目的分析冠状动脉病变患者血清微小RNA(miR)-128-3p,miRNA-126表达与Gensini积分的相关性。方法选取哈励逊国际和平医院心内科2020年6月~2022年接诊的60例冠状动脉病变患者展开回顾性研究,将其设为观察组;另选取该院同期接诊的60例非冠...目的分析冠状动脉病变患者血清微小RNA(miR)-128-3p,miRNA-126表达与Gensini积分的相关性。方法选取哈励逊国际和平医院心内科2020年6月~2022年接诊的60例冠状动脉病变患者展开回顾性研究,将其设为观察组;另选取该院同期接诊的60例非冠状动脉病变患者,将其设为对照组,检测、比较两组血清miR-128-3p,miR-126和Gensini积分;比较观察组中单支、双支、多支病变患者血清miR-128-3p,miR-126和Gensini积分;根据冠状动脉狭窄率分为Ⅰ度、Ⅱ度、Ⅲ度和Ⅳ度组,比较不同狭窄分级组血清miR-128-3p,miR-126和Gensini积分;根据Gensini积分分为轻度病变组和重度病变组,比较轻度病变组、重度病变组血清miR-128-3p,miR-126;Pearson分析血清miR-128-3p,miR-126与Gensini积分的相关性;绘制受试者工作(ROC)曲线,计算曲线下面积(AUC),分析血清miR-128-3p,miR-126联合检测对冠状动脉病变的预测效能。Logistic回归分析冠状动脉病变的影响因素。结果与对照组比较,观察组血清miR-128-3p(4.28±0.52 vs 2.61±0.36),Gensini积分(31.29±5.62分vs 6.16±1.04分)升高(t=21.678,34.058),血清miR-126(11.05±3.16 vs 19.62±5.14)降低(t=11.002),差异具有统计学意义(均P<0.05)。与双支病变、单支病变组比较,多支病变组血清miR-128-3p,Gensini积分升高(t=4.945,7.171;6.795,11.686),血清miR-126降低(t=3.104,5.033),差异具有统计学意义(均P<0.05)。与Ⅰ~Ⅱ级组比较,Ⅲ~Ⅳ级组血清miR-128-3p,Gensini积分升高(t=5.590,12.961),血清miR-126降低(t=6.021),差异具有统计学意义(均P<0.05)。与轻度病变组比较,重度病变组血清miR-128-3p升高(t=4.056),血清miR-126降低(t=4.806),差异具有统计学意义(均P<0.05)。血清miR-128-3p与Gensini积分呈正相关性(r=0.404,P<0.05),血清miR-126与Gensini积分呈负相关性(r=-0.393,P<0.05)。血清miR-128-3p,miR-126联合检测的AUC高于单一检测,差异具有统计学意义(Z=2.768,2.152,均P<0.05)。观察组吸烟、三酰甘油(TG)、血尿酸(UA)、纤维蛋白原(FIB)与对照组比较,差异具有统计学意义(t/χ^(2)=4.231~28.732,均P<0.05)。结论冠状动脉病变患者血清miR-128-3p呈高表达,血清miR-126呈低表达,与Gensini积分存在一定的相关性,血清miR-128-3p和miR-126联合检测可提高对冠状动脉病变的预测效能,且冠状动脉病变的发生与多种因素有关,应当引起临床重视。展开更多
目的:探讨微小RNA-128-3p及微小RNA-381-3p对于下肢动脉硬化闭塞症患者的预测价值及相关因素分析。方法:选取2024年2月至6月青岛市市立医院本部院区血管外科共72例下肢动脉硬化闭塞症患者纳入实验组;同时随机选取健康志愿者共36例纳入...目的:探讨微小RNA-128-3p及微小RNA-381-3p对于下肢动脉硬化闭塞症患者的预测价值及相关因素分析。方法:选取2024年2月至6月青岛市市立医院本部院区血管外科共72例下肢动脉硬化闭塞症患者纳入实验组;同时随机选取健康志愿者共36例纳入对照组。采用实时荧光定量聚合酶链式反应(qRT-PCR)法检测血清miRNA-128-3p及miRNA-381-3p水平,采用酶联免疫吸附测定(ELISA)法检测血清白细胞介素(IL)-6、IL-8、金属蛋白酶组织抑制因子-1 (TIMP-1)水平。结果:下肢动脉硬化患者血清miRNA-128-3p及miRNA-381-3p的表达水平相较于健康人群来说明显上升,差异有统计学意义(p 0.05)。miRNA-128-3p、miRNA-381-3p、白介素-6、白介素-8、TIMP-1均为下肢动脉硬化的危险因素(p Objective: To explore the predictive value of microRNA-128-3p and microRNA-381-3p for patients with lower extremity arteriosclerosis obliterans and analyze the related factors. Methods: A total of 72 patients with lower extremity arteriosclerosis obliterans from the Department of Vascular Surgery of the Main Campus of Qingdao Municipal Hospital from February to June 2024 were included in the experimental group;simultaneously, 36 healthy volunteers were randomly selected and included in the control group. The levels of serum miRNA-128-3p and miRNA-381-3p were detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), and the levels of serum interleukin (IL)-6, IL-8, and tissue inhibitor of metalloproteinase-1 (TIMP-1) were detected by enzyme-linked immunosorbent assay (ELISA). Results: The expression levels of miRNA-128-3p and miRNA-381-3p in the serum of patients with lower extremity arteriosclerosis were significantly higher than those in healthy individuals, with statistically significant differences (p 0.05). miRNA-128-3p, miRNA-381-3p, IL-6, IL-8, and TIMP-1 were all risk factors for lower extremity arteriosclerosis (p < 0.05), but they were not supported as predictive factors for disease progression. Conclusion: miRNA-128-3p and miRNA-381-3p can be used as specific biomarkers for predicting lower extremity arteriosclerosis.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated mortality.Efficient therapeutic targets are beneficial to treat and predict the disease.Currently,the exact mo...BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated mortality.Efficient therapeutic targets are beneficial to treat and predict the disease.Currently,the exact molecular mechanisms leading to the progression of HCC are still unclear.Research has shown that the microRNA-142-3p level decreases in HCC,whereas bioinformatics analysis of the cancer genome atlas database shows the ASH1L expression increased among liver tumor tissues.In this paper,we will explore the effects and mechanisms of microRNA-142-3p and ASH1L affect the prognosis of HCC patients and HCC cell bioactivity,and the association between them.AIM To investigate the effects and mechanisms of microRNA-142-3p and ASH1L on the HCC cell bioactivity and prognosis of HCC patients.METHODS In this study,we grouped HCC patients according to their immunohistochemistry results of ASH1L with pathological tissues,and retrospectively analyzed the prognosis of HCC patients.Furthermore,explored the roles and mechanisms of microRNA-142-3p and ASH1L by cellular and animal experiments,which involved the following experimental methods:Immunohistochemical staining,western blot,quantitative real-time-polymerase chain reaction,flow cytometric analysis,tumor xenografts in nude mice,etc.The statistical methods involved in this study contained t-test,one-way analysis of variance,theχ^(2)test,the Kaplan-Meier approach and the log-rank test.RESULTS In this study,we found that HCC patients with high expression of ASH1L possess a more recurrence rate as well as a decreased overall survival rate.ASH1L promotes the tumorigenicity of HCC and microRNA-142-3p exhibits reduced expression in HCC tissues and interacts with ASH1L through targeting the ASH1L 3′untranslated region.Furthermore,microRNA-142-3p promotes apoptosis and inhibits proliferation,invasion,and migration of HCC cell lines in vitro via ASH1L.For the exploration mechanism,we found ASH1L may promote an immunosuppressive microenvironment in HCC and ASH1L affects the expression of the cell junction protein zonula occludens-1,which is potentially relevant to the immune system.CONCLUSION Loss function of microRNA-142-3p induces cancer progression and immune evasion through upregulation of ASH1L in HCC.Both microRNA-142-3p and ASH1L can feature as new biomarker for HCC in the future.展开更多
Objective To investigate the differential expression of microRNA-144-3p in endometrial cells exposed to copper ions in vitro.The specific mechanism by which microRNA-144-3p is involved in Cu^(2+)-induced damage to the...Objective To investigate the differential expression of microRNA-144-3p in endometrial cells exposed to copper ions in vitro.The specific mechanism by which microRNA-144-3p is involved in Cu^(2+)-induced damage to the human endometrial epithelial cells(HEECs)was explored.Methods HEECs were cultured in copper-containing culture medium to simulate changes in the endometrium after copper intrauterine device(Cu-IUD)implantation.Reverse transcription quantitative PCR(RT-qPCR)was used to detect the differential expression of miR-144-3p in HEECs after Cu^(2+)treatment.MiRNAs,siRNAs and related inhibitors were used to treat HEECs.The expression levels of related downstream genes were then analyzed by RT-qPCR,Western blotting and immunofluorescence to explore the specific mechanism involved.Results MiR-144-3p was significantly upregulated in the Cu^(2+)-treated HEECs.The expression of P-NF-κB,MMP9,TGF-β3 and P-SMAD3 was significantly decreased in HEECs treated with 10μg/mL Cu^(2+).MiR-144-3p regulated the expression of metallothionein 1A(MT1A)and thrombospondin-1(THBS-1)in Cu^(2+)-treated HEECs.The expression of P-NF-κB can be regulated by MT1A,and an inhibitor of P-NF-κB can significantly reduce the expression of MMP9 in Cu^(2+)-treated HEECs.The expression of TGF-β3 can be regulated by THBS-1,and a TGF-β3 inhibitor can significantly reduce the expression of SMAD3 in Cu^(2+)-treated HEECs.The proliferative capacity of HEECs treated with MMP9 or SMAD3 inhibitors was significantly reduced.Conclusions The increased Cu^(2+)concentration led to the upregulation of miR-144-3p,further reducing the expression levels of its target genes(MT1A and THBS-1),which in turn downregulated the expression of NF-κB,MMP9,TGF-β3 and SMAD3,ultimately leading to increased endometrial cell damage and decreased cell proliferation.展开更多
文摘目的分析冠状动脉病变患者血清微小RNA(miR)-128-3p,miRNA-126表达与Gensini积分的相关性。方法选取哈励逊国际和平医院心内科2020年6月~2022年接诊的60例冠状动脉病变患者展开回顾性研究,将其设为观察组;另选取该院同期接诊的60例非冠状动脉病变患者,将其设为对照组,检测、比较两组血清miR-128-3p,miR-126和Gensini积分;比较观察组中单支、双支、多支病变患者血清miR-128-3p,miR-126和Gensini积分;根据冠状动脉狭窄率分为Ⅰ度、Ⅱ度、Ⅲ度和Ⅳ度组,比较不同狭窄分级组血清miR-128-3p,miR-126和Gensini积分;根据Gensini积分分为轻度病变组和重度病变组,比较轻度病变组、重度病变组血清miR-128-3p,miR-126;Pearson分析血清miR-128-3p,miR-126与Gensini积分的相关性;绘制受试者工作(ROC)曲线,计算曲线下面积(AUC),分析血清miR-128-3p,miR-126联合检测对冠状动脉病变的预测效能。Logistic回归分析冠状动脉病变的影响因素。结果与对照组比较,观察组血清miR-128-3p(4.28±0.52 vs 2.61±0.36),Gensini积分(31.29±5.62分vs 6.16±1.04分)升高(t=21.678,34.058),血清miR-126(11.05±3.16 vs 19.62±5.14)降低(t=11.002),差异具有统计学意义(均P<0.05)。与双支病变、单支病变组比较,多支病变组血清miR-128-3p,Gensini积分升高(t=4.945,7.171;6.795,11.686),血清miR-126降低(t=3.104,5.033),差异具有统计学意义(均P<0.05)。与Ⅰ~Ⅱ级组比较,Ⅲ~Ⅳ级组血清miR-128-3p,Gensini积分升高(t=5.590,12.961),血清miR-126降低(t=6.021),差异具有统计学意义(均P<0.05)。与轻度病变组比较,重度病变组血清miR-128-3p升高(t=4.056),血清miR-126降低(t=4.806),差异具有统计学意义(均P<0.05)。血清miR-128-3p与Gensini积分呈正相关性(r=0.404,P<0.05),血清miR-126与Gensini积分呈负相关性(r=-0.393,P<0.05)。血清miR-128-3p,miR-126联合检测的AUC高于单一检测,差异具有统计学意义(Z=2.768,2.152,均P<0.05)。观察组吸烟、三酰甘油(TG)、血尿酸(UA)、纤维蛋白原(FIB)与对照组比较,差异具有统计学意义(t/χ^(2)=4.231~28.732,均P<0.05)。结论冠状动脉病变患者血清miR-128-3p呈高表达,血清miR-126呈低表达,与Gensini积分存在一定的相关性,血清miR-128-3p和miR-126联合检测可提高对冠状动脉病变的预测效能,且冠状动脉病变的发生与多种因素有关,应当引起临床重视。
文摘目的:探讨微小RNA-128-3p及微小RNA-381-3p对于下肢动脉硬化闭塞症患者的预测价值及相关因素分析。方法:选取2024年2月至6月青岛市市立医院本部院区血管外科共72例下肢动脉硬化闭塞症患者纳入实验组;同时随机选取健康志愿者共36例纳入对照组。采用实时荧光定量聚合酶链式反应(qRT-PCR)法检测血清miRNA-128-3p及miRNA-381-3p水平,采用酶联免疫吸附测定(ELISA)法检测血清白细胞介素(IL)-6、IL-8、金属蛋白酶组织抑制因子-1 (TIMP-1)水平。结果:下肢动脉硬化患者血清miRNA-128-3p及miRNA-381-3p的表达水平相较于健康人群来说明显上升,差异有统计学意义(p 0.05)。miRNA-128-3p、miRNA-381-3p、白介素-6、白介素-8、TIMP-1均为下肢动脉硬化的危险因素(p Objective: To explore the predictive value of microRNA-128-3p and microRNA-381-3p for patients with lower extremity arteriosclerosis obliterans and analyze the related factors. Methods: A total of 72 patients with lower extremity arteriosclerosis obliterans from the Department of Vascular Surgery of the Main Campus of Qingdao Municipal Hospital from February to June 2024 were included in the experimental group;simultaneously, 36 healthy volunteers were randomly selected and included in the control group. The levels of serum miRNA-128-3p and miRNA-381-3p were detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), and the levels of serum interleukin (IL)-6, IL-8, and tissue inhibitor of metalloproteinase-1 (TIMP-1) were detected by enzyme-linked immunosorbent assay (ELISA). Results: The expression levels of miRNA-128-3p and miRNA-381-3p in the serum of patients with lower extremity arteriosclerosis were significantly higher than those in healthy individuals, with statistically significant differences (p 0.05). miRNA-128-3p, miRNA-381-3p, IL-6, IL-8, and TIMP-1 were all risk factors for lower extremity arteriosclerosis (p < 0.05), but they were not supported as predictive factors for disease progression. Conclusion: miRNA-128-3p and miRNA-381-3p can be used as specific biomarkers for predicting lower extremity arteriosclerosis.
基金Supported by the Haihe Laboratory of Cell Ecosystem Innovation Fund,No.22HHXBJC00001the Key Discipline Special Project of Tianjin Municipal Health Commission,No.TJWJ2022XK016.
文摘BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated mortality.Efficient therapeutic targets are beneficial to treat and predict the disease.Currently,the exact molecular mechanisms leading to the progression of HCC are still unclear.Research has shown that the microRNA-142-3p level decreases in HCC,whereas bioinformatics analysis of the cancer genome atlas database shows the ASH1L expression increased among liver tumor tissues.In this paper,we will explore the effects and mechanisms of microRNA-142-3p and ASH1L affect the prognosis of HCC patients and HCC cell bioactivity,and the association between them.AIM To investigate the effects and mechanisms of microRNA-142-3p and ASH1L on the HCC cell bioactivity and prognosis of HCC patients.METHODS In this study,we grouped HCC patients according to their immunohistochemistry results of ASH1L with pathological tissues,and retrospectively analyzed the prognosis of HCC patients.Furthermore,explored the roles and mechanisms of microRNA-142-3p and ASH1L by cellular and animal experiments,which involved the following experimental methods:Immunohistochemical staining,western blot,quantitative real-time-polymerase chain reaction,flow cytometric analysis,tumor xenografts in nude mice,etc.The statistical methods involved in this study contained t-test,one-way analysis of variance,theχ^(2)test,the Kaplan-Meier approach and the log-rank test.RESULTS In this study,we found that HCC patients with high expression of ASH1L possess a more recurrence rate as well as a decreased overall survival rate.ASH1L promotes the tumorigenicity of HCC and microRNA-142-3p exhibits reduced expression in HCC tissues and interacts with ASH1L through targeting the ASH1L 3′untranslated region.Furthermore,microRNA-142-3p promotes apoptosis and inhibits proliferation,invasion,and migration of HCC cell lines in vitro via ASH1L.For the exploration mechanism,we found ASH1L may promote an immunosuppressive microenvironment in HCC and ASH1L affects the expression of the cell junction protein zonula occludens-1,which is potentially relevant to the immune system.CONCLUSION Loss function of microRNA-142-3p induces cancer progression and immune evasion through upregulation of ASH1L in HCC.Both microRNA-142-3p and ASH1L can feature as new biomarker for HCC in the future.
文摘Objective To investigate the differential expression of microRNA-144-3p in endometrial cells exposed to copper ions in vitro.The specific mechanism by which microRNA-144-3p is involved in Cu^(2+)-induced damage to the human endometrial epithelial cells(HEECs)was explored.Methods HEECs were cultured in copper-containing culture medium to simulate changes in the endometrium after copper intrauterine device(Cu-IUD)implantation.Reverse transcription quantitative PCR(RT-qPCR)was used to detect the differential expression of miR-144-3p in HEECs after Cu^(2+)treatment.MiRNAs,siRNAs and related inhibitors were used to treat HEECs.The expression levels of related downstream genes were then analyzed by RT-qPCR,Western blotting and immunofluorescence to explore the specific mechanism involved.Results MiR-144-3p was significantly upregulated in the Cu^(2+)-treated HEECs.The expression of P-NF-κB,MMP9,TGF-β3 and P-SMAD3 was significantly decreased in HEECs treated with 10μg/mL Cu^(2+).MiR-144-3p regulated the expression of metallothionein 1A(MT1A)and thrombospondin-1(THBS-1)in Cu^(2+)-treated HEECs.The expression of P-NF-κB can be regulated by MT1A,and an inhibitor of P-NF-κB can significantly reduce the expression of MMP9 in Cu^(2+)-treated HEECs.The expression of TGF-β3 can be regulated by THBS-1,and a TGF-β3 inhibitor can significantly reduce the expression of SMAD3 in Cu^(2+)-treated HEECs.The proliferative capacity of HEECs treated with MMP9 or SMAD3 inhibitors was significantly reduced.Conclusions The increased Cu^(2+)concentration led to the upregulation of miR-144-3p,further reducing the expression levels of its target genes(MT1A and THBS-1),which in turn downregulated the expression of NF-κB,MMP9,TGF-β3 and SMAD3,ultimately leading to increased endometrial cell damage and decreased cell proliferation.
