Effective antibacterial property and long-term mechanical support are essential for the repair of complex ab-dominal wall defects associated with infection.However,clinically available repair materials often fail to m...Effective antibacterial property and long-term mechanical support are essential for the repair of complex ab-dominal wall defects associated with infection.However,clinically available repair materials often fail to meet these requirements,resulting in high surgical failure rate and complications.In this study,an asymmetric porous composite hydrogel patch(cCS/PVA@BAC)with antibacterial,anti-adhesion,pro-healing,and durable mechan-ical support properties is designed for the efficient repair of contaminated abdominal wall defects.By stepwise phase-conversion and soaking method,robust and stable polyvinyl alcohol hydrogel(PVAH)is integrated with the biocompatible multicomponent hydrogel made of chitosan and carboxymethyl chitosan(cCS),and benza-lkonium chloride(BAC)is loaded to enhance the antibacterial property.The cCS layer of cCS/PVA@BAC has an extracellular matrix-like porous structure,which can promote fibroblasts adhesion and wound healing.In contrast,the PVAH layer on the other side with a smooth and dense structure,which can reduce fibroblasts adhesion and prevent visceral adhesion.In addition,the composite hydrogel patch has good anti-swelling and anti-deformation properties as well as stable mechanical strength,thus can withstand high intraperitoneal pres-sure in the wet internal microenvironment.The loaded BAC can efficiently kill bacteria and improve the local inflammatory microenvironment.With these advantages,cCS/PVA@BAC can significantly reduce inflammation,promote tissue remodeling,and accelerate the healing of contaminated abdominal wall defects in the rat model.These findings suggest a potential use of multifunctional hydrogel patch as an ideal material for effective repair of contaminated soft tissue defects.展开更多
基金supported by the projects of the National Key R&D Program of China(2021YFC2101700)National Natural Science Foundation of China(52372057,22371312)+4 种基金Guangdong Major Project of Basic and Applied Basic Research(2023B0303000025)Sun Yat-sen University Clinical Research 5010 Program(2017008)Medical Scientific and Technical Foundation of Guangdong Province(A2023085)Fundamental Research Funds for the Central Universities,Sun Yat-sen University(23yxqntd002)Guangdong Basic Research Center of Excellence for Functional Molecular Engineering.
文摘Effective antibacterial property and long-term mechanical support are essential for the repair of complex ab-dominal wall defects associated with infection.However,clinically available repair materials often fail to meet these requirements,resulting in high surgical failure rate and complications.In this study,an asymmetric porous composite hydrogel patch(cCS/PVA@BAC)with antibacterial,anti-adhesion,pro-healing,and durable mechan-ical support properties is designed for the efficient repair of contaminated abdominal wall defects.By stepwise phase-conversion and soaking method,robust and stable polyvinyl alcohol hydrogel(PVAH)is integrated with the biocompatible multicomponent hydrogel made of chitosan and carboxymethyl chitosan(cCS),and benza-lkonium chloride(BAC)is loaded to enhance the antibacterial property.The cCS layer of cCS/PVA@BAC has an extracellular matrix-like porous structure,which can promote fibroblasts adhesion and wound healing.In contrast,the PVAH layer on the other side with a smooth and dense structure,which can reduce fibroblasts adhesion and prevent visceral adhesion.In addition,the composite hydrogel patch has good anti-swelling and anti-deformation properties as well as stable mechanical strength,thus can withstand high intraperitoneal pres-sure in the wet internal microenvironment.The loaded BAC can efficiently kill bacteria and improve the local inflammatory microenvironment.With these advantages,cCS/PVA@BAC can significantly reduce inflammation,promote tissue remodeling,and accelerate the healing of contaminated abdominal wall defects in the rat model.These findings suggest a potential use of multifunctional hydrogel patch as an ideal material for effective repair of contaminated soft tissue defects.
