Objective:To study the incidence of encysted progenetic metacercariae of Clinostomum complanatum(C. complanatum) in Channa punctatus(C. punctatus), associated histopathology and the experimental infection to laborator...Objective:To study the incidence of encysted progenetic metacercariae of Clinostomum complanatum(C. complanatum) in Channa punctatus(C. punctatus), associated histopathology and the experimental infection to laboratory chicken to obtain ovigerous adult worms.Methods:Live C. punctatus were brought from local fish market of Aligarh, India, dissected and examined on a monthly basis for the presence of C. complanatumcysts. For histochemistry, infected tissue sections with attached cysts were processed for haematoxylene and eosin staining. Cysts were aseptically fed to 4 day old leghorn chicken to obtain adult worms. Mechanically excysted metacercaria and the ovigerous adult worms were stained in carmine to prepare permanent slides.Results:One year survey for the infection of encysted progenetic metacercaria of C.complanatuminC. punctatusrevealed the prevalence, intensity and abundance of 24.7%, 2.27and 0.608, respectively. Histopathology showed heavy infiltration of immune cells at the site of cyst attachment and some tissue damage was also evident. Following feeding to experimental chicken, about 41.07% of the encysted metacercariae were able to excyst and migrate back to bucco-pharyngeal region where they tenaciously attached and fed on blood, and transformed into ovigerous adult worms from 62 hours onwards of post infection.Conclusions:The parasite is potentially pathogenic to the host, and the availability of a suitable intermediate host can be a contributing factor for the occurrence of C. complanatummetacercaria either in the excysted or encysted form, indicating loose host specificity and zoonotic potential.展开更多
基金financially supported by Aligarh Muslim University(grant No.09PHDL128)
文摘Objective:To study the incidence of encysted progenetic metacercariae of Clinostomum complanatum(C. complanatum) in Channa punctatus(C. punctatus), associated histopathology and the experimental infection to laboratory chicken to obtain ovigerous adult worms.Methods:Live C. punctatus were brought from local fish market of Aligarh, India, dissected and examined on a monthly basis for the presence of C. complanatumcysts. For histochemistry, infected tissue sections with attached cysts were processed for haematoxylene and eosin staining. Cysts were aseptically fed to 4 day old leghorn chicken to obtain adult worms. Mechanically excysted metacercaria and the ovigerous adult worms were stained in carmine to prepare permanent slides.Results:One year survey for the infection of encysted progenetic metacercaria of C.complanatuminC. punctatusrevealed the prevalence, intensity and abundance of 24.7%, 2.27and 0.608, respectively. Histopathology showed heavy infiltration of immune cells at the site of cyst attachment and some tissue damage was also evident. Following feeding to experimental chicken, about 41.07% of the encysted metacercariae were able to excyst and migrate back to bucco-pharyngeal region where they tenaciously attached and fed on blood, and transformed into ovigerous adult worms from 62 hours onwards of post infection.Conclusions:The parasite is potentially pathogenic to the host, and the availability of a suitable intermediate host can be a contributing factor for the occurrence of C. complanatummetacercaria either in the excysted or encysted form, indicating loose host specificity and zoonotic potential.
