Guided by the fundamental principles of the whole petroleum system,the control of tectonism,sedimentation,and diagenesis on hydrocarbon accumulation in a rifted basin is studied using the data of petroleum geology and...Guided by the fundamental principles of the whole petroleum system,the control of tectonism,sedimentation,and diagenesis on hydrocarbon accumulation in a rifted basin is studied using the data of petroleum geology and exploration of the second member of the Paleogene Kongdian Formation(Kong-2 Member)in the Cangdong Sag,Bohai Bay Basin,China.It is clarified that the circle structure and circle effects are the marked features of a continental fault petroliferous basin,and they govern the orderly distribution of conventional and unconventional hydrocarbons in the whole petroleum systems of the rifted basin.Tectonic circle zones control sedimentary circle zones,while sedimentary circle zones and diagenetic circle zones control the spatial distribution of favorable reservoirs,thereby determining the orderly distribution of hydrocarbon accumulations in various circles.A model for the integrated,systematic accumulation of conventional and unconventional hydrocarbons under a multi-circle structure of the whole petroleum system of continental rifted basin has been developed.It reveals that each sag of the rifted basin is an independent whole petroleum system and circle system,which encompasses multiple orderly circles of conventional and unconventional hydrocarbons controlled by the same source kitchen.From the outer circle to the middle circle and then to the inner circle,there is an orderly transition from structural and stratigraphic reservoirs,to lithological and structural-lithological reservoirs,and finally to tight oil/gas and shale oil/gas enrichment zones.The significant feature of the whole petroleum system is the orderly control of hydrocarbons by multi-circle stratigraphic coupling,with the integrated,orderly distribution of conventional and unconventional reserves being the inevitable result of the multi-layered interaction within the whole petroleum system.This concept of multi-circle stratigraphic coupling for the orderly,integrated accumulation of conventional and unconventional hydrocarbons has guided significant breakthroughs in the overall,three-dimensional exploration and shale oil exploration in the Cangdong Sag.展开更多
Adaptive truss structures are a new kind of structures with integrated active members,whose dynamic characteristies can be beneficially modified to meet mission requirements.Active members containing actuating and sen...Adaptive truss structures are a new kind of structures with integrated active members,whose dynamic characteristies can be beneficially modified to meet mission requirements.Active members containing actuating and sensing units are the major components of adaptive truss structures.Modeling of adaptive truss structures is a key step to analyze the structural dynamic characteristics.A new experimental modal analysis approach,in which active members are used as excitatiDn sources for modal test,has been proposed in this paper.The excitation forces generated by the active members, which are different from the excitation forces exerted on structures in the conventional modal test,are internal forces for the truss structures.The relation between internal excitation forces and external forces is revealed such that the traditional identification method can be adopted to obtain modal parameters of adaptive structures.Placement problem of the active member in adaptive truss structures is also discussed in this work. Modal test and analysis are conducted with a planar adaptive truss structure by using piezoelectric active members in order to verify the feasibility and effectiveness of the proposed method.展开更多
The gravity flow deposit were mainly developed in the lowstand systems tract（LST） of the first member of Upper Miocene Huangliu Formation（Ehl1） in Dongfang area, Yinggehai Basin, has become a valuable target for g...The gravity flow deposit were mainly developed in the lowstand systems tract（LST） of the first member of Upper Miocene Huangliu Formation（Ehl1） in Dongfang area, Yinggehai Basin, has become a valuable target for gas exploration and production. The gravity flow sedimentary characteristics of lithofacies associations, sedimentary texture, seismic facies and logging facies were described in detail on the basis of integrated analysis of cores, logging and seismic data. The sedimentary microfacies types composed of neritic sandbar, continental shelf mud, main channel, bifurcated or cross-cutting distributary channel, overspill, and natural levee are revealed under the constraint of high resolution sequence stratigraphic framework in the Ehl1. The gravity flow deposit system in the LST is divided into three evolution stages corresponding to periods of three parasequence sets. The gravity flow deposit was induced in the early LST, expanded rapidly in the middle LST and decreased slightly in the late LST. But its developing scale decreased sharply in the transgression systems tract（TST） and finally vanished in the highstand systems tract（HST）. This spatial evolution rule is constrained by the integrated function of sediments supply of the Vietnam Blue River in the LST, the development of local gradient change in sea floor（micro-topography, i.e., flexure slope break）, and the fall in relative sea level. On the basics of the deep study of the coupling relationship among the three main control factors, the sedimentary model is established as an optimal component of ＂source-channel-sink＂ for shallow marine turbidite submarine fan.展开更多
1 Introduction Shale formations bear abundant mineral resource and*unconventional petroleum resource,and the unconventional petroleum resource that contain in the shale formation should be integrated and researched.
According to the legal definitions of land transfer under shareholding cooperative systems,rural groups,collective rights of members,and this paper discloses the necessity of protecting the collective rights of member...According to the legal definitions of land transfer under shareholding cooperative systems,rural groups,collective rights of members,and this paper discloses the necessity of protecting the collective rights of members.In terms of qualification definition of members,rights of fetus,married daughters and sons in law cannot be guaranteed.In terms of rights protection,partial rights cannot be assured,power of personal stake inthe collective members is imperfect which results in inadequate,unbalanced,shaky and unpunctuated benefits.Based on objective and subjectivecauses,the democratic management rights of members in land transfer process have been weakened or even deprived,the protection of the basiclife is hard to realize.The paper proposes that it is necessary to build the social protection mechanisms of rural land shareholding cooperative sys-tems.The first is to treat those members that own land,attending the shareholding cooperation but having not taken part in the land transfer proces-sion as the protection objects.The second is in the procession of land transfer,making it clear the compensation standards of land transfer,estab-lishing the intermediate agencies of land transfer and performing the collective economic organizing functions.The third is that the account capital ofsocial protection is contributed together by personal member,the land users(shareholding cooperative companies) and government.The fourth isto pay more attention to the added value and safety management of capital operation.展开更多
I the context of the Corporate Governance Code enactment in Japan, we examine how newly introduced outside directors in Japanese boards obtain information to take part in the decision-making process. We conducted a sy...I the context of the Corporate Governance Code enactment in Japan, we examine how newly introduced outside directors in Japanese boards obtain information to take part in the decision-making process. We conducted a systematic review of the literature and found 18 peer-reviewed publications in a time span between 2000 and 2016 that described the asymmetry of information between the insider group of board directors （including the CEO） and the outside board members. Our fmdings show that for the course of more than a decade, despite all changes and reforms, the role of board directors, whether insiders or outsiders, is still supplementary. They are treated more as advisors than active part in the decision-making process. We reveal different insider sources of information as forming social ties with the CEO and/or inside board directors and collaboration with Audit ＆ Supervisory Board （Kansayaku）, which can help reduce this asymmetry and improve the decision-making process. We assume that it will be easier for the outsiders to establish contacts and form social ties with the Audit ＆ Supervisory Board members because of their unspoken lower status and thus to obtain more information about the company internal affairs and discussions that take place during the informal meetings, where only insiders （including the CEO） are present.展开更多
BACKGROUND Colorectal cancer(CRC)is the third most common cancer globally,causing over 900000 deaths annually.Risk factors include aging,diet,obesity,sedentary lifestyle,tobacco use,genetic predisposition,and inflamma...BACKGROUND Colorectal cancer(CRC)is the third most common cancer globally,causing over 900000 deaths annually.Risk factors include aging,diet,obesity,sedentary lifestyle,tobacco use,genetic predisposition,and inflammatory bowel disease.Despite current treatments,survival rates for advanced CRC remain low,highlighting the need for better therapeutic strategies.AIM To evaluate both the clinical significance and the pathological implications of the Kinesin family member 14(KIF14)expression within CRC specimens.Additionally,this study aims to investigate the interaction between nitidine chloride(NC)and KIF14,considering their potential as therapeutic targets.METHODS The expression of the KIF14 protein in CRC was analyzed using immunohistochemical staining.The integration of multicenter high-throughput data facilitated the calculation of the standardized mean difference(SMD)for KIF14 mRNA levels.The assessment of clinical and pathological impact was enhanced by analyzing combined receiver operating characteristic curves,along with measures of sensitivity,specificity,and likelihood ratios.Additionally,clustered regularly interspaced short palindromic repeats knockout screening for cell growth and single-cell sequencing were employed to validate the significance of KIF14 expression in CRC.Survival analysis established the prognostic value of KIF14 in CRC.The molecular mechanism of NC against CRC was elucidated through whole-genome sequencing and enrichment analysis,and molecular docking was utilized to explore the targeting affinity between NC and KIF14.RESULTS KIF14 was highly expressed in 208 CRC patients.Data from 17 platforms involving 2436 CRC samples and 1320 noncancerous colorectal tissue controls indicated that KIF14 expression was significantly higher in CRC samples,with an SMD of 1.92(95%CI:1.49-2.35).The area under the curve was 0.94(95%CI:0.92-0.96),with a sensitivity of 0.85(95%CI:0.78-0.90)and a specificity of 0.90(95%CI:0.85-0.93).The positive and negative likelihood ratios were 8.38(95%CI:5.39-13.02)and 0.17(95%CI:0.11-0.26),respectively.At the single-cell level,significant overexpression of KIF14 was observed in CRC cells(P<0.001),with 35 CRC cell lines dependent on KIF14 for growth.The K-M plots demonstrated that KIF14 possesses prognostic value in CRC patients within the GSE71187 and GSE103679 datasets(P<0.05).Binding energy calculations indicated that KIF14 is a potential target for NC(binding energy:10.3 kcal/mol).CONCLUSION KIF14 promotes the growth of CRC cells and acts as an oncogenic factor,potentially serving as a therapeutic target for NC in the treatment of CRC.展开更多
In accordance with the organizational development process and management of party members, in this article it is based on the C/S and B/S mixed structure to finish the application design of client and servers, and usi...In accordance with the organizational development process and management of party members, in this article it is based on the C/S and B/S mixed structure to finish the application design of client and servers, and using the language of VB.NET develop with Inner Mongolia Agricultural University, members of University College Digital Management Information System. The system from research projects of Agriculture University Occupation Technical College of Inner Mongolia.展开更多
Dongyuemiao Member shale in the Sichuan Basin,China,is characterized by organic-rich shale intervals with different types of interbeds and accumulation modes.The aim of this study is to elucidate the impact of paleoen...Dongyuemiao Member shale in the Sichuan Basin,China,is characterized by organic-rich shale intervals with different types of interbeds and accumulation modes.The aim of this study is to elucidate the impact of paleoenvironmental indicators on interbed development.With this aim in mind,we established an interbed classification scheme and quantified the development of different types of interbeds and their frequencies.We categorized the shale interbeds into three types based on interbed type:silt interbeds(SIs),shell fragment interbeds(SFIs),and shell skeleton interbeds(SSIs).The SIs,SFIs,and SSIs are respectively the products of extrabasinal low-density turbidity currents,intrabasinal debris flow,and intrabasinal low-density turbidity currents.We propose that variations in paleoenvironmental conditions primarily influenced the types of interbeds that developed but had minimal impact on the frequency of their development.Models depicting the interbed development within the 1st Submember of Dongyuemiao Member indicate that during the early Dongyuemiao depositional period,under conditions of relatively aridity,weak weathering,high terrigenous input,and strong hydrodynamic activity,SSIs were well developed.In the middle depositional period,as the climate gradually transitioned to more humid conditions,and the weathering intensity and amount of terrestrial input increased,the development of SIs and SFIs significantly increased.During the late depositional period,with a continuous decrease in terrestrial inputs and sedimentation rates,the development of SIs decreased while that of SSIs increased.展开更多
In this editorial,we comment on the article by Qin et al,recently published in the World Journal of Gastrointestinal Oncology.Malignant tumors of the digestive tract represent a significant health threat.Kinesin famil...In this editorial,we comment on the article by Qin et al,recently published in the World Journal of Gastrointestinal Oncology.Malignant tumors of the digestive tract represent a significant health threat.Kinesin family member 14(KIF14),a critical kinesin,is pivotal in the proliferation,migration,and invasion of tumor cells.It has emerged as a focal point in recent studies of malignant tumors in the digestive tract.This article reviews the current research on KIF14 within these tumors and details its significant role in tumor cell behaviors,including proliferation,apo-ptosis,migration,invasion,and angiogenesis,alongside the regulatory mechanisms of the associated intracellular signaling pathways.Additionally,it explores the clinical value of KIF14 as a potential biomarker for early diagnosis,disease monitoring,and prognostic evaluation in malignant tumors of the digestive tract.The article concludes by introducing the potential regulatory role of traditional Chinese medicine,aiming to combine the strengths of both modern and traditional medical approaches to enhance treatment outcomes and prognosis for patients with these tumors.展开更多
Taking the second member of the Xujiahe Formation of the Upper Triassic in the Xinchang structural belt as an example,based on data such as logging,production,seismic interpretation and test,a systematic analysis was ...Taking the second member of the Xujiahe Formation of the Upper Triassic in the Xinchang structural belt as an example,based on data such as logging,production,seismic interpretation and test,a systematic analysis was conducted on the structural characteristics and evolution,reservoir diagenesis and densification processes,and types and stages of faults/fractures,and revealing the multi-stage and multi-factor dynamic coupled enrichment mechanisms of tight gas reservoirs.(1)In the early Yanshan period,the paleo-structural traps were formed with low-medium maturity hydrocarbons accumulating in structural highs driven by buoyancy since reservoirs were not fully densified in this stage,demonstrating paleo-structure control on traps and early hydrocarbon accumulation.(2)In the middle-late Yanshan period,the source rocks became mature to generate and expel a large quantity of hydrocarbons.Grain size and type of sandstone controlled the time of reservoir densification,which restricted the scale of hydrocarbon charging,allowing for only a small-scale migration through sand bodies near the fault/fracture or less-densified matrix reservoirs.(3)During the Himalayan period,the source rocks reached overmaturity,and the residual oil cracking gas was efficiently transported along the late-stage faults/fractures.Wells with high production capacity were mainly located in Type I and II fault/fracture zones comprising the late-stage north-south trending fourth-order faults and the late-stage fractures.The productivity of the wells was controlled by the transformation of the late-stage faults/fractures.(4)The Xinchang structural belt underwent three stages of tectonic evolution,two stages of reservoir formation,and three stages of fault/fractures development.Hydrocarbons mainly accumulated in the paleo-structure highs.After reservoir densification and late fault/fracture adjustment,a complex gas-water distribution pattern was formed.Thus,it is summarized as the model of“near-source and low-abundance hydrocarbon charging in the early stage,and differential enrichment of natural gas under the joint control of fault-fold-fracture complex,high-quality reservoirs and structural highs in the late stage”.Faults/fractures with well-coupled fault-fold-fracture-pore are favorable exploration targets with high exploration effectiveness.展开更多
At 4:50 on April 30, China's LM-3B/I rocket, an improved type based on LM-3B, made its debut at the Xichang Satellite Launch Center and successfully sending the 12th and 13th BeiDou Navigation Satellite System sat...At 4:50 on April 30, China's LM-3B/I rocket, an improved type based on LM-3B, made its debut at the Xichang Satellite Launch Center and successfully sending the 12th and 13th BeiDou Navigation Satellite System satellites into the planned transfer orbit in space. It was the first time that China launched two BeiDou satellites with one rocket. It was展开更多
Skeletal muscles are essential for locomotion,posture,and metabolic regulation.To understand physiological processes,exercise adaptation,and muscle-related disorders,it is critical to understand the molecular pathways...Skeletal muscles are essential for locomotion,posture,and metabolic regulation.To understand physiological processes,exercise adaptation,and muscle-related disorders,it is critical to understand the molecular pathways that underlie skeletal muscle function.The process of muscle contra ction,orchestrated by a complex interplay of molecular events,is at the core of skeletal muscle function.Muscle contraction is initiated by an action potential and neuromuscular transmission requiring a neuromuscular junction.Within muscle fibers,calcium ions play a critical role in mediating the interaction between actin and myosin filaments that generate force.Regulation of calcium release from the sarcoplasmic reticulum plays a key role in excitation-contraction coupling.The development and growth of skeletal muscle are regulated by a network of molecular pathways collectively known as myogenesis.Myogenic regulators coordinate the diffe rentiation of myoblasts into mature muscle fibers.Signaling pathways regulate muscle protein synthesis and hypertrophy in response to mechanical stimuli and nutrient availability.Seve ral muscle-related diseases,including congenital myasthenic disorders,sarcopenia,muscular dystrophies,and metabolic myopathies,are underpinned by dys regulated molecular pathways in skeletal muscle.Therapeutic interventions aimed at preserving muscle mass and function,enhancing regeneration,and improving metabolic health hold promise by targeting specific molecular pathways.Other molecular signaling pathways in skeletal muscle include the canonical Wnt signaling pathway,a critical regulator of myogenesis,muscle regeneration,and metabolic function,and the Hippo signaling pathway.In recent years,more details have been uncovered about the role of these two pathways during myogenesis and in developing and adult skeletal muscle fibers,and at the neuromuscular junction.In fact,research in the last few years now suggests that these two signaling pathways are interconnected and that they jointly control physiological and pathophysiological processes in muscle fibers.In this review,we will summarize and discuss the data on these two pathways,focusing on their concerted action next to their contribution to skeletal muscle biology.However,an in-depth discussion of the noncanonical Wnt pathway,the fibro/a dipogenic precursors,or the mechanosensory aspects of these pathways is not the focus of this review.展开更多
Fibre and fabric production technologies-especially in the area of com-posite reinforcements-have played an as-yet largely unheralded role in thedevelopment of the UKs Formula One industry,but the British Textile Mach...Fibre and fabric production technologies-especially in the area of com-posite reinforcements-have played an as-yet largely unheralded role in thedevelopment of the UKs Formula One industry,but the British Textile MachineryAssociation(BTMA)aims to change that.展开更多
Published:25 September 2025 In the article“MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2”(BIOCELL,2021,Vol.45,No.3,pp.627-638.doi:10.32604/biocell.2021.01349...Published:25 September 2025 In the article“MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2”(BIOCELL,2021,Vol.45,No.3,pp.627-638.doi:10.32604/biocell.2021.013496),there is an error in the Fig.3.In order to ensure the scientific and rigorous nature of our academic publications,we made a correction about Fig.3 correspondingly.展开更多
BACKGROUND Grief counseling has become relatively established and is widely used among the families of cancer patients,effectively alleviating their psychological pain.However,in China,due to the influence of Confucia...BACKGROUND Grief counseling has become relatively established and is widely used among the families of cancer patients,effectively alleviating their psychological pain.However,in China,due to the influence of Confucianism and other traditional cultures,people generally adhere to the belief of“reincarnation to avoid death”,focusing more on themes of life,such as eugenics and longevity,and paying less attention to matters related to death,including death education and grief counseling.Currently,grief counseling in China is still in an exploratory stage,and there is relatively little research on the psychological status of family members of patients with terminal tumors.AIM To investigate the psychological effects of grief counseling on family members of terminal cancer patients.METHODS This study was designed as a randomized controlled trial that utilized convenience sampling to select family members of terminal tumor patients who were admitted to the hospice ward of the Affiliated Hospital of Jiangnan University from January to June 2025 as research subjects.All participants received conventional symptomatic supportive treatment and palliative care.Additionally,the intervention group benefited from extra grief counseling.RESULTS The Distress Thermometer(DT)score of the control group slightly decreased compared to before the intervention,but the difference was not statistically significant(P>0.05).In contrast,the DT score of the intervention group decreased significantly compared to before the intervention,with a statistically significant difference(P<0.05).After the intervention,the DT score of the intervention group was lower than that of the control group,and the difference was also statistically significant(P<0.05).After the intervention,the intervention group performed better DT level than the control group,with a statistically significant difference(P<0.05),the intervention group outperformed the control group in terms of depression and anxiety,and the difference was statistically significant(P<0.05).CONCLUSION Grief counseling can help alleviate the psychological pain and negative emotions experienced by family members of patients with terminal malignant tumors.展开更多
Subseasonal forecasting of extreme events is crucial for early warning systems.However,the forecast skills for extreme events are limited.Taking the extreme cold events in January 2018 as a specific example,and analyz...Subseasonal forecasting of extreme events is crucial for early warning systems.However,the forecast skills for extreme events are limited.Taking the extreme cold events in January 2018 as a specific example,and analyzing the 34 extreme cold events in East Asia from 1998 to 2020,the authors evaluated the forecast skills of the ECMWF model ensemble members on subseasonal time scales.The results show that while the ensemble mean has limited skills for forecasting extreme cold events at the 3-week lead time,some individual members demonstrate high forecast skills.For most extreme cold events,there are>10%of members among the total ensembles that can well predict the rapid temperature transitions at the 14-day lead time.This highlights the untapped potential of the ECMWF model to forecast extreme cold events on subseasonal time scales.High-skill ensemble members rely on accurate predictions of atmospheric circulation patterns(500-hPa geopotential height,mean sea level pressure)and key weather systems,including the Ural Blocking and Siberian High,that influence extreme cold events.展开更多
The lower member of Dalazi Formation is an important oil reservoir in the Songjiang Basin.Based on the research on its field-measured geological profiles,lithological combination characteristics and grain size distrib...The lower member of Dalazi Formation is an important oil reservoir in the Songjiang Basin.Based on the research on its field-measured geological profiles,lithological combination characteristics and grain size distribution characteristics,combined with the analysis of the spatial distribution characteristics,sedimentary structural characteristics and hydrodynamic conditions of the sediments in this member,nine sedimentary microfacies of the fan delta plain subfacies,fan delta front subfacies and littoral-shallow lacustrine subfacies have been identified.The study reveals that lower member of Dalazi Formation in the research area follows a fan delta–shallow lacustrine depositional model in the steep slope zone of a rift lake basin.The sediments primarily originate from the Pre-Mesozoic strata in the steep southeastern and eastern margins.The basin center migrated from the early Xiaoshahe area to the Yangmucun–Shenglicun area,and the sedimentary system gradually transitioned from fan delta to littoral-shallow lacustrine facies.展开更多
Understanding diagenetic processes plays a crucial role in evaluating the quality of tight reservoirs.In this study,we aimed to examine diagenetic facies and thereby assess reservoir quality by conducting an integrate...Understanding diagenetic processes plays a crucial role in evaluating the quality of tight reservoirs.In this study,we aimed to examine diagenetic facies and thereby assess reservoir quality by conducting an integrated analysis of porosity,petrology,permeability,mineralogy,mercury injection,and stable isotopic data in core samples from the Permian Shan 2 and He 1 members(Shan 2—He 1 Mbr)in China's Ordos Basin.Early compaction and precipitation of diagenetic minerals have significantly reduced primary pore space in these members,although certain sandstones have retained anomalously high porosity.These high-porosity reservoirs have been shaped by many factors,with dissolution identified as the predominant mechanism.Detailed petrographic observations and assessments of the primary minerals,the minerals'genesis,and associated diagenetic processes revealed seven distinct diagenetic facies differentiated by their sand-grain populations,types of cementation,clay matrix contents,and volcanic tuffaceous sediment(VTS).The main interstitial fillings consist of VTS,clay minerals,quartz overgrowths,and calcite(ferrocalcite).Clasts and VTS-dissolved sandstone have mainly formed Class I reservoirs with highly unstable grains and good secondary pores resulting from the dissolution of the grains and VTS.VTS tight sandstone,siliceous cementation tight sandstone,illite-siliceous cementation tight sandstone,and calcite cementation tight sandstone have formed Class II reservoirs with high rates of interstitial filling and cementation.Sandstone tightly cemented by illite has poor reservoir properties and has mainly formed Class III reservoirs.This classification underscores the critical importance of understanding diagenesis,diagenetic facies,and especially the diagenetic features of VTS for accurately assessing reservoir quality.A comprehensive understanding of these factors may facilitate more effective hydrocarbon exploration and exploitation in this area.展开更多
BACKGROUND Aberrant microRNAs expression and associated pathways have been proved participate in regulation vast various physiologic and pathologic processed of different human cancers including liver cancer.While,the...BACKGROUND Aberrant microRNAs expression and associated pathways have been proved participate in regulation vast various physiologic and pathologic processed of different human cancers including liver cancer.While,the function of miR-451a in liver cancer still indistinct.AIM To study the effect of miR-451a in liver cancer development.METHODS GeneChip microarray analysis performed to detect miR-451a expression in liver cancer tissues and normal liver tissues.Reverse transcription-polymerase chain reaction was used to validate the expression of miR-451a in liver cancer cell and other tumor cell lines.Construction of liver cancer cell lines that stably overexpressed miR-451a by transfecting Lentivirus produced by Genechem company.Methylthiazolyldiphenyl-tetrazolium(MTT)bromide assay and colony formation assay to determine the effect of miR-451a in liver cancer cell proliferation.Flow cytometry used to investigate whether miR-451a involved in liver cancer cell apoptosis.Cell migration ability was measured via wound scratch assay.Target gene was explored by bioinformatic analysis,and downstream molecule of miR-451a in liver cancer identified by rescue experiments.RESULTS MiR-451a expression significantly downregulation in liver cancer tissues compared with that in normal liver tissue.MiR-451a also obviously low-expressed in liver cancer cell,colorectal carcinoma cell and esophageal carcinoma cell lines.Human hepatoblastoma G2(HepG2)and BEL-7404 cell lines that stably overexpressed miR-451a by transfecting lentivirus constructed successfully.MTT bromide assay and colony formation assay showed that the overexpressed miR-451a inhibit HepG2 cell proliferation viability,but not BEL-7404 cell.Flow cytometry determined that miR-451a regulating proliferation not through inducing apoptosis.Wound scratch assay revealed that miR-451a overexpression suppressed HepG2 cell migration.Furthermore,mex-3 RNA binding family member C was predicted as the target gene by bioinformatic analysis,and rescue experiments confirmed the hypothesis.CONCLUSION Therefore,miR-451a may be candidate miRNA for understanding molecular mechanisms of liver cancer development and novel target in liver cancer cell.展开更多
基金Supported by the National Science and Technology Major Project of China(2024ZD1400101)China National Key Research and Development Project(2022YFF0801204)Major Science and Technology Project of CNPC(2023ZZ15YJ01,2021DJ0702)。
文摘Guided by the fundamental principles of the whole petroleum system,the control of tectonism,sedimentation,and diagenesis on hydrocarbon accumulation in a rifted basin is studied using the data of petroleum geology and exploration of the second member of the Paleogene Kongdian Formation(Kong-2 Member)in the Cangdong Sag,Bohai Bay Basin,China.It is clarified that the circle structure and circle effects are the marked features of a continental fault petroliferous basin,and they govern the orderly distribution of conventional and unconventional hydrocarbons in the whole petroleum systems of the rifted basin.Tectonic circle zones control sedimentary circle zones,while sedimentary circle zones and diagenetic circle zones control the spatial distribution of favorable reservoirs,thereby determining the orderly distribution of hydrocarbon accumulations in various circles.A model for the integrated,systematic accumulation of conventional and unconventional hydrocarbons under a multi-circle structure of the whole petroleum system of continental rifted basin has been developed.It reveals that each sag of the rifted basin is an independent whole petroleum system and circle system,which encompasses multiple orderly circles of conventional and unconventional hydrocarbons controlled by the same source kitchen.From the outer circle to the middle circle and then to the inner circle,there is an orderly transition from structural and stratigraphic reservoirs,to lithological and structural-lithological reservoirs,and finally to tight oil/gas and shale oil/gas enrichment zones.The significant feature of the whole petroleum system is the orderly control of hydrocarbons by multi-circle stratigraphic coupling,with the integrated,orderly distribution of conventional and unconventional reserves being the inevitable result of the multi-layered interaction within the whole petroleum system.This concept of multi-circle stratigraphic coupling for the orderly,integrated accumulation of conventional and unconventional hydrocarbons has guided significant breakthroughs in the overall,three-dimensional exploration and shale oil exploration in the Cangdong Sag.
文摘Adaptive truss structures are a new kind of structures with integrated active members,whose dynamic characteristies can be beneficially modified to meet mission requirements.Active members containing actuating and sensing units are the major components of adaptive truss structures.Modeling of adaptive truss structures is a key step to analyze the structural dynamic characteristics.A new experimental modal analysis approach,in which active members are used as excitatiDn sources for modal test,has been proposed in this paper.The excitation forces generated by the active members, which are different from the excitation forces exerted on structures in the conventional modal test,are internal forces for the truss structures.The relation between internal excitation forces and external forces is revealed such that the traditional identification method can be adopted to obtain modal parameters of adaptive structures.Placement problem of the active member in adaptive truss structures is also discussed in this work. Modal test and analysis are conducted with a planar adaptive truss structure by using piezoelectric active members in order to verify the feasibility and effectiveness of the proposed method.
基金supported by the National Natural Science Foundation of China (NSFC) (Nos. 41272122, 41202074, 41172123 and 40702023)the National Twelfth Five-Year Major Projects of Oil and Gas (No. 2011ZX05025-002-02-02)+1 种基金the Key Laboratory of Tectonics and Petroleum Resources (CUG)of Ministry of Education open issue (No. TPR-2013-08)the China University of Geosciences (Wuhan) Teaching Labor atory open funded projects
文摘The gravity flow deposit were mainly developed in the lowstand systems tract（LST） of the first member of Upper Miocene Huangliu Formation（Ehl1） in Dongfang area, Yinggehai Basin, has become a valuable target for gas exploration and production. The gravity flow sedimentary characteristics of lithofacies associations, sedimentary texture, seismic facies and logging facies were described in detail on the basis of integrated analysis of cores, logging and seismic data. The sedimentary microfacies types composed of neritic sandbar, continental shelf mud, main channel, bifurcated or cross-cutting distributary channel, overspill, and natural levee are revealed under the constraint of high resolution sequence stratigraphic framework in the Ehl1. The gravity flow deposit system in the LST is divided into three evolution stages corresponding to periods of three parasequence sets. The gravity flow deposit was induced in the early LST, expanded rapidly in the middle LST and decreased slightly in the late LST. But its developing scale decreased sharply in the transgression systems tract（TST） and finally vanished in the highstand systems tract（HST）. This spatial evolution rule is constrained by the integrated function of sediments supply of the Vietnam Blue River in the LST, the development of local gradient change in sea floor（micro-topography, i.e., flexure slope break）, and the fall in relative sea level. On the basics of the deep study of the coupling relationship among the three main control factors, the sedimentary model is established as an optimal component of ＂source-channel-sink＂ for shallow marine turbidite submarine fan.
基金supported by funding the National Basic Research Program of China (973 Program) and the grant number is 2014CB239000
文摘1 Introduction Shale formations bear abundant mineral resource and*unconventional petroleum resource,and the unconventional petroleum resource that contain in the shale formation should be integrated and researched.
基金Supported by the Collective Impact Survey of Rural Collective Members' Rights on the Land Transfer of National College Innovative Pilot Projects of 2009 (081035716)
文摘According to the legal definitions of land transfer under shareholding cooperative systems,rural groups,collective rights of members,and this paper discloses the necessity of protecting the collective rights of members.In terms of qualification definition of members,rights of fetus,married daughters and sons in law cannot be guaranteed.In terms of rights protection,partial rights cannot be assured,power of personal stake inthe collective members is imperfect which results in inadequate,unbalanced,shaky and unpunctuated benefits.Based on objective and subjectivecauses,the democratic management rights of members in land transfer process have been weakened or even deprived,the protection of the basiclife is hard to realize.The paper proposes that it is necessary to build the social protection mechanisms of rural land shareholding cooperative sys-tems.The first is to treat those members that own land,attending the shareholding cooperation but having not taken part in the land transfer proces-sion as the protection objects.The second is in the procession of land transfer,making it clear the compensation standards of land transfer,estab-lishing the intermediate agencies of land transfer and performing the collective economic organizing functions.The third is that the account capital ofsocial protection is contributed together by personal member,the land users(shareholding cooperative companies) and government.The fourth isto pay more attention to the added value and safety management of capital operation.
文摘I the context of the Corporate Governance Code enactment in Japan, we examine how newly introduced outside directors in Japanese boards obtain information to take part in the decision-making process. We conducted a systematic review of the literature and found 18 peer-reviewed publications in a time span between 2000 and 2016 that described the asymmetry of information between the insider group of board directors （including the CEO） and the outside board members. Our fmdings show that for the course of more than a decade, despite all changes and reforms, the role of board directors, whether insiders or outsiders, is still supplementary. They are treated more as advisors than active part in the decision-making process. We reveal different insider sources of information as forming social ties with the CEO and/or inside board directors and collaboration with Audit ＆ Supervisory Board （Kansayaku）, which can help reduce this asymmetry and improve the decision-making process. We assume that it will be easier for the outsiders to establish contacts and form social ties with the Audit ＆ Supervisory Board members because of their unspoken lower status and thus to obtain more information about the company internal affairs and discussions that take place during the informal meetings, where only insiders （including the CEO） are present.
基金Natural Science Foundation of Shandong Province,No.ZR2020QH185Scientific Research Nurturing Fund of The First Affiliated Hospital of Shandong First Medical University&Shandong Provincial Qianfoshan Hospital,No.QYPY2020NSFC0803+2 种基金Guangxi Zhuang Autonomous Region Health Commission Scientific Research Project,No.Z-A20220415Guangxi Medical University Teacher Teaching Ability Development Project,No.2022JFA02Guangxi Medical University Undergraduate Education and Teaching Reform Project,No.2023Y05.
文摘BACKGROUND Colorectal cancer(CRC)is the third most common cancer globally,causing over 900000 deaths annually.Risk factors include aging,diet,obesity,sedentary lifestyle,tobacco use,genetic predisposition,and inflammatory bowel disease.Despite current treatments,survival rates for advanced CRC remain low,highlighting the need for better therapeutic strategies.AIM To evaluate both the clinical significance and the pathological implications of the Kinesin family member 14(KIF14)expression within CRC specimens.Additionally,this study aims to investigate the interaction between nitidine chloride(NC)and KIF14,considering their potential as therapeutic targets.METHODS The expression of the KIF14 protein in CRC was analyzed using immunohistochemical staining.The integration of multicenter high-throughput data facilitated the calculation of the standardized mean difference(SMD)for KIF14 mRNA levels.The assessment of clinical and pathological impact was enhanced by analyzing combined receiver operating characteristic curves,along with measures of sensitivity,specificity,and likelihood ratios.Additionally,clustered regularly interspaced short palindromic repeats knockout screening for cell growth and single-cell sequencing were employed to validate the significance of KIF14 expression in CRC.Survival analysis established the prognostic value of KIF14 in CRC.The molecular mechanism of NC against CRC was elucidated through whole-genome sequencing and enrichment analysis,and molecular docking was utilized to explore the targeting affinity between NC and KIF14.RESULTS KIF14 was highly expressed in 208 CRC patients.Data from 17 platforms involving 2436 CRC samples and 1320 noncancerous colorectal tissue controls indicated that KIF14 expression was significantly higher in CRC samples,with an SMD of 1.92(95%CI:1.49-2.35).The area under the curve was 0.94(95%CI:0.92-0.96),with a sensitivity of 0.85(95%CI:0.78-0.90)and a specificity of 0.90(95%CI:0.85-0.93).The positive and negative likelihood ratios were 8.38(95%CI:5.39-13.02)and 0.17(95%CI:0.11-0.26),respectively.At the single-cell level,significant overexpression of KIF14 was observed in CRC cells(P<0.001),with 35 CRC cell lines dependent on KIF14 for growth.The K-M plots demonstrated that KIF14 possesses prognostic value in CRC patients within the GSE71187 and GSE103679 datasets(P<0.05).Binding energy calculations indicated that KIF14 is a potential target for NC(binding energy:10.3 kcal/mol).CONCLUSION KIF14 promotes the growth of CRC cells and acts as an oncogenic factor,potentially serving as a therapeutic target for NC in the treatment of CRC.
文摘In accordance with the organizational development process and management of party members, in this article it is based on the C/S and B/S mixed structure to finish the application design of client and servers, and using the language of VB.NET develop with Inner Mongolia Agricultural University, members of University College Digital Management Information System. The system from research projects of Agriculture University Occupation Technical College of Inner Mongolia.
基金sponsored by the National Natural Science Foundation of China,China(No.42272171)National Science and Technology Major Project of China,China(No.2017ZX05036).
文摘Dongyuemiao Member shale in the Sichuan Basin,China,is characterized by organic-rich shale intervals with different types of interbeds and accumulation modes.The aim of this study is to elucidate the impact of paleoenvironmental indicators on interbed development.With this aim in mind,we established an interbed classification scheme and quantified the development of different types of interbeds and their frequencies.We categorized the shale interbeds into three types based on interbed type:silt interbeds(SIs),shell fragment interbeds(SFIs),and shell skeleton interbeds(SSIs).The SIs,SFIs,and SSIs are respectively the products of extrabasinal low-density turbidity currents,intrabasinal debris flow,and intrabasinal low-density turbidity currents.We propose that variations in paleoenvironmental conditions primarily influenced the types of interbeds that developed but had minimal impact on the frequency of their development.Models depicting the interbed development within the 1st Submember of Dongyuemiao Member indicate that during the early Dongyuemiao depositional period,under conditions of relatively aridity,weak weathering,high terrigenous input,and strong hydrodynamic activity,SSIs were well developed.In the middle depositional period,as the climate gradually transitioned to more humid conditions,and the weathering intensity and amount of terrestrial input increased,the development of SIs and SFIs significantly increased.During the late depositional period,with a continuous decrease in terrestrial inputs and sedimentation rates,the development of SIs decreased while that of SSIs increased.
基金Supported by the 2023 Government-funded Project of the Outstanding Talents Training Program in Clinical Medicine,No.ZF2023165Key Research and Development Projects of Hebei Province,No.18277731D+1 种基金Natural Science Foundation of Hebei Province,No.H202423105Hebei Provincial Administration of Traditional Chinese Medicine,Scientific Research Project,No.2020014.
文摘In this editorial,we comment on the article by Qin et al,recently published in the World Journal of Gastrointestinal Oncology.Malignant tumors of the digestive tract represent a significant health threat.Kinesin family member 14(KIF14),a critical kinesin,is pivotal in the proliferation,migration,and invasion of tumor cells.It has emerged as a focal point in recent studies of malignant tumors in the digestive tract.This article reviews the current research on KIF14 within these tumors and details its significant role in tumor cell behaviors,including proliferation,apo-ptosis,migration,invasion,and angiogenesis,alongside the regulatory mechanisms of the associated intracellular signaling pathways.Additionally,it explores the clinical value of KIF14 as a potential biomarker for early diagnosis,disease monitoring,and prognostic evaluation in malignant tumors of the digestive tract.The article concludes by introducing the potential regulatory role of traditional Chinese medicine,aiming to combine the strengths of both modern and traditional medical approaches to enhance treatment outcomes and prognosis for patients with these tumors.
基金Supported by the National Natural Science Foundation of China(42302141).
文摘Taking the second member of the Xujiahe Formation of the Upper Triassic in the Xinchang structural belt as an example,based on data such as logging,production,seismic interpretation and test,a systematic analysis was conducted on the structural characteristics and evolution,reservoir diagenesis and densification processes,and types and stages of faults/fractures,and revealing the multi-stage and multi-factor dynamic coupled enrichment mechanisms of tight gas reservoirs.(1)In the early Yanshan period,the paleo-structural traps were formed with low-medium maturity hydrocarbons accumulating in structural highs driven by buoyancy since reservoirs were not fully densified in this stage,demonstrating paleo-structure control on traps and early hydrocarbon accumulation.(2)In the middle-late Yanshan period,the source rocks became mature to generate and expel a large quantity of hydrocarbons.Grain size and type of sandstone controlled the time of reservoir densification,which restricted the scale of hydrocarbon charging,allowing for only a small-scale migration through sand bodies near the fault/fracture or less-densified matrix reservoirs.(3)During the Himalayan period,the source rocks reached overmaturity,and the residual oil cracking gas was efficiently transported along the late-stage faults/fractures.Wells with high production capacity were mainly located in Type I and II fault/fracture zones comprising the late-stage north-south trending fourth-order faults and the late-stage fractures.The productivity of the wells was controlled by the transformation of the late-stage faults/fractures.(4)The Xinchang structural belt underwent three stages of tectonic evolution,two stages of reservoir formation,and three stages of fault/fractures development.Hydrocarbons mainly accumulated in the paleo-structure highs.After reservoir densification and late fault/fracture adjustment,a complex gas-water distribution pattern was formed.Thus,it is summarized as the model of“near-source and low-abundance hydrocarbon charging in the early stage,and differential enrichment of natural gas under the joint control of fault-fold-fracture complex,high-quality reservoirs and structural highs in the late stage”.Faults/fractures with well-coupled fault-fold-fracture-pore are favorable exploration targets with high exploration effectiveness.
文摘At 4:50 on April 30, China's LM-3B/I rocket, an improved type based on LM-3B, made its debut at the Xichang Satellite Launch Center and successfully sending the 12th and 13th BeiDou Navigation Satellite System satellites into the planned transfer orbit in space. It was the first time that China launched two BeiDou satellites with one rocket. It was
基金supported by the German Research Council(Deutsche Forschungsgemeinschaft,HA3309/3-1/2,HA3309/6-1,HA3309/7-1)。
文摘Skeletal muscles are essential for locomotion,posture,and metabolic regulation.To understand physiological processes,exercise adaptation,and muscle-related disorders,it is critical to understand the molecular pathways that underlie skeletal muscle function.The process of muscle contra ction,orchestrated by a complex interplay of molecular events,is at the core of skeletal muscle function.Muscle contraction is initiated by an action potential and neuromuscular transmission requiring a neuromuscular junction.Within muscle fibers,calcium ions play a critical role in mediating the interaction between actin and myosin filaments that generate force.Regulation of calcium release from the sarcoplasmic reticulum plays a key role in excitation-contraction coupling.The development and growth of skeletal muscle are regulated by a network of molecular pathways collectively known as myogenesis.Myogenic regulators coordinate the diffe rentiation of myoblasts into mature muscle fibers.Signaling pathways regulate muscle protein synthesis and hypertrophy in response to mechanical stimuli and nutrient availability.Seve ral muscle-related diseases,including congenital myasthenic disorders,sarcopenia,muscular dystrophies,and metabolic myopathies,are underpinned by dys regulated molecular pathways in skeletal muscle.Therapeutic interventions aimed at preserving muscle mass and function,enhancing regeneration,and improving metabolic health hold promise by targeting specific molecular pathways.Other molecular signaling pathways in skeletal muscle include the canonical Wnt signaling pathway,a critical regulator of myogenesis,muscle regeneration,and metabolic function,and the Hippo signaling pathway.In recent years,more details have been uncovered about the role of these two pathways during myogenesis and in developing and adult skeletal muscle fibers,and at the neuromuscular junction.In fact,research in the last few years now suggests that these two signaling pathways are interconnected and that they jointly control physiological and pathophysiological processes in muscle fibers.In this review,we will summarize and discuss the data on these two pathways,focusing on their concerted action next to their contribution to skeletal muscle biology.However,an in-depth discussion of the noncanonical Wnt pathway,the fibro/a dipogenic precursors,or the mechanosensory aspects of these pathways is not the focus of this review.
文摘Fibre and fabric production technologies-especially in the area of com-posite reinforcements-have played an as-yet largely unheralded role in thedevelopment of the UKs Formula One industry,but the British Textile MachineryAssociation(BTMA)aims to change that.
文摘Published:25 September 2025 In the article“MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2”(BIOCELL,2021,Vol.45,No.3,pp.627-638.doi:10.32604/biocell.2021.013496),there is an error in the Fig.3.In order to ensure the scientific and rigorous nature of our academic publications,we made a correction about Fig.3 correspondingly.
基金Supported by Wuxi Institute of Translational Medicine Project Program,No.LCYJ202336the Scientific and Technological Achievements Promotion Project of Wuxi Municipal Health Commission Project Program,No.T202336+1 种基金Hospital Management Innovation Research Project of Jiangsu Hospital Association,No.JSYGY-3-2024-601Jiangsu Provincial Traditional Chinese Medicine Science and Technology Development Plan Project,No.MS2024063。
文摘BACKGROUND Grief counseling has become relatively established and is widely used among the families of cancer patients,effectively alleviating their psychological pain.However,in China,due to the influence of Confucianism and other traditional cultures,people generally adhere to the belief of“reincarnation to avoid death”,focusing more on themes of life,such as eugenics and longevity,and paying less attention to matters related to death,including death education and grief counseling.Currently,grief counseling in China is still in an exploratory stage,and there is relatively little research on the psychological status of family members of patients with terminal tumors.AIM To investigate the psychological effects of grief counseling on family members of terminal cancer patients.METHODS This study was designed as a randomized controlled trial that utilized convenience sampling to select family members of terminal tumor patients who were admitted to the hospice ward of the Affiliated Hospital of Jiangnan University from January to June 2025 as research subjects.All participants received conventional symptomatic supportive treatment and palliative care.Additionally,the intervention group benefited from extra grief counseling.RESULTS The Distress Thermometer(DT)score of the control group slightly decreased compared to before the intervention,but the difference was not statistically significant(P>0.05).In contrast,the DT score of the intervention group decreased significantly compared to before the intervention,with a statistically significant difference(P<0.05).After the intervention,the DT score of the intervention group was lower than that of the control group,and the difference was also statistically significant(P<0.05).After the intervention,the intervention group performed better DT level than the control group,with a statistically significant difference(P<0.05),the intervention group outperformed the control group in terms of depression and anxiety,and the difference was statistically significant(P<0.05).CONCLUSION Grief counseling can help alleviate the psychological pain and negative emotions experienced by family members of patients with terminal malignant tumors.
基金supported by the National Key Research and Development Program[grant number 2022YFC3004203]the S&T Development Fund of CAMS(Chinese Academy of Meteorological Sciences)[grant numbers 2023KJ040 and 2024KJ013].
文摘Subseasonal forecasting of extreme events is crucial for early warning systems.However,the forecast skills for extreme events are limited.Taking the extreme cold events in January 2018 as a specific example,and analyzing the 34 extreme cold events in East Asia from 1998 to 2020,the authors evaluated the forecast skills of the ECMWF model ensemble members on subseasonal time scales.The results show that while the ensemble mean has limited skills for forecasting extreme cold events at the 3-week lead time,some individual members demonstrate high forecast skills.For most extreme cold events,there are>10%of members among the total ensembles that can well predict the rapid temperature transitions at the 14-day lead time.This highlights the untapped potential of the ECMWF model to forecast extreme cold events on subseasonal time scales.High-skill ensemble members rely on accurate predictions of atmospheric circulation patterns(500-hPa geopotential height,mean sea level pressure)and key weather systems,including the Ural Blocking and Siberian High,that influence extreme cold events.
基金Supported by Jinchang City Level Science and Technology Plan Project(No.2023RC012).
文摘The lower member of Dalazi Formation is an important oil reservoir in the Songjiang Basin.Based on the research on its field-measured geological profiles,lithological combination characteristics and grain size distribution characteristics,combined with the analysis of the spatial distribution characteristics,sedimentary structural characteristics and hydrodynamic conditions of the sediments in this member,nine sedimentary microfacies of the fan delta plain subfacies,fan delta front subfacies and littoral-shallow lacustrine subfacies have been identified.The study reveals that lower member of Dalazi Formation in the research area follows a fan delta–shallow lacustrine depositional model in the steep slope zone of a rift lake basin.The sediments primarily originate from the Pre-Mesozoic strata in the steep southeastern and eastern margins.The basin center migrated from the early Xiaoshahe area to the Yangmucun–Shenglicun area,and the sedimentary system gradually transitioned from fan delta to littoral-shallow lacustrine facies.
基金supported by the National Natural Science Foundation of China:Tethys Dynamics(Grant No.92255302)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDA14010201)the China Scholarship Council(Grant No.202206400023).
文摘Understanding diagenetic processes plays a crucial role in evaluating the quality of tight reservoirs.In this study,we aimed to examine diagenetic facies and thereby assess reservoir quality by conducting an integrated analysis of porosity,petrology,permeability,mineralogy,mercury injection,and stable isotopic data in core samples from the Permian Shan 2 and He 1 members(Shan 2—He 1 Mbr)in China's Ordos Basin.Early compaction and precipitation of diagenetic minerals have significantly reduced primary pore space in these members,although certain sandstones have retained anomalously high porosity.These high-porosity reservoirs have been shaped by many factors,with dissolution identified as the predominant mechanism.Detailed petrographic observations and assessments of the primary minerals,the minerals'genesis,and associated diagenetic processes revealed seven distinct diagenetic facies differentiated by their sand-grain populations,types of cementation,clay matrix contents,and volcanic tuffaceous sediment(VTS).The main interstitial fillings consist of VTS,clay minerals,quartz overgrowths,and calcite(ferrocalcite).Clasts and VTS-dissolved sandstone have mainly formed Class I reservoirs with highly unstable grains and good secondary pores resulting from the dissolution of the grains and VTS.VTS tight sandstone,siliceous cementation tight sandstone,illite-siliceous cementation tight sandstone,and calcite cementation tight sandstone have formed Class II reservoirs with high rates of interstitial filling and cementation.Sandstone tightly cemented by illite has poor reservoir properties and has mainly formed Class III reservoirs.This classification underscores the critical importance of understanding diagenesis,diagenetic facies,and especially the diagenetic features of VTS for accurately assessing reservoir quality.A comprehensive understanding of these factors may facilitate more effective hydrocarbon exploration and exploitation in this area.
基金Supported by National Natural Science Foundation of China,No.31640052and Natural Science Foundation of Shandong Province,China,No.ZR2019BH031.
文摘BACKGROUND Aberrant microRNAs expression and associated pathways have been proved participate in regulation vast various physiologic and pathologic processed of different human cancers including liver cancer.While,the function of miR-451a in liver cancer still indistinct.AIM To study the effect of miR-451a in liver cancer development.METHODS GeneChip microarray analysis performed to detect miR-451a expression in liver cancer tissues and normal liver tissues.Reverse transcription-polymerase chain reaction was used to validate the expression of miR-451a in liver cancer cell and other tumor cell lines.Construction of liver cancer cell lines that stably overexpressed miR-451a by transfecting Lentivirus produced by Genechem company.Methylthiazolyldiphenyl-tetrazolium(MTT)bromide assay and colony formation assay to determine the effect of miR-451a in liver cancer cell proliferation.Flow cytometry used to investigate whether miR-451a involved in liver cancer cell apoptosis.Cell migration ability was measured via wound scratch assay.Target gene was explored by bioinformatic analysis,and downstream molecule of miR-451a in liver cancer identified by rescue experiments.RESULTS MiR-451a expression significantly downregulation in liver cancer tissues compared with that in normal liver tissue.MiR-451a also obviously low-expressed in liver cancer cell,colorectal carcinoma cell and esophageal carcinoma cell lines.Human hepatoblastoma G2(HepG2)and BEL-7404 cell lines that stably overexpressed miR-451a by transfecting lentivirus constructed successfully.MTT bromide assay and colony formation assay showed that the overexpressed miR-451a inhibit HepG2 cell proliferation viability,but not BEL-7404 cell.Flow cytometry determined that miR-451a regulating proliferation not through inducing apoptosis.Wound scratch assay revealed that miR-451a overexpression suppressed HepG2 cell migration.Furthermore,mex-3 RNA binding family member C was predicted as the target gene by bioinformatic analysis,and rescue experiments confirmed the hypothesis.CONCLUSION Therefore,miR-451a may be candidate miRNA for understanding molecular mechanisms of liver cancer development and novel target in liver cancer cell.
