The presence of toxic mercury (Ⅱ) in water is an ever-growing problem on earth that has various harmful effect on human health and aquatic living organisms.Therefore,detection of mercury (Ⅱ) in water is very much cr...The presence of toxic mercury (Ⅱ) in water is an ever-growing problem on earth that has various harmful effect on human health and aquatic living organisms.Therefore,detection of mercury (Ⅱ) in water is very much crucial and several researches are going on in this topic.Metal-organic frameworks (MOFs) are considered as an effective device for sensing of toxic heavy metal ions in water.The tunable functionalities with large surface area of highly semiconducting MOFs enhance its activity towards fluorescence sensing.In this study,we are reporting one highly selective and sensitive luminescent sensor for the detection of mercury (Ⅱ) in water.A series of binary MOF composites were synthesized using in-situ solvothermal synthetic technique for fluorescence sensing of Hg^(2+)in water.The welldistributed graphitic carbon nitride quantum dots on porous zirconium-based MOF improve Hg^(2+)sensing activity in water owing to their great electronic and optical properties.The binary MOF composite (2) i.e.,the sensor exhibited excellent limit of detection (LOD) value of 2.4 nmol/L for Hg^(2+).The sensor also exhibited excellent performance for mercury (Ⅱ)detection in real water samples.The characterizations of the synthesized materials were done using various spectroscopic techniques and the fluorescence sensing mechanism was studied.展开更多
Concerns over exposure to mercury have motivated the exploration of cost-effective,rapid,and reliable method for monitoring Hg^2＋ in the environment.Recently,surface-enhanced Raman scattering（SERS） has become a pro...Concerns over exposure to mercury have motivated the exploration of cost-effective,rapid,and reliable method for monitoring Hg^2＋ in the environment.Recently,surface-enhanced Raman scattering（SERS） has become a promising alternative method for Hg^2＋ analysis.SERS is a spectroscopic technique which combines modern laser spectroscopy with the optical properties of nano-sized noble metal structures,resulting in substantially increased Raman signals.When Hg^2＋ is in a close contact with metallic nanostructures,the SERS effect provides unique structural information together with ultrasensitive detection limits.This review introduces the principles and contemporary approaches of SERS-based Hg^2＋detection.In addition,the perspective and challenges are briefly discussed.展开更多
Two homochiral mercury （1） and cadmium （2） complexes derived from chiral twisted biphenyl pyridine ligand （3,3＇-bipyridine-5,5＇,6,6＇-tetramethyl-2,2＇-dimethoxy-1,1＇-biphenyl） have been synthesized and chara...Two homochiral mercury （1） and cadmium （2） complexes derived from chiral twisted biphenyl pyridine ligand （3,3＇-bipyridine-5,5＇,6,6＇-tetramethyl-2,2＇-dimethoxy-1,1＇-biphenyl） have been synthesized and characterized by IR, microanalysis, TGA, UV-Vis, powder and single-crystal X-ray crystallography. Both 1 and 2 crystallize in orthorhombic space group P21212 with Z = 2. For 1, a = 14.2038（16）, b = 14.3630（17）, c = 7.0257（8）, V = 1433.3（3）3, Mr = 878.91, Dc = 2.037 g/cm3, μ = 7.549 mm-1, F（000） = 824, the final GOF = 1.017, R = 0.0296 and wR = 0.0645 for 2925 observed reflections with I 2σ（I）. For 2, a = 14.212（3）, b = 14.392（3）, c = 7.0498（14）, V = 1442.0（5）3, Mr = 790.72, Dc = 1.821 g/cm3, μ = 2.924 mm–1, F（000） = 760, the final GOF = 1.075, R = 0.0340 and wR = 0.0834 for 3144 observed reflections with I 2σ（I）. The crystal structures of 1 and 2 are isostructural and each adopts a two-dimensional supramolecular network which contains the C–H···π interactions.展开更多
CdSe/CdS quantum dots （QDs） functionalized by thiourea （TU） were synthesized and used as a fluorescent sensor for mercury ion detection. The TU-functionalized QDs were prepared by bonding TU via electrostatic inte...CdSe/CdS quantum dots （QDs） functionalized by thiourea （TU） were synthesized and used as a fluorescent sensor for mercury ion detection. The TU-functionalized QDs were prepared by bonding TU via electrostatic interaction to the core/shell CdSe/CdS QDs after capping with thioglycolic acid （TGA）. It was observed that the fluorescence of the functionalized QDs was quenched upon the addition of Hg^2＋. The quantitative detection of Hg^2＋ with this fluorescent sensor could be conducted based on the linear relationship between the extent of quenching and the concentration of Hg^2＋ added in the range of 1-300 μg.L^-1, A detection limit of 0.56 μg.L^-1 was achieved. The sensor showed superior selectivity for Hg^2＋ and was successfully applied to the determination of mercury in environmental samples with satisfactory results展开更多
Mercury ions have been considered highly toxic to human health. What would be great is to develop the ionic probes without any toxicities themselves. Here, we report a friendly, highly sensitive mercury(II) ionic prob...Mercury ions have been considered highly toxic to human health. What would be great is to develop the ionic probes without any toxicities themselves. Here, we report a friendly, highly sensitive mercury(II) ionic probe, watersoluble photoluminescence carbon dots which were synthesized by simply hydrothermal treatment of fresh cherry tomatoes without adding any other reagents. The ultra-small(\1 nm) carbon dots show robust excitation-depended photoluminescence under a wide p H range(4–10) or a strong ionic strength of up to 1 M, and the detection limit of mercury(II) has been determined as low as 18 n M. We envision such water-soluble, biocompatible carbon dots that could be applied to biolabeling, bio-imaging, and biosensing fields.展开更多
An Eastman-AQ/Ni(II)chemically modified electrode(CME) produced by“double coating steps”deposition of Eastman-AQ/Ni(II) film and Ni(II)-coating crystalline species onto glassy carbon instead of metallic nickel elect...An Eastman-AQ/Ni(II)chemically modified electrode(CME) produced by“double coating steps”deposition of Eastman-AQ/Ni(II) film and Ni(II)-coating crystalline species onto glassy carbon instead of metallic nickel electrode exhibited stable electrocatalytic oxidation of numerous ∝-hydrogen compounds including carbohydrates, amines and amino acids.Determination of glucose in FIA on the CME, the linear response concentration range was bstween 1.0×10^(-5) and 5.0×10^(-2) mol/L and the detection limit was 5.0×10^(-6)mol/L.The stability of the CME was adequate for routine quantitative application.展开更多
Objective: The objective of the study is to verify the clinical validity of the following kits with the comparative experimental analysis and evaluate whether their performance can meet the clinical requirements, i.e....Objective: The objective of the study is to verify the clinical validity of the following kits with the comparative experimental analysis and evaluate whether their performance can meet the clinical requirements, i.e. Class III in vitro diagnostic reagent “Herpes Simplex Virus (HSV) Type II Nucleic Acid Detection Kit (PCR-Fluorescence Probe Method)” of Daan Gene Co., Ltd. (Daan kit for short) and “Herpes Simplex Virus (HSV) Type II Nucleic Acid Detection Kit (Fluorescence PCR Method)” of Wuhan Biot Gene Co., Ltd. (Biot kit for short). Method: In the study process, the samples were divided into positive and negative groups according to the control test results, and the clinical application performance of Daan kit and Biot kit was evaluated by comparing their test results. Results: The results show that two kits indicate the same test results, i.e. 26 positive and 107 negative samples in a total of 133 male urethral discharge samples, and 32 positive and 238 negative samples in a total of 270 female cervical secretion samples. Conclusion: It can be concluded from the clinical test that Daan and Biot Herpes Simplex Virus (HSV) Type II Nuc- leic Acid Test Kits are reliable, accurate, safe, convenient for use, stable and high-value in the clinical application.展开更多
Mercury(Hg^(2+))has been recognized as a global pollutant with a toxic,mobile,and persistent nature.It adversely affects the ecosystem and human health.Already developed biosensors for Hg^(2+)detection majorly suffer ...Mercury(Hg^(2+))has been recognized as a global pollutant with a toxic,mobile,and persistent nature.It adversely affects the ecosystem and human health.Already developed biosensors for Hg^(2+)detection majorly suffer from poor sensitivity and specificity.Herein,a colorimetric/fluorimetric dual-mode sensing approach is designed for the quantitative detection of Hg^(2+).This novel sensing approach utilizes nanofluorophores,i.e.,fluorescent copper nanoclusters-doped zirconia metal-organic framework(CuNCs@Zr-MOF)nanoconjugate(blue color)and N-methyl mesoporphyrin IX(NMM)(red color)in combination with peroxidase-mimicking G-quadruplex DNAzyme(PMDNAzyme).In the presence of Hg^(2+),dabcyl conjugated complementary DNA with T-T mismatches form the stable duplex with the CuNCs@Zr-MOF@G-quadruplex structure through T-Hg^(2+)-T base pairing.It causes the quenching of fluorescence of CuNCs@Zr-MOF(463 nm)due to the Förster resonance energy transfer(FRET)system.Moreover,the G-quadruplex(G4)structure of the aptamer enhances the fluorescence emission of NMM(610 nm).Besides this,the peroxidase-like activity of G4/hemin DNAzyme offers the colorimetric detection of Hg^(2+).The formation of duplex with PMDNAzyme increases the catalytic activity.This novel biosensing probe quantitatively detected Hg^(2+)using both fluorimetry and colorimetry approaches with a low detection limit of 0.59 and 36.3 nM,respectively.It was also observed that the presence of interfering metal ions in case of real aqueous samples does not affect the performance of this novel biosensing probe.These findings confirm the considerable potential of the proposed biosensing probe to screen the concentration of Hg^(2+)in aquatic products.展开更多
文摘The presence of toxic mercury (Ⅱ) in water is an ever-growing problem on earth that has various harmful effect on human health and aquatic living organisms.Therefore,detection of mercury (Ⅱ) in water is very much crucial and several researches are going on in this topic.Metal-organic frameworks (MOFs) are considered as an effective device for sensing of toxic heavy metal ions in water.The tunable functionalities with large surface area of highly semiconducting MOFs enhance its activity towards fluorescence sensing.In this study,we are reporting one highly selective and sensitive luminescent sensor for the detection of mercury (Ⅱ) in water.A series of binary MOF composites were synthesized using in-situ solvothermal synthetic technique for fluorescence sensing of Hg^(2+)in water.The welldistributed graphitic carbon nitride quantum dots on porous zirconium-based MOF improve Hg^(2+)sensing activity in water owing to their great electronic and optical properties.The binary MOF composite (2) i.e.,the sensor exhibited excellent limit of detection (LOD) value of 2.4 nmol/L for Hg^(2+).The sensor also exhibited excellent performance for mercury (Ⅱ)detection in real water samples.The characterizations of the synthesized materials were done using various spectroscopic techniques and the fluorescence sensing mechanism was studied.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDB14020201)the National Natural Science Foundation of China (Nos. 21337004, 21321004)
文摘Concerns over exposure to mercury have motivated the exploration of cost-effective,rapid,and reliable method for monitoring Hg^2＋ in the environment.Recently,surface-enhanced Raman scattering（SERS） has become a promising alternative method for Hg^2＋ analysis.SERS is a spectroscopic technique which combines modern laser spectroscopy with the optical properties of nano-sized noble metal structures,resulting in substantially increased Raman signals.When Hg^2＋ is in a close contact with metallic nanostructures,the SERS effect provides unique structural information together with ultrasensitive detection limits.This review introduces the principles and contemporary approaches of SERS-based Hg^2＋detection.In addition,the perspective and challenges are briefly discussed.
基金supported by NNSFC (20971085)"973" Programs (2007CB209701 and 2009CB930403)the Key Project and Scientific Research Foundation for the Returned Overseas Chinese Scholars of State Education Ministry
文摘Two homochiral mercury （1） and cadmium （2） complexes derived from chiral twisted biphenyl pyridine ligand （3,3＇-bipyridine-5,5＇,6,6＇-tetramethyl-2,2＇-dimethoxy-1,1＇-biphenyl） have been synthesized and characterized by IR, microanalysis, TGA, UV-Vis, powder and single-crystal X-ray crystallography. Both 1 and 2 crystallize in orthorhombic space group P21212 with Z = 2. For 1, a = 14.2038（16）, b = 14.3630（17）, c = 7.0257（8）, V = 1433.3（3）3, Mr = 878.91, Dc = 2.037 g/cm3, μ = 7.549 mm-1, F（000） = 824, the final GOF = 1.017, R = 0.0296 and wR = 0.0645 for 2925 observed reflections with I 2σ（I）. For 2, a = 14.212（3）, b = 14.392（3）, c = 7.0498（14）, V = 1442.0（5）3, Mr = 790.72, Dc = 1.821 g/cm3, μ = 2.924 mm–1, F（000） = 760, the final GOF = 1.075, R = 0.0340 and wR = 0.0834 for 3144 observed reflections with I 2σ（I）. The crystal structures of 1 and 2 are isostructural and each adopts a two-dimensional supramolecular network which contains the C–H···π interactions.
基金the financial support from the National Natural Science Foundation of China (Nos. 20345006 and 20575043)
文摘CdSe/CdS quantum dots （QDs） functionalized by thiourea （TU） were synthesized and used as a fluorescent sensor for mercury ion detection. The TU-functionalized QDs were prepared by bonding TU via electrostatic interaction to the core/shell CdSe/CdS QDs after capping with thioglycolic acid （TGA）. It was observed that the fluorescence of the functionalized QDs was quenched upon the addition of Hg^2＋. The quantitative detection of Hg^2＋ with this fluorescent sensor could be conducted based on the linear relationship between the extent of quenching and the concentration of Hg^2＋ added in the range of 1-300 μg.L^-1, A detection limit of 0.56 μg.L^-1 was achieved. The sensor showed superior selectivity for Hg^2＋ and was successfully applied to the determination of mercury in environmental samples with satisfactory results
基金supported by Grants from the National Natural Science Foundation of China (Nos. 21171086 and 81160213)Inner Mongolia Grassland Talent (No. 108-108038)+2 种基金Natural Science Foundation of Inner Mongolia Autonomous Region of China (Nos. 2013MS1121 and 2015MS0806)Inner Mongolia Department of Science and Technology (No. 211-202077)the Inner Mongolia Agricultural University (Nos. 109-108040, 211-109003, and 211-206038)
文摘Mercury ions have been considered highly toxic to human health. What would be great is to develop the ionic probes without any toxicities themselves. Here, we report a friendly, highly sensitive mercury(II) ionic probe, watersoluble photoluminescence carbon dots which were synthesized by simply hydrothermal treatment of fresh cherry tomatoes without adding any other reagents. The ultra-small(\1 nm) carbon dots show robust excitation-depended photoluminescence under a wide p H range(4–10) or a strong ionic strength of up to 1 M, and the detection limit of mercury(II) has been determined as low as 18 n M. We envision such water-soluble, biocompatible carbon dots that could be applied to biolabeling, bio-imaging, and biosensing fields.
文摘An Eastman-AQ/Ni(II)chemically modified electrode(CME) produced by“double coating steps”deposition of Eastman-AQ/Ni(II) film and Ni(II)-coating crystalline species onto glassy carbon instead of metallic nickel electrode exhibited stable electrocatalytic oxidation of numerous ∝-hydrogen compounds including carbohydrates, amines and amino acids.Determination of glucose in FIA on the CME, the linear response concentration range was bstween 1.0×10^(-5) and 5.0×10^(-2) mol/L and the detection limit was 5.0×10^(-6)mol/L.The stability of the CME was adequate for routine quantitative application.
文摘Objective: The objective of the study is to verify the clinical validity of the following kits with the comparative experimental analysis and evaluate whether their performance can meet the clinical requirements, i.e. Class III in vitro diagnostic reagent “Herpes Simplex Virus (HSV) Type II Nucleic Acid Detection Kit (PCR-Fluorescence Probe Method)” of Daan Gene Co., Ltd. (Daan kit for short) and “Herpes Simplex Virus (HSV) Type II Nucleic Acid Detection Kit (Fluorescence PCR Method)” of Wuhan Biot Gene Co., Ltd. (Biot kit for short). Method: In the study process, the samples were divided into positive and negative groups according to the control test results, and the clinical application performance of Daan kit and Biot kit was evaluated by comparing their test results. Results: The results show that two kits indicate the same test results, i.e. 26 positive and 107 negative samples in a total of 133 male urethral discharge samples, and 32 positive and 238 negative samples in a total of 270 female cervical secretion samples. Conclusion: It can be concluded from the clinical test that Daan and Biot Herpes Simplex Virus (HSV) Type II Nuc- leic Acid Test Kits are reliable, accurate, safe, convenient for use, stable and high-value in the clinical application.
基金Funding:S.K.thanks the Department of Biotechnology(DBT),Government of India,for research grant(award BT/PR18868/BCE/8/1370/2016 dated 2018 January 31)M.N.is grateful to CSIR for the SRA fellowship(no.B-12857,dated 2021 October 21).
文摘Mercury(Hg^(2+))has been recognized as a global pollutant with a toxic,mobile,and persistent nature.It adversely affects the ecosystem and human health.Already developed biosensors for Hg^(2+)detection majorly suffer from poor sensitivity and specificity.Herein,a colorimetric/fluorimetric dual-mode sensing approach is designed for the quantitative detection of Hg^(2+).This novel sensing approach utilizes nanofluorophores,i.e.,fluorescent copper nanoclusters-doped zirconia metal-organic framework(CuNCs@Zr-MOF)nanoconjugate(blue color)and N-methyl mesoporphyrin IX(NMM)(red color)in combination with peroxidase-mimicking G-quadruplex DNAzyme(PMDNAzyme).In the presence of Hg^(2+),dabcyl conjugated complementary DNA with T-T mismatches form the stable duplex with the CuNCs@Zr-MOF@G-quadruplex structure through T-Hg^(2+)-T base pairing.It causes the quenching of fluorescence of CuNCs@Zr-MOF(463 nm)due to the Förster resonance energy transfer(FRET)system.Moreover,the G-quadruplex(G4)structure of the aptamer enhances the fluorescence emission of NMM(610 nm).Besides this,the peroxidase-like activity of G4/hemin DNAzyme offers the colorimetric detection of Hg^(2+).The formation of duplex with PMDNAzyme increases the catalytic activity.This novel biosensing probe quantitatively detected Hg^(2+)using both fluorimetry and colorimetry approaches with a low detection limit of 0.59 and 36.3 nM,respectively.It was also observed that the presence of interfering metal ions in case of real aqueous samples does not affect the performance of this novel biosensing probe.These findings confirm the considerable potential of the proposed biosensing probe to screen the concentration of Hg^(2+)in aquatic products.
