Micro-eukaryotic communities are highly sensitive to environmental changes, and their structure offers crucial insights into the impact of human activities on aquatic habitats. Despite their significance, the assembly...Micro-eukaryotic communities are highly sensitive to environmental changes, and their structure offers crucial insights into the impact of human activities on aquatic habitats. Despite their significance, the assembly mechanisms of micro-eukaryotic communities in lake ecosystems heavily influenced by human activities are not well understood. In this study, we utilized 18S rDNA amplicon sequencing to examine the biodiversity of micro-eukaryotes over a one-year period in Lake Weishan, one of the largest freshwater lakes in northern China. We analyzed the ecological processes governing community assembly among key ecological groups, specifically habitat generalists and specialists. Our findings revealed substantial temporal variation in microbial diversity and community composition, likely driven by water temperature, dissolved oxygen, and the concentrations of several ions (e.g., Ca^(2+) and F^(−)). Notably, community assembly in this canal-disturbed lake was predominantly influenced by stochastic processes (58.18%) rather than deterministic processes (20.46%). Habitat specialists (425 operational taxonomic units, OTUs) exhibited significantly greater diversity compared to habitat generalists (189 OTUs) and were predominantly shaped by stochastic processes (52.47%). In contrast, habitat generalists were less influenced by these processes (17.69%). Altogether, these results suggest that the ecological processes governing micro-eukaryotic community assembly differ markedly between habitat generalists and specialists, primarily due to their varied adaptability to environmental changes.展开更多
Freshwater ecosystems harbor a vast diversity of micro-eukaryotes(rotifers,crustaceans and protists),and such diverse taxonomic groups play important roles in ecosystem functioning and services.Unfortunately,freshwate...Freshwater ecosystems harbor a vast diversity of micro-eukaryotes(rotifers,crustaceans and protists),and such diverse taxonomic groups play important roles in ecosystem functioning and services.Unfortunately,freshwater ecosystems and biodiversity therein are threatened by many environmental stressors,particularly those derived from intensive human activities such as chemical pollution.In the past several decades,significant efforts have been devoted to halting biodiversity loss to recover services and functioning of freshwater ecosystems.Biodiversity monitoring is the first and a crucial step towards diagnosing pollution impacts on ecosystems and making conservation plans.Yet,bio-monitoring of ubiquitous micro-eukaryotes is extremely challenging,owing to many technical issues associated with micro-zooplankton such as microscopic size,fuzzy morphological features,and extremely high biodiversity.Here,we review current methods used for monitoring zooplankton biodiversity to advance management of impaired freshwater ecosystems.We discuss the development of traditional morphologybased identification methods such as scanning electron microscope(SEM)and ZOOSCAN and FlowCAM automatic systems,and DNA-based strategies such as metabarcoding and real-time quantitative PCR.In addition,we summarize advantages and disadvantages of these methods when applied for monitoring impacted ecosystems,and we propose practical DNA-based monitoring workflows for studying biological consequences of environmental pollution in freshwater ecosystems.Finally,we propose possible solutions for existing technical issues to improve accuracy and efficiency of DNA-based biodiversity monitoring.展开更多
基金supported by the National Natural Science Foundation of China(No.32030015,32470545)Science&Technology Fundamental Resources Investigation Program(Grant No.2022FY100400)+1 种基金the Key Projects of Science and Technology Research from the Education Department of Hubei Province(D20221002)the Project(No:RSP2025R7)of the King Saud University,Saudi Arabia.
文摘Micro-eukaryotic communities are highly sensitive to environmental changes, and their structure offers crucial insights into the impact of human activities on aquatic habitats. Despite their significance, the assembly mechanisms of micro-eukaryotic communities in lake ecosystems heavily influenced by human activities are not well understood. In this study, we utilized 18S rDNA amplicon sequencing to examine the biodiversity of micro-eukaryotes over a one-year period in Lake Weishan, one of the largest freshwater lakes in northern China. We analyzed the ecological processes governing community assembly among key ecological groups, specifically habitat generalists and specialists. Our findings revealed substantial temporal variation in microbial diversity and community composition, likely driven by water temperature, dissolved oxygen, and the concentrations of several ions (e.g., Ca^(2+) and F^(−)). Notably, community assembly in this canal-disturbed lake was predominantly influenced by stochastic processes (58.18%) rather than deterministic processes (20.46%). Habitat specialists (425 operational taxonomic units, OTUs) exhibited significantly greater diversity compared to habitat generalists (189 OTUs) and were predominantly shaped by stochastic processes (52.47%). In contrast, habitat generalists were less influenced by these processes (17.69%). Altogether, these results suggest that the ecological processes governing micro-eukaryotic community assembly differ markedly between habitat generalists and specialists, primarily due to their varied adaptability to environmental changes.
基金supported by the National Natural Science Foundation of China[grant numbers 31800307,31572228]National Key R&D Program of China[grant number 2016YFC0500406]Chinese Academy of Science[grant number ZDRW-ZS-2016-5-6].
文摘Freshwater ecosystems harbor a vast diversity of micro-eukaryotes(rotifers,crustaceans and protists),and such diverse taxonomic groups play important roles in ecosystem functioning and services.Unfortunately,freshwater ecosystems and biodiversity therein are threatened by many environmental stressors,particularly those derived from intensive human activities such as chemical pollution.In the past several decades,significant efforts have been devoted to halting biodiversity loss to recover services and functioning of freshwater ecosystems.Biodiversity monitoring is the first and a crucial step towards diagnosing pollution impacts on ecosystems and making conservation plans.Yet,bio-monitoring of ubiquitous micro-eukaryotes is extremely challenging,owing to many technical issues associated with micro-zooplankton such as microscopic size,fuzzy morphological features,and extremely high biodiversity.Here,we review current methods used for monitoring zooplankton biodiversity to advance management of impaired freshwater ecosystems.We discuss the development of traditional morphologybased identification methods such as scanning electron microscope(SEM)and ZOOSCAN and FlowCAM automatic systems,and DNA-based strategies such as metabarcoding and real-time quantitative PCR.In addition,we summarize advantages and disadvantages of these methods when applied for monitoring impacted ecosystems,and we propose practical DNA-based monitoring workflows for studying biological consequences of environmental pollution in freshwater ecosystems.Finally,we propose possible solutions for existing technical issues to improve accuracy and efficiency of DNA-based biodiversity monitoring.
