目的探讨缺氧缺血性脑病新生儿血清miR-27a、miR-17-5p、miR-181a表达水平及其临床意义。方法选取2018年3月至2024年3月石家庄市妇幼保健院收治的184例缺氧缺血性脑病新生儿为研究组,随访6个月后,根据随访结果将其分为预后良好组和预后...目的探讨缺氧缺血性脑病新生儿血清miR-27a、miR-17-5p、miR-181a表达水平及其临床意义。方法选取2018年3月至2024年3月石家庄市妇幼保健院收治的184例缺氧缺血性脑病新生儿为研究组,随访6个月后,根据随访结果将其分为预后良好组和预后不良组;另选取同期在石家庄市妇幼保健院分娩的205例健康新生儿作为对照组。采用实时荧光定量PCR(qRT-PCR)测定各组血清miR-27a、miR-17-5p、miR-181a的相对表达水平。绘制受试者工作特征(ROC)曲线分析血清miR-27a、miR-17-5p、miR-181a单独及3项联合对缺氧缺血性脑病新生儿预后不良的预测价值。采用多因素Logistic回归分析缺氧缺血性脑病新生儿预后不良的影响因素。结果与对照组相比,研究组血清miR-27a、miR-17-5p表达水平降低,miR-181a表达水平升高,差异均有统计学意义(P<0.05)。6个月随访结果显示,预后良好组有128例,预后不良组有56例;与预后良好组相比,预后不良组血清miR-27a、miR-17-5p表达水平降低,miR-181a表达水平、宫内窘迫占比、1 min Apgar评分<4分占比升高,差异均有统计学意义(P<0.05)。ROC曲线分析结果显示,血清miR-27a、miR-17-5p、miR-181a联合预测缺氧缺血性脑病新生儿预后不良的曲线下面积(AUC)大于miR-27a、miR-17-5p、miR-181a单独预测的AUC(Z=3.041、3.587、2.907,均P<0.05)。多因素Logistic回归分析结果显示,宫内窘迫、1 min Apgar评分及miR-181a、miR-27a、miR-17-5p表达水平均为缺氧缺血性脑病新生儿预后不良的影响因素(P<0.05)。结论缺氧缺血性脑病新生儿血清miR-27a、miR-17-5p呈低表达,miR-181a呈高表达,且均为预后不良的影响因素,三者联合检测能提高缺氧缺血新生儿预后不良的预测效能。展开更多
BACKGROUND Gastric cancer(GC)is among the most common malignant tumors and remains a leading cause of cancer-related mortality worldwide.Furthermore,exosomal miRNAs are regarded as promising noninvasive biomarkers for...BACKGROUND Gastric cancer(GC)is among the most common malignant tumors and remains a leading cause of cancer-related mortality worldwide.Furthermore,exosomal miRNAs are regarded as promising noninvasive biomarkers for diagnosing malignant tumors.AIM To investigate the expression of exosomal miR-17-92 clusters and develop a potential biomarker for GC diagnosis METHODS Exosomes were isolated from serum samples obtained from 72 GC patients and 20 healthy controls.The isolated exosomes were validated using transmission electron microscopy,nanoparticle tracking analysis,and western blotting.Exosomal RNA was then extracted,and the expression profile of the miR-17-92 cluster was analyzed using qRT-PCR.Statistical methods were employed to evaluate the relationship between the serum exosomal miR-17-92 cluster expre-ssion and the clinicopathological parameters of GC patients as well as to assess the diagnostic utility of these miRNAs.RESULTS The expression of four members of the exosomal miR-17-92 cluster-miR-17,miR-18,miR-19a,and miR-92-was significantly upregulated in the serum samples of patients with GC compared with those of healthy controls.The miR-17-92 cluster panel demonstrated substantially higher clinical diagnostic value for GC than any individual component or pair.Additionally,the expression of traditional tumor biomarkers-carcinoembryonic antigen and carbohydrate antigen 19-9-was significantly elevated in the serum of patients with GC compared with that of healthy controls.Each biomarker,whether alone or in combination,effectively differentiated the patients from healthy controls.Furthermore,a combined panel of the two traditional tumor biomarkers and the four miR-17-92 cluster members exhibited the highest diagnostic accuracy for GC.Elevated miR-17-92 expression was also strongly associated with tumor size,tumor depth,lymph node metastasis,distant metastasis,and tumor-node-metastasis stage.CONCLUSION Our findings revealed that the circulating exosomal miR-17-92 cluster may be used as a potential noninvasive biomarker to improve diagnostic efficiency for GC.展开更多
文摘目的探讨缺氧缺血性脑病新生儿血清miR-27a、miR-17-5p、miR-181a表达水平及其临床意义。方法选取2018年3月至2024年3月石家庄市妇幼保健院收治的184例缺氧缺血性脑病新生儿为研究组,随访6个月后,根据随访结果将其分为预后良好组和预后不良组;另选取同期在石家庄市妇幼保健院分娩的205例健康新生儿作为对照组。采用实时荧光定量PCR(qRT-PCR)测定各组血清miR-27a、miR-17-5p、miR-181a的相对表达水平。绘制受试者工作特征(ROC)曲线分析血清miR-27a、miR-17-5p、miR-181a单独及3项联合对缺氧缺血性脑病新生儿预后不良的预测价值。采用多因素Logistic回归分析缺氧缺血性脑病新生儿预后不良的影响因素。结果与对照组相比,研究组血清miR-27a、miR-17-5p表达水平降低,miR-181a表达水平升高,差异均有统计学意义(P<0.05)。6个月随访结果显示,预后良好组有128例,预后不良组有56例;与预后良好组相比,预后不良组血清miR-27a、miR-17-5p表达水平降低,miR-181a表达水平、宫内窘迫占比、1 min Apgar评分<4分占比升高,差异均有统计学意义(P<0.05)。ROC曲线分析结果显示,血清miR-27a、miR-17-5p、miR-181a联合预测缺氧缺血性脑病新生儿预后不良的曲线下面积(AUC)大于miR-27a、miR-17-5p、miR-181a单独预测的AUC(Z=3.041、3.587、2.907,均P<0.05)。多因素Logistic回归分析结果显示,宫内窘迫、1 min Apgar评分及miR-181a、miR-27a、miR-17-5p表达水平均为缺氧缺血性脑病新生儿预后不良的影响因素(P<0.05)。结论缺氧缺血性脑病新生儿血清miR-27a、miR-17-5p呈低表达,miR-181a呈高表达,且均为预后不良的影响因素,三者联合检测能提高缺氧缺血新生儿预后不良的预测效能。
基金Supported by National Natural Science Foundation of China,No.81902805Jiangsu Provincial Natural Science Foundation,No.BK20190174+1 种基金Suzhou Gusu Health Talent Research Project,No.GSWS2023039Suzhou Medical Youth Talents Project,No.Qngg2024004.
文摘BACKGROUND Gastric cancer(GC)is among the most common malignant tumors and remains a leading cause of cancer-related mortality worldwide.Furthermore,exosomal miRNAs are regarded as promising noninvasive biomarkers for diagnosing malignant tumors.AIM To investigate the expression of exosomal miR-17-92 clusters and develop a potential biomarker for GC diagnosis METHODS Exosomes were isolated from serum samples obtained from 72 GC patients and 20 healthy controls.The isolated exosomes were validated using transmission electron microscopy,nanoparticle tracking analysis,and western blotting.Exosomal RNA was then extracted,and the expression profile of the miR-17-92 cluster was analyzed using qRT-PCR.Statistical methods were employed to evaluate the relationship between the serum exosomal miR-17-92 cluster expre-ssion and the clinicopathological parameters of GC patients as well as to assess the diagnostic utility of these miRNAs.RESULTS The expression of four members of the exosomal miR-17-92 cluster-miR-17,miR-18,miR-19a,and miR-92-was significantly upregulated in the serum samples of patients with GC compared with those of healthy controls.The miR-17-92 cluster panel demonstrated substantially higher clinical diagnostic value for GC than any individual component or pair.Additionally,the expression of traditional tumor biomarkers-carcinoembryonic antigen and carbohydrate antigen 19-9-was significantly elevated in the serum of patients with GC compared with that of healthy controls.Each biomarker,whether alone or in combination,effectively differentiated the patients from healthy controls.Furthermore,a combined panel of the two traditional tumor biomarkers and the four miR-17-92 cluster members exhibited the highest diagnostic accuracy for GC.Elevated miR-17-92 expression was also strongly associated with tumor size,tumor depth,lymph node metastasis,distant metastasis,and tumor-node-metastasis stage.CONCLUSION Our findings revealed that the circulating exosomal miR-17-92 cluster may be used as a potential noninvasive biomarker to improve diagnostic efficiency for GC.
