Microalgae-bacteria system is an emerging alternative for sustainable wastewater treatment.Exploring the structure and diversity of microbial community in microalgae-bacteria system under sulfadiazine stress can contr...Microalgae-bacteria system is an emerging alternative for sustainable wastewater treatment.Exploring the structure and diversity of microbial community in microalgae-bacteria system under sulfadiazine stress can contribute to the understanding of the sulfadiazine behavior in environments.Furthermore,as important carriers of antibiotic resistance genes(ARGs),microalgae can influence the profiles of ARGs either directly or indirectly through the secretion of metabolites.However,the effects of sulfadiazine on ARGs dissemination of microalgae-bacteria systems remain underreported.Herein,the impacts of sulfadiazine(1 mg/L)on the structural diversity and metabolic activity of microorganisms were examined in microalgae-bacteria systems.Results showed thatmicroalgae-bacteria system could remove NH_(4)^(+)-N better(about 72.3%)than activated sludge system,and hydrolysis was the first step in sulfadiazine degradation.A high level of intI1(5.7×10^(4) copies/mL)was detected in the initial media of the microalgae-bacteria system.Microalgae could hamper the rate of horizontal gene transfer activation.Compared with activated sludge system,the abundance of sul genes(sul1,sul2,sul3,and sulA)was significantly lowered after treating with microalgae-bacteria system.Additionally,the number of proteins and the sum of polysaccharides in the extracellular polymeric substances of the activated sludge system were lower than those of themicroalgae-bacteria system.Microalgae can altermicrobial communities.The genus Rozellomycota predominated all samples.Fungi with relatively high abundance increased in the microalgae-bacteria system,including Dipodascaceae,Rhodotorula,and Geotrichum.These results offer valuable insights into the application processes involving microalgae-bacteria system.展开更多
基金supported by the Key Research Program of Wuhan(No.2022022202015015)the State Key Laboratory of Urban Water Resource and Environment(Harbin Institute of Technology)(No.2022TS13)+1 种基金the key projects of National Natural Science Foundation of China(No.2019YFC0408503)Shanghai Tongji Gao Tingyao Environmental Technology Development Foundation.
文摘Microalgae-bacteria system is an emerging alternative for sustainable wastewater treatment.Exploring the structure and diversity of microbial community in microalgae-bacteria system under sulfadiazine stress can contribute to the understanding of the sulfadiazine behavior in environments.Furthermore,as important carriers of antibiotic resistance genes(ARGs),microalgae can influence the profiles of ARGs either directly or indirectly through the secretion of metabolites.However,the effects of sulfadiazine on ARGs dissemination of microalgae-bacteria systems remain underreported.Herein,the impacts of sulfadiazine(1 mg/L)on the structural diversity and metabolic activity of microorganisms were examined in microalgae-bacteria systems.Results showed thatmicroalgae-bacteria system could remove NH_(4)^(+)-N better(about 72.3%)than activated sludge system,and hydrolysis was the first step in sulfadiazine degradation.A high level of intI1(5.7×10^(4) copies/mL)was detected in the initial media of the microalgae-bacteria system.Microalgae could hamper the rate of horizontal gene transfer activation.Compared with activated sludge system,the abundance of sul genes(sul1,sul2,sul3,and sulA)was significantly lowered after treating with microalgae-bacteria system.Additionally,the number of proteins and the sum of polysaccharides in the extracellular polymeric substances of the activated sludge system were lower than those of themicroalgae-bacteria system.Microalgae can altermicrobial communities.The genus Rozellomycota predominated all samples.Fungi with relatively high abundance increased in the microalgae-bacteria system,including Dipodascaceae,Rhodotorula,and Geotrichum.These results offer valuable insights into the application processes involving microalgae-bacteria system.
