The application of microbial technology to mineral processing hasbeen reviewed with respect to the bioleaching of metals fromminerals, the influence of biotreatment on flotation, the biobeneficication of nonmetallic m...The application of microbial technology to mineral processing hasbeen reviewed with respect to the bioleaching of metals fromminerals, the influence of biotreatment on flotation, the biobeneficication of nonmetallic minerals, and the biotreatment for minewaste reclamation. The application of microbial technology to mineralprocess- ing has shown the advantages of high efficiency, low en-Ergy and reagent consumption, low capital and operating Costs, andlow pollution of the environment.展开更多
At present, the development of microbial technology in China is relatively rapid, and its application in fermentation pharmacy is relatively mature, breaking through the bottleneck of traditional pharmacy. Combined wi...At present, the development of microbial technology in China is relatively rapid, and its application in fermentation pharmacy is relatively mature, breaking through the bottleneck of traditional pharmacy. Combined with relevant theories, this paper introduces the key role of microbial technology in fermentation pharmacy, and analyzes the specific application, in order to better play the role of microbial technology, realize the innovation and optimization of pharmaceutical technology, and accumulate valuable experience for the development of China's pharmaceutical industry.展开更多
Plant diseases affect the cultivation of Chinese herbal medicines,while traditional chemical pesticides have many drawbacks such as environmental pollution,health risks and disruption of ecological balance.Microbiocon...Plant diseases affect the cultivation of Chinese herbal medicines,while traditional chemical pesticides have many drawbacks such as environmental pollution,health risks and disruption of ecological balance.Microbiocontrol has gradually appeared in public view,and its application has become increasingly extensive.This paper reviews the disease-causing species of medicinal plants,including fungal,bacterial,nematode,viral and parasitic pests,and reviews the diseases caused by microorganisms in traditional Chinese medicine planting and their biological control by consulting Sciencedirect databases and Web of Science databases.4667 related articles were found,of which 552 were related to microbiocontrol technology and cultivation of traditional Chinese medicines.This review provides a reference for the green planting technology of traditional Chinese medicine.展开更多
Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and ...Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins,that are widely applied as the core elements in the field of biosensors due to their advantages,including enhanced stability,high yield,good safety,expression of larger and more complex proteins.To further promote the performance of biosensors,the biomineralized microbial surface display technology was proposed.This review summarized the different microbial surface display systems and the biomineralized surface display systems,where the mechanisms of surface display and biomineralization were introduced.Then we described the recent progress of their applications on biosensors for different types of detection targets.Finally,the outlooks and tendencies were discussed and forecasted with the expectation to provide some general functions and enlightenments to this aspect of research.展开更多
Biosurfactants are biologically active metabolites, and the efficiency of direct screening of new biosurfactants from nature using traditional methods is low, which should be enhanced in the following studies by adopt...Biosurfactants are biologically active metabolites, and the efficiency of direct screening of new biosurfactants from nature using traditional methods is low, which should be enhanced in the following studies by adopting advanced biotechnologies. Rapid development and wide application of microbial culture independent methods, such as metagenomics, metatranscriptomics, metaproteomics and metabonomics, etc., contributes to quickly and precisely screening of novel biological surfactants. We mainly represented the current status of research and applications of biosurfactants in the remediation of petrochemical polluted environment, and also prospected avenues for future research.展开更多
Synthetic microbial community has widely concerned in the fields of agriculture,food and environment over the past few years.However,there is little consensus on the method to synthetic microbial community from constr...Synthetic microbial community has widely concerned in the fields of agriculture,food and environment over the past few years.However,there is little consensus on the method to synthetic microbial community from construction to functional verification.Here,we review the concept,characteristics,history and applications of synthetic microbial community,summarizing several methods for synthetic microbial community construction,such as isolation culture,core microbiome mining,automated design,and gene editing.In addition,we also systematically summarized the design concepts,technological thresholds,and applicable scenarios of various construction methods,and highlighted their advantages and limitations.Ultimately,this review provides four efficient,detailed,easy-to-understand and-follow steps for synthetic microbial community construction,with major implications for agricultural practices,food production,and environmental governance.展开更多
Gastric cancer(GC)is one of the most common cancers worldwide particularly in Asian populations,and certain diets have been associated with increased risk of GC.Recent advances in microbial profiling technology have f...Gastric cancer(GC)is one of the most common cancers worldwide particularly in Asian populations,and certain diets have been associated with increased risk of GC.Recent advances in microbial profiling technology have facilitated investigations on microbes residing on the gastric mucosa and increasing evidence has revealed the critical roles of non-Helicobacter pylori gastric microbes in gastric tumorigenesis.On the other hand,diets can affect microbial communities,causing compositional and functional shift of the microbiota.In this review,we summarize the influence of various diets including processed meat,salt-preserved food,high-fat diet,and alcohol on the development and progression of GC.We also explore microbial metabolites and host-microbe interactions in gastric tumorigenesis,alongside dietary interventions targeting the microbiota for the prevention and management against GC.展开更多
50%of pharmaceuticals and 25%of herbicides used worldwide are chiral.Each enantiomer has a unique toxicity and biodegradation profile,affecting differently to organisms.Chirality plays a key role in the behavior of th...50%of pharmaceuticals and 25%of herbicides used worldwide are chiral.Each enantiomer has a unique toxicity and biodegradation profile,affecting differently to organisms.Chirality plays a key role in the behavior of these emerging contaminants(ECs)in terms of their pharmacological or herbicidal activity,but this peculiarity is often overlooked in environmental research.The complexity of chiral ECs is underestimated,as the varying sensitivity of biological systems to enantiomers is rarely considered.Biofilters can promote the activity of specific microbial communities,facilitating the degradation of ECs,due to the greater interaction between water and microorganisms and their compact design.Here,we show that an electroactive biofilter can alter the chirality of drugs and herbicides in wastewater treatment,impacting their removal and toxicity.The electrochemical biofilter(BioeF)removed 80%of pharmaceuticals and 50e75%of herbicides,outperforming the conventional filter(ConF).BioeF also showed greater chiral alterations and lower ecotoxicity.This work provides the first evidence of a relationship between changes in contaminant chirality and detoxification capacity,enhanced by electroactive systems.The increased microbial activity observed in the BioeF suggests that bioelectrochemical systems offer a valuable advance for ECs removal and ecotoxicity reduction,addressing the environmental challenge posed by ECs.展开更多
It has been recently suggested that Alcaligenes use a previously unknown pathway to convert ammonium into dinitrogen gas(Dirammox)via hydroxylamine(NH2OH).This fact alone already implies a significant decrease in the ...It has been recently suggested that Alcaligenes use a previously unknown pathway to convert ammonium into dinitrogen gas(Dirammox)via hydroxylamine(NH2OH).This fact alone already implies a significant decrease in the aeration requirements for the process,but the process would still be dependent on external aeration.This work studied the potential use of a polarised electrode as an electron acceptor for ammonium oxidation using the recently described Alcaligenes strain HO-1 as a model heterotrophic nitrifier.Results indicated that Alcaligenes strain HO-1 requires aeration for metabolism,a requirement that cannot be replaced for a polarised electrode alone.However,concomitant elimination of succinate and ammonium was observed when operating a previously grown Alcaligenes strain HO-1 culture in the presence of a polarised electrode and without aeration.The usage of a polarised electrode together with aeration did not increase the succinate nor the nitrogen removal rates observed with aeration alone.However,current density generation was observed along a feeding batch test representing an electron share of 3%of the ammonium removed in the presence of aeration and 16%without aeration.Additional tests suggested that hydroxylamine oxidation to dinitrogen gas could have a relevant role in the electron discharge onto the anode.Therefore,the presence of a polarised electrode supported the metabolic functions of Alcaligenes strain HO-1 on the simultaneous oxidation of succinate and ammonium.展开更多
A laminar flow bioelectrochemical systems(BES)was designed and benchmarked using microbial anodes dominated with Geobacter spp.The reactor architecture was based on modeled flow fields,the resulting structure was 3D p...A laminar flow bioelectrochemical systems(BES)was designed and benchmarked using microbial anodes dominated with Geobacter spp.The reactor architecture was based on modeled flow fields,the resulting structure was 3D printed and used for BES manufacturing.Stratification of the substrate availability within the reactor channels led to heterogeneous biomass distribution,with the maximum biomass found mainly in the initial/middle channels.The anode performance was assessed for different hydraulic retention times while coulombic efficiencies of up to 100%(including also hydrogen recycling from the cathode)and current densities of up to 75 μA cm^(-2) at an anode surface to volume ratio of 1770 cm^(2) L^(-1) after 35 days were achieved.This low current density can be clearly attributed to the heterogeneous distributions of biomass and the stratification of the microbial community structure.Further,it was shown that time and space resolved analysis of the reactor microbiomes per channel is feasible using flow cytometry.展开更多
A novel microorganism embedding material was prepared to enhance the biological nitrogen removal through simultaneous nitrification and denitrification. Polyvinyl alcohol （PVA）, sodium alginate （SA） and cyclodextr...A novel microorganism embedding material was prepared to enhance the biological nitrogen removal through simultaneous nitrification and denitrification. Polyvinyl alcohol （PVA）, sodium alginate （SA） and cyclodextrin （CD） were used to compose gel bead with embedded activated sludge. The effects of temperature, CD addition and concentrations of PYA and SA on nitrogen removal were evaluated. Results show that the gel bead with CD addition at 30℃contributed to the highest nitrogen removal efficiency and nitrogen removal rate of 85.4% and 2.08 mg·（L·h）^-1, respectively. Meanwhile, negligible NO3^- and NO2^- were observed, proving the occurrence of simultaneous nitrification and denitrification. The High-Throughput Sequencing confirms that the microbial community mainly contained Comamonadaceae in the proportion of 61.3%. Overall, CD increased gel bead＇s porosity and resulted in the high specific endogenous respiration rate and high nitrogen removal efficiency, which is a favorable additional agent to the traditional embedding material.展开更多
基金Supported by the Natural Science Foundation of Hubei Province
文摘The application of microbial technology to mineral processing hasbeen reviewed with respect to the bioleaching of metals fromminerals, the influence of biotreatment on flotation, the biobeneficication of nonmetallic minerals, and the biotreatment for minewaste reclamation. The application of microbial technology to mineralprocess- ing has shown the advantages of high efficiency, low en-Ergy and reagent consumption, low capital and operating Costs, andlow pollution of the environment.
文摘At present, the development of microbial technology in China is relatively rapid, and its application in fermentation pharmacy is relatively mature, breaking through the bottleneck of traditional pharmacy. Combined with relevant theories, this paper introduces the key role of microbial technology in fermentation pharmacy, and analyzes the specific application, in order to better play the role of microbial technology, realize the innovation and optimization of pharmaceutical technology, and accumulate valuable experience for the development of China's pharmaceutical industry.
基金financially supported by National Nature Science Foundation of China(81973284)Scientific Research Foundation of the Education Department of Liaoning Province(LJKZ0944).
文摘Plant diseases affect the cultivation of Chinese herbal medicines,while traditional chemical pesticides have many drawbacks such as environmental pollution,health risks and disruption of ecological balance.Microbiocontrol has gradually appeared in public view,and its application has become increasingly extensive.This paper reviews the disease-causing species of medicinal plants,including fungal,bacterial,nematode,viral and parasitic pests,and reviews the diseases caused by microorganisms in traditional Chinese medicine planting and their biological control by consulting Sciencedirect databases and Web of Science databases.4667 related articles were found,of which 552 were related to microbiocontrol technology and cultivation of traditional Chinese medicines.This review provides a reference for the green planting technology of traditional Chinese medicine.
基金the National Natural Science Foundation of China(Grant No.21705087)Youth Innovation Team Project for Talent Introduction and Cultivation in Universities of Shandong Province(096-1622002)+2 种基金Research Foundation for Distinguished Scholars of Qingdao Agricultural University(663-1117015)the Postgraduate Innovation Program of Qingdao Agricultural University(QNYCX21069)the National Innovation Training Program for College Students(No.202210435030).
文摘Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins,that are widely applied as the core elements in the field of biosensors due to their advantages,including enhanced stability,high yield,good safety,expression of larger and more complex proteins.To further promote the performance of biosensors,the biomineralized microbial surface display technology was proposed.This review summarized the different microbial surface display systems and the biomineralized surface display systems,where the mechanisms of surface display and biomineralization were introduced.Then we described the recent progress of their applications on biosensors for different types of detection targets.Finally,the outlooks and tendencies were discussed and forecasted with the expectation to provide some general functions and enlightenments to this aspect of research.
文摘Biosurfactants are biologically active metabolites, and the efficiency of direct screening of new biosurfactants from nature using traditional methods is low, which should be enhanced in the following studies by adopting advanced biotechnologies. Rapid development and wide application of microbial culture independent methods, such as metagenomics, metatranscriptomics, metaproteomics and metabonomics, etc., contributes to quickly and precisely screening of novel biological surfactants. We mainly represented the current status of research and applications of biosurfactants in the remediation of petrochemical polluted environment, and also prospected avenues for future research.
基金supported by the Natural Science Foundation of China(32360029,32260003)Gui Da Tegang He Zi(2022)57,“Hundred”Talent Projects of Guizhou Province(Qian Ke He[2020]6005).
文摘Synthetic microbial community has widely concerned in the fields of agriculture,food and environment over the past few years.However,there is little consensus on the method to synthetic microbial community from construction to functional verification.Here,we review the concept,characteristics,history and applications of synthetic microbial community,summarizing several methods for synthetic microbial community construction,such as isolation culture,core microbiome mining,automated design,and gene editing.In addition,we also systematically summarized the design concepts,technological thresholds,and applicable scenarios of various construction methods,and highlighted their advantages and limitations.Ultimately,this review provides four efficient,detailed,easy-to-understand and-follow steps for synthetic microbial community construction,with major implications for agricultural practices,food production,and environmental governance.
基金supported by the Noncommunicable Chronic Diseases-National Science and Technology Major Project(2023ZD0500200)the RGC Collaborative Research Fund(C4008-23W)+3 种基金RGC Research Impact Fund Hong Kong(R4032-21F)Strategic Seed Funding Collaboration Research Scheme CUHK(3133344)Strategic Impact Enhancement Fund CUHK(3135509)Impact Case for RAE CUHK(3134277).
文摘Gastric cancer(GC)is one of the most common cancers worldwide particularly in Asian populations,and certain diets have been associated with increased risk of GC.Recent advances in microbial profiling technology have facilitated investigations on microbes residing on the gastric mucosa and increasing evidence has revealed the critical roles of non-Helicobacter pylori gastric microbes in gastric tumorigenesis.On the other hand,diets can affect microbial communities,causing compositional and functional shift of the microbiota.In this review,we summarize the influence of various diets including processed meat,salt-preserved food,high-fat diet,and alcohol on the development and progression of GC.We also explore microbial metabolites and host-microbe interactions in gastric tumorigenesis,alongside dietary interventions targeting the microbiota for the prevention and management against GC.
基金supported by the Spanish Ministry of Science,Innovation and Universities research project mobiMET.Ref:CPP2021-008936.M.A.G.and M.L.M.thank the Ministry of Science and Innovation(research project PID2022-140512NB-I00)。
文摘50%of pharmaceuticals and 25%of herbicides used worldwide are chiral.Each enantiomer has a unique toxicity and biodegradation profile,affecting differently to organisms.Chirality plays a key role in the behavior of these emerging contaminants(ECs)in terms of their pharmacological or herbicidal activity,but this peculiarity is often overlooked in environmental research.The complexity of chiral ECs is underestimated,as the varying sensitivity of biological systems to enantiomers is rarely considered.Biofilters can promote the activity of specific microbial communities,facilitating the degradation of ECs,due to the greater interaction between water and microorganisms and their compact design.Here,we show that an electroactive biofilter can alter the chirality of drugs and herbicides in wastewater treatment,impacting their removal and toxicity.The electrochemical biofilter(BioeF)removed 80%of pharmaceuticals and 50e75%of herbicides,outperforming the conventional filter(ConF).BioeF also showed greater chiral alterations and lower ecotoxicity.This work provides the first evidence of a relationship between changes in contaminant chirality and detoxification capacity,enhanced by electroactive systems.The increased microbial activity observed in the BioeF suggests that bioelectrochemical systems offer a valuable advance for ECs removal and ecotoxicity reduction,addressing the environmental challenge posed by ECs.
基金funded through the European Union's Horizon 2020 project ELECTRA[no.826244]National Nature Science Foundation of China(grant no.31861133002)+1 种基金S.P is a Serra Húnter Fellow(UdGAG-575)acknowledges the funding from the ICREA Academia award.LEQUiA[2021-SGR-01352]and Ecoaqua[2021-SGR-01142]have been recognized as consolidated research groups by the Catalan Government.
文摘It has been recently suggested that Alcaligenes use a previously unknown pathway to convert ammonium into dinitrogen gas(Dirammox)via hydroxylamine(NH2OH).This fact alone already implies a significant decrease in the aeration requirements for the process,but the process would still be dependent on external aeration.This work studied the potential use of a polarised electrode as an electron acceptor for ammonium oxidation using the recently described Alcaligenes strain HO-1 as a model heterotrophic nitrifier.Results indicated that Alcaligenes strain HO-1 requires aeration for metabolism,a requirement that cannot be replaced for a polarised electrode alone.However,concomitant elimination of succinate and ammonium was observed when operating a previously grown Alcaligenes strain HO-1 culture in the presence of a polarised electrode and without aeration.The usage of a polarised electrode together with aeration did not increase the succinate nor the nitrogen removal rates observed with aeration alone.However,current density generation was observed along a feeding batch test representing an electron share of 3%of the ammonium removed in the presence of aeration and 16%without aeration.Additional tests suggested that hydroxylamine oxidation to dinitrogen gas could have a relevant role in the electron discharge onto the anode.Therefore,the presence of a polarised electrode supported the metabolic functions of Alcaligenes strain HO-1 on the simultaneous oxidation of succinate and ammonium.
基金financed by the German Federal Ministry of Education and Research(BMBF)under the ElektroPapier project(Grant nr:03XP0041G)supported by the Helmholtz-Association within the Research Programme Renewable Energies.
文摘A laminar flow bioelectrochemical systems(BES)was designed and benchmarked using microbial anodes dominated with Geobacter spp.The reactor architecture was based on modeled flow fields,the resulting structure was 3D printed and used for BES manufacturing.Stratification of the substrate availability within the reactor channels led to heterogeneous biomass distribution,with the maximum biomass found mainly in the initial/middle channels.The anode performance was assessed for different hydraulic retention times while coulombic efficiencies of up to 100%(including also hydrogen recycling from the cathode)and current densities of up to 75 μA cm^(-2) at an anode surface to volume ratio of 1770 cm^(2) L^(-1) after 35 days were achieved.This low current density can be clearly attributed to the heterogeneous distributions of biomass and the stratification of the microbial community structure.Further,it was shown that time and space resolved analysis of the reactor microbiomes per channel is feasible using flow cytometry.
基金Acknowledgements This research was financially supported by Natural Science Foundation of Tianjin （Nos. 15JCYBJC53700 and 14JCYBJC- 43700）, National Undergraduate Training Programs for Innovation and Entrepreneurship （No. 201610057005）, Scientific Research Foundation for the Returned Overseas Chinese Scholars of State Education Ministry.
文摘A novel microorganism embedding material was prepared to enhance the biological nitrogen removal through simultaneous nitrification and denitrification. Polyvinyl alcohol （PVA）, sodium alginate （SA） and cyclodextrin （CD） were used to compose gel bead with embedded activated sludge. The effects of temperature, CD addition and concentrations of PYA and SA on nitrogen removal were evaluated. Results show that the gel bead with CD addition at 30℃contributed to the highest nitrogen removal efficiency and nitrogen removal rate of 85.4% and 2.08 mg·（L·h）^-1, respectively. Meanwhile, negligible NO3^- and NO2^- were observed, proving the occurrence of simultaneous nitrification and denitrification. The High-Throughput Sequencing confirms that the microbial community mainly contained Comamonadaceae in the proportion of 61.3%. Overall, CD increased gel bead＇s porosity and resulted in the high specific endogenous respiration rate and high nitrogen removal efficiency, which is a favorable additional agent to the traditional embedding material.
