Virus spread is closely related to pathogenesis.Traditional research methods of virus spread do not distinguish cell-to-cell spread from cell-free spread.The uncertainty of virus induced plaque and virus spread direct...Virus spread is closely related to pathogenesis.Traditional research methods of virus spread do not distinguish cell-to-cell spread from cell-free spread.The uncertainty of virus induced plaque and virus spread direction makes it difficult to track the spread of virus in situ.Herein,the cellular network was fabricated on the basis of PEG modification and soft lithography.Therein,cell numbers and spatial distributions we re p recisely controlled,e.g.,cellular netwo rks like "WHU" can be achieved.Furthermore,a micro-injector was combined with the cellular network for virus spread on the fixed point,in which virus spread direction was limited in one dimension.The results suggested that controllable and flexible cellular network can be constructed on the PEG pattern.The synergia of micro-injector and cellular network provides an advanced tool to investigate virus cell-to-cell spread at the initial infection stage.展开更多
基金the National Science and Technology Major Project of China(No. 2018ZX10301405)the National Natural Science Foundation of China(Nos. 21775111, 21475099)
文摘Virus spread is closely related to pathogenesis.Traditional research methods of virus spread do not distinguish cell-to-cell spread from cell-free spread.The uncertainty of virus induced plaque and virus spread direction makes it difficult to track the spread of virus in situ.Herein,the cellular network was fabricated on the basis of PEG modification and soft lithography.Therein,cell numbers and spatial distributions we re p recisely controlled,e.g.,cellular netwo rks like "WHU" can be achieved.Furthermore,a micro-injector was combined with the cellular network for virus spread on the fixed point,in which virus spread direction was limited in one dimension.The results suggested that controllable and flexible cellular network can be constructed on the PEG pattern.The synergia of micro-injector and cellular network provides an advanced tool to investigate virus cell-to-cell spread at the initial infection stage.
