Minthostachys mollis(Benth)Griseb.is a variety of‘peperina’with high pulegone+methone content but with a higher menthone content.The objective was to evaluate the antioxidant activity(AA)of peperina essential oil(PE...Minthostachys mollis(Benth)Griseb.is a variety of‘peperina’with high pulegone+methone content but with a higher menthone content.The objective was to evaluate the antioxidant activity(AA)of peperina essential oil(PEO)lower in pulegone/MENT(menthone+isomenthone)ratio combined with butylated hydroxytoluene(BHT).PEO was performed by gas chromatography with mass spectrometry(GC-MS).The thermal stability of PEO was determined at 60℃ for 28 days and analysed by GC-MS with SPME.Free Radical Scavening Activity(FRSA-DPPH test)and Total Phenol Content(TPC-analysed with Folin-cicolteau reagent)determined indirect AA.Direct AA was performed with accelerated oxidation of sunflower oil for 28 days at 60℃.Concentrations of 0.02,0.01 and 0.005%w/w(1,2 and 3)were used for the antioxidant BHT(B1,B2 and B3),PEO(P1,P2 and P3)and combinations.Chemical indicators(Conjugated Dienes,Peroxide Value and Anisidine Value),complete oxidation(Total Oxidation Value and Integral Oxidation Value)and volatiles(2-heptenal(Z)and 2,4-Decadienal(E,E))were determined.The pulegone/MENT ratio was 0.59 but the combinations B2P2 and B2P1 allow the use of lower concentration of BHT with higher efficiency.The low pulegone/MENT PEO can be combined with BHT to delay oxidation.展开更多
Objective: To investigate the chemical composition, in vitro antibacterial and antifungal activity of Mentha longifolia L. essential oils using aqueous extract. Methods: Mentha longifolia L. essential oils were extrac...Objective: To investigate the chemical composition, in vitro antibacterial and antifungal activity of Mentha longifolia L. essential oils using aqueous extract. Methods: Mentha longifolia L. essential oils were extracted using hydrodistillation with Clevenger apparatus for 3 h and the yield of the essential oil was calculated. Essential oils were qualitatively and quantitatively analyzed with gas chromatography and gas chromatography-mass spectrometer. For these essential oils, antibacterial and antifungal activity against human pathogens were evaluated. Results: Nineteen chemical constituents representing 99.72% of the essential oil were found, comprising menthone (39.55%), isopulegone (30.49%), eucalyptol (10.38%), and α-terpineol (3.15%);these were major components, and others were minor components. The essential oil showed strong antibacterial activity against Staphylococcus aureus [(35.24 ± 0.13) mm], Enterococcus faecalis [(32.12 ± 0.12) mm] and Bacillus cereus [(30.06 ± 0.04) mm], as well as antifungal activity against Aspergillus flavus [(38.02 ± 0.06) mm], Alternaria alternaria [(35.26 ± 0.12) mm], and Penicillum spp ((34.14 ± 0.02) mm)Conclusions: It seems that the essential oils derived from the Mentha longifolia L. species could be used as a natural source of antimicrobial agents.展开更多
文摘Minthostachys mollis(Benth)Griseb.is a variety of‘peperina’with high pulegone+methone content but with a higher menthone content.The objective was to evaluate the antioxidant activity(AA)of peperina essential oil(PEO)lower in pulegone/MENT(menthone+isomenthone)ratio combined with butylated hydroxytoluene(BHT).PEO was performed by gas chromatography with mass spectrometry(GC-MS).The thermal stability of PEO was determined at 60℃ for 28 days and analysed by GC-MS with SPME.Free Radical Scavening Activity(FRSA-DPPH test)and Total Phenol Content(TPC-analysed with Folin-cicolteau reagent)determined indirect AA.Direct AA was performed with accelerated oxidation of sunflower oil for 28 days at 60℃.Concentrations of 0.02,0.01 and 0.005%w/w(1,2 and 3)were used for the antioxidant BHT(B1,B2 and B3),PEO(P1,P2 and P3)and combinations.Chemical indicators(Conjugated Dienes,Peroxide Value and Anisidine Value),complete oxidation(Total Oxidation Value and Integral Oxidation Value)and volatiles(2-heptenal(Z)and 2,4-Decadienal(E,E))were determined.The pulegone/MENT ratio was 0.59 but the combinations B2P2 and B2P1 allow the use of lower concentration of BHT with higher efficiency.The low pulegone/MENT PEO can be combined with BHT to delay oxidation.
文摘Objective: To investigate the chemical composition, in vitro antibacterial and antifungal activity of Mentha longifolia L. essential oils using aqueous extract. Methods: Mentha longifolia L. essential oils were extracted using hydrodistillation with Clevenger apparatus for 3 h and the yield of the essential oil was calculated. Essential oils were qualitatively and quantitatively analyzed with gas chromatography and gas chromatography-mass spectrometer. For these essential oils, antibacterial and antifungal activity against human pathogens were evaluated. Results: Nineteen chemical constituents representing 99.72% of the essential oil were found, comprising menthone (39.55%), isopulegone (30.49%), eucalyptol (10.38%), and α-terpineol (3.15%);these were major components, and others were minor components. The essential oil showed strong antibacterial activity against Staphylococcus aureus [(35.24 ± 0.13) mm], Enterococcus faecalis [(32.12 ± 0.12) mm] and Bacillus cereus [(30.06 ± 0.04) mm], as well as antifungal activity against Aspergillus flavus [(38.02 ± 0.06) mm], Alternaria alternaria [(35.26 ± 0.12) mm], and Penicillum spp ((34.14 ± 0.02) mm)Conclusions: It seems that the essential oils derived from the Mentha longifolia L. species could be used as a natural source of antimicrobial agents.
