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MEN1调控半胱氨酸代谢抑制肺癌生长的机制
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作者 王子名 王旭艳 +5 位作者 朱佳美 杨云巧 潘婷 陈腾祥 潘际刚 金帮明 《贵州医科大学学报》 2025年第12期1726-1739,共14页
目的探讨多发性内分泌肿瘤1型综合征(multiple endocrine neoplasia type 1,MEN1)关键基因MEN1调控半胱氨酸代谢在肺癌发展中的作用及机制。方法18只C57BL/6小鼠包含WT组(野生型作为对照组,n=6),KMS组[肺泡Ⅱ型上皮细胞(typeⅡalveolar ... 目的探讨多发性内分泌肿瘤1型综合征(multiple endocrine neoplasia type 1,MEN1)关键基因MEN1调控半胱氨酸代谢在肺癌发展中的作用及机制。方法18只C57BL/6小鼠包含WT组(野生型作为对照组,n=6),KMS组[肺泡Ⅱ型上皮细胞(typeⅡalveolar epithelial cells,AT2)特异性Men1敲除联合鼠类肉瘤病毒癌基因^(G12D)位点(kirsten rat sarcoma viral oncogene^(G12D),Kras^(G12D)),n=6]和KS组(AT2细胞Kras^(G12D)突变诱导,n=6);12只C57BL/6小鼠包含Men1-WT组(野生型作为对照组,n=6)和CKO组(Men1条件性敲除,n=6);NCI-H460细胞分为NC组(MEN1野生型)和KO组(MEN1敲除);A549细胞分为shLuc组(感染shLuc慢病毒)、shMEN1组(MEN1敲低)、vector组(感染vector慢病毒)和oeMEN1组(MEN1过表达);收集5位肺癌患者的肿瘤和癌旁组织,按各分期分为肿瘤组和癌旁组;苏木素-伊红(hematoxylin-eosin staining,HE)染色检测小鼠肿瘤发生情况;免疫组织化学(immunohistochemistry,IHC)检测KS和KMS小鼠肺组织中胱硫醚-β-合成酶(cystathionine-beta-synthase,CBS)和胱硫醚-γ-裂解酶(cystathionine gamma-lyase,CTH)蛋白表达;克隆形成检测半胱氨酸和MEN1对细胞增殖能力影响;流式细胞仪检测半胱氨酸和MEN1对肺癌细胞死亡影响;通过对硫化氢(hydrogen sulfide,H2S)产生量检测验证肺癌和MEN1对半胱氨酸合成代谢的影响;实时荧光定量PCR(quantitative real-time polymerase chain reaction,RT-qPCR)和Western blot检测MEN1敲除和半胱氨酸缺乏对CBS、CTH、溶质载体家族3成员2(solute carrier family 3 member 2,SLC3A2)及溶质载体家族7成员11(solute carrier family 7 member 11,SLC7A11)的mRNA和蛋白表达影响;染色质免疫共沉淀-实时荧光定量PCR(chromatin immunoprecipitation quantitative polymerase chain reaction,ChIP-qPCR)检测menin和组蛋白H3第4位赖氨酸三甲基化(histone H3 lysine 4 trimethylation,H3K4me3)与CBS和CTH启动子区域结合。结果MEN1缺失促进肺癌细胞增殖和克隆形成(P<0.05),加速Kras^(G12D)诱导肺癌小鼠的发病进程;半胱氨酸缺乏条件,MEN1缺失减少NCI-H460细胞死亡(P<0.01);MEN1缺失降低肺癌细胞中半胱氨酸水平(P<0.05),抑制转硫途径相关基因CBS和CTH的mRNA和蛋白表达(P<0.01),MEN1过表达促进CBS和CTH蛋白表达;人肺癌组织与癌旁组相比menin、CBS和CTH蛋白均低表达(P<0.05)。ChIP-qPCR结果显示menin和H3K4me3在CBS和CTH启动子高度富集,MEN1敲除显著降低CBS和CTH启动子区域H3K4me3的修饰水平(P<0.01)。结论MEN1是一个重要的肺癌抑制因子,通过H3K4me3激活CBS和CTH的转录,调控半胱氨酸代谢,从而抑制肺癌的发展。 展开更多
关键词 肺肿瘤 men1基因 半胱氨酸代谢 menIN蛋白 H3K4三甲基化修饰
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The Climate Change Influences on the Trees and a Life of Men in the Mekong River Delta-Remedial Actions
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作者 An Nguyen Thi Ngoc Trang Huynh Nguyen Thuy 《Journal of Environmental Science and Engineering(A)》 2025年第4期192-196,共5页
Mekong River Delta has many home-gardens,here,everybody organizes the tourisms.We observed the real situations and substances,evaluation,a choice at some households in the Mekong River Delta in order to have a purpose... Mekong River Delta has many home-gardens,here,everybody organizes the tourisms.We observed the real situations and substances,evaluation,a choice at some households in the Mekong River Delta in order to have a purpose of search,here,they have the home-gardens;the farmers plant fruit trees at the villages of provinces,that is a place which is influenced by the climate change.We went to the villages such as:Hiep Thanh village,Chau Thanh district,Long An province;Tan Phu village,Tan Phu Dong district,Tien Giang province;Tieu Can village,Tieu Can district,Tra Vinh province to observe the landscape(here 10 households for 1 village),and we took the sample to analyze.We knew the factors such as:drought,deficiency of water,salt water intrusion,flood.These factors influence the trees,assets,diseases,lives of the persons who stay here,and cause many damages.We compare many home-gardens having a climate change with the normal home-gardens.Thus,we propose the reasonable methods in order to fix the consequence and prevent the salt intrusion,flood,important damages…And we present some illustrations. 展开更多
关键词 Climate change trees men home-gardens
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盐酸美金刚胺前药MeN-1和MeN-2在人工胃肠液中的稳定性研究 被引量:4
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作者 肖瑶 曹俊涵 +2 位作者 赵书文 蒋德锡 张丹 《华西药学杂志》 CAS CSCD 北大核心 2011年第4期389-390,共2页
目的考察盐酸美金刚胺前药MeN-1和MeN-2在人工胃肠液中的稳定性。方法采用HPLC法测定盐酸美金刚胺前药MeN-1和MeN-2在人工胃液和人工肠液中的浓度,并比较两者的稳定性。结果 MeN-1在人工胃液中孵育3 h后,其相对含量为90%,但在人工肠液... 目的考察盐酸美金刚胺前药MeN-1和MeN-2在人工胃肠液中的稳定性。方法采用HPLC法测定盐酸美金刚胺前药MeN-1和MeN-2在人工胃液和人工肠液中的浓度,并比较两者的稳定性。结果 MeN-1在人工胃液中孵育3 h后,其相对含量为90%,但在人工肠液中降至约70%;而MeN-2在人工胃肠液中孵育1 h后,其相对含量均降至90%以下。结论在人工胃液中,MeN-1较MeN-2稳定,表明MeN-1更适于开发为口服制剂。 展开更多
关键词 盐酸美金刚胺 前药 men-1 men-2 人工胃液 人工肠液 稳定性 高效液相色谱法
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A pilot study on the correlation between dietary habits and osteoporosis in men living in the frigid regions of China
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作者 Yuqi Zhang Xiaohan Miao +5 位作者 Meng Guo Yizhen Nie Yi Zeng Chun Xu Lihong Jiang Jia Meng 《Frigid Zone Medicine》 2025年第4期206-216,共11页
Objective:To analyze the risk of osteoporosis among middle-aged men in the cold regions of China(Heilongjiang Province)and provide theoretical support for the early identification of high-risk populations.Methods:Bone... Objective:To analyze the risk of osteoporosis among middle-aged men in the cold regions of China(Heilongjiang Province)and provide theoretical support for the early identification of high-risk populations.Methods:Bone mineral density(BMD)data were collected from male subjects aged 50-65 who met the inclusion criteria at the physical examination center of a hospital in Harbin between August to December 2022.General clinical data and dietary information were obtained through face-to-face interviews using a dietary questionnaire survey.Results:The prevalence of osteoporosis and osteopenia was 14.38%and 52.06%,respectively,while normal bone mass accounted for 33.56%.Significant differences were observed among groups in smoking habits,sunlight exposure,exercise levels,and dietary patterns at each bone mass level.The BMD of the lumbar spine,femoral neck,and hip showed a negative correlation with the Dietary Inflammatory Index(DII)score.Multivariate logistic regression analysis revealed that smoking and a diet high in oil and salt were positively associated with the risk of osteoporosis.A pro-inflammatory diet was also positively correlated with osteoporosis risk,with individuals in this group being 7.723 times more likely to develop osteoporosis compared to those in the anti-inflammatory diet group.Conclusion:The high prevalence of osteoporosis and osteopenia observed in this study highlighted that osteoporosis is a significant and pressing issue among middle-aged men.Smoking,limited sunlight exposure,reduced physical activity,diets high in oil and salt,and pro-inflammatory diets were identified as major risk factors for bone loss.These factors are closely linked to the geography,climate,and cultural practices of cold regions in China.Primary healthcare in this region should focus on the screening and prevention of osteoporosis in middle-aged men by promoting smoking cessation,increased sunlight exposure,adequate vitamin D supplementation,regular physical activity,and adherence to a healthy diet to maintain bone health. 展开更多
关键词 OSTEOPOROSIS middle-aged men dietary patterns bone mineral density frigid regions risk factors
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A 10-year longitudinal analysis of the impact of demographic,lifestyle,and medical factors on semen qualities in men in a city in the midwestern region of the United States of America
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作者 Malik Scott Anaelena Rodriguez +3 位作者 Orry Marciano Rachel Nordgren Scott D Lundy Omer A Raheem 《Asian Journal of Andrology》 2025年第4期464-469,共6页
This study was to survey the relationship between semen values and demographics,comorbidities,and recreational substance use in a large cohort of adult men at the University of Chicago Medical Center Department of Uro... This study was to survey the relationship between semen values and demographics,comorbidities,and recreational substance use in a large cohort of adult men at the University of Chicago Medical Center Department of Urology(Chicago,IL,USA).We performed an analysis from January 2013 to December 2023 of semen samples obtained from adult patients at our institution and collected their demographics,comorbid medical conditions,and recreational substance use information.Patients were divided into categories of normozoospermia,oligozoospermia,and azoospermia on the basis of the 5th version of the World Health Organization(WHO)guidelines.Data were analyzed by univariate linear and logistic regression models,after which statistically significant variables were placed into multivariable models.Azoospermia and oligozoospermia were both associated with Caucasian or Black,Indigenous,and People of Color(BIPOC)race(both P<0.001),increasing age(P=0.005 and P<0.001,respectively),anemia(P<0.001 and P=0.02,respectively),lifetime tobacco use(both P<0.001),lifetime alcohol use(P=0.02 and P<0.001,respectively),and lifetime use of at least two recreational substances(P<0.001 and P=0.003,respectively)in multivariable models.Oligospermia was additionally associated with benign prostatic hyperplasia(BPH;P=0.003)in multivariable models.This study suggests that at-risk populations may benefit from additional early screening and workup for infertility. 展开更多
关键词 INFERTILITY men’s health metabolic diseases race factors recreational drug use
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A Study on Magical Realist Writing Techniques Used in Men of Maize
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作者 Jinglan Yu 《Journal of Contemporary Educational Research》 2025年第5期165-172,共8页
MiguelÁngel Asturias’work titled Men of Maize stands as a significant masterpiece within the realm of Latin American magical realism literature.It unfolds and discloses the profound and abiding antagonism that e... MiguelÁngel Asturias’work titled Men of Maize stands as a significant masterpiece within the realm of Latin American magical realism literature.It unfolds and discloses the profound and abiding antagonism that exists between Indian culture and modern capitalism by means of a prose narrative that is rendered in a poeticized fashion,replete with rich mythological symbols as well as complex social conflicts that are intricately interwoven.This paper undertakes an analysis across four distinct aspects,namely the backdrop or setting against which the work was created,the very definition along with the characteristic features that are emblematic of magical realism,the context or background within which it came into being and subsequently developed,and the specific and tangible manifestations of the magical realist writing techniques as they are exemplified in Men of Maize.The study that has been conducted reveals that Men of Maize manages to demonstrate the historical fissure or divide and the cultural antagonism that pervades Latin American society through the employment of techniques such as the clash of cultures,a non-traditional narrative structure that breaks away from the norm,and the symbolism associated with deities.Moreover,it simultaneously engages in a profound and incisive reflection upon the marginalization of traditional culture during the course of the modernization process.The creative output of Asturias is not solely a critique leveled at the social reality of Latin America;rather,it also represents an exploration of the common issues and challenges that are faced by mankind at large,thereby laying down an important and substantial foundation for the further development of magical realism literature. 展开更多
关键词 Magical realism men of Maize Techniques of magical realist writing
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MEN1基因与胃肠道胰腺神经内分泌肿瘤 被引量:4
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作者 叶必星 林琳 《国际消化病杂志》 CAS 2010年第6期365-367,379,共4页
MEN1基因为多发性内分泌腺肿瘤(MEN1)的易感基因,在调节基因的转录、维持基因的稳定、调控细胞的分裂和增殖方面起重要作用,而MEN1基因失活可能与胃肠道胰腺神经内分泌肿瘤(GEP-NET)发生机制有关。
关键词 胃肠道胰腺神经内分泌肿瘤 men1基因 menIN 分子机制
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Menin蛋白的生物学功能研究进展 被引量:2
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作者 华红伟 丁罡 《中国肿瘤》 CAS 2013年第8期656-660,共5页
Menin蛋白是MEN1基因的表达产物,MEN1是多发性内分泌肿瘤1型综合征(multiple endocrine neoplasia type1,MEN1)的关键致病基因之一。menin在细胞核中与其他关键转录因子相互作用,直接参与组蛋白甲基化修饰等基因表达调控过程。此外,meni... Menin蛋白是MEN1基因的表达产物,MEN1是多发性内分泌肿瘤1型综合征(multiple endocrine neoplasia type1,MEN1)的关键致病基因之一。menin在细胞核中与其他关键转录因子相互作用,直接参与组蛋白甲基化修饰等基因表达调控过程。此外,menin在基因组稳定、细胞分裂增殖及细胞周期调控中都发挥着重要的作用,在肿瘤的发生和发展中扮演着重要的角色。全文对menin蛋白的生物学功能进展进行综述。 展开更多
关键词 menIN蛋白 men1 生物学功能
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Total Energy Expenditure of 16 Chinese Young Men Measured by the Doubly Labeled Water Method 被引量:4
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作者 ZHUO Qin SUN Rui +5 位作者 GOU Ling Yan PIAO Jian Hua LIU Jian Min TIAN Yuan ZHANG Yu Hui YANG Xiao Guang 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2013年第6期413-420,共8页
Objective Doubly labeled water (DLW) method is the gold standard for measuring total energy expenditure (TEE). We used this method to measure TEE in Chinese young men. Methods Sixteen healthy young men age 23±... Objective Doubly labeled water (DLW) method is the gold standard for measuring total energy expenditure (TEE). We used this method to measure TEE in Chinese young men. Methods Sixteen healthy young men age 23±1 recruited. TEE was measured by the DLW method, by indirect calorimetry. We also conducted 24-h estimate energy requirements of the subjects. years with body mass index 22.0±1.4 kg/m2 were and basal energy expenditure (BEE) was determined activity, energy balance and factorial approach to Results TEE of subjects by DLW method was 9.45±0.57 M J/day (2258±180 kcal/day). The 24-h activity was 10.80±0.33 M J/day (2582±136 kcal/day). The energy requirement, derived from energy balance observations, was 9.93±1.32 M J/day (2373±315 kcal/day). The BEE of 6.65±0.28 M J/day (1589±67 kcal/day), calculated by the adjusted Schofield equation, was significantly higher (P〈O.O01) than that measured by indirect calorimetry, 5.99-±0.66 M J/day (1433-±158 kcal/day). The TEE derived from the factorial approach was 10.31-±0.43 M J/day (2463±104 kcal/day). Conclusion The TEE of Chinese young men measured by the DLW method was about 10% lower than the current recommended nutrient intake (RNI), suggesting that the RNI for Chinese men maybe overestimated. Further studies are warranted to determine the value of the estimated energy requirement. 展开更多
关键词 Doubly labeled water Total energy expenditure Recommended nutrient intakes Estimatedenergy requirement Chinese young men
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Fhe reference values for semen parameters of 1213 ertile men in Guangdong Province in China 被引量:3
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作者 Yun-Ge Tang 《Asian Journal of Andrology》 SCIE CAS CSCD 2015年第2期298-303,I0011,共7页
Semen samples were collected from 1213 fertile men whose partners had a time-to-pregnancy (TTP) _〈12 months in Guangdong Province in Southern China, and semen parameters including semen volume, sperm concentration,... Semen samples were collected from 1213 fertile men whose partners had a time-to-pregnancy (TTP) _〈12 months in Guangdong Province in Southern China, and semen parameters including semen volume, sperm concentration, total counts, motility, and morphology were evaluated according to the World Health Organization (WHO) 2010 guideline. All semen parameters analyzed were normal in ~62.2% of the total samples, whereas 37.8% showed at least one of the semen parameters below normal threshold values. The fifth centiles (with 95% confidence intervals) were 1.3 (1.2-1.5) ml for semen volume, 20 x 106 (18x106- 20x106) m1-1 for sperm concentration, 40 x 106 (38x106-44x106) per ejaculate for total sperm counts, 48% (47%-53%) for vitality, 39% (36%-43%) for total motility, 25% (23%-27%) for sperm progressive motility, 5.0% (4%,5%) for normal morphology. The pH values ranged from 7.2 to 8.0 with the mean ~ standard deviation at 7.32 ~ 0.17. No effects of age and body mass index were found on semen parameters. Occupation, smoking and alcohol abuse, varicocele appeared to decrease semen quality. Sperm concentration, but not sperm morphology, is positively correlated with TTP, whereas vitality is negatively correlated with TTP. Our study provides the latest reference values for the semen parameters of Chinese fertile men in Guangdong Province, which are close to those described in the new WHO guidelines (5th Edition). 展开更多
关键词 Chinese fertile men semen parameters semen quality time-to-pregnancy
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Menin在翼状胬肉中的异常表达
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作者 王华 刘祖国 +3 位作者 夏晓波 刘双珍 赵丽蓓 王平宝 《中国现代医学杂志》 CAS CSCD 北大核心 2011年第19期2265-2268,共4页
目的探讨menin蛋白在初发性翼状胬肉组织中的表达。方法采用免疫组织化学染色和Western Blotting方法对30例初发性翼状胬肉组织进行menin蛋白的检测,并与26例正常人结膜组织进行对照。结果 Menin蛋白在正常人结膜上皮细胞和结膜基质血... 目的探讨menin蛋白在初发性翼状胬肉组织中的表达。方法采用免疫组织化学染色和Western Blotting方法对30例初发性翼状胬肉组织进行menin蛋白的检测,并与26例正常人结膜组织进行对照。结果 Menin蛋白在正常人结膜上皮细胞和结膜基质血管内皮细胞的细胞核内强阳性表达;Menin蛋白在翼状胬肉的结膜上皮细胞和基质血管内皮细胞的细胞浆内表达明显下调,弱阳性表达不再定位于细胞核内。结论 Menin蛋白表达异常可能在促进翼状胬肉上皮细胞和基质血管内皮细胞过度增殖中发挥重要的调控作用。 展开更多
关键词 menIN men1 翼状胬肉
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Development of a Social Activities Scale for Community-Dwelling Older Men Requiring Support in Japan 被引量:1
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作者 Michiyo Hirano Kazuko Saeki Izumi Ueda 《Health》 2018年第1期1-12,共12页
Aim: We developed a scale to measure the social activities of community-dwelling older men requiring support. Methods: The participants were a group of 134 men, ≥65 years old, who required support and were living in ... Aim: We developed a scale to measure the social activities of community-dwelling older men requiring support. Methods: The participants were a group of 134 men, ≥65 years old, who required support and were living in Hokkaido, Japan. An anonymous questionnaire was administered through individual interviews. Valid responses were obtained from 121/134 interviewees. The construct validity of the resulting scale was assessed by exploratory factor analysis (EFA). Criterion-related validity was tested with Spearman’s rank correlation test based on the Social Activities Index for Elderly People (SAI-E). Reliability was assessed by Cronbach’s alpha. Results: A Social Activities Scale for Community-dwelling Older Men Requiring Support (SASOMS) scale was created, comprised of the following three subscales: daily interactions with familiar people;intimate relationships with family members;and interactions with others through activity programs (e.g., exercise, games, recreation, etc.). The created SASOMS scale correlated with the SAI-E (r = 0.557), and its criterion-related validity was confirmed. The alpha coefficient of the new scale was 0.791, and its internal consistency was confirmed. Conclusions: The reliability and validity of the developed SASOMS scale was confirmed, demonstrating that it can be used to assess social activities specifically in older men requiring support. Our results suggest that the scale can be used effectively by care providers who support older men requiring care. The usability of the SASOMS should continue to be improved, and it is necessary to verify its validity in longitudinal studies. 展开更多
关键词 Instrument DEVELOPmenT OLDER men’s Health SOCIAL ACTIVITIES SCALE
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Structure of Macro- and Trace Elements Status Observed in Residents of Magadan Town
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作者 Lugovaya Elena Stepanova Evgenia 《Journal of Life Sciences》 2014年第9期794-797,共4页
The authors aimed the study at examining macro- and trace elements content in elderly people of the 1 period of middle age (21-35) residing in Magadan town to assess the content of 25 trace elements in hair samples ... The authors aimed the study at examining macro- and trace elements content in elderly people of the 1 period of middle age (21-35) residing in Magadan town to assess the content of 25 trace elements in hair samples on individual and population levels by atom-emission spectrometry with inductively-bonded argon plasma. It turned out that the majority of surveyed subjects were characterized by deformation of element status demonstrated by excess and deficit of the basic essential elements of different degrees of manifestation. Deficit of Co (68% of surveyed people), Mg (67%), Ca (64%), Cu (47%), K (46%), Zn (44%), I (41%), Na (33%), P (27%) and Se (24%) was typical for female subjects as well as excess of Si (28%), Zn (22%) and P (20%). Male residents were characterized by deficit of Co (81%), Ca (66%), Mg (65%), I (50%), Cu (37%), K and Na (27%) and excess of Zn (41%). In individual cases it was noted excess concentration of nominally essential and toxic elements in both examined groups. They were: A1, As, Cd, Li, Ni, Pb, Sn, V and B. While analyzing of medians of chemical concentration it was stated reliable sex-related differences (P 〈 0.05): content of Ca, Mg and Mn was higher in hair samples of women, and content of As, B, Cd, Cr, K, Li, Na, P, Pb and V, on the contrary, was higher in male samples. 展开更多
关键词 Macro- and trace elements misbalance the North men women.
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[Co(3,3-tri)(men)Cl][ZnCl_4]和[Co(3,3-tri)(cmen)Cl][ZnCl_4]两体系配合物的合成及两经式异构体的晶体结构 被引量:1
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作者 毛明毅 薛赛凤 +5 位作者 陈铅 陶朱 祝黔江 罗绪强 周忠远 周向葛 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 北大核心 2002年第4期420-426,共7页
分别合成了 [Co(3, 3-tri)(men)Cl][ZnCl4]、[Co(3, 3-tri)(cmen)Cl][ZnCl4] (3, 3-tri = N-(3-胺基丙基)-1, 3-丙二胺,men = N-甲基乙二胺,cmen = 1, 2-二胺基-丙烷) 2体系的部分配合物异构体,用单晶 X-射线衍射分析方法解析了2异构体... 分别合成了 [Co(3, 3-tri)(men)Cl][ZnCl4]、[Co(3, 3-tri)(cmen)Cl][ZnCl4] (3, 3-tri = N-(3-胺基丙基)-1, 3-丙二胺,men = N-甲基乙二胺,cmen = 1, 2-二胺基-丙烷) 2体系的部分配合物异构体,用单晶 X-射线衍射分析方法解析了2异构体的晶体结构。其中 [Co(3, 3-tri)(men)Cl][ZnCl4] 体系的一异构体Ⅰ的化学简式为 CoCl(C9H27N5)ZnCl4,晶体属正交晶系,空间群 Pca21,a = 16.788(2),b = 7.964(1),c = 14.416(2) 牛琕 =1927.3(4) ?,Dc = 1.747 g/cm3,Z = 4,F(000) = 1032,Mr = 506.91,R = 0.0352,wR =0.0935;[Co(3, 3-tri)(cmen)Cl]2+ 体系的一异构体Ⅱ的化学简式为 CoCl(C9H27N5)ZnCl4稨2O, 晶体属三斜晶系,空间群 P ,a = 9.511(3), b = 9.972(3),c = 11.694(3) 牛琣 = 68.367(5),b = 85.196(6),?= 86.580(5),V = 1026.9(5)?3,Dc = 1.698 g/cm3,Z = 2,F(000) = 536,Mr = 524.92,R = 0.0494,wR = 0.1180。两异构体中 Co3+ 为六配位,晶胞中对映体比例均为1:1。在配合物异构体Ⅰ和Ⅱ中,三元胺以经式排布,三元胺配体(3, 3-tri)仲胺上的氢相对于Cl分别处于顺位(syn-)和反位(anti-);二元胺配体氮(或邻位碳)取代的胺基氮原子(N*)与三元胺配体中的仲氮原子分别处于对位(trans(N*))和邻位(cis(N*))。 展开更多
关键词 [Co(3 3-tri)(men)C1][ZnCl4] [Co(3 3-tri)(cmen)Cl][ZnCl4] 两经式异构体 钴(Ⅲ)配合物 晶体结构 三元胺配体
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玫瑰黄链霉菌Men-myco-93-63抗南方根结线虫相关酶活性及其防效 被引量:11
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作者 张艳杰 魏学军 +6 位作者 寇宏达 乔丹娜 冀红柳 李爱霞 赤国彤 李亚宁 刘大群 《西北农业学报》 CAS CSCD 北大核心 2013年第4期184-190,共7页
根据雌虫会阴花纹特征将采集地河北省定州市西南合村黄瓜棚的根结线虫鉴定为南方根结线虫,并研究对多种植物病原菌具有较强抑制作用的玫瑰黄链霉菌Men-myco-93-63对南方根结线虫的防效及其产生的与防治线虫相关的酶。结果表明,Men-myco-... 根据雌虫会阴花纹特征将采集地河北省定州市西南合村黄瓜棚的根结线虫鉴定为南方根结线虫,并研究对多种植物病原菌具有较强抑制作用的玫瑰黄链霉菌Men-myco-93-63对南方根结线虫的防效及其产生的与防治线虫相关的酶。结果表明,Men-myco-93-63发酵液对根结线虫二龄幼虫的校正致死率达到100%,粗酶液对线虫的校正致死率达到94.87%。Men-myco-93-63具有较强的胶原蛋白酶产生能力,也可产生丝氨酸蛋白酶和蛋白酶,发酵液中蛋白酶的活力达到473.363 7U/mL。推测胶原蛋白酶、蛋白酶、丝氨酸蛋白酶等酶类在该生防菌对南方根结线虫的防治中起重要作用。温室盆栽试验结果表明,玫瑰黄链霉菌Men-myco-93-63发酵液及其固体发酵物对根结线虫均有防效;其中,发酵液5倍稀释液对根结线虫的防效最好,达到39.99%。 展开更多
关键词 南方根结线虫 玫瑰黄链霉菌men-MYCO-93-63 蛋白酶 防治效果
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玫瑰黄链霉菌Men-myco-93-63发酵液对黄瓜白粉病抗性的影响 被引量:13
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作者 郭敬华 孟庆芳 +4 位作者 李亚宁 杨文香 张汀 周锟 刘大群 《华北农学报》 CSCD 北大核心 2007年第B08期1-4,共4页
药效试验表明,玫瑰黄链霉菌Men-myco-93-63发酵液对黄瓜白粉病有良好的防效,其中发酵液保护作用的防效为88.63%,治疗作用的防效为82.26%,表明该发酵液对黄瓜白粉病的防治既有保护作用又有治疗作用。通过对喷施发酵液后黄瓜叶片内的抗病... 药效试验表明,玫瑰黄链霉菌Men-myco-93-63发酵液对黄瓜白粉病有良好的防效,其中发酵液保护作用的防效为88.63%,治疗作用的防效为82.26%,表明该发酵液对黄瓜白粉病的防治既有保护作用又有治疗作用。通过对喷施发酵液后黄瓜叶片内的抗病相关酶的活性的测定发现,喷施Men-myco-93-63发酵液后黄瓜叶片内POD,CAT,PAL,PPO等酶的活性在一定时间内有明显上升的趋势。 展开更多
关键词 链霉菌men-myco-93-63 发酵液 黄瓜白粉病 酶活性
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玫瑰黄链霉菌Men-myco-93-63产抗生素发酵培养基的优化 被引量:5
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作者 韩兴 刘亚南 +2 位作者 甄丹妹 刘大群 赤国彤 《江苏农业科学》 北大核心 2017年第10期100-103,共4页
以玫瑰黄链霉菌Men-myco-93-63为发酵菌株,采用Plackett-Burman试验设计、最陡爬坡试验、中心复合试验设计和响应面分析法,以抗生素产量为响应值,对其发酵培养基进行优化。首先,采用Plackett-Burman设计筛选出具有正显著效应的花生饼粉... 以玫瑰黄链霉菌Men-myco-93-63为发酵菌株,采用Plackett-Burman试验设计、最陡爬坡试验、中心复合试验设计和响应面分析法,以抗生素产量为响应值,对其发酵培养基进行优化。首先,采用Plackett-Burman设计筛选出具有正显著效应的花生饼粉和玉米浆。其次,通过最陡爬坡试验逼近最大响应区域。最后,采用中心复合试验对这2个因素进行优化。最终确定发酵的最适培养基含量配方为葡萄糖2%,可溶性淀粉1%,花生饼粉4.96%,玉米浆2.66%,氯化钠0.4%,碳酸钙0.3%,磷酸二氢钾0.02%。优化后发酵培养基抗生素产量为2 030.6 mg/L,比优化前的771.9 mg/L提高了1.63倍。 展开更多
关键词 玫瑰黄链霉菌men—myco-93—63 发酵 抗生素 响应面分析
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嗜铬细胞瘤SDHD和MEN1基因突变与杂合性缺失研究 被引量:1
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作者 孙海燕 苏颋为 +5 位作者 崔斌 姜蕾 李小英 宁光 张军妮 王卫庆 《上海交通大学学报(医学版)》 CAS CSCD 北大核心 2006年第1期22-24,共3页
目的探讨抑癌基因SDHD和MEN1的突变与杂合性缺失,在嗜铬细胞瘤(PCC)发病机制中的作用。方法采用DNA直接测序法对SDHD和MEN1基因的全部外显子及其侧翼进行分析,寻找基因突变。选用荧光标记11q13区-23区的8个多态性微卫星位点对37例PCC进... 目的探讨抑癌基因SDHD和MEN1的突变与杂合性缺失,在嗜铬细胞瘤(PCC)发病机制中的作用。方法采用DNA直接测序法对SDHD和MEN1基因的全部外显子及其侧翼进行分析,寻找基因突变。选用荧光标记11q13区-23区的8个多态性微卫星位点对37例PCC进行杂合性缺失频率检测。分析上述检测结果与临床资料的关系。结果在37例PCC肿瘤组织中未发现SDHD和MEN1基因突变。发现3种SDHD基因内含子改变(15519972c/g,15521745c/t,15520001c/a),2种MEN1基因内含子改变(9879384a/g,9878352C/T)。SDHD所在区域11q23的杂合性缺失频率为30.8%。MEN1所在区域11q13的杂合性缺失频率为26.9%。结论PCC中未发现SDHD和MEN1基因突变;发现一定比例的11q13区-23区域的杂合性缺失,提示在该区域可能存在其他的抑癌基因参与PCC的发病机制。 展开更多
关键词 SDHD men1 杂合性缺失 突变 嗜铬细胞瘤
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玫瑰黄链霉菌Men-myco-93-63营养型片剂的研制及应用 被引量:5
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作者 冯龙 魏娇洋 +4 位作者 陈英化 李亚宁 张汀 刘大群 赤国彤 《河北农业大学学报》 CAS CSCD 北大核心 2014年第5期77-81,88,共6页
为了研制出一种防治棉花黄萎病的玫瑰黄链霉菌Men-myco-93-63营养型片剂,本试验利用单因素和双因素方差分析法筛选其氮源、碳源和粘合剂,并在田间试验评价该片剂对棉花黄萎病的防效。结果表明:片剂中载体硅藻土与玫瑰黄链霉菌Men-myco-9... 为了研制出一种防治棉花黄萎病的玫瑰黄链霉菌Men-myco-93-63营养型片剂,本试验利用单因素和双因素方差分析法筛选其氮源、碳源和粘合剂,并在田间试验评价该片剂对棉花黄萎病的防效。结果表明:片剂中载体硅藻土与玫瑰黄链霉菌Men-myco-93-63种子液最适质量体积比为2∶1,碳源为蔗糖,浓度15%;氮源为花生饼粉,浓度为15%;粘合剂聚乙二醇6000或玉米淀粉。制备的片剂重量差异符合标准,含菌量3×107cfu/g,含水量2%,杂菌率为1%,4℃真空条件下保存120d,菌含量仍能达到2×105 cfu/g。Men-myco-93-63片剂1.0g/株对棉花黄萎病的防效达到88.66%,皮棉产量达到2245.50kg/km2,明显优于其它对照药剂。生物农药片剂作为一种新农药剂型,具有很高的应用潜力。 展开更多
关键词 玫瑰黄链霉菌men-MYCO-93-63 片剂 双因素方差分析法 田间试验
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玫瑰黄链霉菌Men-myco-93-63负调控基因nsdAmgh的克隆及序列分析 被引量:4
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作者 沈凤英 刘力强 +2 位作者 吴伟刚 李亚宁 刘大群 《华北农学报》 CSCD 北大核心 2009年第5期77-80,共4页
nsdA(Negative regulator of Streptomyces differentiation)基因是天蓝色链霉菌中发现的与抗生素合成相关的负调控基因。根据天蓝色链霉菌A3(2)的序列设计引物扩增玫瑰黄链霉菌Men-myco-93-63菌株中的该基因,经测序和序列分析表明,由... nsdA(Negative regulator of Streptomyces differentiation)基因是天蓝色链霉菌中发现的与抗生素合成相关的负调控基因。根据天蓝色链霉菌A3(2)的序列设计引物扩增玫瑰黄链霉菌Men-myco-93-63菌株中的该基因,经测序和序列分析表明,由保守序列引物nsdA-2R和nsdA-2L克隆到的Men-myco-93-63菌株中的该基因800 bp片段与已知nsdA基因核苷酸保守区域序列同源性达到99%,由全长序列扩增引物nsdA WZ-R和nsdA WZ-L克隆到的该基因包含一个完整的开放阅读框,共编码369个氨基酸,与变铅青链霉菌和天蓝色链霉菌A3(2)中的nsdA基因的核苷酸序列同源性达到100%,氨基酸序列同源性达到99%。该全长基因命名为nsdAmgh,其在玫瑰黄链霉菌Men-myco-93-63中的发现为阻断该负调控基因以期获得抗生素高产工程菌株提供了重要依据。 展开更多
关键词 玫瑰黄链霉菌men-MYCO-93-63 nsdA基因 克隆 序列分析
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