[Objective] The research aimed to lay a foundation for the screening of cell lines producing secondary metabolites of Brucea javanica(L.)Merr.[Method] The insecticidal activities of the extracts from branch and 3 diff...[Objective] The research aimed to lay a foundation for the screening of cell lines producing secondary metabolites of Brucea javanica(L.)Merr.[Method] The insecticidal activities of the extracts from branch and 3 different types of calluses of Brucea javanica(L.)Merr.was detected through methods of leaf disc and potted seedlings against the diamond back moth.[Result] Extracts from four kinds of Brucea javanica(L.)Merr.tissues assumed both the activities of antifeedant and oviposition deterrency against the diamond back moth.Antifeedant effect of extracts was in turn the callus C< callus B< callus A< branches.Oviposition deterrency activity of the extracts was in turn the callus A> branch > callus B>callus C.The insecticidal activities of callus A and B were higher than that of the callus C.[Conclusion] The results show that insecticidal activity of callus and its growth rate is inversely proportional.展开更多
目的为探知天冬Asparagus cochinchinensis块根生长膨大的分子机制,对不同膨大期的天冬进行转录组测序,挖掘促使块根生长膨大的关键基因。方法以不同膨大期的天冬为材料,采用Illumina HiSeq高通量测序平台进行转录组测序,组装与注释后...目的为探知天冬Asparagus cochinchinensis块根生长膨大的分子机制,对不同膨大期的天冬进行转录组测序,挖掘促使块根生长膨大的关键基因。方法以不同膨大期的天冬为材料,采用Illumina HiSeq高通量测序平台进行转录组测序,组装与注释后进行差异表达基因(differential gene,DEGs)筛选。结果转录组测序共获得59.20 Gb clean data,Q30均不小于94.23%,共得到99930条Unigene,其中47857条被注释。3种不同膨大状态的天冬中的DEGs有1429个,GO富集结果表明,3种不同膨大期天冬的DEGs主要富集于细胞过程、代谢过程、结合力与催化活性等功能,同源蛋白簇(clusters of orthologous groups,COG)富集结果表明,3种不同膨大期的天冬中DEGs主要富集于信号转导机制、碳水化合物转运代谢以及细胞壁/膜/被膜的生物合成。京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析显示,与根膨大相关的通路[植物激素信号传导、丝裂原活化蛋白激酶(mitogen-activated protein kinases,MAPK)信号通路、淀粉与蔗糖代谢以及苯丙烷生物合成]为DEGs代表性富集通路。结合差异基因的注释信息以及表达量情况分析表明,多种酶类(SAUR、XTH、LRR-RLPK、SPP、EG、BGLU、TPP、AMY、BAMY、PAL、PER)参与天冬块根膨大,其中XTH可能是影响天冬块根的膨大发育过程的最主要的酶类。结论通过对不同膨大状态天冬的高通量转录组测序,揭示了天冬块根生长膨大的关键酶基因,可为天冬根系生长膨大的生物学机制提供参考。展开更多
基金Supported by Guangxi Science Foundation(0639027)Science and Technology Developmental Fund of Guangxi Academy of Agricultural Sciences(2006025)~~
文摘[Objective] The research aimed to lay a foundation for the screening of cell lines producing secondary metabolites of Brucea javanica(L.)Merr.[Method] The insecticidal activities of the extracts from branch and 3 different types of calluses of Brucea javanica(L.)Merr.was detected through methods of leaf disc and potted seedlings against the diamond back moth.[Result] Extracts from four kinds of Brucea javanica(L.)Merr.tissues assumed both the activities of antifeedant and oviposition deterrency against the diamond back moth.Antifeedant effect of extracts was in turn the callus C< callus B< callus A< branches.Oviposition deterrency activity of the extracts was in turn the callus A> branch > callus B>callus C.The insecticidal activities of callus A and B were higher than that of the callus C.[Conclusion] The results show that insecticidal activity of callus and its growth rate is inversely proportional.
文摘目的为探知天冬Asparagus cochinchinensis块根生长膨大的分子机制,对不同膨大期的天冬进行转录组测序,挖掘促使块根生长膨大的关键基因。方法以不同膨大期的天冬为材料,采用Illumina HiSeq高通量测序平台进行转录组测序,组装与注释后进行差异表达基因(differential gene,DEGs)筛选。结果转录组测序共获得59.20 Gb clean data,Q30均不小于94.23%,共得到99930条Unigene,其中47857条被注释。3种不同膨大状态的天冬中的DEGs有1429个,GO富集结果表明,3种不同膨大期天冬的DEGs主要富集于细胞过程、代谢过程、结合力与催化活性等功能,同源蛋白簇(clusters of orthologous groups,COG)富集结果表明,3种不同膨大期的天冬中DEGs主要富集于信号转导机制、碳水化合物转运代谢以及细胞壁/膜/被膜的生物合成。京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析显示,与根膨大相关的通路[植物激素信号传导、丝裂原活化蛋白激酶(mitogen-activated protein kinases,MAPK)信号通路、淀粉与蔗糖代谢以及苯丙烷生物合成]为DEGs代表性富集通路。结合差异基因的注释信息以及表达量情况分析表明,多种酶类(SAUR、XTH、LRR-RLPK、SPP、EG、BGLU、TPP、AMY、BAMY、PAL、PER)参与天冬块根膨大,其中XTH可能是影响天冬块根的膨大发育过程的最主要的酶类。结论通过对不同膨大状态天冬的高通量转录组测序,揭示了天冬块根生长膨大的关键酶基因,可为天冬根系生长膨大的生物学机制提供参考。
