focus on the molecular signaling pathways and mechanisms regulating these relationships.Furthermore,it emphasizes the role of advanced techniques and PGPR-responsive microRNAs to uncover the functional capabilities of microbial communities and their dynamic interactions with plants.These approaches pave the way for developing innovative,microbe-based strategies to optimize nutrient use efficiency,reduce dependency on synthetic fertilizers,and support sustainable agricultural practices.
基金supported by the National Natural Science Foundation of China(No.51974128)the National Key Research and Development Program of China(No.2023YFC3009105)。
文摘Early prevention and control of coal spontaneous combustion have emerged as a critical research area in coal mine safety.Due to their sustainability and environmental friendliness,microorganisms have gained attention.A filamentous fungus was collected in the coal mine and identified as Absidia spinosa.Results indicated that the mycelium effectively covered and repaired many coal pores.The oxygen consumption ratio of A.spinosa was higher in coal-containing environments than in coal-free conditions.The fungus significantly impacted aliphatic functional groups,disrupting bridging bonds and side chains connected to aromatic structures and reducing the relative content of C—O bonds.Additionally,A.spinosa increases the ignition temperature by 25.34℃.The total heat release was decreased by approximately 32.58%,and the activation energies were increased.The genome of Absidia spinosa revealed genes related to oxygen consumption,small molecule degradation,and secretion of metabolic products,such as those annotated under GO ID:0140657,etc.The pathways involved in the degradation of small organic molecules(e.g.,ko00626,etc.),carbon fixation,and nitrogen cycling,all linked to coal decomposition.Through oxygen consumption and the alteration of coal-active structures,A.spinosa effectively inhibits CSC,providing an experimental basis for exploring eco-friendly biological control methods in the goaf.
基金Funded by the National Key R&D Program of China(No.2023YFC3806100)the National Nature Science Foundation of China(Nos.52278269,52278268,52178264,and 52108238)+2 种基金the Tianjin Outstanding Young Scholars Science Fund Project(No.22JCJQJC00020)the Key Project of Tianjin Natural Science Foundation(No.23JCZDJC00430)the Joint Research Center of China and Foreign Countries Special Fund of Tianjin Innovation Platform(No.24PTLYHZ00240)。
文摘Sporosarcina pasteurii was employed as the strain,with an in-situ magnetization construction,to obtain magnetic microorganisms and oriented self-healing mortar specimens based on them.The magnetic field was used to achieve the directional migration of magnetic microorganisms during the oriented selfhealing of mortar cracks,improving the rate of self-healing of cracks.The experimental results demonstrate that the magnetic microorganisms are composed of Fe_(3)O_(4)nanosheets attached to the surface of Sporosarcina pasteurii,whose mineralization products are comprised of vaterite primarily.Compared with the pure microbial group,the magnetic microbial group exhibits a faster repair rate,shortening the repair time required to achieve an area repair efficiency of over 90%from 28 days to 14 days,thereby doubling the repair rate.Meanwhile,the area repair efficiency of the magnetic microbial group at 7,14,and 28 days are increased by 50.3%,11.2%,and 4.6%,respectively,compared to the pure microbial group,which are due to the magnetic microorganisms'superior directional migration and mineralization ability,exceeding that of the ordinary microorganisms.
文摘The present study investigates the flow,heat,and mass transfer analysis in the bioconvection of nanofluid containing motile gyrotactic microorganisms through a semi-porous curved oscillatory channel with a magnetic field.These microorganisms produce density gradients by swimming,which induces macroscopic convection flows in the fluid.This procedure improves the mass and heat transfer,illustrating the interaction between biological activity and fluid dynamics.Furthermore,instead of considering traditional Fourier's and Fick's law the energy and concentration equations are developed by incorporating Cattaneo-Christov double diffusion theory.Moreover,to examine the influence of thermophoresis and Brownian diffusions in the fluid we have adopted the Buongiorno nanofluid model.Due to the oscillation of the surface of the channel,the mathematical development of the considered flow problem is obtained in the form of partial differential equations via the curvilinear coordinate system.The convergent series solution of the governing flow equations is obtained after applying the homotopy analysis method(HAM).The effects of different pertinent flow parameters on velocity,motile microorganism density distribution,concentration,pressure,temperature,and skin friction coefficient are examined and discussed in detail with the help of graphs and tables.It is observed during the current study that the density of microorganisms is enhanced for higher values of Reynolds number,Peclet number,radius of curvature variable,and Lewis number.
基金the support of the National Natural Science Foundation of China(No.42107247)the National Key Research and Development Project(No.2022YFD1901605)+1 种基金the Natural Science Foundation of Sichuan Province(Nos.2025YFHZ0142 and 2024NSFSC0800)the Tobacco Science Foundation of Sichuan Province(No.SCYC202407)。
文摘Soil microorganisms and labile soil organic carbon(SOC)fractions are essential factors affecting greenhouse gas(GHG)emissions in paddy fields.However,the effects of labile SOC fractions and microorganisms on GHG emissions from flooding to drying after organic fertilizer replacing for chemical fertilizer remain unclear.Here,a long-term experiment was conducted with four treatments:chemical fertilization only(control),organic fertilizer substituting 25%of chemical N fertilizer(NM1),50%of chemical N fertilizer(NM2),and NM2combined with crop straw(NMS).GHG emissions were monitored,and soil samples were collected to determine labile SOC fractions and microorganisms.Results revealed the GHG emissions in the NM2 significantly increased by 196.88%from flooding to drying,mainly due to the higher CO_(2) emissions.The GHG emissions per kg of C input in NMS was the lowest with the value of 9.17.From flooding to drying,organic fertilizer application significantly increased the readily oxidizable organic carbon(ROC)contents and C lability;the NM2 and NMS dramatically increased the SOC and non-readily oxidizable organic carbon(NROC).The bacterial communities showed significant differences among different treatments in the flooding,while the significant difference was only found between the NMS and other treatments in the drying.From flooding to drying,changing soil moisture conditions causes C fractions and microbial communities to jointly affect carbon emissions,and the NMS promoted carbon sequestration and mitigated GHG emissions.Our findings highlight the importance of the labile SOC fractions and microorganisms linked to GHG emissions in paddy fields.
