This research aimed to develop a prototype process for hydrogen production from Napier grass(NG)and oil palm frond(OPF).First,biohydrogen production was investigated in batch experiments using either the simultaneous ...This research aimed to develop a prototype process for hydrogen production from Napier grass(NG)and oil palm frond(OPF).First,biohydrogen production was investigated in batch experiments using either the simultaneous saccharification and fermentation(SSF)process or the pre-hydrolysis and fermentation(pre-HF)process under different NG to OPF ratios.The proportions of NG:OPF of 2.5:7.5,5.0:5.0,and 7.5:2.5 g-VS/L were tested in both SSF and pre-HF.The results showed that the proportion of NG:OPF of 5.0:5.0 g-VS/L(ratio of 1:1)had the highest hydrogen production in both the SSF and pre-HF processes,with hydrogen production of 370±21 mL-H_(2)/L and 993±64 mL-H_(2)/L,respectively.Among these,pre-HF showed higher performance.Next,a semicontinuous experiment in a 100 L continuous stirred tank reactor(CSTR)with a pre-HF process and NG:OPF of 5.0:5.0 g-VS/L at hydraulic retention times(HRTs)of 4 and 3 days,with and without trace metal supple-mentation,was conducted.The highest hydrogen yield,50.1±3.4 mL-H_(2)/g-VS,was found at an HRT of 3 days with trace metal supplementation.Key microbes,including Lactococcus sp.,Bacteroides sp.,Dysgonomonas sp.,and Enterobacter sp.,showed increased abundance,improving hydrogen production from NG and OPF.The addition of trace elements significantly altered essential enzymes like 6-phosphofructokinase,which is crucial for forming pyruvate in hydrogen production.The medium-based economic analysis revealed that the pre-HF process with trace elements resulted in an overall hydrogen production of 9.32 m3,an improvement of 16.6% compared to the 7.99 m3 obtained under pre-HF without adding trace elements.Consequently,the Economic Yield(EY)of pre-HF without trace element addition was 0.030 USD-H_(2)/USD-medium,which was enhanced to 0.035 USD-H_(2)/USD-medium by adding trace elements.展开更多
基金co-funded by the National Research Council of Thailand(NRCT)and the Electricity Generating Authority of Thailand(EGAT)(Grant No.N25D650029)partially supported by the National Research Council of Thailand(NRCT)(Grant No.N42A670487).
文摘This research aimed to develop a prototype process for hydrogen production from Napier grass(NG)and oil palm frond(OPF).First,biohydrogen production was investigated in batch experiments using either the simultaneous saccharification and fermentation(SSF)process or the pre-hydrolysis and fermentation(pre-HF)process under different NG to OPF ratios.The proportions of NG:OPF of 2.5:7.5,5.0:5.0,and 7.5:2.5 g-VS/L were tested in both SSF and pre-HF.The results showed that the proportion of NG:OPF of 5.0:5.0 g-VS/L(ratio of 1:1)had the highest hydrogen production in both the SSF and pre-HF processes,with hydrogen production of 370±21 mL-H_(2)/L and 993±64 mL-H_(2)/L,respectively.Among these,pre-HF showed higher performance.Next,a semicontinuous experiment in a 100 L continuous stirred tank reactor(CSTR)with a pre-HF process and NG:OPF of 5.0:5.0 g-VS/L at hydraulic retention times(HRTs)of 4 and 3 days,with and without trace metal supple-mentation,was conducted.The highest hydrogen yield,50.1±3.4 mL-H_(2)/g-VS,was found at an HRT of 3 days with trace metal supplementation.Key microbes,including Lactococcus sp.,Bacteroides sp.,Dysgonomonas sp.,and Enterobacter sp.,showed increased abundance,improving hydrogen production from NG and OPF.The addition of trace elements significantly altered essential enzymes like 6-phosphofructokinase,which is crucial for forming pyruvate in hydrogen production.The medium-based economic analysis revealed that the pre-HF process with trace elements resulted in an overall hydrogen production of 9.32 m3,an improvement of 16.6% compared to the 7.99 m3 obtained under pre-HF without adding trace elements.Consequently,the Economic Yield(EY)of pre-HF without trace element addition was 0.030 USD-H_(2)/USD-medium,which was enhanced to 0.035 USD-H_(2)/USD-medium by adding trace elements.
