The Qinghai-Tibet Plateau(QTP)distributes the largest extent of high-altitude mountain permafrost in the world(Zou et al.,2017),which has different characteristics from high-latitude permafrost(Yang et al.,2010)and st...The Qinghai-Tibet Plateau(QTP)distributes the largest extent of high-altitude mountain permafrost in the world(Zou et al.,2017),which has different characteristics from high-latitude permafrost(Yang et al.,2010)and stores massive soil carbon.展开更多
Agroecosystems in the Senegalese groundnut basin experience long periods of high temperatures and drought, which disrupt the stability of soil microbial communities. This study evaluated how that stability is affected...Agroecosystems in the Senegalese groundnut basin experience long periods of high temperatures and drought, which disrupt the stability of soil microbial communities. This study evaluated how that stability is affected by homefields and outfields’ agricultural practices and the duration of heat stress. Specifically, we collected soils from organically farmed fields that receive continual high inputs of manure (homefields), and from fields that are rarely manured (outfields). Soil samples were submitted to artificial heat stress at 60°C for 3, 14, and 28 days, followed by 28 days of recovery at 28°C. We examined the functional stability of microbial communities by quantifying C mineralization, and characterized the stability of the communities’ taxonomic compositions via high-throughput DNA sequencing. We found that the microbial communities have a low resistance to heat stress in soils from both types of fields. However, the manuring practice does affect how the functional stability of microbial communities responds to different durations of heat stress. Although functional stability was not recovered fully in either soil, microbial community resilience seemed to be greater in homefield soils. Differences in manuring practices also affected the structural taxonomic stability of microbial communities: relative abundances of Bacilli, Chloroflexia, Actinobacteria and Sordariomycetes increased in the homefield stressed-soils, but decreased significantly in outfield soils. In contrast, relative abundances of α-Proteobacteria, γ-Proteobacteria and Eurotiomycetes increased significantly in outfield stressed-soils, while decreasing significantly in the homefield soils. Relative abundances of Bacilli changed little in outfield soils, indicating that this taxon is resistant to heat stress. In summary, the microbial communities’ capacities to resist heat stress and recover from it depend upon the organic richness of the soil (i.e., manuring practice) and the adaptation of soil microbes to environmental conditions.展开更多
Reseeding the legume Caragana korshinskii,is an effective strategy to restore the vegetation in degraded desert steppe.Here,we explored the responses of soil environment and microbial communities to desert steppe degr...Reseeding the legume Caragana korshinskii,is an effective strategy to restore the vegetation in degraded desert steppe.Here,we explored the responses of soil environment and microbial communities to desert steppe degradation and C.korshinskii reseeding.A typical native-degraded-restored closed-loop system in desert steppe:the natural stage with native C.korshinskii(NC),vegetation degradation stage(bare soil)(BS),both vegetation and soil degradation stage(desertified soil)(DS)and restored stage with reseeding C.korshinskii aged eight years(RC).The results showed that,within this closed-loop system:(i)compared to BS,soil degradation(DS)resulted in a 199%increase in sand content(P<0.05),while no difference between RC and NC(P>0.05),indicating restoration of soil texture.(ii)The soil microbial structure of RC was similar to that of NC,but based on changes in abundance,it could be divided into communities that increased with the rise in soil sand content(UTC+SPP),decreased due to vegetation degradation and increased soil sand content(FOC+SEC)(P<0.05)and unchanged(UC).(iii)Microbial networks showed lower average degree and modularity after reseeding C.korshinskii compared to NC.In summary,eight years of vegetation restoration led to the recovery of soil texture and microbial community structure,but not community complexity and stability.The results emphasize the time lag for soil microbiome reconstruction after reseeding C.korshinskii,providing novel insight into plant-soil-microbial interactions during the restoration of degraded desert steppe.展开更多
基金financially supported by the National Natural Science Foundation of China(41871064)the Second Tibetan Plateau Scientific Expedition and Research Program(2019QZKK0304)。
文摘The Qinghai-Tibet Plateau(QTP)distributes the largest extent of high-altitude mountain permafrost in the world(Zou et al.,2017),which has different characteristics from high-latitude permafrost(Yang et al.,2010)and stores massive soil carbon.
文摘Agroecosystems in the Senegalese groundnut basin experience long periods of high temperatures and drought, which disrupt the stability of soil microbial communities. This study evaluated how that stability is affected by homefields and outfields’ agricultural practices and the duration of heat stress. Specifically, we collected soils from organically farmed fields that receive continual high inputs of manure (homefields), and from fields that are rarely manured (outfields). Soil samples were submitted to artificial heat stress at 60°C for 3, 14, and 28 days, followed by 28 days of recovery at 28°C. We examined the functional stability of microbial communities by quantifying C mineralization, and characterized the stability of the communities’ taxonomic compositions via high-throughput DNA sequencing. We found that the microbial communities have a low resistance to heat stress in soils from both types of fields. However, the manuring practice does affect how the functional stability of microbial communities responds to different durations of heat stress. Although functional stability was not recovered fully in either soil, microbial community resilience seemed to be greater in homefield soils. Differences in manuring practices also affected the structural taxonomic stability of microbial communities: relative abundances of Bacilli, Chloroflexia, Actinobacteria and Sordariomycetes increased in the homefield stressed-soils, but decreased significantly in outfield soils. In contrast, relative abundances of α-Proteobacteria, γ-Proteobacteria and Eurotiomycetes increased significantly in outfield stressed-soils, while decreasing significantly in the homefield soils. Relative abundances of Bacilli changed little in outfield soils, indicating that this taxon is resistant to heat stress. In summary, the microbial communities’ capacities to resist heat stress and recover from it depend upon the organic richness of the soil (i.e., manuring practice) and the adaptation of soil microbes to environmental conditions.
基金Basic Research Operating Costs of Universities Programme of China(grant no.:BR230109)the Natural Science Foundation of Inner Mongolia(grant no.:2023QN03001)the Regional Project of National Natural Science Foundation of China(grant no.:32460071,32360072).
文摘Reseeding the legume Caragana korshinskii,is an effective strategy to restore the vegetation in degraded desert steppe.Here,we explored the responses of soil environment and microbial communities to desert steppe degradation and C.korshinskii reseeding.A typical native-degraded-restored closed-loop system in desert steppe:the natural stage with native C.korshinskii(NC),vegetation degradation stage(bare soil)(BS),both vegetation and soil degradation stage(desertified soil)(DS)and restored stage with reseeding C.korshinskii aged eight years(RC).The results showed that,within this closed-loop system:(i)compared to BS,soil degradation(DS)resulted in a 199%increase in sand content(P<0.05),while no difference between RC and NC(P>0.05),indicating restoration of soil texture.(ii)The soil microbial structure of RC was similar to that of NC,but based on changes in abundance,it could be divided into communities that increased with the rise in soil sand content(UTC+SPP),decreased due to vegetation degradation and increased soil sand content(FOC+SEC)(P<0.05)and unchanged(UC).(iii)Microbial networks showed lower average degree and modularity after reseeding C.korshinskii compared to NC.In summary,eight years of vegetation restoration led to the recovery of soil texture and microbial community structure,but not community complexity and stability.The results emphasize the time lag for soil microbiome reconstruction after reseeding C.korshinskii,providing novel insight into plant-soil-microbial interactions during the restoration of degraded desert steppe.
