Objective: To investigate clinical signii cance of microRNA-130b(mi R-130b) in osteosarcoma and its role in cell growth and invasion. Methods: miR-130b expression was detected in 68 samples of surgically resected oste...Objective: To investigate clinical signii cance of microRNA-130b(mi R-130b) in osteosarcoma and its role in cell growth and invasion. Methods: miR-130b expression was detected in 68 samples of surgically resected osteosarcoma and matched normal tumor-adjacent tissues by q RT-PCR. The expression of miR-130b was altered by corresponding vectors in osteosarcoma cells, and then Western blot was used to detect the expression of PPAR毭. Brd U cell proliferation and Transwell assays were performed to determine cell proliferation and invasion. Results: The expression of miR-130b in osteosarcoma tissues was signii cantly higher than that in normal tumor-adjacent tissues. Its expression in patients with metastasis was signii cantly higher than that in those without metastases. miR-130b expression in tumor tissues was signii cantly associated with tumor size, clinical stage and distant metastasis. And its expression was signii cantly correlated with overall survival and disease free survival. miR-130b overexpression obviously repressed the expression of PPAR毭, and resulted in signii cant increase of Saos-2 cell proliferation and invasion. On the contrast, repressing miR-130b expression with its inhibitor signii cantly increased PPAR毭 expression, and inhibited MG-63 cell proliferation and invasion. Conclusions: The high-expression of miR-130b is correlated with the adverse clinicopathological features and poor prognosis in osteosarcoma. miR-130b may regulate proliferation and invasion of osteosarcoma cells by targeting PPAR毭, suggesting miR-130b may play a key role in the progression of osteosarcoma.展开更多
BACKGROUND Studies have reported that microRNA-30c(miR-30c)has vital functions in the development and progression of multiple cancers.AIM To investigate the clinical significance and role of miR-30c in pancreatic canc...BACKGROUND Studies have reported that microRNA-30c(miR-30c)has vital functions in the development and progression of multiple cancers.AIM To investigate the clinical significance and role of miR-30c in pancreatic cancer.METHODS MiR-30c and twinfilin 1(TWF1)expression levels were analyzed in Gene Expression Omnibus datasets and validated in human pancreatic cancer by quantitative real-time polymerase chain reaction(RT-qPCR).The effects of miR-30c on pancreatic cancer cell growth,apoptosis,and cell cycle were evaluated by CCK-8 and flow cytometry assays.Furthermore,the in vivo effects were investigated using a subcutaneous xenograft experiment.Target gene prediction software and luciferase reporter assays were used to identify TWF1 as a direct target of miR-30c.RESULTS The expression of miR-30c was significantly decreased in pancreatic cancer tissues and associated with survival.Gain-and loss-of-function assays showed that miR-30c suppressed pancreatic cancer cell proliferation in vitro and in vivo.RT-qPCR,Western blot,and luciferase reporter assays showed that miR-30c directly targeted TWF1.The expression level of miR-30c was negatively correlated with TWF1 expression in pancreatic cancer tissues.Furthermore,the effects of ectopic miR-30c were rescued by TWF1 overexpression.CONCLUSION Our results identified the role of the miR-30c/TWF1 axis in pancreatic cancer progression and demonstrated that miR-30c might serve as a prognostic biomarker and therapeutic target for pancreatic cancer.展开更多
文摘Objective: To investigate clinical signii cance of microRNA-130b(mi R-130b) in osteosarcoma and its role in cell growth and invasion. Methods: miR-130b expression was detected in 68 samples of surgically resected osteosarcoma and matched normal tumor-adjacent tissues by q RT-PCR. The expression of miR-130b was altered by corresponding vectors in osteosarcoma cells, and then Western blot was used to detect the expression of PPAR毭. Brd U cell proliferation and Transwell assays were performed to determine cell proliferation and invasion. Results: The expression of miR-130b in osteosarcoma tissues was signii cantly higher than that in normal tumor-adjacent tissues. Its expression in patients with metastasis was signii cantly higher than that in those without metastases. miR-130b expression in tumor tissues was signii cantly associated with tumor size, clinical stage and distant metastasis. And its expression was signii cantly correlated with overall survival and disease free survival. miR-130b overexpression obviously repressed the expression of PPAR毭, and resulted in signii cant increase of Saos-2 cell proliferation and invasion. On the contrast, repressing miR-130b expression with its inhibitor signii cantly increased PPAR毭 expression, and inhibited MG-63 cell proliferation and invasion. Conclusions: The high-expression of miR-130b is correlated with the adverse clinicopathological features and poor prognosis in osteosarcoma. miR-130b may regulate proliferation and invasion of osteosarcoma cells by targeting PPAR毭, suggesting miR-130b may play a key role in the progression of osteosarcoma.
基金Supported by the National Nature Science Foundation of China,No.61802350
文摘BACKGROUND Studies have reported that microRNA-30c(miR-30c)has vital functions in the development and progression of multiple cancers.AIM To investigate the clinical significance and role of miR-30c in pancreatic cancer.METHODS MiR-30c and twinfilin 1(TWF1)expression levels were analyzed in Gene Expression Omnibus datasets and validated in human pancreatic cancer by quantitative real-time polymerase chain reaction(RT-qPCR).The effects of miR-30c on pancreatic cancer cell growth,apoptosis,and cell cycle were evaluated by CCK-8 and flow cytometry assays.Furthermore,the in vivo effects were investigated using a subcutaneous xenograft experiment.Target gene prediction software and luciferase reporter assays were used to identify TWF1 as a direct target of miR-30c.RESULTS The expression of miR-30c was significantly decreased in pancreatic cancer tissues and associated with survival.Gain-and loss-of-function assays showed that miR-30c suppressed pancreatic cancer cell proliferation in vitro and in vivo.RT-qPCR,Western blot,and luciferase reporter assays showed that miR-30c directly targeted TWF1.The expression level of miR-30c was negatively correlated with TWF1 expression in pancreatic cancer tissues.Furthermore,the effects of ectopic miR-30c were rescued by TWF1 overexpression.CONCLUSION Our results identified the role of the miR-30c/TWF1 axis in pancreatic cancer progression and demonstrated that miR-30c might serve as a prognostic biomarker and therapeutic target for pancreatic cancer.
