BACKGROUND ATP-binding cassette subfamily B member 4(ABCB4)deficiency is associated with cholestatic liver disease primarily because of missense mutations,and many variants remain unidentified.Here,we validate the pat...BACKGROUND ATP-binding cassette subfamily B member 4(ABCB4)deficiency is associated with cholestatic liver disease primarily because of missense mutations,and many variants remain unidentified.Here,we validate the pathogenicity and mechanism of ABCB4 variants in clinical and in vitro trials,hypothesizing that these variants are responsible for impaired biliary function and contribute to the development of cholestatic liver diseases.AIM To clarify the functional features and pathogenicity of ABCB4 variants.METHODS Clinical data were collected from five patients with cholestatic liver disease that was initially not detected by routine examinations.Later,whole-exome sequencing confirmed ABCB4 variants and the patients were treated from January 2017 to December 2023.Pathogenic mechanisms were analyzed using bioinformatics tools,and a cell model in vitro was established to investigate ABCB4 mRNA expression,multidrug resistance protein 3(MDR3)expression,cellular localization,and phosphatidylcholine secretion.Results were compared using Student's t-tests.RESULTS Five missense variants(c.1757T>A,c.1865G>A,c.2362C>T,c.2777C>T and c.3250C>T),one intron variant(c.537-32G>T),and one synonymous(c.C504T)variant were identified.Three of the five patients had various degrees of cholestasis,two presented with liver cirrhosis,and all had elevated gamma-glutamyl transferase.Three of the four patients who underwent a liver biopsy had bile duct dilation,and one had gallstones.Two of the four patients had normal and reduced MDR3 immunohistochemical levels.Bioinformatic analysis indicated that these variants were likely pathogenic except c.C504T variant.None of the missense variants influenced subcellular MDR3 Localization in vitro.However,the c.1865G>A variant significantly decreased ABCB4 mRNA values,and all missense variants down-regulated phosphatidylcholine secretion.CONCLUSION This study uncovered new ABCB4 variants and emphasized the pathogenic potential of specific variants.The findings from five patients provided insight into the pathogenic mechanisms underlying ABCB4-related diseases.展开更多
Toll-like receptor （TLR）-mediated inflammatory response could negatively affect bone metabolism. In this study, we determined how osteogenesis is regulated during inflammatory responses that are downstream of TLR si...Toll-like receptor （TLR）-mediated inflammatory response could negatively affect bone metabolism. In this study, we determined how osteogenesis is regulated during inflammatory responses that are downstream of TLR signaling. Human primary osteoblasts were cultured in collagen gels. Pam3CSK4 （P3C） and Escherichia coli lipopolysaccharide （EcLPS） were used as TLR2 and TLR4 ligand respectively. Porphyromonas gingivalis LPS having TLR2 activity with either TLR4 agonism （Pg1690） or TLR4 antagonism （Pg1449） and mutant E. coli LPS （LPxE/LPxF/WSK） were used. IL-lp, SH2-containing inositol phosphatase-1 （SHIP1） that has regulatory roles in osteogenesis, alkaline phosphatase and mineralization were analyzed. 3α-Aminocholestane （3AC） was used to inhibit SHIP1. Our results suggest that osteoblasts stimulated by P3C, poorly induced IL-1β but strongly upregulated SHIP1 and enhanced osteogenic mediators. On the contrary, EcLPS significantly induced IL-1β and osteogenic mediators were not induced. While Pg1690 downmodulated osteogenic mediators, Pg1449 enhanced osteogenic responses, suggesting that TLR4 signaling annuls osteogenesis even with TLR2 activity. Interestingly, mutant E. coli LPS that induces weak inflammation upregulated osteogenesis, but SHIP1 was not induced. Moreover, inhibiting SHIP1 significantly upregulated TLR2-mediated inflammatory response and downmodulated osteogenesis. In conclusion, these results suggest that induction of weak inflammatory response through TLR2 （with SHIP1 activity） and mutant TLR4 ligands could enhance osteogenesis.展开更多
In this paper, the admittance function between seafloor undulations and vertical gravity gradient anomalies was derived. Based on this admittance function, the bathymetry model of 1 minute resolution was predicted fro...In this paper, the admittance function between seafloor undulations and vertical gravity gradient anomalies was derived. Based on this admittance function, the bathymetry model of 1 minute resolution was predicted from vertical gravity gradient anomalies and ship soundings in the experimental area from the northwest Pacific. The accuracy of the model is evaluated using ship soundings and existing models, including ETOPO1, GEBCO, DTU10 and V15.1 from SIO. The model＇s STD is 69. 481m, comparable with V15.1 which is generally believed to have the highest accuracy.展开更多
Through regulating target genes via the mechanisms of posttranscriptional cleavage or translational repression, plant miRNAs involve diverse biological processes associating with plant growth, development, and abiotic...Through regulating target genes via the mechanisms of posttranscriptional cleavage or translational repression, plant miRNAs involve diverse biological processes associating with plant growth, development, and abiotic stress responses, in this study, we functionally characterized TaMIR1119, a miRNA family member of wheat （Triticum aestivum）, in regulating the drought adaptive response of plants. TaMIR1119 putatively targets six genes categorized into the functional classes of transcriptional regulation, RNA and biochemical metabolism, trafficking, and oxidative stress defense. Upon simulated drought stress, the TaMIR1119 transcripts abundance in roots was drastically altered, showing to be upregulated gradually within a 48-h drought regime andthat the drought-induced transcripts were gradually restored along with a 48-h recovery treatment. In contrast, most miRNA target genes displayed reverse expression patterns to TaMIR1119, exhibiting a downregulated expression pattern upon drought and whose reduced transcripts were re-elevated along with a normal recovery treatment. These expression analysis results indicated that TaMIR1119 responds to drought and regulates the target genes mainly through a cleavage mechanism. Under drought stress, the tobacco lines with TaMIR1119 overexpression behaved improved phenotypes,, showing increased plant biomass, photosynthetic parameters, osmolyte accumulation, and enhanced antioxidant enzyme （AE） activities relative to wild type. Three AE genes, NtFeSOD, NtCAT1;3, and NtSOD2,1, encoding superoxide dismutase （SOD）, catalase （CAT）, and peroxidase （POD） proteins, respectively, showed upregulated expression in TaMIR1119 overexpression lines, suggesting that they are involved in the regulation of AE activities and contribution to the improved cellular reactive oxygen species （ROS） homeostasis in drought-challenged transgenic lines. Our results indicate that TaMIR1119 plays critical roles in regulating plant drought tolerance through transcriptionally regulating the target genes that modulate osmolyte accumulation, photosynthetic function, and improve cellular ROS homeostasis of plants.展开更多
AIM： To investigate those associations using data of the population-based Study of Health in Pomerania. METHODS： A study population of 3 749 residents aged 20-79 years without previously diagnosed thyroid disease wa...AIM： To investigate those associations using data of the population-based Study of Health in Pomerania. METHODS： A study population of 3 749 residents aged 20-79 years without previously diagnosed thyroid disease was available for analyses. Serum TSH was used to assess thyroid function. Cholelithiasis was defined by either a prior history of cholecystectomy or the presence of gallstones on ultrasound. Logistic regression was performed to analyze independent associations between thyroid function and cholelithiasis. RESULTS： There were 385 persons （10.3%） with low （〈0.3 mIU/L）, 3 321 persons （88.6%） with normal and 43 persons （1.2%） with high serum TSH levels （〉3 mIU/L）. The proportion of cholelithiasis among males and females was 14.4% and 25.3%, respectively. Among males, there was an independent relation between high serum TSH and cholelithiasis （OR 3.77; 95%-CI 1.06-13.41; P〈0.05）. Also among males, there was a tendency towards an elevated risk of cholelithiasis in persons with low serum TSH （OR 1.40; 95%-CI 0.96-2.02; P= 0.07）. In the female population, no such relation was identified. CONCLUSION： There is an association between thyroid and gallstone disease with a gender-specific relation between hypothyroidism and cholelithiasis.展开更多
A domain decomposition and matching method in the time-domain is outlined for simulating the motions of ships advancing in waves. The flow field is decomposed into inner and outer domains by an imaginary control surfa...A domain decomposition and matching method in the time-domain is outlined for simulating the motions of ships advancing in waves. The flow field is decomposed into inner and outer domains by an imaginary control surface, and the Rankine source method is applied to the inner domain while the transient Green function method is used in the outer domain. Two initial boundary value problems are matched on the control surface. The corresponding numerical codes are developed, and the added masses, wave exciting forces and ship motions advancing in head sea for Series 60 ship and S175 containership, are presented and verified. A good agreement has been obtained when the numerical results are compared with the experimental data and other references. It shows that the present method is more efficient because of the panel discretization only in the inner domain during the numerical calculation, and good numerical stability is proved to avoid divergence problem regarding ships with flare.展开更多
The PDFs(probability density functions) and probability of a ship rolling under the random parametric and forced excitations were studied by a semi-analytical method. The rolling motion equation of the ship in random ...The PDFs(probability density functions) and probability of a ship rolling under the random parametric and forced excitations were studied by a semi-analytical method. The rolling motion equation of the ship in random oblique waves was established. The righting arm obtained by the numerical simulation was approximately fitted by an analytical function. The irregular waves were decomposed into two Gauss stationary random processes, and the CARMA(2, 1) model was used to fit the spectral density function of parametric and forced excitations. The stochastic energy envelope averaging method was used to solve the PDFs and the probability. The validity of the semi-analytical method was verified by the Monte Carlo method. The C11 ship was taken as an example, and the influences of the system parameters on the PDFs and probability were analyzed. The results show that the probability of ship rolling is affected by the characteristic wave height, wave length, and the heading angle. In order to provide proper advice for the ship’s manoeuvring, the parametric excitations should be considered appropriately when the ship navigates in the oblique seas.展开更多
The effect of coupling between sloshing and ship motions in the evaluation of slosh-induced interior pressures is studied. The coupling between sloshing loads and ship motions is modelled through a hybrid algorithm wh...The effect of coupling between sloshing and ship motions in the evaluation of slosh-induced interior pressures is studied. The coupling between sloshing loads and ship motions is modelled through a hybrid algorithm which combines a potential flow solution based on transient Green function for the external ship hydrodynamics with a viscous flow solution based on a multiphase interface capturing volume of fluid(VOF) technique for the interior sloshing motion. The coupled algorithm accounts for full nonlinear slosh forces while the external forces on the hull are determined through a blended scheme of linear radiationdiffraction with nonlinear Froude-Krylov and restoring forces. Consideration of this level of nonlinearities in ship motions is found to have non-negligible effects on the slosh-coupled responses and slosh-induced loads. A scheme is devised to evaluate the statistical measure of the pressures through long-duration simulation studies in extreme irregular waves. It is found that coupling significantly influences the tank interior pressures, and the differences in the pressures between coupled and uncoupled cases can be as much as 100% or more. To determine the RAO over the frequency range needed for the simulation studies in irregular waves, two alternative schemes are proposed, both of which require far less computational time compared to the conventional method of finding RAO at each frequency, and the merits of these are discussed.展开更多
Adopting complex number theory, a mathematic model of Green function is built for two dimension free water surface, and an analytic expression of Green function is obtained by introducing two parameters. The intrinsic...Adopting complex number theory, a mathematic model of Green function is built for two dimension free water surface, and an analytic expression of Green function is obtained by introducing two parameters. The intrinsic properties of Green function are discussed on vertical line and horizontal line. At last, the derivation expression of Green function is obtained from the formula of Green function.展开更多
A robust H∞sampled-data stabilization problem for nonlinear dynamic positioning(DP) ships with Takagi-Sugeno(T-S) fuzzy models is discussed in this paper. Input delay approach is used to convert the sampleddata DP sh...A robust H∞sampled-data stabilization problem for nonlinear dynamic positioning(DP) ships with Takagi-Sugeno(T-S) fuzzy models is discussed in this paper. Input delay approach is used to convert the sampleddata DP ship system to a fuzzy system with time-varying delay. Adequate conditions are derived to determine the system's asymptotical stability and achieve H∞performance via Lyapunov stability theorems. Then, the fuzzy sampled-data controller is obtained by analyzing the stabilization condition. Simulation result shows that the proposed method and the designed controller for a DP ship are effective so that the DP ship can maintain the desired position, heading and velocities in the existence of varying environment disturbances.展开更多
Given the uncertainty of parameters and the random nature of disturbances that effect a ships course, a robust course controller should be designed on the basis of rudder/flap vector control. This paper analyzes syste...Given the uncertainty of parameters and the random nature of disturbances that effect a ships course, a robust course controller should be designed on the basis of rudder/flap vector control. This paper analyzes system uncertainty, and the choice of weighting functions is also discussed. When sea waves operate on a ship, the energy-concentrating frequency varies with the angle of encounter. For different angles of encounter, different weighting functions are designed. For the pole of a nominal model existing in an imaginary axis, the bilinear-transform method is used. The "2-Riccati" equation is adopted to solve the H∞ controller. A system simulation is given, and the results show that, compared with a PID controller, this system has higher course precision and more robust performance. This research has significant engineering value.展开更多
Ship rolling in random waves is a complicated nonlinear motion that contributes substantially to ship instability and capsizing.The finite element method(FEM)is employed in this paper to solve the Fokker Planck(FP)equ...Ship rolling in random waves is a complicated nonlinear motion that contributes substantially to ship instability and capsizing.The finite element method(FEM)is employed in this paper to solve the Fokker Planck(FP)equations numerically for homoclinic and heteroclinic ship rolling under random waves described as periodic and Gaussian white noise excitations.The transient joint probability density functions(PDFs)and marginal PDFs of the rolling responses are also obtained.The effects of stimulation strength on ship rolling are further investigated from a probabilistic standpoint.The homoclinic ship rolling has two rolling states,the connection between the two peaks of the PDF is observed when the periodic excitation amplitude or the noise intensity is large,and the PDF is remarkably distributed in phase space.These phenomena increase the possibility of a random jump in ship motion states and the uncertainty of ship rolling,and the ship may lose stability due to unforeseeable facts or conditions.Meanwhile,only one rolling state is observed when the ship is in heteroclinic rolling.As the periodic excitation amplitude grows,the PDF concentration increases and drifts away from the beginning location,suggesting that the ship rolling substantially changes in a cycle and its stability is low.The PDF becomes increasingly uniform and covers a large region as the noise intensity increases,reducing the certainty of ship rolling and navigation safety.The current numerical solutions and analyses may be applied to evaluate the stability of a rolling ship in irregular waves and capsize mechanisms.展开更多
The mathematical model of optimal placement of active members in truss adaptive structures is essentially a nonlinear multi-objective optimization problem with mixed variables. It is usually much difficult and costly ...The mathematical model of optimal placement of active members in truss adaptive structures is essentially a nonlinear multi-objective optimization problem with mixed variables. It is usually much difficult and costly to be solved. In this paper, the optimal location of active members is treated in terms of （0, 1） discrete variables. Structural member sizes, control gains, and （0, 1） placement variables are treated simultaneously as design variables. Then, a succinct and reasonable compromise scalar model, which is transformed from original multi-objective optimization, is established, in which the （0, 1） discrete variables are converted into an equality constraint. Secondly, by penalty function approach, the subsequent scalar mixed variable compromise model can be formulated equivalently as a sequence of continuous variable problems. Thirdly, for each continuous problem in the sequence, by choosing intermediate design variables and temporary critical constraints, the approximation concept is carried out to generate a sequence of explicit approximate problems which enhance the quality of the approximate design problems. Considering the proposed method, a FORTRAN program OPAMTAS2.0 for optimal placement of active members in truss adaptive structures is developed, which is used by the constrained variable metric method with the watchdog technique （CVMW method）. Finally, a typical 18 bar truss adaptive structure as test numerical examples is presented to illustrate that the design methodology set forth is simple, feasible, efficient and stable. The established scalar mixed variable compromise model that can avoid the ill-conditioned possibility caused by the different orders of magnitude of various objective functions in optimization process, therefore, it enables the optimization algorithm to have a good stability. On the other hand, the proposed novel optimization technique can make both discrete and continuous variables be optimized simultaneously.展开更多
基金Supported by the National Natural Science Foundation of China,No.81970454.
文摘BACKGROUND ATP-binding cassette subfamily B member 4(ABCB4)deficiency is associated with cholestatic liver disease primarily because of missense mutations,and many variants remain unidentified.Here,we validate the pathogenicity and mechanism of ABCB4 variants in clinical and in vitro trials,hypothesizing that these variants are responsible for impaired biliary function and contribute to the development of cholestatic liver diseases.AIM To clarify the functional features and pathogenicity of ABCB4 variants.METHODS Clinical data were collected from five patients with cholestatic liver disease that was initially not detected by routine examinations.Later,whole-exome sequencing confirmed ABCB4 variants and the patients were treated from January 2017 to December 2023.Pathogenic mechanisms were analyzed using bioinformatics tools,and a cell model in vitro was established to investigate ABCB4 mRNA expression,multidrug resistance protein 3(MDR3)expression,cellular localization,and phosphatidylcholine secretion.Results were compared using Student's t-tests.RESULTS Five missense variants(c.1757T>A,c.1865G>A,c.2362C>T,c.2777C>T and c.3250C>T),one intron variant(c.537-32G>T),and one synonymous(c.C504T)variant were identified.Three of the five patients had various degrees of cholestasis,two presented with liver cirrhosis,and all had elevated gamma-glutamyl transferase.Three of the four patients who underwent a liver biopsy had bile duct dilation,and one had gallstones.Two of the four patients had normal and reduced MDR3 immunohistochemical levels.Bioinformatic analysis indicated that these variants were likely pathogenic except c.C504T variant.None of the missense variants influenced subcellular MDR3 Localization in vitro.However,the c.1865G>A variant significantly decreased ABCB4 mRNA values,and all missense variants down-regulated phosphatidylcholine secretion.CONCLUSION This study uncovered new ABCB4 variants and emphasized the pathogenic potential of specific variants.The findings from five patients provided insight into the pathogenic mechanisms underlying ABCB4-related diseases.
基金supported by Elam M. and Georgina E.Hack Memorial Research Funds,Department of Periodontics,University of Washington,Seattle,WA,USAsupported by WVCTSI funds,West Virginia University,Morgantown,WV,USA
文摘Toll-like receptor （TLR）-mediated inflammatory response could negatively affect bone metabolism. In this study, we determined how osteogenesis is regulated during inflammatory responses that are downstream of TLR signaling. Human primary osteoblasts were cultured in collagen gels. Pam3CSK4 （P3C） and Escherichia coli lipopolysaccharide （EcLPS） were used as TLR2 and TLR4 ligand respectively. Porphyromonas gingivalis LPS having TLR2 activity with either TLR4 agonism （Pg1690） or TLR4 antagonism （Pg1449） and mutant E. coli LPS （LPxE/LPxF/WSK） were used. IL-lp, SH2-containing inositol phosphatase-1 （SHIP1） that has regulatory roles in osteogenesis, alkaline phosphatase and mineralization were analyzed. 3α-Aminocholestane （3AC） was used to inhibit SHIP1. Our results suggest that osteoblasts stimulated by P3C, poorly induced IL-1β but strongly upregulated SHIP1 and enhanced osteogenic mediators. On the contrary, EcLPS significantly induced IL-1β and osteogenic mediators were not induced. While Pg1690 downmodulated osteogenic mediators, Pg1449 enhanced osteogenic responses, suggesting that TLR4 signaling annuls osteogenesis even with TLR2 activity. Interestingly, mutant E. coli LPS that induces weak inflammation upregulated osteogenesis, but SHIP1 was not induced. Moreover, inhibiting SHIP1 significantly upregulated TLR2-mediated inflammatory response and downmodulated osteogenesis. In conclusion, these results suggest that induction of weak inflammatory response through TLR2 （with SHIP1 activity） and mutant TLR4 ligands could enhance osteogenesis.
基金supported by the Director Foundation of Institute of Seismology,China Earthquake Administration(IS201326125)the National Natural Science Foundation of China(41204019,41304003)
文摘In this paper, the admittance function between seafloor undulations and vertical gravity gradient anomalies was derived. Based on this admittance function, the bathymetry model of 1 minute resolution was predicted from vertical gravity gradient anomalies and ship soundings in the experimental area from the northwest Pacific. The accuracy of the model is evaluated using ship soundings and existing models, including ETOPO1, GEBCO, DTU10 and V15.1 from SIO. The model＇s STD is 69. 481m, comparable with V15.1 which is generally believed to have the highest accuracy.
基金supported by the National Natural Science Foundation of China (31371618)the Research Plan of Application Base of Hebei, China (17962901D)
文摘Through regulating target genes via the mechanisms of posttranscriptional cleavage or translational repression, plant miRNAs involve diverse biological processes associating with plant growth, development, and abiotic stress responses, in this study, we functionally characterized TaMIR1119, a miRNA family member of wheat （Triticum aestivum）, in regulating the drought adaptive response of plants. TaMIR1119 putatively targets six genes categorized into the functional classes of transcriptional regulation, RNA and biochemical metabolism, trafficking, and oxidative stress defense. Upon simulated drought stress, the TaMIR1119 transcripts abundance in roots was drastically altered, showing to be upregulated gradually within a 48-h drought regime andthat the drought-induced transcripts were gradually restored along with a 48-h recovery treatment. In contrast, most miRNA target genes displayed reverse expression patterns to TaMIR1119, exhibiting a downregulated expression pattern upon drought and whose reduced transcripts were re-elevated along with a normal recovery treatment. These expression analysis results indicated that TaMIR1119 responds to drought and regulates the target genes mainly through a cleavage mechanism. Under drought stress, the tobacco lines with TaMIR1119 overexpression behaved improved phenotypes,, showing increased plant biomass, photosynthetic parameters, osmolyte accumulation, and enhanced antioxidant enzyme （AE） activities relative to wild type. Three AE genes, NtFeSOD, NtCAT1;3, and NtSOD2,1, encoding superoxide dismutase （SOD）, catalase （CAT）, and peroxidase （POD） proteins, respectively, showed upregulated expression in TaMIR1119 overexpression lines, suggesting that they are involved in the regulation of AE activities and contribution to the improved cellular reactive oxygen species （ROS） homeostasis in drought-challenged transgenic lines. Our results indicate that TaMIR1119 plays critical roles in regulating plant drought tolerance through transcriptionally regulating the target genes that modulate osmolyte accumulation, photosynthetic function, and improve cellular ROS homeostasis of plants.
基金Supported by the German Federal Ministry for Education and Research, No. 01ZZ96030, from the Ministry for Education, Research and Cultural Affairs and the Ministry for Social Affairs of the State Mecklenburg-West Pomerania
文摘AIM： To investigate those associations using data of the population-based Study of Health in Pomerania. METHODS： A study population of 3 749 residents aged 20-79 years without previously diagnosed thyroid disease was available for analyses. Serum TSH was used to assess thyroid function. Cholelithiasis was defined by either a prior history of cholecystectomy or the presence of gallstones on ultrasound. Logistic regression was performed to analyze independent associations between thyroid function and cholelithiasis. RESULTS： There were 385 persons （10.3%） with low （〈0.3 mIU/L）, 3 321 persons （88.6%） with normal and 43 persons （1.2%） with high serum TSH levels （〉3 mIU/L）. The proportion of cholelithiasis among males and females was 14.4% and 25.3%, respectively. Among males, there was an independent relation between high serum TSH and cholelithiasis （OR 3.77; 95%-CI 1.06-13.41; P〈0.05）. Also among males, there was a tendency towards an elevated risk of cholelithiasis in persons with low serum TSH （OR 1.40; 95%-CI 0.96-2.02; P= 0.07）. In the female population, no such relation was identified. CONCLUSION： There is an association between thyroid and gallstone disease with a gender-specific relation between hypothyroidism and cholelithiasis.
基金financially supported by the National Basic Research Program of China(973 Program,Grant No.2014CB046203)
文摘A domain decomposition and matching method in the time-domain is outlined for simulating the motions of ships advancing in waves. The flow field is decomposed into inner and outer domains by an imaginary control surface, and the Rankine source method is applied to the inner domain while the transient Green function method is used in the outer domain. Two initial boundary value problems are matched on the control surface. The corresponding numerical codes are developed, and the added masses, wave exciting forces and ship motions advancing in head sea for Series 60 ship and S175 containership, are presented and verified. A good agreement has been obtained when the numerical results are compared with the experimental data and other references. It shows that the present method is more efficient because of the panel discretization only in the inner domain during the numerical calculation, and good numerical stability is proved to avoid divergence problem regarding ships with flare.
基金financially supported by the Project of"Nonlinear Wave Excitation and Response of Surface Vehicle"(Grant No.B2420132001)the Natural Science Foundation of Tianjin(Grant No.15JCQNJC07700)
文摘The PDFs(probability density functions) and probability of a ship rolling under the random parametric and forced excitations were studied by a semi-analytical method. The rolling motion equation of the ship in random oblique waves was established. The righting arm obtained by the numerical simulation was approximately fitted by an analytical function. The irregular waves were decomposed into two Gauss stationary random processes, and the CARMA(2, 1) model was used to fit the spectral density function of parametric and forced excitations. The stochastic energy envelope averaging method was used to solve the PDFs and the probability. The validity of the semi-analytical method was verified by the Monte Carlo method. The C11 ship was taken as an example, and the influences of the system parameters on the PDFs and probability were analyzed. The results show that the probability of ship rolling is affected by the characteristic wave height, wave length, and the heading angle. In order to provide proper advice for the ship’s manoeuvring, the parametric excitations should be considered appropriately when the ship navigates in the oblique seas.
文摘The effect of coupling between sloshing and ship motions in the evaluation of slosh-induced interior pressures is studied. The coupling between sloshing loads and ship motions is modelled through a hybrid algorithm which combines a potential flow solution based on transient Green function for the external ship hydrodynamics with a viscous flow solution based on a multiphase interface capturing volume of fluid(VOF) technique for the interior sloshing motion. The coupled algorithm accounts for full nonlinear slosh forces while the external forces on the hull are determined through a blended scheme of linear radiationdiffraction with nonlinear Froude-Krylov and restoring forces. Consideration of this level of nonlinearities in ship motions is found to have non-negligible effects on the slosh-coupled responses and slosh-induced loads. A scheme is devised to evaluate the statistical measure of the pressures through long-duration simulation studies in extreme irregular waves. It is found that coupling significantly influences the tank interior pressures, and the differences in the pressures between coupled and uncoupled cases can be as much as 100% or more. To determine the RAO over the frequency range needed for the simulation studies in irregular waves, two alternative schemes are proposed, both of which require far less computational time compared to the conventional method of finding RAO at each frequency, and the merits of these are discussed.
文摘Adopting complex number theory, a mathematic model of Green function is built for two dimension free water surface, and an analytic expression of Green function is obtained by introducing two parameters. The intrinsic properties of Green function are discussed on vertical line and horizontal line. At last, the derivation expression of Green function is obtained from the formula of Green function.
基金the National Natural Science Foundation of China(No.51579114)the Project of New Century Excellent Talents of Colleges and Universities of Fujian Province(No.JA12181)the Project of Young and Middle-Aged Teacher Education of Fujian Province(No.JAT170309)
文摘A robust H∞sampled-data stabilization problem for nonlinear dynamic positioning(DP) ships with Takagi-Sugeno(T-S) fuzzy models is discussed in this paper. Input delay approach is used to convert the sampleddata DP ship system to a fuzzy system with time-varying delay. Adequate conditions are derived to determine the system's asymptotical stability and achieve H∞performance via Lyapunov stability theorems. Then, the fuzzy sampled-data controller is obtained by analyzing the stabilization condition. Simulation result shows that the proposed method and the designed controller for a DP ship are effective so that the DP ship can maintain the desired position, heading and velocities in the existence of varying environment disturbances.
文摘Given the uncertainty of parameters and the random nature of disturbances that effect a ships course, a robust course controller should be designed on the basis of rudder/flap vector control. This paper analyzes system uncertainty, and the choice of weighting functions is also discussed. When sea waves operate on a ship, the energy-concentrating frequency varies with the angle of encounter. For different angles of encounter, different weighting functions are designed. For the pole of a nominal model existing in an imaginary axis, the bilinear-transform method is used. The "2-Riccati" equation is adopted to solve the H∞ controller. A system simulation is given, and the results show that, compared with a PID controller, this system has higher course precision and more robust performance. This research has significant engineering value.
基金the National Natural Science Foundation of China(Nos.52088102,51875540)。
文摘Ship rolling in random waves is a complicated nonlinear motion that contributes substantially to ship instability and capsizing.The finite element method(FEM)is employed in this paper to solve the Fokker Planck(FP)equations numerically for homoclinic and heteroclinic ship rolling under random waves described as periodic and Gaussian white noise excitations.The transient joint probability density functions(PDFs)and marginal PDFs of the rolling responses are also obtained.The effects of stimulation strength on ship rolling are further investigated from a probabilistic standpoint.The homoclinic ship rolling has two rolling states,the connection between the two peaks of the PDF is observed when the periodic excitation amplitude or the noise intensity is large,and the PDF is remarkably distributed in phase space.These phenomena increase the possibility of a random jump in ship motion states and the uncertainty of ship rolling,and the ship may lose stability due to unforeseeable facts or conditions.Meanwhile,only one rolling state is observed when the ship is in heteroclinic rolling.As the periodic excitation amplitude grows,the PDF concentration increases and drifts away from the beginning location,suggesting that the ship rolling substantially changes in a cycle and its stability is low.The PDF becomes increasingly uniform and covers a large region as the noise intensity increases,reducing the certainty of ship rolling and navigation safety.The current numerical solutions and analyses may be applied to evaluate the stability of a rolling ship in irregular waves and capsize mechanisms.
基金supported by National Natural Science Foundation of China(Grant No.10472007)
文摘The mathematical model of optimal placement of active members in truss adaptive structures is essentially a nonlinear multi-objective optimization problem with mixed variables. It is usually much difficult and costly to be solved. In this paper, the optimal location of active members is treated in terms of （0, 1） discrete variables. Structural member sizes, control gains, and （0, 1） placement variables are treated simultaneously as design variables. Then, a succinct and reasonable compromise scalar model, which is transformed from original multi-objective optimization, is established, in which the （0, 1） discrete variables are converted into an equality constraint. Secondly, by penalty function approach, the subsequent scalar mixed variable compromise model can be formulated equivalently as a sequence of continuous variable problems. Thirdly, for each continuous problem in the sequence, by choosing intermediate design variables and temporary critical constraints, the approximation concept is carried out to generate a sequence of explicit approximate problems which enhance the quality of the approximate design problems. Considering the proposed method, a FORTRAN program OPAMTAS2.0 for optimal placement of active members in truss adaptive structures is developed, which is used by the constrained variable metric method with the watchdog technique （CVMW method）. Finally, a typical 18 bar truss adaptive structure as test numerical examples is presented to illustrate that the design methodology set forth is simple, feasible, efficient and stable. The established scalar mixed variable compromise model that can avoid the ill-conditioned possibility caused by the different orders of magnitude of various objective functions in optimization process, therefore, it enables the optimization algorithm to have a good stability. On the other hand, the proposed novel optimization technique can make both discrete and continuous variables be optimized simultaneously.
