γ-Aminobutyric acid(GABA),a natural“neuro-vitamin”,exerts an irreplaceable role in modulating gut microbiota,thereby influencing brain function and host behavior.However,there are ethical and safety challenges base...γ-Aminobutyric acid(GABA),a natural“neuro-vitamin”,exerts an irreplaceable role in modulating gut microbiota,thereby influencing brain function and host behavior.However,there are ethical and safety challenges based on clinical trials and model animals.Herein,an in vitro fecal slurry fermentation model was constructed to explore the impacts of GABA and its precursor monosodium glutamate(MSG)on the regulation of human intestinal microorganisms.Both GABA(25.43%–41.57%)and MSG(28.72%–49.59%)supplementation promoted the relative abundance of Actinobacteria phylum compared to the Ctrl group(18.68%),particularly characterized by Bifidobacterium representation in fecal.Notably,high levels of GABA and MSG addition significantly reduced the abundance of Bacteriodota by 65.15%and 77.61%.respectively.Analysis of short-chain fatty acids(SCFAs)suggested that high levels of GABA supplementation significantly increased the production of total acid and acetic acid through fermentation.Moreover,the Spearman correlation analysis of high GABA treatment group showed a significant positive association between acetic acid,isobutyric acid and 2-methylbutyric acid with the genera Bifidobacterium,Subdoligranulum and Lachnoclostridium after fecal fermentation,respectively.The in vitro colonic fermentation model established in our study may offer valuable views for future studies on the GABA-gut microbiota interaction under the background of nutritional intervention treatment.展开更多
Aiming to investigate the impact of different stocking densities on the ability of Pacific white shrimp(Litopenaeus vannamei)to utilize Chlorella sorokiniana(CHL),a 3×2 factorial design stocking experiment was us...Aiming to investigate the impact of different stocking densities on the ability of Pacific white shrimp(Litopenaeus vannamei)to utilize Chlorella sorokiniana(CHL),a 3×2 factorial design stocking experiment was used in this study.Specifically,shrimp was fed with two dietary protein sources(fishmeal[FM]and CHL)at low(LSD;100 per m^(3)),medium(MSD;200 per m^(3))and high(HSD;300 per m^(3))stocking densities for 8 weeks.The growth performance and resistance to Vibrio parahaemolyticus(1.0×10^(7) CFU/mL)of shrimp decreased with the increase of stocking density,but dietary CHL improved this result.Differences between the CHL and FM groups for V.parahaemolyticus resistance were significant only under high-density conditions(P<0.05).Significant interactions between stocking density and protein source were found on the activities of catalase(CAT),superoxide dismutase(SOD)and phenol oxidase(PO),and the contents of malondialdehyde(MDA)in the hepatopancreas and the activities of intestinal amylase,most of which were significantly different between CHL and FM groups only at high stocking density(P<0.05).Analysis of 16S rDNA sequencing showed that dietary CHL increased the alpha diversity of intestinal microbiota,inhibited the colonization of pathogenic bacteria and enhanced the abundance of beneficial bacteria.Transcriptomic results showed that at high stocking densities,differentially expressed genes(DEGs)in the FM vs CHL group were mostly upregulated and primarily enriched in immune and metabolic related pathways including Toll,immune deficiency(Imd)and glycolysis–gluconeogenesis pathways.Pearson correlation analysis revealed significant correlation between the top ten intestinal bacteria at the genus level and markedly enriched DEGs,also more were detected under high density situations.In conclusion,CHL has great potential as a novel protein source in the intensive farming of shrimp.展开更多
Quality consistency of high-temperature Daqu(HTD)is crucial for optimizing the production and sensory attributes of Chinese sauce-flavor Baijiu.It is also essential for tracking microbiota succession,enzymatic activit...Quality consistency of high-temperature Daqu(HTD)is crucial for optimizing the production and sensory attributes of Chinese sauce-flavor Baijiu.It is also essential for tracking microbiota succession,enzymatic activity,and metabolic profiles during continuous HTD production.Using multi-phase detection technologies,including high-throughput sequencing and metabolomic analysis,the effects of Muqu(mature HTD)addition and its attributes on HTD properties over multiple production rounds were examined.The results demonstrated that Muqu significantly influenced the microbial community of HTD,particularly increasing the relative abundance of key functional microorganisms,such as Kroppenstedtia and Thermomyces,thereby improving enzymatic activities which were saccharification,fermentation,and esterification activity etc,and optimizing the metabolic profile of HTD.Furthermore,the study revealed that the combined effects of Muqu attributes,production site,and process heterogeneity significantly influence inter-round variations in HTD properties,highlighting the importance of controlling production parameters to achieve consistent quality.These findings provide novel insights into the role of Muqu in shaping HTD microbial communities and metabolic functionally,offering a scientific foundation for optimizing industrial-scale HTD production and improving quality management strategies.展开更多
基金supported financially by the National Key Research and Development Program of China[2022YFD2100703]the National Natural Science Foundation of China[32402117]+1 种基金the Basic Research Business Fee Project for Provincial Universities[QRK23001]Zhejiang Association for Science and Technology Youth Talent Support Project.
文摘γ-Aminobutyric acid(GABA),a natural“neuro-vitamin”,exerts an irreplaceable role in modulating gut microbiota,thereby influencing brain function and host behavior.However,there are ethical and safety challenges based on clinical trials and model animals.Herein,an in vitro fecal slurry fermentation model was constructed to explore the impacts of GABA and its precursor monosodium glutamate(MSG)on the regulation of human intestinal microorganisms.Both GABA(25.43%–41.57%)and MSG(28.72%–49.59%)supplementation promoted the relative abundance of Actinobacteria phylum compared to the Ctrl group(18.68%),particularly characterized by Bifidobacterium representation in fecal.Notably,high levels of GABA and MSG addition significantly reduced the abundance of Bacteriodota by 65.15%and 77.61%.respectively.Analysis of short-chain fatty acids(SCFAs)suggested that high levels of GABA supplementation significantly increased the production of total acid and acetic acid through fermentation.Moreover,the Spearman correlation analysis of high GABA treatment group showed a significant positive association between acetic acid,isobutyric acid and 2-methylbutyric acid with the genera Bifidobacterium,Subdoligranulum and Lachnoclostridium after fecal fermentation,respectively.The in vitro colonic fermentation model established in our study may offer valuable views for future studies on the GABA-gut microbiota interaction under the background of nutritional intervention treatment.
基金supported by the Special Projects in Key Fields of Ordinary University in Guangdong Province (2023ZDZX4010)General Program of Natural Science Foundation of Guangdong Province,China (Grant No.2022A 1515012325)Postgraduate Education Innovation Project of Guangdong Ocean University in 2023 (Grant No.202336).
文摘Aiming to investigate the impact of different stocking densities on the ability of Pacific white shrimp(Litopenaeus vannamei)to utilize Chlorella sorokiniana(CHL),a 3×2 factorial design stocking experiment was used in this study.Specifically,shrimp was fed with two dietary protein sources(fishmeal[FM]and CHL)at low(LSD;100 per m^(3)),medium(MSD;200 per m^(3))and high(HSD;300 per m^(3))stocking densities for 8 weeks.The growth performance and resistance to Vibrio parahaemolyticus(1.0×10^(7) CFU/mL)of shrimp decreased with the increase of stocking density,but dietary CHL improved this result.Differences between the CHL and FM groups for V.parahaemolyticus resistance were significant only under high-density conditions(P<0.05).Significant interactions between stocking density and protein source were found on the activities of catalase(CAT),superoxide dismutase(SOD)and phenol oxidase(PO),and the contents of malondialdehyde(MDA)in the hepatopancreas and the activities of intestinal amylase,most of which were significantly different between CHL and FM groups only at high stocking density(P<0.05).Analysis of 16S rDNA sequencing showed that dietary CHL increased the alpha diversity of intestinal microbiota,inhibited the colonization of pathogenic bacteria and enhanced the abundance of beneficial bacteria.Transcriptomic results showed that at high stocking densities,differentially expressed genes(DEGs)in the FM vs CHL group were mostly upregulated and primarily enriched in immune and metabolic related pathways including Toll,immune deficiency(Imd)and glycolysis–gluconeogenesis pathways.Pearson correlation analysis revealed significant correlation between the top ten intestinal bacteria at the genus level and markedly enriched DEGs,also more were detected under high density situations.In conclusion,CHL has great potential as a novel protein source in the intensive farming of shrimp.
文摘Quality consistency of high-temperature Daqu(HTD)is crucial for optimizing the production and sensory attributes of Chinese sauce-flavor Baijiu.It is also essential for tracking microbiota succession,enzymatic activity,and metabolic profiles during continuous HTD production.Using multi-phase detection technologies,including high-throughput sequencing and metabolomic analysis,the effects of Muqu(mature HTD)addition and its attributes on HTD properties over multiple production rounds were examined.The results demonstrated that Muqu significantly influenced the microbial community of HTD,particularly increasing the relative abundance of key functional microorganisms,such as Kroppenstedtia and Thermomyces,thereby improving enzymatic activities which were saccharification,fermentation,and esterification activity etc,and optimizing the metabolic profile of HTD.Furthermore,the study revealed that the combined effects of Muqu attributes,production site,and process heterogeneity significantly influence inter-round variations in HTD properties,highlighting the importance of controlling production parameters to achieve consistent quality.These findings provide novel insights into the role of Muqu in shaping HTD microbial communities and metabolic functionally,offering a scientific foundation for optimizing industrial-scale HTD production and improving quality management strategies.
