BACKGROUND The molecular mechanisms involved in micro RNAs(mi RNAs)have been extensively investigated in gastric cancer(GC).However,how mi R-331 regulates GC pathogenesis remains unknown.AIM To illuminate the effect o...BACKGROUND The molecular mechanisms involved in micro RNAs(mi RNAs)have been extensively investigated in gastric cancer(GC).However,how mi R-331 regulates GC pathogenesis remains unknown.AIM To illuminate the effect of mi R-331 on cell metastasis and tumor growth in GC.METHODS The q RT-PCR,CCK8,Transwell,cell adhesion,Western blot,luciferase reporter and xenograft tumor formation assays were applied to explore the regulatory mechanism of mi R-331 in GC.RESULTS Downregulation of mi R-331 associated with poor prognosis was detected in GC.Functionally,mi R-331 suppressed cell proliferation,metastasis and tumor growth in GC.Further,mi R-331 was verified to directly target musashi1(MSI1).In addition,mi R-331 inversely regulated MSI1 expression in GC tissues.Furthermore,upregulation of MSI1 weakened the inhibitory effect of mi R-331 in GC.CONCLUSION mi R-331 inhibited development of GC through targeting MSI1,which may be used as an indicator for the prediction and prognosis of GC.展开更多
目的分析血清micro RNA-23a(mi R-23a)与阻塞性睡眠呼吸暂停低通气综合征(OSAHS)儿童发生脑损伤的关系。方法收集2018年1月—2020年6月本院接受治疗的69例发生脑损伤的OSAHS患儿资料(发生组),并收集同期于本院接受治疗的69例未发生脑损...目的分析血清micro RNA-23a(mi R-23a)与阻塞性睡眠呼吸暂停低通气综合征(OSAHS)儿童发生脑损伤的关系。方法收集2018年1月—2020年6月本院接受治疗的69例发生脑损伤的OSAHS患儿资料(发生组),并收集同期于本院接受治疗的69例未发生脑损伤的OSAHS患儿(未发生组)临床资料进行回顾性对比分析。全部患儿均在治疗前接受全面的实验室指标检查,重点分析治疗前血清mi R-23a m RNA相对表达量与患儿发生脑损伤的关系及各指标对发生脑损伤的预测价值。结果发生组mi R-23a m RNA相对表达量高于未发生组(P<0.05);回归分析结果显示,治疗前血清mi R-23a m RNA相对表达量异常与OSAHS患儿发生脑损伤有关,治疗前血清mi R-23a m RNA相对表达量高表达可能是OSAHS患儿发生脑损伤风险因子(OR>1,P<0.05);绘制ROC曲线结果显示,治疗前血清mi R-23a m RNA相对表达量预测OSAHS患儿发生脑损伤风险的AUC>0.80,预测价值较理想。结论OSAHS患儿接受治疗前血清mi R-23a m RNA相对表达量与脑损伤发生有关,可能是OSAHS患儿发生脑损伤的风险因子,对患儿脑损伤有一定预测价值。展开更多
文摘BACKGROUND The molecular mechanisms involved in micro RNAs(mi RNAs)have been extensively investigated in gastric cancer(GC).However,how mi R-331 regulates GC pathogenesis remains unknown.AIM To illuminate the effect of mi R-331 on cell metastasis and tumor growth in GC.METHODS The q RT-PCR,CCK8,Transwell,cell adhesion,Western blot,luciferase reporter and xenograft tumor formation assays were applied to explore the regulatory mechanism of mi R-331 in GC.RESULTS Downregulation of mi R-331 associated with poor prognosis was detected in GC.Functionally,mi R-331 suppressed cell proliferation,metastasis and tumor growth in GC.Further,mi R-331 was verified to directly target musashi1(MSI1).In addition,mi R-331 inversely regulated MSI1 expression in GC tissues.Furthermore,upregulation of MSI1 weakened the inhibitory effect of mi R-331 in GC.CONCLUSION mi R-331 inhibited development of GC through targeting MSI1,which may be used as an indicator for the prediction and prognosis of GC.
文摘目的分析血清micro RNA-23a(mi R-23a)与阻塞性睡眠呼吸暂停低通气综合征(OSAHS)儿童发生脑损伤的关系。方法收集2018年1月—2020年6月本院接受治疗的69例发生脑损伤的OSAHS患儿资料(发生组),并收集同期于本院接受治疗的69例未发生脑损伤的OSAHS患儿(未发生组)临床资料进行回顾性对比分析。全部患儿均在治疗前接受全面的实验室指标检查,重点分析治疗前血清mi R-23a m RNA相对表达量与患儿发生脑损伤的关系及各指标对发生脑损伤的预测价值。结果发生组mi R-23a m RNA相对表达量高于未发生组(P<0.05);回归分析结果显示,治疗前血清mi R-23a m RNA相对表达量异常与OSAHS患儿发生脑损伤有关,治疗前血清mi R-23a m RNA相对表达量高表达可能是OSAHS患儿发生脑损伤风险因子(OR>1,P<0.05);绘制ROC曲线结果显示,治疗前血清mi R-23a m RNA相对表达量预测OSAHS患儿发生脑损伤风险的AUC>0.80,预测价值较理想。结论OSAHS患儿接受治疗前血清mi R-23a m RNA相对表达量与脑损伤发生有关,可能是OSAHS患儿发生脑损伤的风险因子,对患儿脑损伤有一定预测价值。
