Root-knot nematodes(RKNs)are the most widespread soil-borne obligate endoparasites.They can infect the roots of many crops and cause significant yield losses.The only commercially available RKN-resistant gene in tomat...Root-knot nematodes(RKNs)are the most widespread soil-borne obligate endoparasites.They can infect the roots of many crops and cause significant yield losses.The only commercially available RKN-resistant gene in tomatoes,Mi-1.2,fails at soil temperatures above 28℃.We cloned the heat-stable RKN-resistant gene,Mi-9,from a gene cluster composed of seven nucleotide-binding sites and leucine-rich repeat(NBS-LRR)type resistant genes in Solanum arcunum accession LA2157.Screening nematode infections in individual and combinatorial knockouts of five NBS-LRR genes showed that Mi-9 Candidate 4(MiC-4)alone is sufficient to confer heat-stable RKN resistance.Our study identifies a new source of heat-stable resistance to RKN in tomatoes for challenging environmental conditions.We also showcase a roadmap for rapid characterization of resistance genes by combining comparative genomics and genome editing,with the potential to be utilized in other crops.展开更多
基金supported by the National Key R&D Program of China(2018YFA0900600 and 2021YFF1000103-5)the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24030503)。
文摘Root-knot nematodes(RKNs)are the most widespread soil-borne obligate endoparasites.They can infect the roots of many crops and cause significant yield losses.The only commercially available RKN-resistant gene in tomatoes,Mi-1.2,fails at soil temperatures above 28℃.We cloned the heat-stable RKN-resistant gene,Mi-9,from a gene cluster composed of seven nucleotide-binding sites and leucine-rich repeat(NBS-LRR)type resistant genes in Solanum arcunum accession LA2157.Screening nematode infections in individual and combinatorial knockouts of five NBS-LRR genes showed that Mi-9 Candidate 4(MiC-4)alone is sufficient to confer heat-stable RKN resistance.Our study identifies a new source of heat-stable resistance to RKN in tomatoes for challenging environmental conditions.We also showcase a roadmap for rapid characterization of resistance genes by combining comparative genomics and genome editing,with the potential to be utilized in other crops.
