采用改进的CTAB法提取长柄双花木（ Disanthus cercidifolius var. longipes）基因组DNA，并以此DNA为模板，对ISSR—PCR的反应体系进行了优化，建立了适合长柄双花木ISSR—PCR的反应体系和程序，即20μL的反应体系中，含模板DNA20ng，M...采用改进的CTAB法提取长柄双花木（ Disanthus cercidifolius var. longipes）基因组DNA，并以此DNA为模板，对ISSR—PCR的反应体系进行了优化，建立了适合长柄双花木ISSR—PCR的反应体系和程序，即20μL的反应体系中，含模板DNA20ng，Mg2^＋2．0mmol·L^-1，引物0．25μmol·L^-1，dNTPs0．20mmol·L^-1，Taq DNA聚合酶1．0U；扩增程序：94℃预变性5min，94℃变性30S，48—54℃复性45s；72℃延伸90s；共36个循环；循环结束后，72℃延伸7min．本研究为利用这一分子标记对长柄双花木进行遗传多样性分析奠定了基础．展开更多
Effects of treating with 0.05, 0.10, 0.20 and 0.40 mg·g-1 NAA for 3 h on cutting regeneration of Disanthus cercidifolius var. longipes H.T.Chang were studied. The results indicated that effects of different conce...Effects of treating with 0.05, 0.10, 0.20 and 0.40 mg·g-1 NAA for 3 h on cutting regeneration of Disanthus cercidifolius var. longipes H.T.Chang were studied. The results indicated that effects of different concentrations of NAA on biological indexes of cutting regeneration weremarkedly different except plantheight and ground diameter.With0.1 mg·g-1 NAA solution treating for 3 h, individual biomass, root biomass, total length of root, root number, new leaf number and total length of new branch increased obviously.展开更多
Effect of essential oil from Asarum heterotropoides Fr.Schmidt var.mandshuricum(Maxim.)Kitag on hypha growth of sixteen plant pathogenic fungi was tested at concentration of 500 μg/mL.The inhibition rates to fourteen...Effect of essential oil from Asarum heterotropoides Fr.Schmidt var.mandshuricum(Maxim.)Kitag on hypha growth of sixteen plant pathogenic fungi was tested at concentration of 500 μg/mL.The inhibition rates to fourteen plant pathogenic fungi were above 50%,which showed the essential oil had broad-spectrum antifungal activity.According to the toxicity curve of essential oil to Alternaria humicola,the EC50 to mycelium growth and spores germination were 326.86 μg/mL and 406.24 μg/mL respectively.Furthermore,the effect of essential oil on cell membrane potential of A.humicola was tested by the conductivity method.The results showed the action of essential oil was changing osmosis of cell membrane of fungi.展开更多
文摘Effects of treating with 0.05, 0.10, 0.20 and 0.40 mg·g-1 NAA for 3 h on cutting regeneration of Disanthus cercidifolius var. longipes H.T.Chang were studied. The results indicated that effects of different concentrations of NAA on biological indexes of cutting regeneration weremarkedly different except plantheight and ground diameter.With0.1 mg·g-1 NAA solution treating for 3 h, individual biomass, root biomass, total length of root, root number, new leaf number and total length of new branch increased obviously.
文摘Effect of essential oil from Asarum heterotropoides Fr.Schmidt var.mandshuricum(Maxim.)Kitag on hypha growth of sixteen plant pathogenic fungi was tested at concentration of 500 μg/mL.The inhibition rates to fourteen plant pathogenic fungi were above 50%,which showed the essential oil had broad-spectrum antifungal activity.According to the toxicity curve of essential oil to Alternaria humicola,the EC50 to mycelium growth and spores germination were 326.86 μg/mL and 406.24 μg/mL respectively.Furthermore,the effect of essential oil on cell membrane potential of A.humicola was tested by the conductivity method.The results showed the action of essential oil was changing osmosis of cell membrane of fungi.
