Lepidium meyenii Walp.is a type of food used to fight fatigue,and macamides is the key anti-fatigue component in Lepidium meyenii Walp.water extract(Maca).The mechanisms of action of several foods with anti-fatigue pr...Lepidium meyenii Walp.is a type of food used to fight fatigue,and macamides is the key anti-fatigue component in Lepidium meyenii Walp.water extract(Maca).The mechanisms of action of several foods with anti-fatigue properties lie in the oxidative balance of tissues;however,little attention has been given to the microbiome.The gut microbial community is closely related to dietary choices,as well as exercise-induced skeletal muscle fatigue.As such,this study used a foodomics approach to analyze the anti-fatigue properties of maca amide,a key component in maca,on the gut microbiome.The key components in maca were identified,and after validation in a mouse model,an analysis of the gut microbiome was performed.The results of database searches and network analyses showed that the key anti-fatigue component of maca was N-benzyl-9Z,12Z-octadecadienamide(Mm).Further analyses revealed that the key microbes metabolizing Mm belonged to Alistipes,Alloprevotella,Parabacteroides,and Butyricimonas genera and the Prevotellaceae in mice.The results of microbial analysis showed that Mm could improve exercise-induced skeletal muscle fatigue in mice by modulating the L-glutamate-ornithine-proline axis through the breakdown of histidine,arginine,and proline.Therefore,from foodomics and gut microbial genomics perspectives,this study demonstrates that macamide is the key anti-fatigue component in maca,and these findings provide new insights for the development of foods that fight fatigue.展开更多
Klebsiella pneumoniae infections(KPIs),particularly carbapenem-resistant Klebsiella pneumoniae(CRKP),pose significant challenges in liver transplantation(LT)recipients,with high morbidity and mortality.Guo et al’s st...Klebsiella pneumoniae infections(KPIs),particularly carbapenem-resistant Klebsiella pneumoniae(CRKP),pose significant challenges in liver transplantation(LT)recipients,with high morbidity and mortality.Guo et al’s study highlights risk factors,such as elevated day-one alanine aminotransferase levels and prolonged catheterization,and identifies polymyxin B and ceftazidime/avibactam as effective treatments.However,limitations like the absence of pre-transplant colonization data and host-pathogen interaction insights highlight the need for enhanced strategies.Future directions should include routine CRKP colonization surveillance,immune and genomic profiling,and the development of novel therapeutics.By integrating these approaches,we can improve the prevention,diagnosis,and treatment of KPIs in LT patients.展开更多
The rapid advancement of nanopore metagenomic sequencing has revolutionized microbiome research,enabling significant breakthroughs in the study of microbial communities,ecological dynamics,and genome-level functions[1...The rapid advancement of nanopore metagenomic sequencing has revolutionized microbiome research,enabling significant breakthroughs in the study of microbial communities,ecological dynamics,and genome-level functions[1–5].This innovative sequencing technology stands out due to its ability to generate long sequencing reads,which are pivotal in resolving complex microbial genomic structures that short-read sequencing often fails to address[2,6].展开更多
This article addresses how the functionalities of the cellular machinery of a bacterium might have constrained the genomic arrangement of its genes during evolution and how we can study such problems using computation...This article addresses how the functionalities of the cellular machinery of a bacterium might have constrained the genomic arrangement of its genes during evolution and how we can study such problems using computational approaches, taking full advantage of the rapidly increasing pool of the sequenced bacterial genomes, potentially leading to a much improved understanding of why a bacterial genome is organized in the way it is. This article discusses a number of challenging computational problems in elucidating the genomic structures at multiple levels and the information that is encoded through these genomic structures, gearing towards the ultimate understanding of the governing rules of bacterial genome organization.展开更多
基金supported by the National Key Research and Development Program of China(Nos.2020YFC1606800).
文摘Lepidium meyenii Walp.is a type of food used to fight fatigue,and macamides is the key anti-fatigue component in Lepidium meyenii Walp.water extract(Maca).The mechanisms of action of several foods with anti-fatigue properties lie in the oxidative balance of tissues;however,little attention has been given to the microbiome.The gut microbial community is closely related to dietary choices,as well as exercise-induced skeletal muscle fatigue.As such,this study used a foodomics approach to analyze the anti-fatigue properties of maca amide,a key component in maca,on the gut microbiome.The key components in maca were identified,and after validation in a mouse model,an analysis of the gut microbiome was performed.The results of database searches and network analyses showed that the key anti-fatigue component of maca was N-benzyl-9Z,12Z-octadecadienamide(Mm).Further analyses revealed that the key microbes metabolizing Mm belonged to Alistipes,Alloprevotella,Parabacteroides,and Butyricimonas genera and the Prevotellaceae in mice.The results of microbial analysis showed that Mm could improve exercise-induced skeletal muscle fatigue in mice by modulating the L-glutamate-ornithine-proline axis through the breakdown of histidine,arginine,and proline.Therefore,from foodomics and gut microbial genomics perspectives,this study demonstrates that macamide is the key anti-fatigue component in maca,and these findings provide new insights for the development of foods that fight fatigue.
基金Supported by the Second Batch of Social Development Science and Technology Program Projects of Taizhou Science and Technology Bureau in 2023,No.24ywb80.
文摘Klebsiella pneumoniae infections(KPIs),particularly carbapenem-resistant Klebsiella pneumoniae(CRKP),pose significant challenges in liver transplantation(LT)recipients,with high morbidity and mortality.Guo et al’s study highlights risk factors,such as elevated day-one alanine aminotransferase levels and prolonged catheterization,and identifies polymyxin B and ceftazidime/avibactam as effective treatments.However,limitations like the absence of pre-transplant colonization data and host-pathogen interaction insights highlight the need for enhanced strategies.Future directions should include routine CRKP colonization surveillance,immune and genomic profiling,and the development of novel therapeutics.By integrating these approaches,we can improve the prevention,diagnosis,and treatment of KPIs in LT patients.
文摘The rapid advancement of nanopore metagenomic sequencing has revolutionized microbiome research,enabling significant breakthroughs in the study of microbial communities,ecological dynamics,and genome-level functions[1–5].This innovative sequencing technology stands out due to its ability to generate long sequencing reads,which are pivotal in resolving complex microbial genomic structures that short-read sequencing often fails to address[2,6].
基金supported in part by the NSF of USA (Grant Nos. DBI-0354771, ITR-IIS-0407204, DBI-0542119, CCF0621700)NIHof USA (Grant Nos. 1R01GM075331 and 1R01GM081682)the grant for the BioEnergy Science Center
文摘This article addresses how the functionalities of the cellular machinery of a bacterium might have constrained the genomic arrangement of its genes during evolution and how we can study such problems using computational approaches, taking full advantage of the rapidly increasing pool of the sequenced bacterial genomes, potentially leading to a much improved understanding of why a bacterial genome is organized in the way it is. This article discusses a number of challenging computational problems in elucidating the genomic structures at multiple levels and the information that is encoded through these genomic structures, gearing towards the ultimate understanding of the governing rules of bacterial genome organization.
