MicroRNAs(miRNAs), small non-coding RNAs, are involved in many aspects of biological processes. Previous studies have indicated that mi RNAs are important for hair follicle development and growth. In our study, we fou...MicroRNAs(miRNAs), small non-coding RNAs, are involved in many aspects of biological processes. Previous studies have indicated that mi RNAs are important for hair follicle development and growth. In our study, we found by q RT-PCR that miR-148 b was signi ficantly upregulated in sheep wool follicle bulbs in anagen phase compared with the telogen phase of the hair follicle cycle. Overexpression of miR-148 b promoted proliferation of both HHDPC and HHGMC. By using the TOPFlash system we demonstrated that miR-148 b could activate Wnt/β-catenin pathway and b-catenin, cyc D, c-jun and PPARD were consistently upregulated accordingly.Furthermore, transcript factor nuclear factor of activated T cells type 5(NFAT5) and Wnt10 b were predicted to be the target of mi R-148 b and this was substantiated using a Dual-Luciferase reporter system. Subsequently NFAT5 was further identi fied as the target of mi R-148 b using western blotting. These results were considered to indicate that mi R-148 b could activate the Wnt/β-catenin signal pathway by targeting NFAT5 to promote the proliferation of human hair follicle cells.展开更多
目的研究miR-21在弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达,探讨miR-21表达与DL-BCL临床病理特征的关系及其在DLBCL发生发展中的意义。方法采用Real-time RT-PCR方法检测36例DLBCL和10例正常淋巴结中miR-21的表...目的研究miR-21在弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达,探讨miR-21表达与DL-BCL临床病理特征的关系及其在DLBCL发生发展中的意义。方法采用Real-time RT-PCR方法检测36例DLBCL和10例正常淋巴结中miR-21的表达,并采用免疫组织化学SP法检测Ki-67、PTEN在DLBCL中的表达。结果miR-21在DLBCL中高表达,36例DLBCL中有14例表达PTEN(38.9%),21例Ki-67≥50%(58.3%)。DLBCL中miR-21表达水平与PTEN蛋白表达呈负相关,其高表达与DLBCL高Ann Arbor分期、高增殖指数(Ki-67≥50%)、国际预后指数(IPI)呈正相关。结论miR-21过表达可能是DLBCL恶性度高的标志,是促进DLBCL肿瘤细胞增殖的重要因素。PTEN可能是miR-21在DLBCL发挥作用的靶标。展开更多
基金supported by grants from the National High Technology Research and Development Program of China(2013AA102506)Regional Project of National Natural Science Foundation of China (31360534)
文摘MicroRNAs(miRNAs), small non-coding RNAs, are involved in many aspects of biological processes. Previous studies have indicated that mi RNAs are important for hair follicle development and growth. In our study, we found by q RT-PCR that miR-148 b was signi ficantly upregulated in sheep wool follicle bulbs in anagen phase compared with the telogen phase of the hair follicle cycle. Overexpression of miR-148 b promoted proliferation of both HHDPC and HHGMC. By using the TOPFlash system we demonstrated that miR-148 b could activate Wnt/β-catenin pathway and b-catenin, cyc D, c-jun and PPARD were consistently upregulated accordingly.Furthermore, transcript factor nuclear factor of activated T cells type 5(NFAT5) and Wnt10 b were predicted to be the target of mi R-148 b and this was substantiated using a Dual-Luciferase reporter system. Subsequently NFAT5 was further identi fied as the target of mi R-148 b using western blotting. These results were considered to indicate that mi R-148 b could activate the Wnt/β-catenin signal pathway by targeting NFAT5 to promote the proliferation of human hair follicle cells.
