Mining activities have caused significant land degradation globally,emphasizing the need for effective restoration.Microbial inoculants offer a promising solution for sustainable remediation by enhancing soil nutrient...Mining activities have caused significant land degradation globally,emphasizing the need for effective restoration.Microbial inoculants offer a promising solution for sustainable remediation by enhancing soil nutrients,enzyme activities,and microbial communities to support plant growth.However,the mechanisms by which inoculants influence soil microbes and their relationship with plant growth require further investigation.Metagenomic sequencing was employed for this study,based on a one-year greenhouse experiment,to elucidate the effects of Bacillus thuringiensis NL-11 on the microbial functions of abandoned mine soils.Our findings revealed that the application of microbial inoculants significantly enhanced the soil total carbon(TC),total sulfur(TS),organic carbon(SOC),available phosphorus(AP),ammonium(NH4+),urease,arylsulfatase,phosphatase,β-1,4-glucosidase(BG),β-1,4-N-acetylglucosaminidase(NAG).Moreover,this led to substantial improvements in plant height,as well as aboveground and belowground biomass.Microbial inoculants impacted functional gene structures without altering diversity.The normalized abundance of genes related to the degradation of carbon and nitrogen,methane metabolism,and nitrogen fixation were observed to increase,as well as the functional genes related to phosphorus cycling.Significant correlations were found between nutrient cycling gene abundance and plant biomass.Partial Least Squares Path Model analysis showed that microbial inoculants not only directly influenced plant biomass but also indirectly affected the plant biomass through C cycle modifications.This study highlights the role of microbial inoculants in promoting plant growth and soil restoration by improving soil properties and enhancing normalized abundance of nutrient cycling gene,making them essential for the recovery of abandoned mine sites.展开更多
Peanuts are important oilseed legume crops that are susceptible to contamination by Aspergillus flavus in soil,leading to serious economic losses.Previously,our research team developed the Aspergillus-Rihizobia coupli...Peanuts are important oilseed legume crops that are susceptible to contamination by Aspergillus flavus in soil,leading to serious economic losses.Previously,our research team developed the Aspergillus-Rihizobia coupling(ARC)microbial inoculants and found it can reduce A.flavus abundance in the soil and promote efficient nodulation in peanuts.However,the impact of ARC microbial inoculants on different resistant varieties of A.flavus remains unclear.In this study,we screened peanut varieties that were resistant and susceptible to A.flavus and evaluated their nodulation ability and growth performance after ARC microbial inoculants treatment in the field.The results demonstrated that the nodule number and nitrogenase activity of both varieties significantly increased after ARC microbial inoculants treatment,with the highly susceptible variety AH24 showing a greater increase.For photosynthetic parameters,both varieties also increased after ARC microbial inoculants treatment,but the increase was greater in the moderately resistant variety AH1 than in the highly susceptible variety AH24.Finally,we found that the yield and yield-related traits of the moderately resistant variety AH1 were better than those of the highly susceptible variety AH24.After ARC microbial inoculants treatment,the yield traits of both peanut varieties still increased significantly,but the degree of increase of the moderately resistant variety AH1 was smaller than that of the highly susceptible variety AH24.In addition,the abundance of A.flavus in the rhizosphere soil of the two varieties significantly decreased after ARC microbial inoculants treatment,with no significant difference between the varieties.These results indicated that ARC microbial inoculants exert differential effects on the nodulation and growth of different resistant peanut varieties and have a better effect on highly susceptible varieties.These results provide a solid theoretical basis for the efficient use of ARC microbial inoculants in the field of peanuts in the future.展开更多
A microbial inoculant, known as effective microorganisms （EM）, was applied to determine its efficacy on seed germination and seedling growth in the nursery of Acacia auriculiformis A Curm. ex Benth. The seedlings we...A microbial inoculant, known as effective microorganisms （EM）, was applied to determine its efficacy on seed germination and seedling growth in the nursery of Acacia auriculiformis A Curm. ex Benth. The seedlings were grown in a mixture of sandy soil and cow dung （3：1） and kept in polybags; EM was poured at different concentra- tions （0.1%, 0.5%, 1%, 2%, 5% and 10%）. Seed germination rate and growth parameters of seedlings - shoot and root lengths, fresh and dry weights of shoots and roots, vigor, volume, and quality indices and stur- diness - were measured. The nodulation status influenced by EM was also observed, along with the measurement ofpigrnent contents in leaves. The highest germination rate （72%） was observed in 2% EM solution while the lowest （55%） was found in control treatment. The highest shoot and root lengths （30.6 cm and 31.2 cm respectively） were recorded in 2% EM and were significantly （p 〈0.05） different from control. Both fresh and dry weights of shoots were maximum （8.66 g and 2.99 g respectively） in 2% EM, whereas both fresh and dry weights of root were maximum （2.56 g and 1.23 g respectively） in 5% EM solution. Although the highest vigor index, volume index, and sturdiness （4450, 628 and 67.5 respec- tively） were found in 2% EM, the highest quality index （0.455） was found in 5% EM solution. The nodule number was higher at a very low （0.5%） concentration of EM but it normally decreased with the increase of concentration. The contents of chlorophyll a, chlorophyll b, and carotenoid were maximum （43.26 mg.L-1, 13.56 mg.L-1 and 17.99 mg.L-1 respectively） in 2% EM. Therefore, low concentration of EM （up to 2%）can be recommended for getting maximum seed germination and seed- ling development ofA. auriculiformis in the nursery.展开更多
Under field conditions, an experiment was conducted to study the effects of ammonification bacteria, potassium bacteria and phosphorus bacteria on nutrient availability in soil and yield of rice in the cold region of ...Under field conditions, an experiment was conducted to study the effects of ammonification bacteria, potassium bacteria and phosphorus bacteria on nutrient availability in soil and yield of rice in the cold region of China and compared to the conventional fertilization. Results showed that DF1P2 treatment （ammonifiers 1.5× 108 cfu· m2, phosphorus bacteria 1.5× 108 cfu. m2, and potassium bacteria 1.5× 108 cfu· m2） increased available nutrient concentrations in soil, increased the concentrations of N, P, and K in plant organs and increased the rice yield and was the most significantly among all the treatments. This treatment could be recommended as the best suitable biological fertilizer application rate for the rice production in the cold region of China.展开更多
Whole crop forage sorghum (Saccharatum) cultivar FS5 was harvested at the soft dough stage of maturity. The sorghum was chopped to approximately 2 cm pieces and ensiled under laboratory conditions in 1.5 L Weck glass ...Whole crop forage sorghum (Saccharatum) cultivar FS5 was harvested at the soft dough stage of maturity. The sorghum was chopped to approximately 2 cm pieces and ensiled under laboratory conditions in 1.5 L Weck glass jars. At ensiling, it was treated with two commercial microbial inoculants: inoculant A and inoculant B. The inoculants were applied at 2 × 105 colony forming units g-1 DM. Silage with no additives served as a control. Three jars per treatment were opened on days 2, 4, 8, 15 and 60 post-ensiling to study fermentation dynamics. After 60 days of ensiling, the silages were analyzed and subjected to an aerobic stability test lasting 5 days. Results showed that both inoculants caused a more rapid rate of pH decline and a higher amount of lactic acid production. Silages treated with each inoculant produced a little more CO2 and resulted in more glucose loss as compared with the control. Addition of inoculants did not influence (P > 0.05) the ash and crude protein contents, but tended to decrease the concentration of acetic acid (P < 0.05), butyric acid (P<0.01) and propionic acid (P<0.01), and increase the lactic acid concentration (P<0.01). Silages treated with inoculant A possess the more DM loss, and the higher yeast counts upon aerobic exposure. Silage treated with inoculant B had the most DM (P<0.05), lactic acid contents (P<0.01), the least acetic acid content (P<0.05). Inoculant B reduced the ADF (P<0.01), ADL and NDF (P<0.05) contents. It was concluded that lactic bacteria inoculants may improve the fermentation but might impair the aerobic stability for sorghum ensilage.展开更多
Nursery practice using microbial inoculants was performed to find out the efficacy of the inoculants on seed germination and seedling growth of sissoo（Dalbergia sissoo Roxb.）.Microbial inoculants or effective microo...Nursery practice using microbial inoculants was performed to find out the efficacy of the inoculants on seed germination and seedling growth of sissoo（Dalbergia sissoo Roxb.）.Microbial inoculants or effective microorganisms（EM） are a mixture of many different beneficial microorganisms in a solution.The seedlings were grown in a mixture of sandy soil and cowdung（3：1） kept in polybags with pouring EM solution at different concentrations（0.1%,0.5%,1%,2%,5% and 10%） before and after a week of sowing the seeds.Seed germination rate and growth parameters of seedlings were measured,such as,shoot and root length,vigor index,fresh and dry weight of shoot and root and total biomass increment.The nodulation status influenced by EM was also observed along with the measurement of pigment contents in leaves.The highest germination rate（69%） was observed in 2% EM treatment,followed by 67% and 65% in 1% and 5% EM.The highest shoot length（33.2 cm） was in 2% EM,whereas highest root length（26.3 cm） was in 1% EM.Both fresh and dry weights from shoot and root,were maximum（4.16 g and 1.57 g;2.12 g and 0.83 g respectively） in 2% EM and were significantly（p ≤ 0.05） different from control.Vigor index was highest（4071） in 2% EM,which was significantly（p ≤ 0.05） different from control.Total dry weight increment was highest in 2% EM treatment,followed by 1% and 5% concentrations of EM.Nodulation number was higher at very low（0.1%） concentration of EM solution but it normally decreased with the increase of EM concentrations.The contents of chlorophyll a,chlorophyll b and carotenoid were highest（60.11,17.05 and 42.48 mg·L1respectively） in 2% EM treatment and lowest（39.35,13.55 and 27.29 mg·L-1 respectively） in control treatment.Therefore,low concentration of EM（up to 2%） can be used for getting maximum seed germination rate and seedling development of Dalbergia sissoo Roxb.展开更多
Microbial Inoculants as Effective Microorganisms （EM） were applied to find out their effects on germination and seedling growth of Albizia saman in the nursery. The seedlings were grown in a mixture of sandy soils a...Microbial Inoculants as Effective Microorganisms （EM） were applied to find out their effects on germination and seedling growth of Albizia saman in the nursery. The seedlings were grown in a mixture of sandy soils and cow dung （3：1） kept in polybags. The EM solution at different concentrations （0.1%, 0.5%, 1%, 2%, 5% and 10%） was incorporated before and after a week of sowing seeds. Germination and physical growth parameters, including shoot and root length, vigor index, collar diameter, leaf number, fresh and dry weight of shoot and root and total biomass increment over the control were measured. The nodulation status influenced by EM was also observed along with the estimation of chemical parameters like chlorophyll a, chlorophyll b and carotenoid. Both germination and the measured physical growth parameters were found significantly （P〈0.05） higher in seedlings treated with different concentrations of EM solution in comparison to the control. Maximum growth was found at 2% followed by 1% EM solution. Nodulation was higher at 0.1% concentration but it normally decreased with the increase of concentrations. Although there were a higher amount of pigments in leaves of the treated seedlings than of the control, the variations recorded with respect to chlorophyll a, b and carotenoid were not significantly higher in most of the treatments. Treated seedlings showed variable results along with the increment of EM applications and most of the parameters showed best results at the medium range of concentrations. The study indicates that the Microbial Inoculant （EM） technology might be useful to improve the growth of seedlings in the nursery. This also indicates that the associated beneficial organisms along with the polybag soils might be of value in improving the degraded soil or poor field soil for better nutrient and water uptake during the initial growth of transplanted seedlings.展开更多
[Objective] The aim was to study the effects of compound microbial inoculants on fermentation bed.[Method] With fermentation simulated in lab,analysis was conducted on changes of temperature,pH,nitrate nitrogen,ammoni...[Objective] The aim was to study the effects of compound microbial inoculants on fermentation bed.[Method] With fermentation simulated in lab,analysis was conducted on changes of temperature,pH,nitrate nitrogen,ammonium nitrogen,urease activity and protease activity in fermentation by microorganism and natural fermentation respectively,to explore effects of compound microbial inoculants on fermentation bed of swine.[Result] Compared with control group,the added microbial inoculants in test group promoted temperature rising during fermentation and prolonged lasting period of high temperature,for example,high temperature at 60 ℃ maintained 10 d in the test.Furthermore,the inoculants reduced pH of packaging material environment,for example,pH finally was 7.05,lower than that of control group.Microbial inoculants accelerated transformation of ammonium nitrogen into nitrate nitrogen and reduced nitrogen loss.In addition,activities of urease and protease enhanced in packaging materials and excrements degraded rapidly.[Conclusion] The research provides technical references for strain development,selection and evaluation of fermentation bed of swine.展开更多
Beneficial microorganisms were extracted from municipal solid waste compost and treated under low temperature stress to prepare complex microbial inoc- ulants. Turfgrass was inoculated with the prepared microbial inoc...Beneficial microorganisms were extracted from municipal solid waste compost and treated under low temperature stress to prepare complex microbial inoc- ulants. Turfgrass was inoculated with the prepared microbial inoculants to investigate the ecological effect of ~crobial inoculants on turf storage in winter. The re- suits showed that complex low temperature tolerant microbial inoculants significandy improved plant height and aboveground biomass of turfgrass. Compared with the non-inoculated control, plant height and biomass of the first batch of inoculated turfgrass were enhanced by 6.47% and 14.32%, respectively; and they were en- hanced by 6.94% and 17.41%, respectively, for the second batch of inoculated turfgrass. Under low temperature stress in winter, the reviving rate of inoculated tarfgrass was significandy higher than that of control, which was improved by 3.34% and 43.33% for the first and second batches of inoculated turfgrass respective- ly. After low temperature storage in winter, inoculated turfgrass revived in advance; specifically, the reviving date of the second batch of inoculated turfgrass was 3 d earlier than that of control. This low temperature tolerant microbial consortium was effective for turfgrass as an ecofriendly and acceptable technology to improve plant performance and development in winter and accelerate time to market of turf.展开更多
Microbial inoculants were isolated from sewage sludge and applied to turfgrass medium. Effects of microbial inoculants on seed germination and initial growth of Festuca arundinacea L. were analyzed to investigate the ...Microbial inoculants were isolated from sewage sludge and applied to turfgrass medium. Effects of microbial inoculants on seed germination and initial growth of Festuca arundinacea L. were analyzed to investigate the optimal proportion of compound microbial inoculants. Significant differences were found in various growth indices and chlorophyll content of F. arundinacea between microbial treatment and control The combination of Pseudomonas stutzeri and Trichoderma reesei exhibited the best results. Compared with the control, seed germination rate, seedling height, shoot dry weight, root weight, chlorophyll a content and chlorophyll b content of F. arundinacea treated with the combination of Pseudomonas stutzeri and Trichoderma reesei were improved by 11.60%, 43.47%, 148%, 159%, 144% and 136%, respectively. Based on comprehensive analysis of various indicators, the optimal proportion （V/V） of microbial combination was Pseudomonas stutzeri： Tfichoderma reesei = 1： 1, which was the most conducive to plant growth and development.展开更多
Microbial inoculants have received increasing attention in strengthening plant biological barriers,antagonizing and inhibiting harmful microorganisms,and ensuring the safe production of agricultural products.This pape...Microbial inoculants have received increasing attention in strengthening plant biological barriers,antagonizing and inhibiting harmful microorganisms,and ensuring the safe production of agricultural products.This paper summarized the research status of agricultural microbial inoculants,the application of microbial inoculants in agriculture,and the trends and prospects of agricultural microbial research.展开更多
Wastewater treated by compound microbial inoculant(CMI)in agricultural irrigation can enrich soil fertility and decompose the possible pollutants.In this study,a greenhouse experiment using tomato as the model crop wa...Wastewater treated by compound microbial inoculant(CMI)in agricultural irrigation can enrich soil fertility and decompose the possible pollutants.In this study,a greenhouse experiment using tomato as the model crop was performed to investigate the effects of treated wastewater irrigation on soil nutrients and enzymes.For this purpose,certain parameters were measured,including soil total nitrogen(N),nitrate N,total phosphorus(P),available potassium(K)and the activities of the enzymes urease,acid phosphatase and catalase in soils irrigated with fresh water,wastewater and CMI-treated wastewater under three amount of irrigation water.The results showed that irrigation with both treated and untreated wastewater significantly increased soil total N,total P,and available K,however the treated wastewater showed higher effects on soil enrichment,especially on available K.The activity of soil urease and acid phosphatase reached highest with treated wastewater irrigation,whereas wastewater irrigation increased the activity of catalase obviously.Soil enzyme and nutrient with fresh water irrigation decreased with increasing water amount;the content of soil urease,nitrate-nitrogen,total N and total P in treated wastewater and wastewater irrigation rose with increasing water amount,but the highest activity of acid phosphatase and the lowest activity of catalase were found in medium irrigation water amount.Under the condition of tomato cultivation,total N,nitrate N and total P were closely correlated with soil urease and catalase;there were significant positive correlation among soil urease,catalase,total N,nitrate N and total P;there existed significantly positive correlation between acid phosphatase and all measured soil nutrient indexes.The results suggested that irrigation with CMI-treated wastewater is a security and effective strategy to agricultural land management.展开更多
Objective:To find a suitable ecological cultivation measure to solve the problem of root-knot nematode disease of Panax quinquefolium(Panacis Quinquefolii Radix)and the heavy metals accumulating in its roots.Methods:T...Objective:To find a suitable ecological cultivation measure to solve the problem of root-knot nematode disease of Panax quinquefolium(Panacis Quinquefolii Radix)and the heavy metals accumulating in its roots.Methods:Three-year-old P.quinquefolium was treated with four different combinations of microbial inoculant(MI)and garbage fermentation liquid(GFL)[the joint application of‘TuXiu’MI and Fifty potassium MI(TF),the combination use of‘No.1'MI and Fifty potassium MI(NF),‘Gulefeng’poly-γ-glutamic acid MI(PGA),GFL],and the untreated control(CK).Here,high-throughput sequencing,ICP-MS and UPLC were employed to systematically characterize changes of microbial diversity and structure composition,heavy metals(As,Cd and Pb)content and ginsenoside content among different treatments.Results:The results revealed that different MIs and GFL could increase the root dry weight of P.quinquefolium,PGA enhanced it by 83.24%,followed by GFL(49.93%),meanwhile,PGA and GFL were able to lessen root-knot nematode disease incidence by 57.25%and 64.35%.The treatment of PGA and GFL can also effectively reduce heavy metals in roots.The As content in GFL and PGA was decreased by 52.17%and 43.48%respectively,while the Cd and Pb contents of GFL and PGA was decreased somewhat.Additionally,the content of total ginsenosides was increased by 42.14%and 42.07%,in response to TF and NF,respectively.Our metagenomic analysis showed that the relative abundance of particular soil microbial community members related to the biocontrol of root-knot nematode disease and plant pathogen(i.e.,Chaetomium in NF,Xylari in GFL,and Microascus in PGA),heavy metal bioremediation(Hyphomacrobium in PGA and Xylaria in GFL),and nitrogen fixation(Nordella and Nitrospira in TF)was significantly increased;notably,potential harmful microflora,such as Plectosaphaerella and Rhizobacter,were more abundant in the control group.Conclusion:MI and GFL could improve the quality of P.quinquefolium by modifying its rhizosphere microbial community structure and composition,both of them are beneficial to the development of ecological cultivation of P.quinquefolium.展开更多
Although the application of straw decomposing microorganism inoculants(SDMI)can accelerate straw decomposition,the underlying mechanisms affecting soil organic carbon(SOC)under different scenarios remain unclear.We co...Although the application of straw decomposing microorganism inoculants(SDMI)can accelerate straw decomposition,the underlying mechanisms affecting soil organic carbon(SOC)under different scenarios remain unclear.We conducted a meta-analysis using 226 observations from 86 studies on SOC changes under straw return with or without SDMI applications.Overall,our results indicated that straw with SDMI application increased the SOC stock by 1.51%at an initial carbon-to-nitrogen ratio(ICNR)>25(P<0.05),while the effect of ICNR≤25was insignificant.In particular,at ICNR>25,application of SDMI-treated straw increased SOC stocks in northern temperate continental areas(NTC)higher than in subtropical monsoon regions(STM).Furthermore,the straw with SDMI application increased higher SOC stocks in soils with pH>7.5 than those with pH≤7.5.In terms of agricultural management practices,SOC stocks were significantly higher in straw buried(SB),the experimental duration of straw return(EDSR)≥1 year,the straw return amount(SRA)>6,000 kg ha^(–1),and the SDMI application rate(SDMIR)>30 kg ha^(–1)conditions.The effect of straw with SDMI on SOC stocks under straw burying(SB)was significantly higher than that under straw mulching(SM)at ICNR≤25.At ICNR>25,EDSR,SDMIR,and the mean annual precipitation(MAP)were the main drivers of the effect of SDMI addition to straw on SOC stocks.Straw with SDMI induced SOC stock increases which increased with EDSR and decreased with increasing MAP.These findings provide a scientific basis for decision-makers and stakeholders to improve soil C management via the application of SDMI-amended straw at both regional and large scales.展开更多
Agricultural areas of land are deteriorating every day owing to population increase, rapid urbanization, and industrialization. To feed today’s huge populations, increased crop production is required from smaller are...Agricultural areas of land are deteriorating every day owing to population increase, rapid urbanization, and industrialization. To feed today’s huge populations, increased crop production is required from smaller areas, which warrants the continuous application of high doses of inorganic fertilizers to agricultural land. These cause damage to soil health and, therefore, nutrient imbalance conditions in arable soils. Under these conditions, the benefits of microbial inoculants (such as Actinobacteria) as replacements for harmful chemicals and promoting ecofriendly sustainable farming practices have been made clear through recent technological advances. There are multifunctional traits involved in the production of different types of bioactive compounds responsible for plant growth promotion, and the biocontrol of phytopathogens has reduced the use of chemical fertilizers and pesticides. There are some well-known groups of nitrogen-fixing Actinobacteria, such as Frankia, which undergo mutualism with plants and offer enhanced symbiotic trade-offs.In addition to nitrogen fixation, increasing availability of major plant nutrients in soil due to the solubilization of immobilized forms of phosphorus and potassium compounds, production of phytohormones, such as indole-3-acetic acid, indole-3-pyruvic acid, gibberellins, and cytokinins, improving organic matter decomposition by releasing cellulases, xylanase, glucanases, lipases, and proteases, and suppression of soil-borne pathogens by the production of siderophores, ammonia, hydrogen cyanide, and chitinase are important features of Actinobacteria useful for combating biotic and abiotic stresses in plants.The positive influence of Actinobacteria on soil fertility and plant health has motivated us to compile this review of important findings associated with sustaining plant productivity in the long run.展开更多
On site composting of organic household wastes is an economical and environmentally tiiendly way to manage municipal wastes. In this manuscript authors evaluated the importance of turning the wastes and of inoculating...On site composting of organic household wastes is an economical and environmentally tiiendly way to manage municipal wastes. In this manuscript authors evaluated the importance of turning the wastes and of inoculating microorganisms and worms in order to unprove the composting process at domestic scale. Four treatments （Control without turning- C-, Control with Turning- CT- inoculated Mountain Microorganism with turning- MM- and Worms without turning- W-） were tested in a random experiment with tour replicates. Sixteen composting bins were fed with 300 kg of organic wastes from a local street market. The process of compostnig lasted 13 weeks after which all the composts were sifted and submitted to a range of chemical, physical and biological analysis. According to the results MM slightly increased the initial temperature and enzymatic activiry. This investigation outcome don＇t provide of sufficient grounds for a precise recommendation about worms inoculation in compost bins. A decrease between 75% and 80% in the fresh weight of the organic waste was found in the first thirteen weeks after starting the composting process in all the treatments. The quality level of the compost was acceptable, with very low heavy metal content. Turn over helps to keep the product hygienic especially after the inoculation with MM microbial starts. In conclusion and according to the results of this investigation, the use of 320 L compost bins for the organic waste management is strongly advisable.展开更多
Recently,returning straw to the fields has been proved as a direct and effective method to tackle soil nutrient loss and agricultural pollution.Meanwhile,the slow decomposition of straw may harm the growth of the next...Recently,returning straw to the fields has been proved as a direct and effective method to tackle soil nutrient loss and agricultural pollution.Meanwhile,the slow decomposition of straw may harm the growth of the next crop.This study aimed to determine the effects of rumen microorganisms(RMs)on straw decomposition,bacterial microbial community structure,soil properties,and soil enzyme activity.The results showed that RMs significantly enhanced the degradation rate of straw in the soil,reaching 39.52%,which was 41.37%higher than that of the control on the 30th day after straw return.After 30 d,straw degradation showed a significant slower trend in both the control and the experimental groups.According to the soil physicochemical parameters,the application of rumen fluid expedited soil matter transformation and nutrient buildup,and increased the urease,sucrase,and cellulase activity by 10%‒20%.The qualitative analysis of straw showed that the hydroxyl functional group structure of cellulose in straw was greatly damaged after the application of rumen fluid.The analysis of soil microbial community structure revealed that the addition of rumen fluid led to the proliferation of Actinobacteria with strong cellulose degradation ability,which was the main reason for the accelerated straw decomposition.Our study highlights that returning rice straw to the fields with rumen fluid inoculation can be used as an effective measure to enhance the biological value of recycled rice straw,proposing a viable solution to the problem of sluggish straw decomposition.展开更多
A field experiment was carried out in Cd-contaminated rice fields in a county of Hengyang to explore the effects of different types of remediation agents on the contents of soil available Cd and rice Cd,and rice yield...A field experiment was carried out in Cd-contaminated rice fields in a county of Hengyang to explore the effects of different types of remediation agents on the contents of soil available Cd and rice Cd,and rice yield by using one rice variety Longliangyouhuazhan,and 4 kinds of remediation agents:DG foliar control agent,SMA microbial inoculant,XFJ organic fertilizer and LXM calcium-and silicon-based passivator as test materials.The results showed that compared with CK,after applying DG foliar control agent,soil available Cd content increased rather than decreased,whereas rice Cd content decreased,not reaching a significant level.When applied with SMA microbial inoculant,soil pH value,soil available Cd and rice Cd contents showed a downward then upward trend with the increase of its application rates.Between 3 levels of SMA treatments,the content of soil available Cd in SMA2 was the lowest,decreased by 8.59%in comparison with CK,contrarily,two other SMA treatments were increasing instead of decreasing in the content of soil available Cd.The application of XFJ organic fertilizer and LXM calcium-and silicon-based passivator increased soil pH value,and reduced soil available Cd and rice Cd contents;the contents of soil available Cd and rice Cd decreased with their application rates increasing.Compared with CK,XFJ3 reduced soil available Cd content by 9.40%,and significantly reduced rice Cd content by 57.28%.In LXM3 treatment,soil available Cd content reduced by 14.57%,rice Cd content was 71.57%lower than CK,reaching the lowest level.In general,LXM calcium-and silicon-based passivator had the best Cd reduction effect,with the optimal application amount of 6000 kg/hm^(2).展开更多
The aim of this work was to see whether Pseudomonas putida NWU12, Pseudomonas fluorescence NWU65, Vibrio fluvialis NWU37 and Ewingella americana NWU59 are beneficial to plants and are able to promote plant growth and ...The aim of this work was to see whether Pseudomonas putida NWU12, Pseudomonas fluorescence NWU65, Vibrio fluvialis NWU37 and Ewingella americana NWU59 are beneficial to plants and are able to promote plant growth and development when inoculated as plant growth-promoting rhizobacteria (PGPR). The four rhizobacteria were tested in vitro for PGPR activities and on spinach and pepper in pot experiments. The inoculants are all positive for ammonia (NH3 ), catalase, hydrogen cyanide (HCN), phosphate solubilization and siderophore production. Among the inoculants, E. americana NWU59 is oxidase negative. P. putida NWU12 and P. fluorescence NWU65 are producing indole-3-acetic acid (IAA). The inoculants exhibit some PGPR activities and thus tested in the screen-house. Treatments are control (water) and the four inoculants. Rhizobacterial inoculants increase spinach (17.14%-21.43%) and pepper (15.0%-37.5%) plant heights over the control. Such inoculants have the potential of improving plant yield components and may be used as biofertilizer.展开更多
This work is devoted to developing of the ecologically safe technology of chickpea cultivation by using microbial biopreparations, based on the effective heterotrophic （nodulating, growth-promoting and phosphate mobi...This work is devoted to developing of the ecologically safe technology of chickpea cultivation by using microbial biopreparations, based on the effective heterotrophic （nodulating, growth-promoting and phosphate mobilizing bacteria, and microorganisms-antagonists of the phytopathogenes） and phototrophic microorganisms （cyanobacteria）. These preparations were used for pre-sowing treatment of seeds and for treatment of the plants in the vegetation period for optimization of symbiotic nitrogen fixation and growing of ecologically safe production （without application of mineral fertilizers） in the steppe non-irrigated areas of Ukraine.展开更多
基金supported by the Jiangsu Science and Technology Plan Project(No.BE2022420)the Innovation and Promotion of Forestry Science and Technology Program of Jiangsu Province(No.LYKJ[2021]30)+2 种基金the Scientific Research Project of Baishanzu National Park(No.2021ZDLY01)the Ningxia key research and development plan(No.2021BEG02010)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Mining activities have caused significant land degradation globally,emphasizing the need for effective restoration.Microbial inoculants offer a promising solution for sustainable remediation by enhancing soil nutrients,enzyme activities,and microbial communities to support plant growth.However,the mechanisms by which inoculants influence soil microbes and their relationship with plant growth require further investigation.Metagenomic sequencing was employed for this study,based on a one-year greenhouse experiment,to elucidate the effects of Bacillus thuringiensis NL-11 on the microbial functions of abandoned mine soils.Our findings revealed that the application of microbial inoculants significantly enhanced the soil total carbon(TC),total sulfur(TS),organic carbon(SOC),available phosphorus(AP),ammonium(NH4+),urease,arylsulfatase,phosphatase,β-1,4-glucosidase(BG),β-1,4-N-acetylglucosaminidase(NAG).Moreover,this led to substantial improvements in plant height,as well as aboveground and belowground biomass.Microbial inoculants impacted functional gene structures without altering diversity.The normalized abundance of genes related to the degradation of carbon and nitrogen,methane metabolism,and nitrogen fixation were observed to increase,as well as the functional genes related to phosphorus cycling.Significant correlations were found between nutrient cycling gene abundance and plant biomass.Partial Least Squares Path Model analysis showed that microbial inoculants not only directly influenced plant biomass but also indirectly affected the plant biomass through C cycle modifications.This study highlights the role of microbial inoculants in promoting plant growth and soil restoration by improving soil properties and enhancing normalized abundance of nutrient cycling gene,making them essential for the recovery of abandoned mine sites.
基金supported by the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202416)the Foundation of Hubei Hongshan Laboratory(2021hszd015)+1 种基金the Science and Technology Major Projects of Hubei Province(2023BBA002)the Knowledge Innovation Program of Wuhan-Basi Research(2023020201010126)。
文摘Peanuts are important oilseed legume crops that are susceptible to contamination by Aspergillus flavus in soil,leading to serious economic losses.Previously,our research team developed the Aspergillus-Rihizobia coupling(ARC)microbial inoculants and found it can reduce A.flavus abundance in the soil and promote efficient nodulation in peanuts.However,the impact of ARC microbial inoculants on different resistant varieties of A.flavus remains unclear.In this study,we screened peanut varieties that were resistant and susceptible to A.flavus and evaluated their nodulation ability and growth performance after ARC microbial inoculants treatment in the field.The results demonstrated that the nodule number and nitrogenase activity of both varieties significantly increased after ARC microbial inoculants treatment,with the highly susceptible variety AH24 showing a greater increase.For photosynthetic parameters,both varieties also increased after ARC microbial inoculants treatment,but the increase was greater in the moderately resistant variety AH1 than in the highly susceptible variety AH24.Finally,we found that the yield and yield-related traits of the moderately resistant variety AH1 were better than those of the highly susceptible variety AH24.After ARC microbial inoculants treatment,the yield traits of both peanut varieties still increased significantly,but the degree of increase of the moderately resistant variety AH1 was smaller than that of the highly susceptible variety AH24.In addition,the abundance of A.flavus in the rhizosphere soil of the two varieties significantly decreased after ARC microbial inoculants treatment,with no significant difference between the varieties.These results indicated that ARC microbial inoculants exert differential effects on the nodulation and growth of different resistant peanut varieties and have a better effect on highly susceptible varieties.These results provide a solid theoretical basis for the efficient use of ARC microbial inoculants in the field of peanuts in the future.
文摘A microbial inoculant, known as effective microorganisms （EM）, was applied to determine its efficacy on seed germination and seedling growth in the nursery of Acacia auriculiformis A Curm. ex Benth. The seedlings were grown in a mixture of sandy soil and cow dung （3：1） and kept in polybags; EM was poured at different concentra- tions （0.1%, 0.5%, 1%, 2%, 5% and 10%）. Seed germination rate and growth parameters of seedlings - shoot and root lengths, fresh and dry weights of shoots and roots, vigor, volume, and quality indices and stur- diness - were measured. The nodulation status influenced by EM was also observed, along with the measurement ofpigrnent contents in leaves. The highest germination rate （72%） was observed in 2% EM solution while the lowest （55%） was found in control treatment. The highest shoot and root lengths （30.6 cm and 31.2 cm respectively） were recorded in 2% EM and were significantly （p 〈0.05） different from control. Both fresh and dry weights of shoots were maximum （8.66 g and 2.99 g respectively） in 2% EM, whereas both fresh and dry weights of root were maximum （2.56 g and 1.23 g respectively） in 5% EM solution. Although the highest vigor index, volume index, and sturdiness （4450, 628 and 67.5 respec- tively） were found in 2% EM, the highest quality index （0.455） was found in 5% EM solution. The nodule number was higher at a very low （0.5%） concentration of EM but it normally decreased with the increase of concentration. The contents of chlorophyll a, chlorophyll b, and carotenoid were maximum （43.26 mg.L-1, 13.56 mg.L-1 and 17.99 mg.L-1 respectively） in 2% EM. Therefore, low concentration of EM （up to 2%）can be recommended for getting maximum seed germination and seed- ling development ofA. auriculiformis in the nursery.
文摘Under field conditions, an experiment was conducted to study the effects of ammonification bacteria, potassium bacteria and phosphorus bacteria on nutrient availability in soil and yield of rice in the cold region of China and compared to the conventional fertilization. Results showed that DF1P2 treatment （ammonifiers 1.5× 108 cfu· m2, phosphorus bacteria 1.5× 108 cfu. m2, and potassium bacteria 1.5× 108 cfu· m2） increased available nutrient concentrations in soil, increased the concentrations of N, P, and K in plant organs and increased the rice yield and was the most significantly among all the treatments. This treatment could be recommended as the best suitable biological fertilizer application rate for the rice production in the cold region of China.
文摘Whole crop forage sorghum (Saccharatum) cultivar FS5 was harvested at the soft dough stage of maturity. The sorghum was chopped to approximately 2 cm pieces and ensiled under laboratory conditions in 1.5 L Weck glass jars. At ensiling, it was treated with two commercial microbial inoculants: inoculant A and inoculant B. The inoculants were applied at 2 × 105 colony forming units g-1 DM. Silage with no additives served as a control. Three jars per treatment were opened on days 2, 4, 8, 15 and 60 post-ensiling to study fermentation dynamics. After 60 days of ensiling, the silages were analyzed and subjected to an aerobic stability test lasting 5 days. Results showed that both inoculants caused a more rapid rate of pH decline and a higher amount of lactic acid production. Silages treated with each inoculant produced a little more CO2 and resulted in more glucose loss as compared with the control. Addition of inoculants did not influence (P > 0.05) the ash and crude protein contents, but tended to decrease the concentration of acetic acid (P < 0.05), butyric acid (P<0.01) and propionic acid (P<0.01), and increase the lactic acid concentration (P<0.01). Silages treated with inoculant A possess the more DM loss, and the higher yeast counts upon aerobic exposure. Silage treated with inoculant B had the most DM (P<0.05), lactic acid contents (P<0.01), the least acetic acid content (P<0.05). Inoculant B reduced the ADF (P<0.01), ADL and NDF (P<0.05) contents. It was concluded that lactic bacteria inoculants may improve the fermentation but might impair the aerobic stability for sorghum ensilage.
文摘Nursery practice using microbial inoculants was performed to find out the efficacy of the inoculants on seed germination and seedling growth of sissoo（Dalbergia sissoo Roxb.）.Microbial inoculants or effective microorganisms（EM） are a mixture of many different beneficial microorganisms in a solution.The seedlings were grown in a mixture of sandy soil and cowdung（3：1） kept in polybags with pouring EM solution at different concentrations（0.1%,0.5%,1%,2%,5% and 10%） before and after a week of sowing the seeds.Seed germination rate and growth parameters of seedlings were measured,such as,shoot and root length,vigor index,fresh and dry weight of shoot and root and total biomass increment.The nodulation status influenced by EM was also observed along with the measurement of pigment contents in leaves.The highest germination rate（69%） was observed in 2% EM treatment,followed by 67% and 65% in 1% and 5% EM.The highest shoot length（33.2 cm） was in 2% EM,whereas highest root length（26.3 cm） was in 1% EM.Both fresh and dry weights from shoot and root,were maximum（4.16 g and 1.57 g;2.12 g and 0.83 g respectively） in 2% EM and were significantly（p ≤ 0.05） different from control.Vigor index was highest（4071） in 2% EM,which was significantly（p ≤ 0.05） different from control.Total dry weight increment was highest in 2% EM treatment,followed by 1% and 5% concentrations of EM.Nodulation number was higher at very low（0.1%） concentration of EM solution but it normally decreased with the increase of EM concentrations.The contents of chlorophyll a,chlorophyll b and carotenoid were highest（60.11,17.05 and 42.48 mg·L1respectively） in 2% EM treatment and lowest（39.35,13.55 and 27.29 mg·L-1 respectively） in control treatment.Therefore,low concentration of EM（up to 2%） can be used for getting maximum seed germination rate and seedling development of Dalbergia sissoo Roxb.
文摘Microbial Inoculants as Effective Microorganisms （EM） were applied to find out their effects on germination and seedling growth of Albizia saman in the nursery. The seedlings were grown in a mixture of sandy soils and cow dung （3：1） kept in polybags. The EM solution at different concentrations （0.1%, 0.5%, 1%, 2%, 5% and 10%） was incorporated before and after a week of sowing seeds. Germination and physical growth parameters, including shoot and root length, vigor index, collar diameter, leaf number, fresh and dry weight of shoot and root and total biomass increment over the control were measured. The nodulation status influenced by EM was also observed along with the estimation of chemical parameters like chlorophyll a, chlorophyll b and carotenoid. Both germination and the measured physical growth parameters were found significantly （P〈0.05） higher in seedlings treated with different concentrations of EM solution in comparison to the control. Maximum growth was found at 2% followed by 1% EM solution. Nodulation was higher at 0.1% concentration but it normally decreased with the increase of concentrations. Although there were a higher amount of pigments in leaves of the treated seedlings than of the control, the variations recorded with respect to chlorophyll a, b and carotenoid were not significantly higher in most of the treatments. Treated seedlings showed variable results along with the increment of EM applications and most of the parameters showed best results at the medium range of concentrations. The study indicates that the Microbial Inoculant （EM） technology might be useful to improve the growth of seedlings in the nursery. This also indicates that the associated beneficial organisms along with the polybag soils might be of value in improving the degraded soil or poor field soil for better nutrient and water uptake during the initial growth of transplanted seedlings.
基金Supported by Construction of Innovative Enterprises of Changsha Science and Technology Program(K1112050)~~
文摘[Objective] The aim was to study the effects of compound microbial inoculants on fermentation bed.[Method] With fermentation simulated in lab,analysis was conducted on changes of temperature,pH,nitrate nitrogen,ammonium nitrogen,urease activity and protease activity in fermentation by microorganism and natural fermentation respectively,to explore effects of compound microbial inoculants on fermentation bed of swine.[Result] Compared with control group,the added microbial inoculants in test group promoted temperature rising during fermentation and prolonged lasting period of high temperature,for example,high temperature at 60 ℃ maintained 10 d in the test.Furthermore,the inoculants reduced pH of packaging material environment,for example,pH finally was 7.05,lower than that of control group.Microbial inoculants accelerated transformation of ammonium nitrogen into nitrate nitrogen and reduced nitrogen loss.In addition,activities of urease and protease enhanced in packaging materials and excrements degraded rapidly.[Conclusion] The research provides technical references for strain development,selection and evaluation of fermentation bed of swine.
基金Supported by Key Science and Technology Support Program of Tianjin Municipality(13ZCZDNC00200)
文摘Beneficial microorganisms were extracted from municipal solid waste compost and treated under low temperature stress to prepare complex microbial inoc- ulants. Turfgrass was inoculated with the prepared microbial inoculants to investigate the ecological effect of ~crobial inoculants on turf storage in winter. The re- suits showed that complex low temperature tolerant microbial inoculants significandy improved plant height and aboveground biomass of turfgrass. Compared with the non-inoculated control, plant height and biomass of the first batch of inoculated turfgrass were enhanced by 6.47% and 14.32%, respectively; and they were en- hanced by 6.94% and 17.41%, respectively, for the second batch of inoculated turfgrass. Under low temperature stress in winter, the reviving rate of inoculated tarfgrass was significandy higher than that of control, which was improved by 3.34% and 43.33% for the first and second batches of inoculated turfgrass respective- ly. After low temperature storage in winter, inoculated turfgrass revived in advance; specifically, the reviving date of the second batch of inoculated turfgrass was 3 d earlier than that of control. This low temperature tolerant microbial consortium was effective for turfgrass as an ecofriendly and acceptable technology to improve plant performance and development in winter and accelerate time to market of turf.
基金Supported by Key Project of Science and Technology Support Program of Tianjin(13ZCZDNC00200)
文摘Microbial inoculants were isolated from sewage sludge and applied to turfgrass medium. Effects of microbial inoculants on seed germination and initial growth of Festuca arundinacea L. were analyzed to investigate the optimal proportion of compound microbial inoculants. Significant differences were found in various growth indices and chlorophyll content of F. arundinacea between microbial treatment and control The combination of Pseudomonas stutzeri and Trichoderma reesei exhibited the best results. Compared with the control, seed germination rate, seedling height, shoot dry weight, root weight, chlorophyll a content and chlorophyll b content of F. arundinacea treated with the combination of Pseudomonas stutzeri and Trichoderma reesei were improved by 11.60%, 43.47%, 148%, 159%, 144% and 136%, respectively. Based on comprehensive analysis of various indicators, the optimal proportion （V/V） of microbial combination was Pseudomonas stutzeri： Tfichoderma reesei = 1： 1, which was the most conducive to plant growth and development.
基金Supported by Hunan Science and Technology Innovation Program(2019NK4170)Outstanding Youth Project of Education Department of Hunan Province(18B461).
文摘Microbial inoculants have received increasing attention in strengthening plant biological barriers,antagonizing and inhibiting harmful microorganisms,and ensuring the safe production of agricultural products.This paper summarized the research status of agricultural microbial inoculants,the application of microbial inoculants in agriculture,and the trends and prospects of agricultural microbial research.
基金the National Natural Science Foundation of China(51179054)the National Science Technology Support Project(2012BAB03B03)the Ministry of Water Resources Public Welfare Projects(201301017).
文摘Wastewater treated by compound microbial inoculant(CMI)in agricultural irrigation can enrich soil fertility and decompose the possible pollutants.In this study,a greenhouse experiment using tomato as the model crop was performed to investigate the effects of treated wastewater irrigation on soil nutrients and enzymes.For this purpose,certain parameters were measured,including soil total nitrogen(N),nitrate N,total phosphorus(P),available potassium(K)and the activities of the enzymes urease,acid phosphatase and catalase in soils irrigated with fresh water,wastewater and CMI-treated wastewater under three amount of irrigation water.The results showed that irrigation with both treated and untreated wastewater significantly increased soil total N,total P,and available K,however the treated wastewater showed higher effects on soil enrichment,especially on available K.The activity of soil urease and acid phosphatase reached highest with treated wastewater irrigation,whereas wastewater irrigation increased the activity of catalase obviously.Soil enzyme and nutrient with fresh water irrigation decreased with increasing water amount;the content of soil urease,nitrate-nitrogen,total N and total P in treated wastewater and wastewater irrigation rose with increasing water amount,but the highest activity of acid phosphatase and the lowest activity of catalase were found in medium irrigation water amount.Under the condition of tomato cultivation,total N,nitrate N and total P were closely correlated with soil urease and catalase;there were significant positive correlation among soil urease,catalase,total N,nitrate N and total P;there existed significantly positive correlation between acid phosphatase and all measured soil nutrient indexes.The results suggested that irrigation with CMI-treated wastewater is a security and effective strategy to agricultural land management.
基金supported by grants from the National Key Research and Development Program of China (No.2019YFC1604701)
文摘Objective:To find a suitable ecological cultivation measure to solve the problem of root-knot nematode disease of Panax quinquefolium(Panacis Quinquefolii Radix)and the heavy metals accumulating in its roots.Methods:Three-year-old P.quinquefolium was treated with four different combinations of microbial inoculant(MI)and garbage fermentation liquid(GFL)[the joint application of‘TuXiu’MI and Fifty potassium MI(TF),the combination use of‘No.1'MI and Fifty potassium MI(NF),‘Gulefeng’poly-γ-glutamic acid MI(PGA),GFL],and the untreated control(CK).Here,high-throughput sequencing,ICP-MS and UPLC were employed to systematically characterize changes of microbial diversity and structure composition,heavy metals(As,Cd and Pb)content and ginsenoside content among different treatments.Results:The results revealed that different MIs and GFL could increase the root dry weight of P.quinquefolium,PGA enhanced it by 83.24%,followed by GFL(49.93%),meanwhile,PGA and GFL were able to lessen root-knot nematode disease incidence by 57.25%and 64.35%.The treatment of PGA and GFL can also effectively reduce heavy metals in roots.The As content in GFL and PGA was decreased by 52.17%and 43.48%respectively,while the Cd and Pb contents of GFL and PGA was decreased somewhat.Additionally,the content of total ginsenosides was increased by 42.14%and 42.07%,in response to TF and NF,respectively.Our metagenomic analysis showed that the relative abundance of particular soil microbial community members related to the biocontrol of root-knot nematode disease and plant pathogen(i.e.,Chaetomium in NF,Xylari in GFL,and Microascus in PGA),heavy metal bioremediation(Hyphomacrobium in PGA and Xylaria in GFL),and nitrogen fixation(Nordella and Nitrospira in TF)was significantly increased;notably,potential harmful microflora,such as Plectosaphaerella and Rhizobacter,were more abundant in the control group.Conclusion:MI and GFL could improve the quality of P.quinquefolium by modifying its rhizosphere microbial community structure and composition,both of them are beneficial to the development of ecological cultivation of P.quinquefolium.
基金supported by the Key Science and Technology Project of Anhui Province,China(2023n06020056)the National Natural Science Foundation of China(32071628)the Colleges and Universities Science Foundation of Anhui Province,China(2024AH020002)。
文摘Although the application of straw decomposing microorganism inoculants(SDMI)can accelerate straw decomposition,the underlying mechanisms affecting soil organic carbon(SOC)under different scenarios remain unclear.We conducted a meta-analysis using 226 observations from 86 studies on SOC changes under straw return with or without SDMI applications.Overall,our results indicated that straw with SDMI application increased the SOC stock by 1.51%at an initial carbon-to-nitrogen ratio(ICNR)>25(P<0.05),while the effect of ICNR≤25was insignificant.In particular,at ICNR>25,application of SDMI-treated straw increased SOC stocks in northern temperate continental areas(NTC)higher than in subtropical monsoon regions(STM).Furthermore,the straw with SDMI application increased higher SOC stocks in soils with pH>7.5 than those with pH≤7.5.In terms of agricultural management practices,SOC stocks were significantly higher in straw buried(SB),the experimental duration of straw return(EDSR)≥1 year,the straw return amount(SRA)>6,000 kg ha^(–1),and the SDMI application rate(SDMIR)>30 kg ha^(–1)conditions.The effect of straw with SDMI on SOC stocks under straw burying(SB)was significantly higher than that under straw mulching(SM)at ICNR≤25.At ICNR>25,EDSR,SDMIR,and the mean annual precipitation(MAP)were the main drivers of the effect of SDMI addition to straw on SOC stocks.Straw with SDMI induced SOC stock increases which increased with EDSR and decreased with increasing MAP.These findings provide a scientific basis for decision-makers and stakeholders to improve soil C management via the application of SDMI-amended straw at both regional and large scales.
文摘Agricultural areas of land are deteriorating every day owing to population increase, rapid urbanization, and industrialization. To feed today’s huge populations, increased crop production is required from smaller areas, which warrants the continuous application of high doses of inorganic fertilizers to agricultural land. These cause damage to soil health and, therefore, nutrient imbalance conditions in arable soils. Under these conditions, the benefits of microbial inoculants (such as Actinobacteria) as replacements for harmful chemicals and promoting ecofriendly sustainable farming practices have been made clear through recent technological advances. There are multifunctional traits involved in the production of different types of bioactive compounds responsible for plant growth promotion, and the biocontrol of phytopathogens has reduced the use of chemical fertilizers and pesticides. There are some well-known groups of nitrogen-fixing Actinobacteria, such as Frankia, which undergo mutualism with plants and offer enhanced symbiotic trade-offs.In addition to nitrogen fixation, increasing availability of major plant nutrients in soil due to the solubilization of immobilized forms of phosphorus and potassium compounds, production of phytohormones, such as indole-3-acetic acid, indole-3-pyruvic acid, gibberellins, and cytokinins, improving organic matter decomposition by releasing cellulases, xylanase, glucanases, lipases, and proteases, and suppression of soil-borne pathogens by the production of siderophores, ammonia, hydrogen cyanide, and chitinase are important features of Actinobacteria useful for combating biotic and abiotic stresses in plants.The positive influence of Actinobacteria on soil fertility and plant health has motivated us to compile this review of important findings associated with sustaining plant productivity in the long run.
文摘On site composting of organic household wastes is an economical and environmentally tiiendly way to manage municipal wastes. In this manuscript authors evaluated the importance of turning the wastes and of inoculating microorganisms and worms in order to unprove the composting process at domestic scale. Four treatments （Control without turning- C-, Control with Turning- CT- inoculated Mountain Microorganism with turning- MM- and Worms without turning- W-） were tested in a random experiment with tour replicates. Sixteen composting bins were fed with 300 kg of organic wastes from a local street market. The process of compostnig lasted 13 weeks after which all the composts were sifted and submitted to a range of chemical, physical and biological analysis. According to the results MM slightly increased the initial temperature and enzymatic activiry. This investigation outcome don＇t provide of sufficient grounds for a precise recommendation about worms inoculation in compost bins. A decrease between 75% and 80% in the fresh weight of the organic waste was found in the first thirteen weeks after starting the composting process in all the treatments. The quality level of the compost was acceptable, with very low heavy metal content. Turn over helps to keep the product hygienic especially after the inoculation with MM microbial starts. In conclusion and according to the results of this investigation, the use of 320 L compost bins for the organic waste management is strongly advisable.
基金National Natural Science Foundation of China(Nos.52160002,21707057,and 31860595)Natural Science Foundation of Jiangxi Province(No.20192BAB213018).
文摘Recently,returning straw to the fields has been proved as a direct and effective method to tackle soil nutrient loss and agricultural pollution.Meanwhile,the slow decomposition of straw may harm the growth of the next crop.This study aimed to determine the effects of rumen microorganisms(RMs)on straw decomposition,bacterial microbial community structure,soil properties,and soil enzyme activity.The results showed that RMs significantly enhanced the degradation rate of straw in the soil,reaching 39.52%,which was 41.37%higher than that of the control on the 30th day after straw return.After 30 d,straw degradation showed a significant slower trend in both the control and the experimental groups.According to the soil physicochemical parameters,the application of rumen fluid expedited soil matter transformation and nutrient buildup,and increased the urease,sucrase,and cellulase activity by 10%‒20%.The qualitative analysis of straw showed that the hydroxyl functional group structure of cellulose in straw was greatly damaged after the application of rumen fluid.The analysis of soil microbial community structure revealed that the addition of rumen fluid led to the proliferation of Actinobacteria with strong cellulose degradation ability,which was the main reason for the accelerated straw decomposition.Our study highlights that returning rice straw to the fields with rumen fluid inoculation can be used as an effective measure to enhance the biological value of recycled rice straw,proposing a viable solution to the problem of sluggish straw decomposition.
文摘A field experiment was carried out in Cd-contaminated rice fields in a county of Hengyang to explore the effects of different types of remediation agents on the contents of soil available Cd and rice Cd,and rice yield by using one rice variety Longliangyouhuazhan,and 4 kinds of remediation agents:DG foliar control agent,SMA microbial inoculant,XFJ organic fertilizer and LXM calcium-and silicon-based passivator as test materials.The results showed that compared with CK,after applying DG foliar control agent,soil available Cd content increased rather than decreased,whereas rice Cd content decreased,not reaching a significant level.When applied with SMA microbial inoculant,soil pH value,soil available Cd and rice Cd contents showed a downward then upward trend with the increase of its application rates.Between 3 levels of SMA treatments,the content of soil available Cd in SMA2 was the lowest,decreased by 8.59%in comparison with CK,contrarily,two other SMA treatments were increasing instead of decreasing in the content of soil available Cd.The application of XFJ organic fertilizer and LXM calcium-and silicon-based passivator increased soil pH value,and reduced soil available Cd and rice Cd contents;the contents of soil available Cd and rice Cd decreased with their application rates increasing.Compared with CK,XFJ3 reduced soil available Cd content by 9.40%,and significantly reduced rice Cd content by 57.28%.In LXM3 treatment,soil available Cd content reduced by 14.57%,rice Cd content was 71.57%lower than CK,reaching the lowest level.In general,LXM calcium-and silicon-based passivator had the best Cd reduction effect,with the optimal application amount of 6000 kg/hm^(2).
基金supported by National Research Foundation of South Africa
文摘The aim of this work was to see whether Pseudomonas putida NWU12, Pseudomonas fluorescence NWU65, Vibrio fluvialis NWU37 and Ewingella americana NWU59 are beneficial to plants and are able to promote plant growth and development when inoculated as plant growth-promoting rhizobacteria (PGPR). The four rhizobacteria were tested in vitro for PGPR activities and on spinach and pepper in pot experiments. The inoculants are all positive for ammonia (NH3 ), catalase, hydrogen cyanide (HCN), phosphate solubilization and siderophore production. Among the inoculants, E. americana NWU59 is oxidase negative. P. putida NWU12 and P. fluorescence NWU65 are producing indole-3-acetic acid (IAA). The inoculants exhibit some PGPR activities and thus tested in the screen-house. Treatments are control (water) and the four inoculants. Rhizobacterial inoculants increase spinach (17.14%-21.43%) and pepper (15.0%-37.5%) plant heights over the control. Such inoculants have the potential of improving plant yield components and may be used as biofertilizer.
文摘This work is devoted to developing of the ecologically safe technology of chickpea cultivation by using microbial biopreparations, based on the effective heterotrophic （nodulating, growth-promoting and phosphate mobilizing bacteria, and microorganisms-antagonists of the phytopathogenes） and phototrophic microorganisms （cyanobacteria）. These preparations were used for pre-sowing treatment of seeds and for treatment of the plants in the vegetation period for optimization of symbiotic nitrogen fixation and growing of ecologically safe production （without application of mineral fertilizers） in the steppe non-irrigated areas of Ukraine.
