[目的]丙泊酚改善重症脓毒症小鼠脑保护作用的研究。[方法]将48只雄性小鼠随机分为对照组、LPS组、PF组、PF+ML385组,每组12只。通过苏木素-伊红染色评估各组小鼠海马组织的病理变化;通过原位末端标记法分析海马组织中神经元的凋亡率;...[目的]丙泊酚改善重症脓毒症小鼠脑保护作用的研究。[方法]将48只雄性小鼠随机分为对照组、LPS组、PF组、PF+ML385组,每组12只。通过苏木素-伊红染色评估各组小鼠海马组织的病理变化;通过原位末端标记法分析海马组织中神经元的凋亡率;通过酶联免疫吸附实验分析血清中TNF-α、IL-6、SOD和CAT的水平;通过蛋白免疫印迹实验分析海马组织中TLR4/MD-2的蛋白表达水平。[结果]与LPS组比较,PF组与PF+ML385组小鼠海马组织区域炎症细胞聚集减少,神经元损伤减轻,神经元凋亡率降低,血清炎症因子(TNF-α和IL-6)水平降低,血清抗氧化酶(SOD和CAT)活性水平增加,海马组织的TLR4、MD-2蛋白表达水平降低。与PF组比较,PF+ML385组小鼠海马组织神经元凋亡率降低(13.26%±2.19%vs 9.28%±1.58%;P<0.05),血清炎症因子(TNF-α、IL-6)水平降低(15.61±1.29 vs 10.28±1.46 pg/mL;30.28±1.11 vs 23.56±2.13 pg/mL;P<0.05),血清抗氧化酶(SOD、CAT)活性水平增加(13.69±1.72 vs 19.23±2.39 U/mg protein;12.97±2.26 vs 17.38±1.47 U/mg protein;P<0.05),海马组织的TLR4、MD-2蛋白表达水平降低(0.65±0.03 vs 0.33±0.09;0.72±0.08 vs 0.41±0.04;P<0.05)。[结论]丙泊酚能够改善脓毒症小鼠海马组织的神经元损伤,减少神经元凋亡率和炎症因子水平,增加抗氧化酶水平。丙泊酚的这一作用与抑制TLR4/MD-2通路密切相关。展开更多
【目的】系统探究柴达木盆地耕地耕层土壤盐碱化水平及养分状况,优化柴达木盆地耕地耕层土壤评价因子,构建评价与诊断模型,为区域粮食安全和生态可持续发展提供科学依据。【方法】以柴达木盆地耕作层土壤为对象,基于470个土壤样品的11...【目的】系统探究柴达木盆地耕地耕层土壤盐碱化水平及养分状况,优化柴达木盆地耕地耕层土壤评价因子,构建评价与诊断模型,为区域粮食安全和生态可持续发展提供科学依据。【方法】以柴达木盆地耕作层土壤为对象,基于470个土壤样品的11项土壤指标(全盐:TS、电导率:EC、全氮:TN、全磷:TP、速效氮:AN、速效磷:AP、pH、有机质:SOM、钠离子:Na^(+)、氯离子:Cl^(-)、硫酸根离子:SO_(4)^(2-)),通过主成分分析(PCA)与Norm值构建最小数据集(Minimum data set,MDS),结合多准则妥协解排序法(VIKOR法),系统评价土壤质量指数(Soil quality index,SQI)并诊断主要障碍因子。【结果】研究区土壤呈现显著盐碱化风险(TS均值2.449 g/kg,C_(V)=53.98%)和氮磷养分限制特征(TN均值为0.916 mg/kg,TP的C_(V)为118.38%);MDS筛选出TS、TN、TP、AP、Cl^(-)和SO_(4)^(2-)等6项核心指标,可替代全数据集(Total data set,TDS)精准评价土壤质量(R^(2)=0.904);VIKOR模型显示,格尔木和大柴旦地区土壤综合障碍度最高(群体效应值S>0.46),TP与SO_(4)^(2-)是主要限制因子,德令哈土壤质量最优(SQI=0.284)。【讨论】研究揭示了盐碱化与养分失衡对干旱区土壤质量的协同制约效应,为柴达木盆地耕地定向改良提供了科学依据。展开更多
It is important to understand the effects of demineralization on the reactivity of coal pyrolysis for demineralization coal in OCCSS systems.The molecular dynamics of the pyrolysis of ZD-R and ZD-D was investigated us...It is important to understand the effects of demineralization on the reactivity of coal pyrolysis for demineralization coal in OCCSS systems.The molecular dynamics of the pyrolysis of ZD-R and ZD-D was investigated using the ReaxFF MD method.It is indicated that the pyrolysis reactivity of ZD-D is enhanced.Acid demineralization treatment helps to enhance the cross-linking reaction of light tar C5-14 molecules in the main pyrolysis stage,generating light tar fragments with higher molecular mass.In the polycondensation pyrolysis stage,the acid demineralization treatment promotes the polycondensation reaction at high temperatures.The generation of H2 in ZD-R pyrolysis is primarily attributed to the N-H bond breaking in pyrrole at low temperatures.At high temperatures,it is predominantly associated with the hydrogen abstraction reaction between carboxyl functional groups and the dehydrogenation reaction of hydrogen bond breaking in the aliphatic hydrocarbon structures.H2O is primarily attributed to the reaction of the hydroxyl group on the carboxyl group.CO_(2) is always related to the carboxyl group.CO is mainly related to the reduction reaction of carbonyl group,carboxyl group,and CO_(2).During the initial pyrolysis stage of ZD-D after acid demineralization treatment,the hydrogen abstraction reaction of the carboxyl functional group and the generation of CO molecules by the methoxy group are enhanced.The generation of H2 by the aliphatic structure is enhanced in the main pyrolysis stage.The ring-opening reaction of the phenoxy group to generate CO in the polycondensation pyrolysis stage is also facilitated.展开更多
以‘嘎拉’苹果为材料,克隆了6–磷酸葡萄糖酸脱氢酶基因Md6PGDH1(序列号:MDP0000279299)全长。序列分析显示,该基因包含一个长为1 047 bp完整的开放阅读框,编码347个氨基酸,分子量为36.430 k D,预测等电点为9.24。同源性分析表明Md6PG...以‘嘎拉’苹果为材料,克隆了6–磷酸葡萄糖酸脱氢酶基因Md6PGDH1(序列号:MDP0000279299)全长。序列分析显示,该基因包含一个长为1 047 bp完整的开放阅读框,编码347个氨基酸,分子量为36.430 k D,预测等电点为9.24。同源性分析表明Md6PGDH1还有另外3个同源基因;功能域分析表明Md6PGDH蛋白含有两个保守的绑定域;亚细胞预测表明Md6PGDH定位存在差异。分析Md6PGDH1启动子发现存在多个响应非生物胁迫的顺式作用元件。定量分析显示,Md6PGDH1在苹果的不同组织中都有表达,且受非生物胁迫诱导。原核诱导Md6PGDH1蛋白并进行蛋白酶活的测定,为后续蛋白功能鉴定奠定了基础。Md6PGDH1在苹果愈伤组织中过量表达,提高其抗盐胁迫的能力。展开更多
S100 proteins govern cellular proliferation,differentiation,apoptosis,calcium homeostasis,energy metabolism,and inflammation.This heterodimeric calcium-binding protein,consisting of the S100A8 and S100A9 subunits,modu...S100 proteins govern cellular proliferation,differentiation,apoptosis,calcium homeostasis,energy metabolism,and inflammation.This heterodimeric calcium-binding protein,consisting of the S100A8 and S100A9 subunits,modulates myeloid cell development.展开更多
In-situ TEM observation was conducted during Ni^(+)&He^(+)dual-beam irradiation to monitor the evolution of dislocation loops accompanied by He bubbles in the Ni-based alloy GH3535.Two distinct evolutions of dislo...In-situ TEM observation was conducted during Ni^(+)&He^(+)dual-beam irradiation to monitor the evolution of dislocation loops accompanied by He bubbles in the Ni-based alloy GH3535.Two distinct evolutions of dislocation loops,driven by residual stresses,were observed within the monitored grains.Hence,molec-ular dynamics(MD)simulations were employed to reveal the effects of stress magnitude and direction on loop evolution,including size,number density,type and variation.The simulations revealed that the presence of compressive stress reduced the formation energy of perfect dislocation loops,thus promoting their formation.Stress state was found to influence the preferential orientation of the loops,and com-pressive stress resulted in a decreased number density of dislocation loops but an increase in their size.This establishes a clear relationship between stress state and magnitude and the evolution of dislocation loops during ion beam irradiation.Additionally,the nature and characteristics of dislocation loops were quantified to explore the effects of He concentrations on their evolution.The higher He concentration not only promotes the nucleation of dislocation loops,leading to their higher number density,but also facil-itates the unfaulting evolution by increasing the stacking fault energy(SFE).Moreover,the accumulation of He in the lower-He-concentration sample led to the growth of dislocation loops in multiple stages,explaining their nearly identical average sizes when compared to the higher-He-concentration sample.展开更多
文摘[目的]丙泊酚改善重症脓毒症小鼠脑保护作用的研究。[方法]将48只雄性小鼠随机分为对照组、LPS组、PF组、PF+ML385组,每组12只。通过苏木素-伊红染色评估各组小鼠海马组织的病理变化;通过原位末端标记法分析海马组织中神经元的凋亡率;通过酶联免疫吸附实验分析血清中TNF-α、IL-6、SOD和CAT的水平;通过蛋白免疫印迹实验分析海马组织中TLR4/MD-2的蛋白表达水平。[结果]与LPS组比较,PF组与PF+ML385组小鼠海马组织区域炎症细胞聚集减少,神经元损伤减轻,神经元凋亡率降低,血清炎症因子(TNF-α和IL-6)水平降低,血清抗氧化酶(SOD和CAT)活性水平增加,海马组织的TLR4、MD-2蛋白表达水平降低。与PF组比较,PF+ML385组小鼠海马组织神经元凋亡率降低(13.26%±2.19%vs 9.28%±1.58%;P<0.05),血清炎症因子(TNF-α、IL-6)水平降低(15.61±1.29 vs 10.28±1.46 pg/mL;30.28±1.11 vs 23.56±2.13 pg/mL;P<0.05),血清抗氧化酶(SOD、CAT)活性水平增加(13.69±1.72 vs 19.23±2.39 U/mg protein;12.97±2.26 vs 17.38±1.47 U/mg protein;P<0.05),海马组织的TLR4、MD-2蛋白表达水平降低(0.65±0.03 vs 0.33±0.09;0.72±0.08 vs 0.41±0.04;P<0.05)。[结论]丙泊酚能够改善脓毒症小鼠海马组织的神经元损伤,减少神经元凋亡率和炎症因子水平,增加抗氧化酶水平。丙泊酚的这一作用与抑制TLR4/MD-2通路密切相关。
文摘【目的】系统探究柴达木盆地耕地耕层土壤盐碱化水平及养分状况,优化柴达木盆地耕地耕层土壤评价因子,构建评价与诊断模型,为区域粮食安全和生态可持续发展提供科学依据。【方法】以柴达木盆地耕作层土壤为对象,基于470个土壤样品的11项土壤指标(全盐:TS、电导率:EC、全氮:TN、全磷:TP、速效氮:AN、速效磷:AP、pH、有机质:SOM、钠离子:Na^(+)、氯离子:Cl^(-)、硫酸根离子:SO_(4)^(2-)),通过主成分分析(PCA)与Norm值构建最小数据集(Minimum data set,MDS),结合多准则妥协解排序法(VIKOR法),系统评价土壤质量指数(Soil quality index,SQI)并诊断主要障碍因子。【结果】研究区土壤呈现显著盐碱化风险(TS均值2.449 g/kg,C_(V)=53.98%)和氮磷养分限制特征(TN均值为0.916 mg/kg,TP的C_(V)为118.38%);MDS筛选出TS、TN、TP、AP、Cl^(-)和SO_(4)^(2-)等6项核心指标,可替代全数据集(Total data set,TDS)精准评价土壤质量(R^(2)=0.904);VIKOR模型显示,格尔木和大柴旦地区土壤综合障碍度最高(群体效应值S>0.46),TP与SO_(4)^(2-)是主要限制因子,德令哈土壤质量最优(SQI=0.284)。【讨论】研究揭示了盐碱化与养分失衡对干旱区土壤质量的协同制约效应,为柴达木盆地耕地定向改良提供了科学依据。
文摘It is important to understand the effects of demineralization on the reactivity of coal pyrolysis for demineralization coal in OCCSS systems.The molecular dynamics of the pyrolysis of ZD-R and ZD-D was investigated using the ReaxFF MD method.It is indicated that the pyrolysis reactivity of ZD-D is enhanced.Acid demineralization treatment helps to enhance the cross-linking reaction of light tar C5-14 molecules in the main pyrolysis stage,generating light tar fragments with higher molecular mass.In the polycondensation pyrolysis stage,the acid demineralization treatment promotes the polycondensation reaction at high temperatures.The generation of H2 in ZD-R pyrolysis is primarily attributed to the N-H bond breaking in pyrrole at low temperatures.At high temperatures,it is predominantly associated with the hydrogen abstraction reaction between carboxyl functional groups and the dehydrogenation reaction of hydrogen bond breaking in the aliphatic hydrocarbon structures.H2O is primarily attributed to the reaction of the hydroxyl group on the carboxyl group.CO_(2) is always related to the carboxyl group.CO is mainly related to the reduction reaction of carbonyl group,carboxyl group,and CO_(2).During the initial pyrolysis stage of ZD-D after acid demineralization treatment,the hydrogen abstraction reaction of the carboxyl functional group and the generation of CO molecules by the methoxy group are enhanced.The generation of H2 by the aliphatic structure is enhanced in the main pyrolysis stage.The ring-opening reaction of the phenoxy group to generate CO in the polycondensation pyrolysis stage is also facilitated.
文摘以‘嘎拉’苹果为材料,克隆了6–磷酸葡萄糖酸脱氢酶基因Md6PGDH1(序列号:MDP0000279299)全长。序列分析显示,该基因包含一个长为1 047 bp完整的开放阅读框,编码347个氨基酸,分子量为36.430 k D,预测等电点为9.24。同源性分析表明Md6PGDH1还有另外3个同源基因;功能域分析表明Md6PGDH蛋白含有两个保守的绑定域;亚细胞预测表明Md6PGDH定位存在差异。分析Md6PGDH1启动子发现存在多个响应非生物胁迫的顺式作用元件。定量分析显示,Md6PGDH1在苹果的不同组织中都有表达,且受非生物胁迫诱导。原核诱导Md6PGDH1蛋白并进行蛋白酶活的测定,为后续蛋白功能鉴定奠定了基础。Md6PGDH1在苹果愈伤组织中过量表达,提高其抗盐胁迫的能力。
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.82170160 and 32000604)the Provincial Natural Science Foundation of Hunan(Grant Nos.2021JJ30163 and 2022JJ20021)+3 种基金the Changsha Municipal Natural Science Foundation(Grant No.kq2007053)the Global Research Award from American Society of Hematology to Y.Mpartially supported by the National Natural Science Foundation of China(Grant No.82470145)Mount Taishan Scholar Young Expert of Shandong Province(Grant No.tsqn202103167)to S.X。
文摘S100 proteins govern cellular proliferation,differentiation,apoptosis,calcium homeostasis,energy metabolism,and inflammation.This heterodimeric calcium-binding protein,consisting of the S100A8 and S100A9 subunits,modulates myeloid cell development.
基金supported by the National Natural Science Foundation of China(Nos.U2341261 and 12375280)the Young Potential Program of Shanghai Institute of Applied Physics,Chinese Academy of Sciences(No.YXJH-2022002)the National MCF Energy R&D Program(No.2022YFE03110000).
文摘In-situ TEM observation was conducted during Ni^(+)&He^(+)dual-beam irradiation to monitor the evolution of dislocation loops accompanied by He bubbles in the Ni-based alloy GH3535.Two distinct evolutions of dislocation loops,driven by residual stresses,were observed within the monitored grains.Hence,molec-ular dynamics(MD)simulations were employed to reveal the effects of stress magnitude and direction on loop evolution,including size,number density,type and variation.The simulations revealed that the presence of compressive stress reduced the formation energy of perfect dislocation loops,thus promoting their formation.Stress state was found to influence the preferential orientation of the loops,and com-pressive stress resulted in a decreased number density of dislocation loops but an increase in their size.This establishes a clear relationship between stress state and magnitude and the evolution of dislocation loops during ion beam irradiation.Additionally,the nature and characteristics of dislocation loops were quantified to explore the effects of He concentrations on their evolution.The higher He concentration not only promotes the nucleation of dislocation loops,leading to their higher number density,but also facil-itates the unfaulting evolution by increasing the stacking fault energy(SFE).Moreover,the accumulation of He in the lower-He-concentration sample led to the growth of dislocation loops in multiple stages,explaining their nearly identical average sizes when compared to the higher-He-concentration sample.
