1 Introduction Genomic islands(GIs)promote co-evolution among different species,which are of significant importance for studying microbial functionality and evolution[1].To effectively identify genomic islands,numerou...1 Introduction Genomic islands(GIs)promote co-evolution among different species,which are of significant importance for studying microbial functionality and evolution[1].To effectively identify genomic islands,numerous algorithms have been developed.These methods detect candidate genomic islands either by recognizing differences in sequence composition between genomic islands and their host genomes or by analyzing multiple genomes through sequence alignment[2].However,it’s important to note that genomic islands are formed through horizontal gene transfer and typically contain functional genes,such as those related to pathogenicity,metabolic regulation,and antibiotic resistance,as well as mobile elements like insertion sequences(IS),integrative and conjugative elements(ICE),mobile genetic elements(MGE),and bacteriophages(phage).In turn,utilizing these mobile elements can aid in the identification of genomic islands.展开更多
The combination of preozonation and subsequent biological granular activated carbon （O3/BAC） filtration is well known as a promising method for the removal of many pollutants. Temperature and nutrients are the domin...The combination of preozonation and subsequent biological granular activated carbon （O3/BAC） filtration is well known as a promising method for the removal of many pollutants. Temperature and nutrients are the dominant factors in external conditions to influence the biological communities. To explore the influence of preozonation under low temperature, the factors such as dissolved oxygen （DO）, dissolved organic carbon （DOC） and NH4^＋-N were analyzed from the sampling ports every week; triphenyl tetrazolium ehloride-dehydrogenase activity （TTC-DHA） and the nitrifying activity were detected along the bed height ofbiofilter at four levels （10. 40. 70 and i 10 cm） on the 90th, 110th, and 130th day; ~nicrobial community, based on 16S rRNA gene-denaturing gradient gel electrophoresis （DGGE）, was monitored on the 130th day of the operation. The observed microbial property showed that preozonation had a positive influence on bioactivity, biomass and nitrifying activity. Community analysis showed no significant difference on the biodiversity of nitrifying bacteria between the parallel filters in the inlet end based on the method employed. This result showed that biofilters＇ performance is not correlated well with microbial biodiversity. The elevated functionality in O3/BAC filters can be a result of increased microbial activity, which was promoted by preozonation.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.62172369,62302456)the Special Support Plan for High Level Talents in Zhejiang Province(2021R52019).
文摘1 Introduction Genomic islands(GIs)promote co-evolution among different species,which are of significant importance for studying microbial functionality and evolution[1].To effectively identify genomic islands,numerous algorithms have been developed.These methods detect candidate genomic islands either by recognizing differences in sequence composition between genomic islands and their host genomes or by analyzing multiple genomes through sequence alignment[2].However,it’s important to note that genomic islands are formed through horizontal gene transfer and typically contain functional genes,such as those related to pathogenicity,metabolic regulation,and antibiotic resistance,as well as mobile elements like insertion sequences(IS),integrative and conjugative elements(ICE),mobile genetic elements(MGE),and bacteriophages(phage).In turn,utilizing these mobile elements can aid in the identification of genomic islands.
文摘The combination of preozonation and subsequent biological granular activated carbon （O3/BAC） filtration is well known as a promising method for the removal of many pollutants. Temperature and nutrients are the dominant factors in external conditions to influence the biological communities. To explore the influence of preozonation under low temperature, the factors such as dissolved oxygen （DO）, dissolved organic carbon （DOC） and NH4^＋-N were analyzed from the sampling ports every week; triphenyl tetrazolium ehloride-dehydrogenase activity （TTC-DHA） and the nitrifying activity were detected along the bed height ofbiofilter at four levels （10. 40. 70 and i 10 cm） on the 90th, 110th, and 130th day; ~nicrobial community, based on 16S rRNA gene-denaturing gradient gel electrophoresis （DGGE）, was monitored on the 130th day of the operation. The observed microbial property showed that preozonation had a positive influence on bioactivity, biomass and nitrifying activity. Community analysis showed no significant difference on the biodiversity of nitrifying bacteria between the parallel filters in the inlet end based on the method employed. This result showed that biofilters＇ performance is not correlated well with microbial biodiversity. The elevated functionality in O3/BAC filters can be a result of increased microbial activity, which was promoted by preozonation.
