Ferroptosis in combination with immune therapy emerges as a promising approach for cancer therapy.Herein,dual-responsive metal-polyphenol coordinated nanomedicines were developed for pH/glutathione(GSH)-responsive syn...Ferroptosis in combination with immune therapy emerges as a promising approach for cancer therapy.Herein,dual-responsive metal-polyphenol coordinated nanomedicines were developed for pH/glutathione(GSH)-responsive synergistic ferroptosis and immunotherapy.Our innovative strategy involves the development of a manganese-polyphenol coordinated nanostructure,leveraging the biocompatibility of bovine serum albumin(BSA)as a template to encapsulate the anticancer drug sorafenib.The tumor microenvironment(pH/GSH)prompts the disassembly of MnO_(2)and epigallocatechin gallate(EGCG),thereby releases the anticancer payload.Concurrently,MnO_(2)acts to deplete intracellular GSH,which in turn suppresses glutathione peroxidase activity,leading to an accumulation of lipid peroxides with cell ferroptosis.Additionally,the release of Mn^(2+)ions bolster the cyclic guanosine monophosphlic acid(GMP)-adenosine monophosphlic acid(AMP)synthase-stimulator of interferon gene(cGAS-STING)pathway,which,in conjunction with the immunogenic cell death(ICD)effect induced by tumor cell apoptosis,significantly promotes dendritic cell(DC)maturation and enhances the presentation of tumor antigens.This successively ignites a robust innate and adaptive immune response.Both in vitro and in vivo experiments have demonstrated that the concurrent administration of ferroptosis-inducing and immune-stimulating therapies can significantly inhibit tumor growth.展开更多
Ferroustherapy has gained great attention for anti-cancer treatment in recent years. Enlightened by temperature-mediated Fenton reaction in industrial waste water removal, we designed a iron-based polyphenol-coordinat...Ferroustherapy has gained great attention for anti-cancer treatment in recent years. Enlightened by temperature-mediated Fenton reaction in industrial waste water removal, we designed a iron-based polyphenol-coordinated nanomedicines for mild hyperthermia-assisted anti-cancer ferroustherapy. In brief, Fe-GA@BSA nanoparticles was synthesized by self-assembly and sorafenib(SRF) was loaded into Fe-GA@BSA to establish Fe-GA@BSA-SRF nanomedicines. The result nanomedicines can induce ferroptosis in cancer cells by accelerating Fenton reaction. And the photothermal effect of Fe-GA@BSA-SRF was used for mild hyperthermia-assisted ferroustherapy. The nanomedicines performs good anti-cancer therapeutic efficacy by inducing the production of ROS and inhibiting glutathione peroxidase 4(GPX4) expression in vitro and in vivo. Besides, the broad absorption of Fe-GA@BSA-SRF in near infrared region endows it with photoacoustic imaging ability. This study provides ideas about rational design on iron-based nanoparticles for anti-cancer ferroustherapy.展开更多
Due to the hypoxic state of the tumor microenvironment(TME),photodynamic therapy(PDT)suffers from insufficient ROS production.The metal-polyphenol network-mediated Fenton reaction can generate reac-tive oxygen species...Due to the hypoxic state of the tumor microenvironment(TME),photodynamic therapy(PDT)suffers from insufficient ROS production.The metal-polyphenol network-mediated Fenton reaction can generate reac-tive oxygen species(ROS)by consuming H_(2)O_(2) in TME,improving the inadequate ROS generation problem of PDT.Therefore,synergistic therapy combining PDT and Fenton response-based CDT is a promising ap-proach for cancer treatment.Herein,a metal-polyphenol nanocomposite was deposited with gallic acid grafted hyaluronic acid and Fe^(3+) to contrast a Ce6 nano-delivery system(Ce6@HSF NPs)for melanoma synergistic therapy.Ce6@HSF NPs could be used as a Fenton reagent to induce the·OH production and enhance the PDT effect of Ce6 to a certain extent.After 4 h of cellular uptake,the fluorescence intensity of Ce6 in the Ce6@HSF NPs group was higher than 3 times that in the Ce6 group.The intracellular ROS generation level of the Ce6@HSF NPs(L)group combining CDT and PDT was higher than that of the Ce6 group and Ce6@HSF NPs group.In vitro and in vivo anti-melanoma studies show that Ce6@HSF NPs(L)group exhibited better anti-melanoma than other groups.Together,Ce6@HSF NPs provide a promising synergistic treatment potential for melanoma.展开更多
本研究旨在探讨原花青素-铁网络涂层包裹乳酸菌后对发酵黑莓汁的影响。通过原花青素(Pc)与铁离子反应生成金属-多酚网络,将其包裹植物乳植杆菌BC17形成BC17@Pc-Fe保护结构,并应用于发酵黑莓汁中,研究原花青素-铁包裹乳酸菌后对黑莓汁发...本研究旨在探讨原花青素-铁网络涂层包裹乳酸菌后对发酵黑莓汁的影响。通过原花青素(Pc)与铁离子反应生成金属-多酚网络,将其包裹植物乳植杆菌BC17形成BC17@Pc-Fe保护结构,并应用于发酵黑莓汁中,研究原花青素-铁包裹乳酸菌后对黑莓汁发酵过程中的活菌数、总糖、还原糖、总酚、花色苷、pH、总酸以及抗氧化能力的影响。结果表明,包裹后的BC17的粒径由1990±156 nm增加至5560±901 nm,所带电荷由-13.77±1.44 mV增加至-4.05±1.48 mV;扫描电子显微镜研究显示,由原花青素和铁离子构成的金属-多酚网络成功包裹BC17。在发酵黑莓汁过程中,BC17@Pc-Fe组的活菌数能达到7.59±0.03 lg CFU/mL,显著(P<0.05)高于BC17组的活菌数(6.64±0.03 lg CFU/mL),其总糖和还原糖含量、总酚和花色苷含量与BC17组相比分别升高了13.29%±2.81%和6.49%±1.01%、4.47%±2.02%和33.96%±4.26%。同时,与BC17组相比,BC17@Pc-Fe组pH降低了2.72%±0.01%、总酸含量升高了4.60%±0.03%。经过抗氧化能力评估,BC17@Pc-Fe组的DPPH自由基清除率、ABTS+自由基清除率、·OH清除率以及亚铁离子还原能力比BC17组分别提高19.33%±2.94%、29.17%±2.82%、12.31%±7.22%和11.31%±0.03%,原花青素-铁网络涂层包裹BC17可以有效提高黑莓发酵汁的抗氧化能力。本研究通过改善乳酸菌生长和发酵性能来提升黑莓发酵汁的品质,为乳酸菌发酵果汁类产品提供了新思路。展开更多
用直接观察法、酒石酸亚铁法和铬黑 T 祛对绿茶茶汤组分与23种金属离子的络合性能进行了研究,结果表明,有22种金属离子可与茶汤组分发生络合或还原络合反应,其中有10种金属离子参与了茶多酚的络合。钙离子可与茶汤组分生成低溶解度的络...用直接观察法、酒石酸亚铁法和铬黑 T 祛对绿茶茶汤组分与23种金属离子的络合性能进行了研究,结果表明,有22种金属离子可与茶汤组分发生络合或还原络合反应,其中有10种金属离子参与了茶多酚的络合。钙离子可与茶汤组分生成低溶解度的络合物,其溶解度及稳定性可随反应溶液 pH 升高而下降。钙络合沉淀中的主要茶汤组分是茶多酚,其中以酯型儿茶素含量最高。展开更多
基金supported by the National Key R&D Program of China(No.2022YFC2304205)National Natural Science Foundation of China(Nos.51903062,52273128)the Plan on Enhancing Scientific Research in GMU(No.02-410-2405033).
文摘Ferroptosis in combination with immune therapy emerges as a promising approach for cancer therapy.Herein,dual-responsive metal-polyphenol coordinated nanomedicines were developed for pH/glutathione(GSH)-responsive synergistic ferroptosis and immunotherapy.Our innovative strategy involves the development of a manganese-polyphenol coordinated nanostructure,leveraging the biocompatibility of bovine serum albumin(BSA)as a template to encapsulate the anticancer drug sorafenib.The tumor microenvironment(pH/GSH)prompts the disassembly of MnO_(2)and epigallocatechin gallate(EGCG),thereby releases the anticancer payload.Concurrently,MnO_(2)acts to deplete intracellular GSH,which in turn suppresses glutathione peroxidase activity,leading to an accumulation of lipid peroxides with cell ferroptosis.Additionally,the release of Mn^(2+)ions bolster the cyclic guanosine monophosphlic acid(GMP)-adenosine monophosphlic acid(AMP)synthase-stimulator of interferon gene(cGAS-STING)pathway,which,in conjunction with the immunogenic cell death(ICD)effect induced by tumor cell apoptosis,significantly promotes dendritic cell(DC)maturation and enhances the presentation of tumor antigens.This successively ignites a robust innate and adaptive immune response.Both in vitro and in vivo experiments have demonstrated that the concurrent administration of ferroptosis-inducing and immune-stimulating therapies can significantly inhibit tumor growth.
基金supported by grants from the National Natural Science Foundation of China (No. 51903062)Guangdong Basic and Applied Basic Research Foundation (No.2020A1515011320)+1 种基金Science and Technology Projects of Guangzhou(No. 202102020757)Subject Construction Project of Basic Medical Sciences of Guangzhou Medical University (Nos.JCXKJS2021B07, JCXKJS2021D09)。
文摘Ferroustherapy has gained great attention for anti-cancer treatment in recent years. Enlightened by temperature-mediated Fenton reaction in industrial waste water removal, we designed a iron-based polyphenol-coordinated nanomedicines for mild hyperthermia-assisted anti-cancer ferroustherapy. In brief, Fe-GA@BSA nanoparticles was synthesized by self-assembly and sorafenib(SRF) was loaded into Fe-GA@BSA to establish Fe-GA@BSA-SRF nanomedicines. The result nanomedicines can induce ferroptosis in cancer cells by accelerating Fenton reaction. And the photothermal effect of Fe-GA@BSA-SRF was used for mild hyperthermia-assisted ferroustherapy. The nanomedicines performs good anti-cancer therapeutic efficacy by inducing the production of ROS and inhibiting glutathione peroxidase 4(GPX4) expression in vitro and in vivo. Besides, the broad absorption of Fe-GA@BSA-SRF in near infrared region endows it with photoacoustic imaging ability. This study provides ideas about rational design on iron-based nanoparticles for anti-cancer ferroustherapy.
基金This work was financially supported by the National Natural Sciences Foundation of China(Nos.31971308,81960769 and U1903211)the National S&T Major Project(No.2019ZX09301-147)+1 种基金the Sichuan Science and Technology Program(No.2022YFS0007)the Luzhou Science and Technology Plan(No.2018CDLZ-10).
文摘Due to the hypoxic state of the tumor microenvironment(TME),photodynamic therapy(PDT)suffers from insufficient ROS production.The metal-polyphenol network-mediated Fenton reaction can generate reac-tive oxygen species(ROS)by consuming H_(2)O_(2) in TME,improving the inadequate ROS generation problem of PDT.Therefore,synergistic therapy combining PDT and Fenton response-based CDT is a promising ap-proach for cancer treatment.Herein,a metal-polyphenol nanocomposite was deposited with gallic acid grafted hyaluronic acid and Fe^(3+) to contrast a Ce6 nano-delivery system(Ce6@HSF NPs)for melanoma synergistic therapy.Ce6@HSF NPs could be used as a Fenton reagent to induce the·OH production and enhance the PDT effect of Ce6 to a certain extent.After 4 h of cellular uptake,the fluorescence intensity of Ce6 in the Ce6@HSF NPs group was higher than 3 times that in the Ce6 group.The intracellular ROS generation level of the Ce6@HSF NPs(L)group combining CDT and PDT was higher than that of the Ce6 group and Ce6@HSF NPs group.In vitro and in vivo anti-melanoma studies show that Ce6@HSF NPs(L)group exhibited better anti-melanoma than other groups.Together,Ce6@HSF NPs provide a promising synergistic treatment potential for melanoma.
文摘本研究旨在探讨原花青素-铁网络涂层包裹乳酸菌后对发酵黑莓汁的影响。通过原花青素(Pc)与铁离子反应生成金属-多酚网络,将其包裹植物乳植杆菌BC17形成BC17@Pc-Fe保护结构,并应用于发酵黑莓汁中,研究原花青素-铁包裹乳酸菌后对黑莓汁发酵过程中的活菌数、总糖、还原糖、总酚、花色苷、pH、总酸以及抗氧化能力的影响。结果表明,包裹后的BC17的粒径由1990±156 nm增加至5560±901 nm,所带电荷由-13.77±1.44 mV增加至-4.05±1.48 mV;扫描电子显微镜研究显示,由原花青素和铁离子构成的金属-多酚网络成功包裹BC17。在发酵黑莓汁过程中,BC17@Pc-Fe组的活菌数能达到7.59±0.03 lg CFU/mL,显著(P<0.05)高于BC17组的活菌数(6.64±0.03 lg CFU/mL),其总糖和还原糖含量、总酚和花色苷含量与BC17组相比分别升高了13.29%±2.81%和6.49%±1.01%、4.47%±2.02%和33.96%±4.26%。同时,与BC17组相比,BC17@Pc-Fe组pH降低了2.72%±0.01%、总酸含量升高了4.60%±0.03%。经过抗氧化能力评估,BC17@Pc-Fe组的DPPH自由基清除率、ABTS+自由基清除率、·OH清除率以及亚铁离子还原能力比BC17组分别提高19.33%±2.94%、29.17%±2.82%、12.31%±7.22%和11.31%±0.03%,原花青素-铁网络涂层包裹BC17可以有效提高黑莓发酵汁的抗氧化能力。本研究通过改善乳酸菌生长和发酵性能来提升黑莓发酵汁的品质,为乳酸菌发酵果汁类产品提供了新思路。
文摘用直接观察法、酒石酸亚铁法和铬黑 T 祛对绿茶茶汤组分与23种金属离子的络合性能进行了研究,结果表明,有22种金属离子可与茶汤组分发生络合或还原络合反应,其中有10种金属离子参与了茶多酚的络合。钙离子可与茶汤组分生成低溶解度的络合物,其溶解度及稳定性可随反应溶液 pH 升高而下降。钙络合沉淀中的主要茶汤组分是茶多酚,其中以酯型儿茶素含量最高。
