BACKGROUND Studies have reported that microRNA-30c(miR-30c)has vital functions in the development and progression of multiple cancers.AIM To investigate the clinical significance and role of miR-30c in pancreatic canc...BACKGROUND Studies have reported that microRNA-30c(miR-30c)has vital functions in the development and progression of multiple cancers.AIM To investigate the clinical significance and role of miR-30c in pancreatic cancer.METHODS MiR-30c and twinfilin 1(TWF1)expression levels were analyzed in Gene Expression Omnibus datasets and validated in human pancreatic cancer by quantitative real-time polymerase chain reaction(RT-qPCR).The effects of miR-30c on pancreatic cancer cell growth,apoptosis,and cell cycle were evaluated by CCK-8 and flow cytometry assays.Furthermore,the in vivo effects were investigated using a subcutaneous xenograft experiment.Target gene prediction software and luciferase reporter assays were used to identify TWF1 as a direct target of miR-30c.RESULTS The expression of miR-30c was significantly decreased in pancreatic cancer tissues and associated with survival.Gain-and loss-of-function assays showed that miR-30c suppressed pancreatic cancer cell proliferation in vitro and in vivo.RT-qPCR,Western blot,and luciferase reporter assays showed that miR-30c directly targeted TWF1.The expression level of miR-30c was negatively correlated with TWF1 expression in pancreatic cancer tissues.Furthermore,the effects of ectopic miR-30c were rescued by TWF1 overexpression.CONCLUSION Our results identified the role of the miR-30c/TWF1 axis in pancreatic cancer progression and demonstrated that miR-30c might serve as a prognostic biomarker and therapeutic target for pancreatic cancer.展开更多
介绍开展数智融合技术支持下的初中英语分级阅读教学研究的背景、现状及意义。分析C30教育云平台的功能特点。提出数智融合技术支持下的初中英语分级阅读教学“6S策略”。以《HI CLASSICS品读文学经典》中的故事类绘本A Little Princes...介绍开展数智融合技术支持下的初中英语分级阅读教学研究的背景、现状及意义。分析C30教育云平台的功能特点。提出数智融合技术支持下的初中英语分级阅读教学“6S策略”。以《HI CLASSICS品读文学经典》中的故事类绘本A Little Princess为例,借助C30教育云平台,探索以“6S策略”为指引、以“D+7R”模式为导向的初中英语分级阅读教学新模式,通过课前预学、课上共学、课后延学三大板块、三管齐下,创设更高效的人工智能数字化课堂,为师生提供更优质的教学体验。展开更多
基金Supported by the National Nature Science Foundation of China,No.61802350
文摘BACKGROUND Studies have reported that microRNA-30c(miR-30c)has vital functions in the development and progression of multiple cancers.AIM To investigate the clinical significance and role of miR-30c in pancreatic cancer.METHODS MiR-30c and twinfilin 1(TWF1)expression levels were analyzed in Gene Expression Omnibus datasets and validated in human pancreatic cancer by quantitative real-time polymerase chain reaction(RT-qPCR).The effects of miR-30c on pancreatic cancer cell growth,apoptosis,and cell cycle were evaluated by CCK-8 and flow cytometry assays.Furthermore,the in vivo effects were investigated using a subcutaneous xenograft experiment.Target gene prediction software and luciferase reporter assays were used to identify TWF1 as a direct target of miR-30c.RESULTS The expression of miR-30c was significantly decreased in pancreatic cancer tissues and associated with survival.Gain-and loss-of-function assays showed that miR-30c suppressed pancreatic cancer cell proliferation in vitro and in vivo.RT-qPCR,Western blot,and luciferase reporter assays showed that miR-30c directly targeted TWF1.The expression level of miR-30c was negatively correlated with TWF1 expression in pancreatic cancer tissues.Furthermore,the effects of ectopic miR-30c were rescued by TWF1 overexpression.CONCLUSION Our results identified the role of the miR-30c/TWF1 axis in pancreatic cancer progression and demonstrated that miR-30c might serve as a prognostic biomarker and therapeutic target for pancreatic cancer.
文摘介绍开展数智融合技术支持下的初中英语分级阅读教学研究的背景、现状及意义。分析C30教育云平台的功能特点。提出数智融合技术支持下的初中英语分级阅读教学“6S策略”。以《HI CLASSICS品读文学经典》中的故事类绘本A Little Princess为例,借助C30教育云平台,探索以“6S策略”为指引、以“D+7R”模式为导向的初中英语分级阅读教学新模式,通过课前预学、课上共学、课后延学三大板块、三管齐下,创设更高效的人工智能数字化课堂,为师生提供更优质的教学体验。
