Damage to the adult mammalian central nervous system （CNS） often results in persistent neurological deficits with limited recovery of functions. The past decade has seen in- creasing research efforts in neural regen...Damage to the adult mammalian central nervous system （CNS） often results in persistent neurological deficits with limited recovery of functions. The past decade has seen in- creasing research efforts in neural regeneration research with the ultimate goal of achieving functional recovery. Many studies have focused on prevention of further neural damage and restoration of functional connections that are com- promised after iniurY or pathological damage.展开更多
In recent years,microfluidic systems have evolved to incorporate increasingly complex multi-layer and multi-materialstructures.While conventional 2-dimensional microfluidic systems are typically fabricated with lithog...In recent years,microfluidic systems have evolved to incorporate increasingly complex multi-layer and multi-materialstructures.While conventional 2-dimensional microfluidic systems are typically fabricated with lithographic techniques,the increase in system complexity necessitates a more versatile set of fabrication techniques.Similarly,although 3Dprinting can easily produce intricate microfluidic geometries,integrating multiple membranes and electrodecomponents remains challenging.This study proposes a toolkit for fabricating free-standing 3-dimensionalmicrofluidic systems for biomedical devices,incorporating flow channels,electrodes,and membranes.The fabricationtechniques include molding separation using 3D printed molds,laser-based processing,and component assembly,each achieving micron resolution.Here,we introduce a novel approach to integrate membranes into microfluidics bydirectly curing elastomer-based microfluidics with the membrane through replica molding,while preservingmembrane functionality by effectively removing elastomer residues through reactive ion etching.The resultingmembrane-elastomer microfluidic component significantly simplifies the assembly of intricate microfluidic systems,reducing the device size to millimeter dimensions,suitable for implantable applications.The toolkit’s versatility isdemonstrated by a redox flow iontophoretic drug delivery prototype at the millimeter scale,featuring two electrodes,four membranes,and four microfluidic channels.展开更多
CONSPECTUS:The microfluidic biochemical/immunoassay systems typically consist of microfluidic chips,fluid driving devices,and detection components.The core of the system is the microfluidic chips based on microfluidic...CONSPECTUS:The microfluidic biochemical/immunoassay systems typically consist of microfluidic chips,fluid driving devices,and detection components.The core of the system is the microfluidic chips based on microfluidic technology,which are typically constructed with nonresponsive materials such as silicon,glass,and rigid plastics,thus requiring complex external air/liquid pumps to manipulate the samples.The external equipment renders the microfluidic systems cumbersome and increases the risk of biosample contamination.展开更多
基金supported by grants from NIH National Institute of Mental Health MH085267National Institute of Neurological Disorders and Stroke NS060017
文摘Damage to the adult mammalian central nervous system （CNS） often results in persistent neurological deficits with limited recovery of functions. The past decade has seen in- creasing research efforts in neural regeneration research with the ultimate goal of achieving functional recovery. Many studies have focused on prevention of further neural damage and restoration of functional connections that are com- promised after iniurY or pathological damage.
文摘In recent years,microfluidic systems have evolved to incorporate increasingly complex multi-layer and multi-materialstructures.While conventional 2-dimensional microfluidic systems are typically fabricated with lithographic techniques,the increase in system complexity necessitates a more versatile set of fabrication techniques.Similarly,although 3Dprinting can easily produce intricate microfluidic geometries,integrating multiple membranes and electrodecomponents remains challenging.This study proposes a toolkit for fabricating free-standing 3-dimensionalmicrofluidic systems for biomedical devices,incorporating flow channels,electrodes,and membranes.The fabricationtechniques include molding separation using 3D printed molds,laser-based processing,and component assembly,each achieving micron resolution.Here,we introduce a novel approach to integrate membranes into microfluidics bydirectly curing elastomer-based microfluidics with the membrane through replica molding,while preservingmembrane functionality by effectively removing elastomer residues through reactive ion etching.The resultingmembrane-elastomer microfluidic component significantly simplifies the assembly of intricate microfluidic systems,reducing the device size to millimeter dimensions,suitable for implantable applications.The toolkit’s versatility isdemonstrated by a redox flow iontophoretic drug delivery prototype at the millimeter scale,featuring two electrodes,four membranes,and four microfluidic channels.
基金financially supported by the National Natural Science Foundation of China(51927805,52233001)the Innovation Program of Shanghai Municipal Education Commission(2023ZKZD07).
文摘CONSPECTUS:The microfluidic biochemical/immunoassay systems typically consist of microfluidic chips,fluid driving devices,and detection components.The core of the system is the microfluidic chips based on microfluidic technology,which are typically constructed with nonresponsive materials such as silicon,glass,and rigid plastics,thus requiring complex external air/liquid pumps to manipulate the samples.The external equipment renders the microfluidic systems cumbersome and increases the risk of biosample contamination.
