AIM:To highlight the importance of microRNA(miRNA)-21-5p in directing the phosphatase and tensin homolog(PTEN)gene to control the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR)...AIM:To highlight the importance of microRNA(miRNA)-21-5p in directing the phosphatase and tensin homolog(PTEN)gene to control the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR)pathway in retinal pigment epithelial(RPE)cells in humans subjected to photodamage.METHODS:Human adult RPE cell line-19(ARPE-19)was cultured in vitro and randomly divided into control,damage,overexpression,negative,and PI3K/Akt blocker groups to establish a photodamage model of ARPE-19 cells.The models were subjected to 24h of light exposure,after which the corresponding indices were detected.The cell counting kit-8 assay quantified cell viability,while flow cytometry determined apoptosis rates.The miRNA-21 mimics and miRNA mimic NC were transfected into ARPE-19 cells using a transient transfection technique.Quantitative reverse transcription polymerase chain reaction(SYBR Green)and Western blotting analyzed expression levels of miRNA-21-5p,PTEN,p-PI3K/PI3K,p-mTOR/mTOR,and p-Akt/Akt.Statistical analyses comprised one-way analysis of variance and the Student-Newman-Keuls test for multiple group comparisons.RESULTS:The photodamage group demonstrated reduced cell survival rates than the control group(P<0.01).The overexpression group exhibited higher cell survival rates than the injury group(P<0.01).The negative group showed no difference in viability(P>0.05).The PI3K/Akt blocker group demonstrated lower cell viability,compared with the overexpression group(P<0.01).CONCLUSION:miRNA-21-5p significantly increases ARPE-19 cell survival after photodamage and inhibits lightinduced ARPE-19 cell apoptosis,suggesting that it may play a protective role in RPE by activating the PI3K/Akt/mTOR pathway while downregulating PTEN expression.展开更多
目的探讨茵陈蒿汤对代谢相关脂肪性肝病(MAFLD)小鼠LncMEG3/miR-21-5p/PPARα介导脂肪酸β氧化的影响。方法为探讨MAFLD差异表达的miRNAs,应用PubMed、Web of Science和中国知网3个电子数据库对以中英文发表的研究进行检索,采用随机效...目的探讨茵陈蒿汤对代谢相关脂肪性肝病(MAFLD)小鼠LncMEG3/miR-21-5p/PPARα介导脂肪酸β氧化的影响。方法为探讨MAFLD差异表达的miRNAs,应用PubMed、Web of Science和中国知网3个电子数据库对以中英文发表的研究进行检索,采用随机效应模型进行Meta分析。24只C57BL/6J小鼠,SPF级,采用随机数字表法将小鼠随机分为对照组、模型组和茵陈蒿汤组,共3组,每组8只。采用喂饲高脂饲料的方法对模型组和茵陈蒿汤组小鼠建立MAFLD模型。12周后,茵陈蒿汤组给予茵陈蒿汤灌胃,每日1次,连续4周。采用HE染色法和油红O染色法观察各组小鼠肝组织病理形态表现;全自动生化分析仪检测血清甘油三酯(TG)、总胆固醇(TC)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、高密度脂蛋白胆固(HDL-C)和低密度脂蛋白胆固醇(LDL-C);试剂盒检测小鼠肝脏甘油三酯(TG)和游离脂肪酸(FFA)水平;RT-qPCR法检测小鼠LncMEG3、miR-21-5p、miR-34a-5p、miR-122-5p、miR-192-5p、过氧化物酶体增殖物激活受体α(PPARα)、肉碱棕榈酰转移酶1A(CPT1A)、肉碱棕榈酰基转移酶2(CPT2)、中链酰基辅酶A脱氢酶(MCAD)、长链脂酰辅酶A脱氢酶(LCAD)、酰基辅酶A氧化酶1(ACOX1)和三羟酰辅酶A脱氢酶(EHHADH)mRNA水平蛋白表达水平。结果Meta分析结果显示,与健康对照组相比,MAFLD组miR-34a-5p、miR-122-5p、miR-192-5p、miR-21-5p、miR-451-5p水平显著升高。肝脏HE染色结果发现,模型组小鼠出现肝细胞肿胀,体积增大,有明显脂肪变性;与模型组相比,茵陈蒿汤组小鼠肝细胞体积肿胀明显降低,脂肪变性显著减少。肝脏油红O染色结果发现,与对照组相比,模型组肝脏可见大量橘红色脂滴,脂质沉积显著升高;茵陈蒿汤组可见少量脂肪变性,橘红色脂滴分布减少,肝脏脂质沉积情况明显改善。相较于对照组,模型组小鼠血清HDL-C、LDL-C、TG、TC、AST、ALT表达显著升高(P<0.05),肝脏TG和FFA水平均显著升高(P<0.05);相较于模型组,茵陈蒿汤组小鼠肝脏FFA和TG水平显著降低(P<0.05),血清AST、LDL-C、TG、ALT水平显著降低(P<0.05)。与对照组相比,模型组miR-21-5p、miR-34a-5p、miR-122-5p、miR-192-5p表达水平增高(P<0.05);与模型组相比,茵陈蒿汤组miR-21-5p、miR-34a-5p、miR-122-5p、miR-192-5p表达水平降低(P<0.05),miR-21-5p变化倍数(模型组/茵陈蒿汤组)最大。与对照组相比,模型组Lnc MEG3表达水平降低(P<0.05);与模型组相比,茵陈蒿汤组Lnc MEG3表达水平升高(P<0.05)。与对照组相比,模型组PPARα、CPT1A、CPT2、MCAD、LCAD、ACOX1、EHHADH mRNA和蛋白表达水平显著降低(P<0.05);与模型组相比,茵陈蒿汤组PPARα、CPT1A、CPT2、MCAD、LCAD、ACOX1、EHHADH mRNA和蛋白表达水平显著升高(P<0.05)。结论茵陈蒿汤可能通过调控Lnc MEG3/miR-21-5p/PPARα信号通路促进肝脏脂肪酸β氧化,从而达到防治MAFLD的作用。展开更多
目的:探讨免疫球蛋白A肾病(IgAN)患者血浆中微小核糖核酸-21-5p(miR-21-5p)表达与肾功能及肾间质纤维化(RIF)的关系。方法:选取2021年1月—2023年1月我院收治IgAN患者100例为IgAN组,根据慢性肾脏病分期分为1期组22例、2期组30例、3期组2...目的:探讨免疫球蛋白A肾病(IgAN)患者血浆中微小核糖核酸-21-5p(miR-21-5p)表达与肾功能及肾间质纤维化(RIF)的关系。方法:选取2021年1月—2023年1月我院收治IgAN患者100例为IgAN组,根据慢性肾脏病分期分为1期组22例、2期组30例、3期组26例、4期组14例、5期组8例,根据牛津分型分为T0组32例、T1组42例、T2组26例,另选取同期50名体检健康志愿者为对照组。检测血浆miR-21-5p、肾功能指标、RIF指标。分析IgAN患者血浆miR-21-5p表达与肾功能及RIF指标的相关性。结果:与对照组比较,IgAN组血浆miR-21-5p、24 h尿蛋白定量(24 h UPQ)、单核细胞趋化蛋白-1(MCP-1)、转化生长因子-β_(1)(TGF-β_(1))水平升高,估计肾小球滤过率(eGFR)降低(P<0.05)。随着慢性肾脏病分期及牛津分型的增加,血浆miR-21-5p、24 h UPQ、MCP-1、TGF-β_(1)水平依次升高,eGFR依次降低(P<0.05)。IgAN患者血浆miR-21-5p表达与eGFR呈负相关,与24 h UPQ、MCP-1、TGF-β_(1)水平呈正相关(P<0.05)。结论:IgAN患者血浆miR-21-5p高表达,与肾功能降低和RIF密切相关。展开更多
文摘AIM:To highlight the importance of microRNA(miRNA)-21-5p in directing the phosphatase and tensin homolog(PTEN)gene to control the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR)pathway in retinal pigment epithelial(RPE)cells in humans subjected to photodamage.METHODS:Human adult RPE cell line-19(ARPE-19)was cultured in vitro and randomly divided into control,damage,overexpression,negative,and PI3K/Akt blocker groups to establish a photodamage model of ARPE-19 cells.The models were subjected to 24h of light exposure,after which the corresponding indices were detected.The cell counting kit-8 assay quantified cell viability,while flow cytometry determined apoptosis rates.The miRNA-21 mimics and miRNA mimic NC were transfected into ARPE-19 cells using a transient transfection technique.Quantitative reverse transcription polymerase chain reaction(SYBR Green)and Western blotting analyzed expression levels of miRNA-21-5p,PTEN,p-PI3K/PI3K,p-mTOR/mTOR,and p-Akt/Akt.Statistical analyses comprised one-way analysis of variance and the Student-Newman-Keuls test for multiple group comparisons.RESULTS:The photodamage group demonstrated reduced cell survival rates than the control group(P<0.01).The overexpression group exhibited higher cell survival rates than the injury group(P<0.01).The negative group showed no difference in viability(P>0.05).The PI3K/Akt blocker group demonstrated lower cell viability,compared with the overexpression group(P<0.01).CONCLUSION:miRNA-21-5p significantly increases ARPE-19 cell survival after photodamage and inhibits lightinduced ARPE-19 cell apoptosis,suggesting that it may play a protective role in RPE by activating the PI3K/Akt/mTOR pathway while downregulating PTEN expression.
文摘目的:探讨免疫球蛋白A肾病(IgAN)患者血浆中微小核糖核酸-21-5p(miR-21-5p)表达与肾功能及肾间质纤维化(RIF)的关系。方法:选取2021年1月—2023年1月我院收治IgAN患者100例为IgAN组,根据慢性肾脏病分期分为1期组22例、2期组30例、3期组26例、4期组14例、5期组8例,根据牛津分型分为T0组32例、T1组42例、T2组26例,另选取同期50名体检健康志愿者为对照组。检测血浆miR-21-5p、肾功能指标、RIF指标。分析IgAN患者血浆miR-21-5p表达与肾功能及RIF指标的相关性。结果:与对照组比较,IgAN组血浆miR-21-5p、24 h尿蛋白定量(24 h UPQ)、单核细胞趋化蛋白-1(MCP-1)、转化生长因子-β_(1)(TGF-β_(1))水平升高,估计肾小球滤过率(eGFR)降低(P<0.05)。随着慢性肾脏病分期及牛津分型的增加,血浆miR-21-5p、24 h UPQ、MCP-1、TGF-β_(1)水平依次升高,eGFR依次降低(P<0.05)。IgAN患者血浆miR-21-5p表达与eGFR呈负相关,与24 h UPQ、MCP-1、TGF-β_(1)水平呈正相关(P<0.05)。结论:IgAN患者血浆miR-21-5p高表达,与肾功能降低和RIF密切相关。
