目的探讨microRNA-31对脊髓损伤后胃肠动力因素的作用。方法将36只microRNA-31转基因小鼠和36只FVB小鼠分别设为对照组和模型组,用Impactor M-Ⅲ脊髓撞击器建立小鼠脊髓损伤模型。用BBB运动功能评分评估小鼠下肢的恢复情况。HE染色观察...目的探讨microRNA-31对脊髓损伤后胃肠动力因素的作用。方法将36只microRNA-31转基因小鼠和36只FVB小鼠分别设为对照组和模型组,用Impactor M-Ⅲ脊髓撞击器建立小鼠脊髓损伤模型。用BBB运动功能评分评估小鼠下肢的恢复情况。HE染色观察脊髓组织的变化。用Real-time PCR法检测生长抑素受体(SSTR2)mRNA的表达,用免疫荧光法检测一氧化氮合酶(iNOS)蛋白和SSTR2蛋白的表达。结果造模后第3天、第7天和第14天,脊髓组织破坏、坏死、空泡形成,胶原纤维明显增加。造模后第14天,FVB模型组与对照组比较,SSTR2 m RNA的表达明显升高(P<0.05),iNOS蛋白和SSTR2蛋白的表达量明显升高;microRNA-31转基因小鼠模型组与对照组比较,SSTR2 mRNA的表达明显升高(P<0.05),iNOS蛋白和SSTR2蛋白的表达量明显升高;microRNA-31转基因小鼠模型组与FVB模型组比较,SSTR2 m RNA的表达明显降低(P<0.05),iNOS蛋白和SSTR2蛋白的表达量明显降低。结论脊髓损伤后胃肠动力障碍的发生可能与SSTR2 m RNA的表达上调、SSTR2蛋白和i NOS蛋白的表达增高有关。高表达的microRNA-31可能与脊髓损伤后胃肠动力障碍的恢复有关。展开更多
MicroRNAs have been increasingly recognized as useful biomarkers for colorectal cancers(CRC).We have recently observed that microRNA-31(miR-31)expression is associated with BRAF mutation and prognosis in CRC.Moreover,...MicroRNAs have been increasingly recognized as useful biomarkers for colorectal cancers(CRC).We have recently observed that microRNA-31(miR-31)expression is associated with BRAF mutation and prognosis in CRC.Moreover,high miR-31 expression is frequently detected in sessile serrated adenomas compared with hyperplastic polyps(HPs).These results suggest that miR-31 may contribute to the progression of serrated lesions.At a follow-up colonoscopy,we observed the case of a 75-year-old man with a 7-mm flat-elevated lesion in the cecum and diagnosed the lesion as an early invasive carcinoma with serrated features.Tissue specimens were obtained from the representative areas to compare the molecular alterations in the carcinoma component with those in the HP component.Higher miR-31 expression was observed in the carcinoma component(57-fold increase)and the HP component(8-fold increase)compared with the paired normal mucosa,suggesting that miR-31 may be one of the key molecules in serrated pathway progression.展开更多
文摘目的探讨microRNA-31对脊髓损伤后胃肠动力因素的作用。方法将36只microRNA-31转基因小鼠和36只FVB小鼠分别设为对照组和模型组,用Impactor M-Ⅲ脊髓撞击器建立小鼠脊髓损伤模型。用BBB运动功能评分评估小鼠下肢的恢复情况。HE染色观察脊髓组织的变化。用Real-time PCR法检测生长抑素受体(SSTR2)mRNA的表达,用免疫荧光法检测一氧化氮合酶(iNOS)蛋白和SSTR2蛋白的表达。结果造模后第3天、第7天和第14天,脊髓组织破坏、坏死、空泡形成,胶原纤维明显增加。造模后第14天,FVB模型组与对照组比较,SSTR2 m RNA的表达明显升高(P<0.05),iNOS蛋白和SSTR2蛋白的表达量明显升高;microRNA-31转基因小鼠模型组与对照组比较,SSTR2 mRNA的表达明显升高(P<0.05),iNOS蛋白和SSTR2蛋白的表达量明显升高;microRNA-31转基因小鼠模型组与FVB模型组比较,SSTR2 m RNA的表达明显降低(P<0.05),iNOS蛋白和SSTR2蛋白的表达量明显降低。结论脊髓损伤后胃肠动力障碍的发生可能与SSTR2 m RNA的表达上调、SSTR2蛋白和i NOS蛋白的表达增高有关。高表达的microRNA-31可能与脊髓损伤后胃肠动力障碍的恢复有关。
基金Supported by Japan Society for the Promotion of Science(JSPS)Grant-in-Aid for Scientific Research,grant No.23790800(to Nosho K)A-STEP(Adaptable and Seamless Technology Transfer Program through Target-driven R and D)(to Nosho K)
文摘MicroRNAs have been increasingly recognized as useful biomarkers for colorectal cancers(CRC).We have recently observed that microRNA-31(miR-31)expression is associated with BRAF mutation and prognosis in CRC.Moreover,high miR-31 expression is frequently detected in sessile serrated adenomas compared with hyperplastic polyps(HPs).These results suggest that miR-31 may contribute to the progression of serrated lesions.At a follow-up colonoscopy,we observed the case of a 75-year-old man with a 7-mm flat-elevated lesion in the cecum and diagnosed the lesion as an early invasive carcinoma with serrated features.Tissue specimens were obtained from the representative areas to compare the molecular alterations in the carcinoma component with those in the HP component.Higher miR-31 expression was observed in the carcinoma component(57-fold increase)and the HP component(8-fold increase)compared with the paired normal mucosa,suggesting that miR-31 may be one of the key molecules in serrated pathway progression.
