目的:探讨结肠癌患者组织中microRNA-99a表达水平以及对结肠癌细胞增殖和迁移的影响。方法:取南京医科大学附属常州二院胃肠病中心49例结肠癌患者肿瘤组织、癌旁组织(癌旁5cm)标本以及结肠癌细胞HCT-116、HT-29、SW-480、Caco-2和正常...目的:探讨结肠癌患者组织中microRNA-99a表达水平以及对结肠癌细胞增殖和迁移的影响。方法:取南京医科大学附属常州二院胃肠病中心49例结肠癌患者肿瘤组织、癌旁组织(癌旁5cm)标本以及结肠癌细胞HCT-116、HT-29、SW-480、Caco-2和正常结肠上皮细胞HCoe Pic,采用实时荧光定量PCR检测结肠癌患者肿瘤组织和结肠癌细胞中microRNA-99a表达水平;结肠癌细胞株HT-29转染microRNA-99a抑制剂后,CCK-8法检测microRNA-99a对结肠癌细胞增殖的变化;transwell法观察microRNA-99a对结肠癌细胞迁移的影响;Western blotting检测了HT-29中FGFR-3的表达水平。结果:microRNA-99a表达在结肠癌组织中明显高于癌旁组织(6.27±0.48 vs 1.34±0.54,P<0.05)、在肿瘤细胞中明显高于正常结肠上皮细胞(5.48±0.34,7.67±0.24,5.78±0.22,6.28±0.44 vs 1.45±0.37,P<0.05)。转染microRNA-99a抑制剂后,HT-29细胞的增殖和迁移能力均明显下降(P<0.05);同时,HT-29中FGFR-3显著降低(P<0.05)。结论:microRNA-99a在结肠癌组织中高表达,低表达microRNA-99a可减弱结肠癌细胞的增殖和迁移能力,且可能通过FGFR-3信号通路发挥作用。展开更多
This study proposes a method for^(99)Mo production via electron accelerator irradiation of a natural-uranium-bearing liquid molten salt target,with advantages including low nuclear proliferation risk,online extraction...This study proposes a method for^(99)Mo production via electron accelerator irradiation of a natural-uranium-bearing liquid molten salt target,with advantages including low nuclear proliferation risk,online extraction capability,and low construction costs.The approach primarily produces^(99)Mo through photofission of uranium(~95%),specifically^(238)U(γ,f).Secondary neutrons,originating from photonuclear interactions or fission processes,contribute minimally(~5%)to^(99)Mo production owing to their high energies and low fission cross sections.Key parameter analyses revealed that fluoride salt systems exhibit higher^(99)Mo yield.Their performance stems from high bremsstrahlung energy loss rate and superior photon yield,making them optimal molten salt target materials.To maximize photofission and photoneutron cross sections while minimizing highenergy gamma ray shielding requirements,an electron beam energy range of 40-80 MeV is recommended.To suppress local hot spots and prevent molten salt boiling,flow conditions were introduced to enhance convective heat transfer,effectively reducing the peak temperature.At a flow velocity of 0.5 m/s and under 80 MeV energy conditions,the maximum system temperature is only 808.9 K,which is significantly lower than the boiling point of 1773 K.Under optimized parameters,the maximum annual production capacity of~(99)Mo reaches 4486.49 Ci,sufficient for millions of diagnostic procedures and equivalent to 16.37% of China's projected demand for 2030.This method provides a viable pathway for stable,large-scale^(99)Mo production.展开更多
文摘目的:探讨结肠癌患者组织中microRNA-99a表达水平以及对结肠癌细胞增殖和迁移的影响。方法:取南京医科大学附属常州二院胃肠病中心49例结肠癌患者肿瘤组织、癌旁组织(癌旁5cm)标本以及结肠癌细胞HCT-116、HT-29、SW-480、Caco-2和正常结肠上皮细胞HCoe Pic,采用实时荧光定量PCR检测结肠癌患者肿瘤组织和结肠癌细胞中microRNA-99a表达水平;结肠癌细胞株HT-29转染microRNA-99a抑制剂后,CCK-8法检测microRNA-99a对结肠癌细胞增殖的变化;transwell法观察microRNA-99a对结肠癌细胞迁移的影响;Western blotting检测了HT-29中FGFR-3的表达水平。结果:microRNA-99a表达在结肠癌组织中明显高于癌旁组织(6.27±0.48 vs 1.34±0.54,P<0.05)、在肿瘤细胞中明显高于正常结肠上皮细胞(5.48±0.34,7.67±0.24,5.78±0.22,6.28±0.44 vs 1.45±0.37,P<0.05)。转染microRNA-99a抑制剂后,HT-29细胞的增殖和迁移能力均明显下降(P<0.05);同时,HT-29中FGFR-3显著降低(P<0.05)。结论:microRNA-99a在结肠癌组织中高表达,低表达microRNA-99a可减弱结肠癌细胞的增殖和迁移能力,且可能通过FGFR-3信号通路发挥作用。
基金supported by the National Natural Science Foundation of China(Nos.12435012,12175300,and 12475185)Shanghai Natural Science Foundation(No.24ZR1478500)Nuclear energy development project(HNKF202210(24))。
文摘This study proposes a method for^(99)Mo production via electron accelerator irradiation of a natural-uranium-bearing liquid molten salt target,with advantages including low nuclear proliferation risk,online extraction capability,and low construction costs.The approach primarily produces^(99)Mo through photofission of uranium(~95%),specifically^(238)U(γ,f).Secondary neutrons,originating from photonuclear interactions or fission processes,contribute minimally(~5%)to^(99)Mo production owing to their high energies and low fission cross sections.Key parameter analyses revealed that fluoride salt systems exhibit higher^(99)Mo yield.Their performance stems from high bremsstrahlung energy loss rate and superior photon yield,making them optimal molten salt target materials.To maximize photofission and photoneutron cross sections while minimizing highenergy gamma ray shielding requirements,an electron beam energy range of 40-80 MeV is recommended.To suppress local hot spots and prevent molten salt boiling,flow conditions were introduced to enhance convective heat transfer,effectively reducing the peak temperature.At a flow velocity of 0.5 m/s and under 80 MeV energy conditions,the maximum system temperature is only 808.9 K,which is significantly lower than the boiling point of 1773 K.Under optimized parameters,the maximum annual production capacity of~(99)Mo reaches 4486.49 Ci,sufficient for millions of diagnostic procedures and equivalent to 16.37% of China's projected demand for 2030.This method provides a viable pathway for stable,large-scale^(99)Mo production.
