The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. Th...The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. These essential oils were able to inhibit the breakdown of phospholipids induced by venoms of snakes of the Bothrops genus. Both oils presented hemolytic activity, although the Mentha viridis (L). L. oil induced hemolysis only at the highest concentrations (14.6 and 29 μL·mL-1). The essential oil from M. viridis induced 3.9;8.6 and 16.2 times greater damage to human leukocyte DNA than that observed with the positive control (100 μg·μL-1 doxorubicin) at concentrations of 0.25;0.5 and 1.0 μL·mL-1, respectively. A similar effect was observed for the oil from M. pulegium (2.1, 2.5 and 15.8 times greater damage). The results extend the characterization of these essential oils and demonstrate their potential use in industries.展开更多
EST sequences of Mentha piperita available in the public domain(NCBI) were exploited to develop SSR markers. A total of 1316 ESTs were assembled into 155 contigs and 653 singletons and of these, 110 sequences were fou...EST sequences of Mentha piperita available in the public domain(NCBI) were exploited to develop SSR markers. A total of 1316 ESTs were assembled into 155 contigs and 653 singletons and of these, 110 sequences were found to contain 130 SSRs, with a frequency of1 SSR/3.4 kb. Dinucleotide repeat SSRs were most frequent(72.3%) with the AG/CT(43.8%)repeat motif followed by AT/AT(16.2%). Primers were successfully designed for 68SSR-containing sequences(62.0%). The 68 primers amplified 13 accessions of M. piperita and 54 produced clear amplicons of the expected size. Of these 54, 33(61%) were found to be polymorphic among M. piperita accessions, showing from 2 to 4 alleles with an average of2.33 alleles/SSR, and the polymorphic information content(PIC) value varied between 0.13 and 0.51(average 0.25). All the amplified SSRs showed transferability among four different species of Mentha, with a highest in Mentha arvensis(87.0%) and minimum in Mentha citrata(37.0%). The newly developed SSRs markers were found to be useful for diversity analysis, as they successfully differentiated among species and accessions of Mentha.展开更多
Objective:To determine the anti-bacterial efficacy of chloroform,ethanol,ethyl acetate and water extracts of inter-nodal and leaves derived calli extracts from Mentha arvensis(M.arvensis) against Salmonella typhi(S.ty...Objective:To determine the anti-bacterial efficacy of chloroform,ethanol,ethyl acetate and water extracts of inter-nodal and leaves derived calli extracts from Mentha arvensis(M.arvensis) against Salmonella typhi(S.typhi),Streptococcus pyogenes(S.pyogenes),Proteus vulgaris(P. vulgaris) and Bacillus subtilis(B.subtilis).Methods:The inter-nodal and leaves segments of M.arvensis were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3%sucrose,gelled with 0.7%agar and different concentration of 2,4-Dichlorophenoxyacetie acid(2,4-D) either alone or in combinations.The preliminary phytochemical screening was performed by Brindha et al method.Antibacterial efficacy was performed by disc diffusion method and incubated for 24 h at 37℃.Results:Maximum percentage of callus formation(inter-nodal segments 84.3±0.78;leaves segments 93.8±1.27) was obtained on Murashige and Skoog’s basal medium supplemented with 3%sucrose and 1.5 mg/L of 2,4-D.The ethanol extracts of leaves derived calli showed the maximum bio-efficacy than other solvents.The leaves and stem derived calli extracts on Proteus sp.showed that the plants can be used in the treatment of urinary tract infection associated with Proteus sp.Through the bacterial efficacy studies,it is confirmed that the in vitro raised calli tissue was more effective compared to in vivo tissue.Conclusions:The bio-efficacy study confirmed that the calli mediated tissues showed the maximum zone of inhibition.The present study paved a protocol to establish high potential cell lines by in vitro culture.展开更多
Objective: To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: Thymus satureioides(T. satureioides) and Mentha pulegium(...Objective: To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: Thymus satureioides(T. satureioides) and Mentha pulegium(M. pulegium), and to study their chemical composition.Methods: The aerial parts of T. satureioides and M. pulegium(leaves and stems) were hydro-distillated using a Clevenger-type apparatus and essential oils were analyzed and identified by gas chromatography-mass spectrometry. Antimycobacterial screening of essential oils was performed on the basis of the inhibition zone diameter by disc diffusion method against two mycobacterial strains whereas the minimal inhibitory concentration and minimal bactericidal concentration were determined by using the micro-dilution method.Results: Chemical analysis of their aerial part's essential oils gave as major compounds,borneol(34.26%), carvacrol(31.21%) and thymol(3.71%) for T. satureioides and R(+)-pulegone(75.48%), carvone(6.66%) and dihydrocarvone(4.64%) for M. pulegium.Thereafter their antimycobacterial effect evaluation, using the micro-dilution method,indicated that minimal inhibitory concentration values of T. satureioides essential oil ranged from 0.062% to 0.015%(v/v) and from 0.125% to 0.031%(v/v) for M. pulegium respectively against Mycobacterium aurum and Mycobacterium smegmatis.Conclusions: It is clearly evident from the results obtained that the Moroccan medicinal plants have great potential to be used as anti-tuberculosis agents. These findings may help scientists to undertake several research projects to discover useful natural product as new anti-tuberculosis drug.展开更多
Objective:To formulate nanoemulsion from essential oils of Mentha(M.)piperita L.and Eucalyptus(E.)globulus L.and to compare their repellant activity with normal essential oils and N,N-diethyl-m toluamide(DEET)as a sta...Objective:To formulate nanoemulsion from essential oils of Mentha(M.)piperita L.and Eucalyptus(E.)globulus L.and to compare their repellant activity with normal essential oils and N,N-diethyl-m toluamide(DEET)as a standard chemical compound.Methods:In this study,protection time of essential oils and DEET was evaluated on four human subjects using test cage,and their values were determined against Anopheles stephensi.Furthermore,ED50 values for the above essential oils were determined using the ASTM E951-94 method.The compositions of essential oils were determined using GC-MS,and droplet size and zeta potential of the nanoemulsion were measured with dynamic light scattering.Results:The results(expressed as mean±SD)showed that protection time of M.piperita 50%,M.piperita Nano 50%,E.globulus 50%,E.globulus Nano 50%,and DEET 25%was(2.89±0.45)h,(4.17±0.28)h,(0.96±0.27)h,(5.51±0.02)h,and(6.10±0.47)h,respectively.ED50 values were 29.10(95%CI:23.36-36.06)μg/cm^2 for Mentha,19.39(15.35-23.99)μg/cm^2 for Mentha Nano,36.10(28.70-48.01)μg/cm^2 for Eucalyptus,18.50(14.65-23.23)μg/cm^2 for Eucalyptus Nano,and 3.62(2.68-4.55)μg/cm^2 for DEET,respectively.E.globulus Nano and M.piperita Nano provided significantly longer protection than normal essential oils E.globulus and M.piperita(P<0.01).Conclusions:The preparation of nanoemulsion from the essential oils of M.piperita and E.globulus,significantly increases the protection time and reduces ED50 values of these essential oils,hence,M.piperita Nano and E.globulus Nano can be good alternatives to DEET and other chemical compounds.展开更多
Objective:To investigate the total phenolics,flavonoids and tannins content and the in vitro antioxidant activities of methanolic extracts of six wild Mentha species which are Mentha aquatica,Mentha arvensis,Mentha pi...Objective:To investigate the total phenolics,flavonoids and tannins content and the in vitro antioxidant activities of methanolic extracts of six wild Mentha species which are Mentha aquatica,Mentha arvensis,Mentha piperita,Mentha pulegium,Mentha rotundifolia and Mentha villosa.Methods:The Folin–Ciocalteu method was used to determine the total phenols content while flavonoids were estimated according to the aluminum chloride colorimetric method.To evaluate tannins content,vanillin and HCl were added to methanolic extracts.The antioxidant potential was measured by 1,1-diphenyl-2-picrylhydrazyl radical scavenging,ferrous ion chelating and the inhibition of b-carotene bleaching assays.Results:The methanol extracts of Algerian mints were rich in phenolic compounds and exhibited powerful antioxidant activity ranging from 7.5 mg/mL to 44.66 mg/mL,which varied significantly among species.Mentha aquatica stood out with efficient antioxidant ability which was correlated to the high total phenolics content,followed by Mentha arvensis and Mentha piperita with very close values,comparing to Mentha pulegium,Mentha rotundifolia and Mentha villosa with lowest values.Conclusions:These results show that methanolic extracts of Mentha species from Algeria have a great potential of polyphenols which can be used as a natural food preservative and antioxidant source.展开更多
Objective:To assess the larvicidal and repellent potential of the essential oil extracted from the leaves of peppermint plant,Mentha piperita(M.piperita) against the larval and adult stages of Aedes aegypti(Ae.Aegypti...Objective:To assess the larvicidal and repellent potential of the essential oil extracted from the leaves of peppermint plant,Mentha piperita(M.piperita) against the larval and adult stages of Aedes aegypti(Ae.Aegypti).Methods:The larvicidal potential of peppermint oil was evaluated against early fourth instar larvae of Ae.aegypti using WHO protocol.The mortality counts were made after 24 and 48 h,and LC_(50) and LC_(90) values were calculated.The efficacy of peppermint oil as mosquito repellent was assessed using the human-bait technique.The measured area of one arm of a human volunteer was applied with the oil and the other arm was applied with ethanol.The mosquito bites on both the arms were recorded for 3 min after every 15 min.The experiment continued for 3 h and the percent protection was calculated.Results:The essential oil extracted from M.piperita possessed excellent larvicidal efficiency against dengue vector. The bioassays showed an LC_(50) and LC_(90) value of 111.9 and 295.18 ppm,respectively after 24 h of exposure.The toxicity of the oil increased 11.8%when the larvae were exposed to the oil for 48 h.The remarkable repellent properties of M.piperita essential oil were established against adults Ae.aegypti.The application of oil resulted in 100%protection till 150 min.After next 30 min, only 1-2 bites were recorded as compared with 8-9 bites on the control arm.Conclusions:The peppermint essential oil is proved to be efficient larvicide and repellent against dengue vector. Further studies are needed to identify the possible role of oil as adulticide,oviposilion deterrent and ovicidal agent.The isolation of active ingredient from the oil could help in formulating strategies for mosquito control.展开更多
Objective:To investigate potential antioxidant,antimicrobial,cytotoxic and analgesic activities of ethanolic extract of Mentha arrensis l.,in different in vivo and in vitro experimental models.Methods:In vitro DPPH ra...Objective:To investigate potential antioxidant,antimicrobial,cytotoxic and analgesic activities of ethanolic extract of Mentha arrensis l.,in different in vivo and in vitro experimental models.Methods:In vitro DPPH radical scavenging assay was used to evaluate the antioxidant activity of the plant extract.In vivo analgesic activity was carried out by acetic acid—induced writhing test in Swiss albino mice.All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight.Antibacterial activilv was studied by disk diffusion assay against some Gram—positive and Gram—negative bacterial strains.Brine shrimp lethality assay was used to investigate cyloloxicity effects of the plant extract.Results:The extract showed free radical scavenging activity in the DPPH assay(IC<sub>50</sub><sup>4</sup>1 μg/mL)compared to the standard antioxidant ascorbic acid(IC<sub>50</sub><sup>1</sup>9 μg/mL).The extract also produced prominent antimicrobial activity against Salmonella typhi.Salmonella paratyphi.Shigella boydii,Streptococcus pyogenes and Streptococcus aureus compared to standard drug kanamycin at the dose of 30 μg/disc.The extract exhibited lethality against the brine shrimp nauplii with the LC<sub>50</sub>,values of 40 μg/mL.and also 90%mortality(LC<sub>90</sub>) value was found to be 160 μg/mL.In analgesic test.the extract demonstrated statistically significant（P【0.01） analgesic effect in acetic acid induced writhing in white albino mice al both dose levels.Conclusions:These results suggest that the ethanolic extract of Mentha arvenns L.has potential antioxidant,antibacterial,cytotoxic and analgesic activities that support the ethnopharmacological uses of this plant.展开更多
Mentha haplocalyx(Mentha canadensis)is widely used as a medicinal plant in traditional Chinese medicine,and the extracts of its aerial parts are found to signifcantly inhibit the activity ofα-glucosidase with an IC_(...Mentha haplocalyx(Mentha canadensis)is widely used as a medicinal plant in traditional Chinese medicine,and the extracts of its aerial parts are found to signifcantly inhibit the activity ofα-glucosidase with an IC_(50) value of 21.0μg/mL.Bioactivity-guided isolation of the extracts aforded two new compounds(1 and 2),together with 23 known ones(3-25).Their structures were established by extensive spectroscopic analyses(1D and 2D NMR,MS,IR and UV).Compounds 1-17 and 21-25 were evaluated for theirα-glucosidase inhibitory activities.Compound 11 was the most active ones with an IC_(50) values of 83.4μM.These results verify theα-glucosidase inhibitory activity of M.haplocalyx(M.canadensis)and specify its active compounds for the frst time.展开更多
Mentha haplocalyx(mint)is a significant traditional Chinese medicine(TCM)listed in the Catalogue of’Medicinal and Food Homology’,therefore,its geographical origins(GOs)are critical to the medicinal and food value.La...Mentha haplocalyx(mint)is a significant traditional Chinese medicine(TCM)listed in the Catalogue of’Medicinal and Food Homology’,therefore,its geographical origins(GOs)are critical to the medicinal and food value.Laser-induced breakdown spectroscopy(LIBS)is an advanced analytical technique for GOs certification,due to the fast multi-elemental analysis requiring minimal sample pretreatment.In this study,LIBS data of sampled mint from five GOs were investigated by LIBS coupled with multivariate statistical analyzes.The spectral data was analyzed by two chemometric algorithms,i.e.principal component analysis(PCA)and least squares support vector machines(LS-SVM).Specifically,the performance of LS-SVM with least kernel and radial basis function(RBF)kernel was explored in sensitivity and robustness tests.Both LS-SVM algorithms exhibited excellent performance of classification in sensitive test and good performance(a little inferior)in robustness test.Generally,LS-SVM with linear kernel equally outperformed LS-SVM based on RBF kernel.The result indicated the potential for future applications in herbs and food,especially for in situ GOs applications of TCM authenticity rapidly.展开更多
This study was designed to evaluate antimicrobial and antioxidant activities of the essential oil and methanol extract from Mentha longifolia L. hudson. Antioxidant activity was evaluated through 2,2-diphenyl-l-picryl...This study was designed to evaluate antimicrobial and antioxidant activities of the essential oil and methanol extract from Mentha longifolia L. hudson. Antioxidant activity was evaluated through 2,2-diphenyl-l-picrylhydrazyl （DPPH） assay and β-carotene/linoleic acid assay. The essential oil and methanol extract were individually tested [disc-diffusion assay and evaluating minimum inhibition concentration （MIC）] against a number of bacteria. The essential oil showed strong antimicrobial activity against bacteria tested whereas the methanol extract almost remained inactive. In contrast, the extract showed much better activity than the essential oil in antioxidant activity assays employed, e.g. in the inhibition of free radical DPPH and β-carotene/linolcic acid systems. In the former, the extract was able to reduce the stable free radical DPPH with an IC50 of 55.3μg/mL while that of the oils were 10,630μg/mL. When compared to BHT （butylated hydroxytoluene）, a synthetic antioxidant, both showed weaker antioxidative potential. Similarly, in β-carotene/linoleic acid assay, these samples were not effectively able to inhibit the linoleic acid oxidation; exhibiting only 24% and 36% inhibitions at 2 mg/mL, respectively; both were far below than that of BHT. Total phenolic constituent of the extract was approximately 4.5 g/100 g as gallic acid equivalent. Gas chromatography-mass spectroscopy （GC-MS） analysis of the oil resulted in the identification of 45 constituents, ei^-piperitone epoxide, pulegone and piperitenone oxide being the main components. The results indicate that essential oil and extract ofM. longifolia L. hudson may be used as natural preservative in food against the agents of foodborne diseases and food spoilage.展开更多
The chemical composition of essential oils obtained from Artemisia herba-alba and Mentha pulegium were determined. The essential oils were analyzed by gas chromatography coupled with mass spectrometry （GC/MS）. Their...The chemical composition of essential oils obtained from Artemisia herba-alba and Mentha pulegium were determined. The essential oils were analyzed by gas chromatography coupled with mass spectrometry （GC/MS）. Their antibacterial activity was studied in vitro against three standard strains： E. coli ATCC 25922, Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, and five clinical strains： Enterobacter cloacae, Staphylococcus aureus, Pseudomonas pyocyanique, Enterococcus faecium, and E. coli. Nineteen constituents were identified in A. herba-alba essential oil representing 99.57% of the total composition The major component was α-thujone （59.07%）. The bacterial strains were inhibited at concentrations ranging from 1.25 μL/mL to 5μL/mL and killed at concentrations ranging from 1.25 μL/mL to 10 μL/mL. M. pulegium resulted in the identification of eighteen constituents representing 99.48% of the total composition. The main component was pulegone （78.07%）. The minimal inhibitory （MIC） and bactericidal （MBC） concentrations were ranging from 1.25 μL/mL to 2.5 μL/mL.展开更多
Objective: To determin the chemical compounds of Mentha suaveolens(M. suaveolens) and Pinus halepensis(P. halepensis) essential oils(Eos) and evaluate their antioxidant and antibacterial activities. Methods: The chemi...Objective: To determin the chemical compounds of Mentha suaveolens(M. suaveolens) and Pinus halepensis(P. halepensis) essential oils(Eos) and evaluate their antioxidant and antibacterial activities. Methods: The chemical composition of P. halepensis and M. suaveolens EOs was determined by GC-MS analysis. The antioxidant activity was evaluated using DPPH,ABTS and FRAP assays. The antibacterial effect was tested against 6 bacterial strains using the well diffusion method and micro-dilution assay. Results: The major components of P. halepensis EOs were β-caryophyllene(28.04%),myrcene(23.81%) and α-pinene(12.02%). However,piperitenone oxid(56.28%),piperitenone(11.64%) and pulegone(6.16%) were the major components of M. suaveolens EOs. M. suaveolens EOs showed remarkable antioxidant activities compared with P. halepensis EOs,showing antioxidant capacity values of IC50=(64.76±2.24) μg/mL,IC50=(82.73±3.34) μg/mL,and IC50=(93.35±4.45) μg/mL,revealed by DPPH,ABTS and FRAP assays,respectively. However,P. halepensis EOs showed interesting antibacterial effects against all bacterial strains. The most sensible strains to P. halepensis EOs were Staphylococcus aureus [(34.00±0.50) mm],Listeria monocytogenes [(31.00±1.50)] mm and Proteus mirabilis [(29.00±2.25)mm]. Furthermore,the lowest minimal inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) values were revealed by P. halepensis EOs against Staphylococcus aureus [MIC=MBC=0.125%(v/v)] and Listeria monocytogenes [MIC=MBC=0.25%(v/v)]. Conclusions: P. halepensis and M. suaveolens EOs contain bioactive compounds that could have potential applications against bacterial infections and oxidative stress related diseases as well as for food conservation. However,further investigations are necessary to isolate and investigate the action mechanisms of these bioactive compounds.展开更多
AIM:To examine the antiulcerogenic effects of various extracts of Mentha arvensis Linn on acid,ethanol and pylorus ligated ulcer models in rats and mice.METHODS:Various crude extracts of petroleum ether,chloroform,or ...AIM:To examine the antiulcerogenic effects of various extracts of Mentha arvensis Linn on acid,ethanol and pylorus ligated ulcer models in rats and mice.METHODS:Various crude extracts of petroleum ether,chloroform,or aqueous at a dose of 2 g/kg po did not produce any signs or symptoms of toxicity in treated animals.In the pyloric ligation model oral administration of different extracts such as petroleum ether,chloroform and aqueous at 375 mg/kg po,standard drug ranitidine 60 mg/kg po and control group 1 Tween 80,5 mL/kg po to separate groups of Wister rats of either sex(n = 6) was performed.Total acidity,ulcer number,scoring,incidence,area,and ulcer index were assessed.RESULTS:There was a decrease in gastric secretion and ulcer index among the treated groups i.e.petroleum ether(53.4),chloroform(59.2),aqueous(67.0) and in standard drug(68.7) when compared to the negative control.In the 0.6 mol/L HCl induced ulcer model in rats(n = 6) there was a reduction in ulcerative score in animals receiving petroleum ether(50.5),chloroform(57.4),aqueous(67.5) and standard.drug(71.2) when compared to the negative control.In the case of the 90 ethanol-induced ulceration model(n = 6) in mice,there was a decrease in ulcer score in test groups of petroleum ether(53.11),chloroform(62.9),aqueous(65.4) and standard drug ranitidine(69.7) when compared to the negative control.It was found that pre-treatment with various extracts of Mentha arvensis Linn in three rat/mice ulcer models ie ibuprofen plus pyloric ligation,0.6 mol/L HCl and 90 ethanol produced significant action against acid secretion(49.3 ± 0.49 vs 12.0 ± 0.57,P < 0.001).Pre-treatment with various extracts of Mentha arvensis Linn showed highly-significant activity against gastric ulcers(37.1 ± 0.87 vs 12.0 ± 0.57,P < 0.001).CONCLUSION:Various extracts of Mentha arvensis Linn.375 mg/kg body weight clearly shows a protective effect against acid secretion and gastric ulcers in ibuprofen plus pyloric ligation,0.6 mol/L HCl induced and 90 ethanol-induced ulcer models.展开更多
[Objective] The research aimed to study the antibacterial action of Mentha spicata Linn extract in vitro.[Method] The cylinder-plate method was used to measure the inhibitory zone size of Mentha spicata Linn extract.T...[Objective] The research aimed to study the antibacterial action of Mentha spicata Linn extract in vitro.[Method] The cylinder-plate method was used to measure the inhibitory zone size of Mentha spicata Linn extract.The test-tube double dilution method was used to measure the minimal inhibitory concentration(MIC)of Mentha spicata Linn extract on the four kinds of animal pathogenic bacteria,which could determine the antibacterial effect.[Result] The decoction,alcohol extract and volatile oil of Mentha spicata Linn had the different antibacterial effects on the four kinds of animal pathogenic bacteria.Especially the inhibition effect on the pig staphylococcus was comparatively obvious.MIC of Mentha spicata Linn decoction I,alcohol extract III,volatile oil V and volatile oil VI were respectively 31.25,62.5,2.32 and 2.31 mg/ml.[Conclusion] The decoction,alcohol extract and volatile oil of Mentha spicata Linn had the certain antibacterial effect in vitro,and the extracts which were gained by the different extraction methods had the different inhibition effects on the experimental bacteria.展开更多
The effects of plant growth regulators on peppermint (Mentha piperita) cultured in vitro were studied for the purpose of maximizing growth and essential oil production in micropropagated plants. The basal medium was e...The effects of plant growth regulators on peppermint (Mentha piperita) cultured in vitro were studied for the purpose of maximizing growth and essential oil production in micropropagated plants. The basal medium was experimentally supplemented with the auxin 4-indol-3-ylbutyric acid (IBA) and the cytokinin 6-benzylaminopurine (BAP) individually and in combination. Supplementation with BAP alone resulted in the highest values for root length, root dry weight, shoot length, and numbers of nodes, leaves, and ramifications. Treatment with IBA alone or with IBA + BAP resulted in a ~50% increase in shoot fresh weight. The production of secondary metabolites was affected only by the addition of cytokinin, which resulted in a ~40% increase in the total yield of essential oils (EOs). Similar trends were observed for yields of the major EO components (menthone, menthol, pulegone, and menthofuran). Our findings demonstrate that the application of growth regulators increases EO production and biomass concomitantly in an herbaceous species rich in commercially valuable terpenes.展开更多
Mentha piperita L. (Peppermint) is a perennial glabrous and strongly scented herb belonging to family Lamiaceae. The plant is aromatic, stimulant and used for allaying nausea, headache and vomiting. Its oil is one of ...Mentha piperita L. (Peppermint) is a perennial glabrous and strongly scented herb belonging to family Lamiaceae. The plant is aromatic, stimulant and used for allaying nausea, headache and vomiting. Its oil is one of the most popular widely used essential oils in food products, cosmetics, pharmaceuticals, dental preparations, mouthwashes, soaps and alcoholic liquors. The antibacterial potential of six extracts from leaf, stem and root of Mentha piperita against pathogenic bacteria such as Bacillus subtilis, Streptococcus pneumonia, Staphylococcus aureus, Escherichia coli, Proteus vulgaris and Klebsiella pneumonia were evaluated by agar well diffusion method. The organic (ethanol, methanol, ethyl acetate, chloroform, hexane and petroleum ether) extracts of the leaves were found to possess strong antibacterial activity against a range of pathogenic bacteria. The ethyl acetate leaf extract of Mentha piperita showed pronounced inhibition than chloroform, petroleum ether and hexane. The leaf extract activity being more on Bacillus subtilis, Staphylococcus aureus and Proteus vulgaris than Escherichia coli, Streptococcus pneumonia and Klebsiella pneumonia. In the present study, we also evaluated the phytochemical analysis for the presence of various secondary metabolites. The analysis revealed the presence of alkaloids, flavonoids, steroids, tannins, and phenols.展开更多
Purpose: Premature ovarian failure (POF) includes cessation of normal ovarian function before age 40, causing amenorrhea, menopausal symptoms and general health problems. Hormone replacement therapy (HRT) can be admin...Purpose: Premature ovarian failure (POF) includes cessation of normal ovarian function before age 40, causing amenorrhea, menopausal symptoms and general health problems. Hormone replacement therapy (HRT) can be administered in order to prevent menopausal symptoms, osteoporosis and vascular complications. Due to the contraindications and side effects of HRT and the increasing demand for alternative therapeutic modalities, we used Mentha longifolia, which is known in the Iranian traditional medicine (ITM), as being able to cause menstrual bleeding in women with early amenorrhea and decreased FSH serum. Methods: In this pilot study, we report twenty seven amenorrheic women with POF who were referred to the Iranian traditional medicine clinic in Tehran and treated by Mentha longifolia. All those patients had high FSH levels and amenorrhea. They were in their fertility age, between 30 to 40 years old. They were asked to take a cup of herbal tea which was steeped with Mentha longifolia tea three times a day for 2 weeks in 3 menstural cycles. If the patients developed menstruation, the FSH serum level was measured immediately;otherwise they had an FSH measurement one month after the beginning of therapy. Results: Mean age of patients was 36.44 ± 1.5 years. Mean FSH level before treatment was 79.13 ± 19.17 mIU/ml, and after treatment was 27.83 ± 16.14 mIU/ml. In four patients who did not have any response, mean range of FSH was 82.37 ± 19.75 mIU/ml before treatment and afterwards it was 81.97 ± 21.52 mIU/ml. After treatment with herbal teas, there was a significant decrease in FSH (79.39 ± 19.17 to 27.83 ± 16.14 mIU/ml, p value < 0.001). All patient except four (14.81%) had mensturation after taking the herbal medicine. Those four patients showed no decrease in FSH level. More of the patients who used medicinal tea achieved regular monthly bleeding when followed for three cycles.展开更多
Rumex abyssinica Jacq. (Polygonaceae) is locally used in management of allergies and female reproductive healthcare;whereas Mentha spicata L. (Lamiaceae) is used to treat gastrointestinal and respiratory distress, dan...Rumex abyssinica Jacq. (Polygonaceae) is locally used in management of allergies and female reproductive healthcare;whereas Mentha spicata L. (Lamiaceae) is used to treat gastrointestinal and respiratory distress, dandruff, halitosis and malaria in Uganda. Owing to the paucity of data on their safety profiles, this study evaluated the acute and sub-acute toxicities of 70% ethanolic leaf extracts of both plants in mice and Wistar albino rats. The oral acute toxicity of both plants was evaluated in Swiss mice of 7-8 weeks old (16-22 g) body weight and LD50 determined. Sub-acute toxicity was evaluated in Wistar albino rats (6 per group) at dose rates of 500, 1000 and 1500 mg/kg for 28 days. The LD50 of R. abyssinica and M. spicata in mice was 7727 mg/kg and 13,606 mg/kg body weight, respectively. General signs of toxicity due to large doses of both plants extract included hyperurination, abdominal muscle twitches and convulsions. In the sub-acute toxicity test, rats treated with both extracts did not exhibit any clinical signs of toxicity;no mortality and changes in body weight were observed. R. abyssinica did not cause significant changes in haematological indices, except a significant increase in HCT (p 0.05). However, a dose dependant significant decrease in HCT (p 0.05) and a significant increase (p 0.05) in the levels of WBC, LMY and MCHC were observed in rats treated with M. spicata extract. Biochemical test showed that both extracts caused a significant dose dependent increase (p 0.05) in levels of ALT and ALP. Marked increase in the levels of AST was also observed in rats treated with M. spicata extract. Of the two extracts, only rats treated with </展开更多
基金the support of the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico(CNPq) the Fundacao de Amparo a Pesquisa do Estado de Minas Gerais(FAPEMIG)for financial support the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior(CAPES)for a PVNS.
文摘The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. These essential oils were able to inhibit the breakdown of phospholipids induced by venoms of snakes of the Bothrops genus. Both oils presented hemolytic activity, although the Mentha viridis (L). L. oil induced hemolysis only at the highest concentrations (14.6 and 29 μL·mL-1). The essential oil from M. viridis induced 3.9;8.6 and 16.2 times greater damage to human leukocyte DNA than that observed with the positive control (100 μg·μL-1 doxorubicin) at concentrations of 0.25;0.5 and 1.0 μL·mL-1, respectively. A similar effect was observed for the oil from M. pulegium (2.1, 2.5 and 15.8 times greater damage). The results extend the characterization of these essential oils and demonstrate their potential use in industries.
文摘EST sequences of Mentha piperita available in the public domain(NCBI) were exploited to develop SSR markers. A total of 1316 ESTs were assembled into 155 contigs and 653 singletons and of these, 110 sequences were found to contain 130 SSRs, with a frequency of1 SSR/3.4 kb. Dinucleotide repeat SSRs were most frequent(72.3%) with the AG/CT(43.8%)repeat motif followed by AT/AT(16.2%). Primers were successfully designed for 68SSR-containing sequences(62.0%). The 68 primers amplified 13 accessions of M. piperita and 54 produced clear amplicons of the expected size. Of these 54, 33(61%) were found to be polymorphic among M. piperita accessions, showing from 2 to 4 alleles with an average of2.33 alleles/SSR, and the polymorphic information content(PIC) value varied between 0.13 and 0.51(average 0.25). All the amplified SSRs showed transferability among four different species of Mentha, with a highest in Mentha arvensis(87.0%) and minimum in Mentha citrata(37.0%). The newly developed SSRs markers were found to be useful for diversity analysis, as they successfully differentiated among species and accessions of Mentha.
文摘Objective:To determine the anti-bacterial efficacy of chloroform,ethanol,ethyl acetate and water extracts of inter-nodal and leaves derived calli extracts from Mentha arvensis(M.arvensis) against Salmonella typhi(S.typhi),Streptococcus pyogenes(S.pyogenes),Proteus vulgaris(P. vulgaris) and Bacillus subtilis(B.subtilis).Methods:The inter-nodal and leaves segments of M.arvensis were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3%sucrose,gelled with 0.7%agar and different concentration of 2,4-Dichlorophenoxyacetie acid(2,4-D) either alone or in combinations.The preliminary phytochemical screening was performed by Brindha et al method.Antibacterial efficacy was performed by disc diffusion method and incubated for 24 h at 37℃.Results:Maximum percentage of callus formation(inter-nodal segments 84.3±0.78;leaves segments 93.8±1.27) was obtained on Murashige and Skoog’s basal medium supplemented with 3%sucrose and 1.5 mg/L of 2,4-D.The ethanol extracts of leaves derived calli showed the maximum bio-efficacy than other solvents.The leaves and stem derived calli extracts on Proteus sp.showed that the plants can be used in the treatment of urinary tract infection associated with Proteus sp.Through the bacterial efficacy studies,it is confirmed that the in vitro raised calli tissue was more effective compared to in vivo tissue.Conclusions:The bio-efficacy study confirmed that the calli mediated tissues showed the maximum zone of inhibition.The present study paved a protocol to establish high potential cell lines by in vitro culture.
基金Supported by the Cooperation for Innovation in the Agro-Food Domain Project within the 7th Research and Development Framework Programme FP7-INCO-2013-9(Grant agreement number 609495)
文摘Objective: To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: Thymus satureioides(T. satureioides) and Mentha pulegium(M. pulegium), and to study their chemical composition.Methods: The aerial parts of T. satureioides and M. pulegium(leaves and stems) were hydro-distillated using a Clevenger-type apparatus and essential oils were analyzed and identified by gas chromatography-mass spectrometry. Antimycobacterial screening of essential oils was performed on the basis of the inhibition zone diameter by disc diffusion method against two mycobacterial strains whereas the minimal inhibitory concentration and minimal bactericidal concentration were determined by using the micro-dilution method.Results: Chemical analysis of their aerial part's essential oils gave as major compounds,borneol(34.26%), carvacrol(31.21%) and thymol(3.71%) for T. satureioides and R(+)-pulegone(75.48%), carvone(6.66%) and dihydrocarvone(4.64%) for M. pulegium.Thereafter their antimycobacterial effect evaluation, using the micro-dilution method,indicated that minimal inhibitory concentration values of T. satureioides essential oil ranged from 0.062% to 0.015%(v/v) and from 0.125% to 0.031%(v/v) for M. pulegium respectively against Mycobacterium aurum and Mycobacterium smegmatis.Conclusions: It is clearly evident from the results obtained that the Moroccan medicinal plants have great potential to be used as anti-tuberculosis agents. These findings may help scientists to undertake several research projects to discover useful natural product as new anti-tuberculosis drug.
文摘Objective:To formulate nanoemulsion from essential oils of Mentha(M.)piperita L.and Eucalyptus(E.)globulus L.and to compare their repellant activity with normal essential oils and N,N-diethyl-m toluamide(DEET)as a standard chemical compound.Methods:In this study,protection time of essential oils and DEET was evaluated on four human subjects using test cage,and their values were determined against Anopheles stephensi.Furthermore,ED50 values for the above essential oils were determined using the ASTM E951-94 method.The compositions of essential oils were determined using GC-MS,and droplet size and zeta potential of the nanoemulsion were measured with dynamic light scattering.Results:The results(expressed as mean±SD)showed that protection time of M.piperita 50%,M.piperita Nano 50%,E.globulus 50%,E.globulus Nano 50%,and DEET 25%was(2.89±0.45)h,(4.17±0.28)h,(0.96±0.27)h,(5.51±0.02)h,and(6.10±0.47)h,respectively.ED50 values were 29.10(95%CI:23.36-36.06)μg/cm^2 for Mentha,19.39(15.35-23.99)μg/cm^2 for Mentha Nano,36.10(28.70-48.01)μg/cm^2 for Eucalyptus,18.50(14.65-23.23)μg/cm^2 for Eucalyptus Nano,and 3.62(2.68-4.55)μg/cm^2 for DEET,respectively.E.globulus Nano and M.piperita Nano provided significantly longer protection than normal essential oils E.globulus and M.piperita(P<0.01).Conclusions:The preparation of nanoemulsion from the essential oils of M.piperita and E.globulus,significantly increases the protection time and reduces ED50 values of these essential oils,hence,M.piperita Nano and E.globulus Nano can be good alternatives to DEET and other chemical compounds.
基金Supported by Ministry of Higher Education Scientific Research and Technology,Algeria(Grant No.F03120130013/2014)
文摘Objective:To investigate the total phenolics,flavonoids and tannins content and the in vitro antioxidant activities of methanolic extracts of six wild Mentha species which are Mentha aquatica,Mentha arvensis,Mentha piperita,Mentha pulegium,Mentha rotundifolia and Mentha villosa.Methods:The Folin–Ciocalteu method was used to determine the total phenols content while flavonoids were estimated according to the aluminum chloride colorimetric method.To evaluate tannins content,vanillin and HCl were added to methanolic extracts.The antioxidant potential was measured by 1,1-diphenyl-2-picrylhydrazyl radical scavenging,ferrous ion chelating and the inhibition of b-carotene bleaching assays.Results:The methanol extracts of Algerian mints were rich in phenolic compounds and exhibited powerful antioxidant activity ranging from 7.5 mg/mL to 44.66 mg/mL,which varied significantly among species.Mentha aquatica stood out with efficient antioxidant ability which was correlated to the high total phenolics content,followed by Mentha arvensis and Mentha piperita with very close values,comparing to Mentha pulegium,Mentha rotundifolia and Mentha villosa with lowest values.Conclusions:These results show that methanolic extracts of Mentha species from Algeria have a great potential of polyphenols which can be used as a natural food preservative and antioxidant source.
文摘Objective:To assess the larvicidal and repellent potential of the essential oil extracted from the leaves of peppermint plant,Mentha piperita(M.piperita) against the larval and adult stages of Aedes aegypti(Ae.Aegypti).Methods:The larvicidal potential of peppermint oil was evaluated against early fourth instar larvae of Ae.aegypti using WHO protocol.The mortality counts were made after 24 and 48 h,and LC_(50) and LC_(90) values were calculated.The efficacy of peppermint oil as mosquito repellent was assessed using the human-bait technique.The measured area of one arm of a human volunteer was applied with the oil and the other arm was applied with ethanol.The mosquito bites on both the arms were recorded for 3 min after every 15 min.The experiment continued for 3 h and the percent protection was calculated.Results:The essential oil extracted from M.piperita possessed excellent larvicidal efficiency against dengue vector. The bioassays showed an LC_(50) and LC_(90) value of 111.9 and 295.18 ppm,respectively after 24 h of exposure.The toxicity of the oil increased 11.8%when the larvae were exposed to the oil for 48 h.The remarkable repellent properties of M.piperita essential oil were established against adults Ae.aegypti.The application of oil resulted in 100%protection till 150 min.After next 30 min, only 1-2 bites were recorded as compared with 8-9 bites on the control arm.Conclusions:The peppermint essential oil is proved to be efficient larvicide and repellent against dengue vector. Further studies are needed to identify the possible role of oil as adulticide,oviposilion deterrent and ovicidal agent.The isolation of active ingredient from the oil could help in formulating strategies for mosquito control.
基金Supported by Pharmacy Discipline.Life Science School,Khulna University(Grant No.Kt:PHRM:2012/April-081134)
文摘Objective:To investigate potential antioxidant,antimicrobial,cytotoxic and analgesic activities of ethanolic extract of Mentha arrensis l.,in different in vivo and in vitro experimental models.Methods:In vitro DPPH radical scavenging assay was used to evaluate the antioxidant activity of the plant extract.In vivo analgesic activity was carried out by acetic acid—induced writhing test in Swiss albino mice.All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight.Antibacterial activilv was studied by disk diffusion assay against some Gram—positive and Gram—negative bacterial strains.Brine shrimp lethality assay was used to investigate cyloloxicity effects of the plant extract.Results:The extract showed free radical scavenging activity in the DPPH assay(IC<sub>50</sub><sup>4</sup>1 μg/mL)compared to the standard antioxidant ascorbic acid(IC<sub>50</sub><sup>1</sup>9 μg/mL).The extract also produced prominent antimicrobial activity against Salmonella typhi.Salmonella paratyphi.Shigella boydii,Streptococcus pyogenes and Streptococcus aureus compared to standard drug kanamycin at the dose of 30 μg/disc.The extract exhibited lethality against the brine shrimp nauplii with the LC<sub>50</sub>,values of 40 μg/mL.and also 90%mortality(LC<sub>90</sub>) value was found to be 160 μg/mL.In analgesic test.the extract demonstrated statistically significant（P【0.01） analgesic effect in acetic acid induced writhing in white albino mice al both dose levels.Conclusions:These results suggest that the ethanolic extract of Mentha arvenns L.has potential antioxidant,antibacterial,cytotoxic and analgesic activities that support the ethnopharmacological uses of this plant.
基金supported by the CAS Hundred Talents Program,the Youth Innovation Promotion Association of the Chinese Academy of Sciences(CN),and Southeast Asia Biodiversity Research Institute,CAS(2017CASSEABRIQG003).
文摘Mentha haplocalyx(Mentha canadensis)is widely used as a medicinal plant in traditional Chinese medicine,and the extracts of its aerial parts are found to signifcantly inhibit the activity ofα-glucosidase with an IC_(50) value of 21.0μg/mL.Bioactivity-guided isolation of the extracts aforded two new compounds(1 and 2),together with 23 known ones(3-25).Their structures were established by extensive spectroscopic analyses(1D and 2D NMR,MS,IR and UV).Compounds 1-17 and 21-25 were evaluated for theirα-glucosidase inhibitory activities.Compound 11 was the most active ones with an IC_(50) values of 83.4μM.These results verify theα-glucosidase inhibitory activity of M.haplocalyx(M.canadensis)and specify its active compounds for the frst time.
基金supported by National Natural Science Foundation of China(Nos.81903796,81603396 and 31870338)the National Key Research and Development Program of China(No.2019YFC1711200)+2 种基金Major new drug innovation project of the ministry of science and technology(2018ZX09201011)Scientific and Technological Planning Projects of Colleges and Universities of Shandong Province(No.J18KA287)Binzhou Medical University Research Startup Fund Project(No.BY2016KYQD02)。
文摘Mentha haplocalyx(mint)is a significant traditional Chinese medicine(TCM)listed in the Catalogue of’Medicinal and Food Homology’,therefore,its geographical origins(GOs)are critical to the medicinal and food value.Laser-induced breakdown spectroscopy(LIBS)is an advanced analytical technique for GOs certification,due to the fast multi-elemental analysis requiring minimal sample pretreatment.In this study,LIBS data of sampled mint from five GOs were investigated by LIBS coupled with multivariate statistical analyzes.The spectral data was analyzed by two chemometric algorithms,i.e.principal component analysis(PCA)and least squares support vector machines(LS-SVM).Specifically,the performance of LS-SVM with least kernel and radial basis function(RBF)kernel was explored in sensitivity and robustness tests.Both LS-SVM algorithms exhibited excellent performance of classification in sensitive test and good performance(a little inferior)in robustness test.Generally,LS-SVM with linear kernel equally outperformed LS-SVM based on RBF kernel.The result indicated the potential for future applications in herbs and food,especially for in situ GOs applications of TCM authenticity rapidly.
文摘This study was designed to evaluate antimicrobial and antioxidant activities of the essential oil and methanol extract from Mentha longifolia L. hudson. Antioxidant activity was evaluated through 2,2-diphenyl-l-picrylhydrazyl （DPPH） assay and β-carotene/linoleic acid assay. The essential oil and methanol extract were individually tested [disc-diffusion assay and evaluating minimum inhibition concentration （MIC）] against a number of bacteria. The essential oil showed strong antimicrobial activity against bacteria tested whereas the methanol extract almost remained inactive. In contrast, the extract showed much better activity than the essential oil in antioxidant activity assays employed, e.g. in the inhibition of free radical DPPH and β-carotene/linolcic acid systems. In the former, the extract was able to reduce the stable free radical DPPH with an IC50 of 55.3μg/mL while that of the oils were 10,630μg/mL. When compared to BHT （butylated hydroxytoluene）, a synthetic antioxidant, both showed weaker antioxidative potential. Similarly, in β-carotene/linoleic acid assay, these samples were not effectively able to inhibit the linoleic acid oxidation; exhibiting only 24% and 36% inhibitions at 2 mg/mL, respectively; both were far below than that of BHT. Total phenolic constituent of the extract was approximately 4.5 g/100 g as gallic acid equivalent. Gas chromatography-mass spectroscopy （GC-MS） analysis of the oil resulted in the identification of 45 constituents, ei^-piperitone epoxide, pulegone and piperitenone oxide being the main components. The results indicate that essential oil and extract ofM. longifolia L. hudson may be used as natural preservative in food against the agents of foodborne diseases and food spoilage.
文摘The chemical composition of essential oils obtained from Artemisia herba-alba and Mentha pulegium were determined. The essential oils were analyzed by gas chromatography coupled with mass spectrometry （GC/MS）. Their antibacterial activity was studied in vitro against three standard strains： E. coli ATCC 25922, Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, and five clinical strains： Enterobacter cloacae, Staphylococcus aureus, Pseudomonas pyocyanique, Enterococcus faecium, and E. coli. Nineteen constituents were identified in A. herba-alba essential oil representing 99.57% of the total composition The major component was α-thujone （59.07%）. The bacterial strains were inhibited at concentrations ranging from 1.25 μL/mL to 5μL/mL and killed at concentrations ranging from 1.25 μL/mL to 10 μL/mL. M. pulegium resulted in the identification of eighteen constituents representing 99.48% of the total composition. The main component was pulegone （78.07%）. The minimal inhibitory （MIC） and bactericidal （MBC） concentrations were ranging from 1.25 μL/mL to 2.5 μL/mL.
基金supported by le Centre National pour la Recherche Scientifique et Technique et l’Agence Nationale des Plantes Médicinales et Aromatiques
文摘Objective: To determin the chemical compounds of Mentha suaveolens(M. suaveolens) and Pinus halepensis(P. halepensis) essential oils(Eos) and evaluate their antioxidant and antibacterial activities. Methods: The chemical composition of P. halepensis and M. suaveolens EOs was determined by GC-MS analysis. The antioxidant activity was evaluated using DPPH,ABTS and FRAP assays. The antibacterial effect was tested against 6 bacterial strains using the well diffusion method and micro-dilution assay. Results: The major components of P. halepensis EOs were β-caryophyllene(28.04%),myrcene(23.81%) and α-pinene(12.02%). However,piperitenone oxid(56.28%),piperitenone(11.64%) and pulegone(6.16%) were the major components of M. suaveolens EOs. M. suaveolens EOs showed remarkable antioxidant activities compared with P. halepensis EOs,showing antioxidant capacity values of IC50=(64.76±2.24) μg/mL,IC50=(82.73±3.34) μg/mL,and IC50=(93.35±4.45) μg/mL,revealed by DPPH,ABTS and FRAP assays,respectively. However,P. halepensis EOs showed interesting antibacterial effects against all bacterial strains. The most sensible strains to P. halepensis EOs were Staphylococcus aureus [(34.00±0.50) mm],Listeria monocytogenes [(31.00±1.50)] mm and Proteus mirabilis [(29.00±2.25)mm]. Furthermore,the lowest minimal inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) values were revealed by P. halepensis EOs against Staphylococcus aureus [MIC=MBC=0.125%(v/v)] and Listeria monocytogenes [MIC=MBC=0.25%(v/v)]. Conclusions: P. halepensis and M. suaveolens EOs contain bioactive compounds that could have potential applications against bacterial infections and oxidative stress related diseases as well as for food conservation. However,further investigations are necessary to isolate and investigate the action mechanisms of these bioactive compounds.
文摘AIM:To examine the antiulcerogenic effects of various extracts of Mentha arvensis Linn on acid,ethanol and pylorus ligated ulcer models in rats and mice.METHODS:Various crude extracts of petroleum ether,chloroform,or aqueous at a dose of 2 g/kg po did not produce any signs or symptoms of toxicity in treated animals.In the pyloric ligation model oral administration of different extracts such as petroleum ether,chloroform and aqueous at 375 mg/kg po,standard drug ranitidine 60 mg/kg po and control group 1 Tween 80,5 mL/kg po to separate groups of Wister rats of either sex(n = 6) was performed.Total acidity,ulcer number,scoring,incidence,area,and ulcer index were assessed.RESULTS:There was a decrease in gastric secretion and ulcer index among the treated groups i.e.petroleum ether(53.4),chloroform(59.2),aqueous(67.0) and in standard drug(68.7) when compared to the negative control.In the 0.6 mol/L HCl induced ulcer model in rats(n = 6) there was a reduction in ulcerative score in animals receiving petroleum ether(50.5),chloroform(57.4),aqueous(67.5) and standard.drug(71.2) when compared to the negative control.In the case of the 90 ethanol-induced ulceration model(n = 6) in mice,there was a decrease in ulcer score in test groups of petroleum ether(53.11),chloroform(62.9),aqueous(65.4) and standard drug ranitidine(69.7) when compared to the negative control.It was found that pre-treatment with various extracts of Mentha arvensis Linn in three rat/mice ulcer models ie ibuprofen plus pyloric ligation,0.6 mol/L HCl and 90 ethanol produced significant action against acid secretion(49.3 ± 0.49 vs 12.0 ± 0.57,P < 0.001).Pre-treatment with various extracts of Mentha arvensis Linn showed highly-significant activity against gastric ulcers(37.1 ± 0.87 vs 12.0 ± 0.57,P < 0.001).CONCLUSION:Various extracts of Mentha arvensis Linn.375 mg/kg body weight clearly shows a protective effect against acid secretion and gastric ulcers in ibuprofen plus pyloric ligation,0.6 mol/L HCl induced and 90 ethanol-induced ulcer models.
基金Supported by the Innovative Scientific and Technological Talents Team Construction Project in Zhengzhou City(10CXTD148)
文摘[Objective] The research aimed to study the antibacterial action of Mentha spicata Linn extract in vitro.[Method] The cylinder-plate method was used to measure the inhibitory zone size of Mentha spicata Linn extract.The test-tube double dilution method was used to measure the minimal inhibitory concentration(MIC)of Mentha spicata Linn extract on the four kinds of animal pathogenic bacteria,which could determine the antibacterial effect.[Result] The decoction,alcohol extract and volatile oil of Mentha spicata Linn had the different antibacterial effects on the four kinds of animal pathogenic bacteria.Especially the inhibition effect on the pig staphylococcus was comparatively obvious.MIC of Mentha spicata Linn decoction I,alcohol extract III,volatile oil V and volatile oil VI were respectively 31.25,62.5,2.32 and 2.31 mg/ml.[Conclusion] The decoction,alcohol extract and volatile oil of Mentha spicata Linn had the certain antibacterial effect in vitro,and the extracts which were gained by the different extraction methods had the different inhibition effects on the experimental bacteria.
文摘The effects of plant growth regulators on peppermint (Mentha piperita) cultured in vitro were studied for the purpose of maximizing growth and essential oil production in micropropagated plants. The basal medium was experimentally supplemented with the auxin 4-indol-3-ylbutyric acid (IBA) and the cytokinin 6-benzylaminopurine (BAP) individually and in combination. Supplementation with BAP alone resulted in the highest values for root length, root dry weight, shoot length, and numbers of nodes, leaves, and ramifications. Treatment with IBA alone or with IBA + BAP resulted in a ~50% increase in shoot fresh weight. The production of secondary metabolites was affected only by the addition of cytokinin, which resulted in a ~40% increase in the total yield of essential oils (EOs). Similar trends were observed for yields of the major EO components (menthone, menthol, pulegone, and menthofuran). Our findings demonstrate that the application of growth regulators increases EO production and biomass concomitantly in an herbaceous species rich in commercially valuable terpenes.
文摘Mentha piperita L. (Peppermint) is a perennial glabrous and strongly scented herb belonging to family Lamiaceae. The plant is aromatic, stimulant and used for allaying nausea, headache and vomiting. Its oil is one of the most popular widely used essential oils in food products, cosmetics, pharmaceuticals, dental preparations, mouthwashes, soaps and alcoholic liquors. The antibacterial potential of six extracts from leaf, stem and root of Mentha piperita against pathogenic bacteria such as Bacillus subtilis, Streptococcus pneumonia, Staphylococcus aureus, Escherichia coli, Proteus vulgaris and Klebsiella pneumonia were evaluated by agar well diffusion method. The organic (ethanol, methanol, ethyl acetate, chloroform, hexane and petroleum ether) extracts of the leaves were found to possess strong antibacterial activity against a range of pathogenic bacteria. The ethyl acetate leaf extract of Mentha piperita showed pronounced inhibition than chloroform, petroleum ether and hexane. The leaf extract activity being more on Bacillus subtilis, Staphylococcus aureus and Proteus vulgaris than Escherichia coli, Streptococcus pneumonia and Klebsiella pneumonia. In the present study, we also evaluated the phytochemical analysis for the presence of various secondary metabolites. The analysis revealed the presence of alkaloids, flavonoids, steroids, tannins, and phenols.
文摘Purpose: Premature ovarian failure (POF) includes cessation of normal ovarian function before age 40, causing amenorrhea, menopausal symptoms and general health problems. Hormone replacement therapy (HRT) can be administered in order to prevent menopausal symptoms, osteoporosis and vascular complications. Due to the contraindications and side effects of HRT and the increasing demand for alternative therapeutic modalities, we used Mentha longifolia, which is known in the Iranian traditional medicine (ITM), as being able to cause menstrual bleeding in women with early amenorrhea and decreased FSH serum. Methods: In this pilot study, we report twenty seven amenorrheic women with POF who were referred to the Iranian traditional medicine clinic in Tehran and treated by Mentha longifolia. All those patients had high FSH levels and amenorrhea. They were in their fertility age, between 30 to 40 years old. They were asked to take a cup of herbal tea which was steeped with Mentha longifolia tea three times a day for 2 weeks in 3 menstural cycles. If the patients developed menstruation, the FSH serum level was measured immediately;otherwise they had an FSH measurement one month after the beginning of therapy. Results: Mean age of patients was 36.44 ± 1.5 years. Mean FSH level before treatment was 79.13 ± 19.17 mIU/ml, and after treatment was 27.83 ± 16.14 mIU/ml. In four patients who did not have any response, mean range of FSH was 82.37 ± 19.75 mIU/ml before treatment and afterwards it was 81.97 ± 21.52 mIU/ml. After treatment with herbal teas, there was a significant decrease in FSH (79.39 ± 19.17 to 27.83 ± 16.14 mIU/ml, p value < 0.001). All patient except four (14.81%) had mensturation after taking the herbal medicine. Those four patients showed no decrease in FSH level. More of the patients who used medicinal tea achieved regular monthly bleeding when followed for three cycles.
文摘Rumex abyssinica Jacq. (Polygonaceae) is locally used in management of allergies and female reproductive healthcare;whereas Mentha spicata L. (Lamiaceae) is used to treat gastrointestinal and respiratory distress, dandruff, halitosis and malaria in Uganda. Owing to the paucity of data on their safety profiles, this study evaluated the acute and sub-acute toxicities of 70% ethanolic leaf extracts of both plants in mice and Wistar albino rats. The oral acute toxicity of both plants was evaluated in Swiss mice of 7-8 weeks old (16-22 g) body weight and LD50 determined. Sub-acute toxicity was evaluated in Wistar albino rats (6 per group) at dose rates of 500, 1000 and 1500 mg/kg for 28 days. The LD50 of R. abyssinica and M. spicata in mice was 7727 mg/kg and 13,606 mg/kg body weight, respectively. General signs of toxicity due to large doses of both plants extract included hyperurination, abdominal muscle twitches and convulsions. In the sub-acute toxicity test, rats treated with both extracts did not exhibit any clinical signs of toxicity;no mortality and changes in body weight were observed. R. abyssinica did not cause significant changes in haematological indices, except a significant increase in HCT (p 0.05). However, a dose dependant significant decrease in HCT (p 0.05) and a significant increase (p 0.05) in the levels of WBC, LMY and MCHC were observed in rats treated with M. spicata extract. Biochemical test showed that both extracts caused a significant dose dependent increase (p 0.05) in levels of ALT and ALP. Marked increase in the levels of AST was also observed in rats treated with M. spicata extract. Of the two extracts, only rats treated with </
