Methylotrophic yeasts and bacteria, which can use methanol as carbon and energy source, have beenwildly used as microbial cell factories for biomanufacturing. Due to their robustness in industrial harshconditions, met...Methylotrophic yeasts and bacteria, which can use methanol as carbon and energy source, have beenwildly used as microbial cell factories for biomanufacturing. Due to their robustness in industrial harshconditions, methylotrophic yeasts such as Pichia pastoris have been explored as a cell factory forproduction of proteins and high-value chemicals. Methanol utilization pathway （MUT） is highlyregulated for efficient methanol utilization, and the downstream pathways need extensively constructedand optimized toward target metabolite biosynthesis. Here, we present an overview of methanolmetabolism and regulation in methylotrophic yeasts, among which we focus on the regulation of keygenes involved in methanol metabolism. Besides, the recent progresses in construction and optimizationof downstream biosynthetic pathways for production of high value chemicals, such as polyketides, fattyacids and isoprenoids, are further summarized. Finally, we discuss the current challenges and feasiblestrategies toward constructing efficient methylotrophic cell factories may promote wide applications inthe future.展开更多
CSSC Power and Shanghai CSSC Mitsui Shipbuilding Diesel Engine jointly held CPGC/CMD-MAN B&W 12G95ME-C10.5-LGIM-EGRTC methanol Engine FAT handover ceremony,the latter is a subsidiary of MAN Energy Solutions(now re...CSSC Power and Shanghai CSSC Mitsui Shipbuilding Diesel Engine jointly held CPGC/CMD-MAN B&W 12G95ME-C10.5-LGIM-EGRTC methanol Engine FAT handover ceremony,the latter is a subsidiary of MAN Energy Solutions(now renamed Everllence)at CSSCPower.Based on MAN-ES latest generation LGIM design platform and control logics,the engine reliability and stability further improve;each engine is furnished with the exhaust recirculation emission system independently developed by Everllence,which is the largest dual-channel exhaust gas recirculation system for the two-stroke engine.展开更多
基金funded by the Young Investigator Grant from Dalian Institute of Chemicals Physics,Chinese Academy of Sciences(to Y.J.Zhou)
文摘Methylotrophic yeasts and bacteria, which can use methanol as carbon and energy source, have beenwildly used as microbial cell factories for biomanufacturing. Due to their robustness in industrial harshconditions, methylotrophic yeasts such as Pichia pastoris have been explored as a cell factory forproduction of proteins and high-value chemicals. Methanol utilization pathway （MUT） is highlyregulated for efficient methanol utilization, and the downstream pathways need extensively constructedand optimized toward target metabolite biosynthesis. Here, we present an overview of methanolmetabolism and regulation in methylotrophic yeasts, among which we focus on the regulation of keygenes involved in methanol metabolism. Besides, the recent progresses in construction and optimizationof downstream biosynthetic pathways for production of high value chemicals, such as polyketides, fattyacids and isoprenoids, are further summarized. Finally, we discuss the current challenges and feasiblestrategies toward constructing efficient methylotrophic cell factories may promote wide applications inthe future.
文摘CSSC Power and Shanghai CSSC Mitsui Shipbuilding Diesel Engine jointly held CPGC/CMD-MAN B&W 12G95ME-C10.5-LGIM-EGRTC methanol Engine FAT handover ceremony,the latter is a subsidiary of MAN Energy Solutions(now renamed Everllence)at CSSCPower.Based on MAN-ES latest generation LGIM design platform and control logics,the engine reliability and stability further improve;each engine is furnished with the exhaust recirculation emission system independently developed by Everllence,which is the largest dual-channel exhaust gas recirculation system for the two-stroke engine.
